Estudio comparativo de la composición y actividad biológica de los aceites esenciales extraídos de lippia alba, lippia origanoides y phyla dulcis, especies de la familia verbenaceae

Se determinó por GC -MS la composición química de los aceites esenciales de dos quimiotipos de Lippia alba, citral y carvona; Lippia origanoides y Phyla dulcis. Se evaluó la actividad antioxidante de cada uno de los aceites, como una medida de la capacidad antirradicalaria. Adicionalmente, se evaluaron la citotoxicidad en células Vero y las actividades antifúngica, antiparasitaria y antibacterial. Los resultados obtenidos permitieron establecer que el aceite de L. origanoides, fue la mezcla más promisoria , debido a su alta actividad en los diferentes ensayos biológicos, y su capacidad antirradicalaria, y a ser un aceite esencial no tóxico

Estudio comparativo de la composición y actividad biológica de los aceites esenciales extraídos de lippia alba, lippia origanoides y phyla dulcis, especies de la familia verbenaceae

Se determinó por GC -MS la composición química de los aceites esenciales de dos quimiotipos de Lippia alba, citral y carvona; Lippia origanoides y Phyla dulcis. Se evaluó la actividad antioxidante de cada uno de los aceites, como una medida de la capacidad antirradicalaria. Adicionalmente, se evaluaron la citotoxicidad en células Vero y las actividades antifúngica, antiparasitaria y antibacterial. Los resultados obtenidos permitieron establecer que el aceite de L. origanoides, fue la mezcla más promisoria , debido a su alta actividad en los diferentes ensayos biológicos, y su capacidad antirradicalaria, y a ser un aceite esencial no tóxico

Estudio comparativo de la composición y actividad biológica de los aceites esenciales extraídos de lippia alba, lippia origanoides y phyla dulcis, especies de la familia verbenaceae

Se determinó por GC -MS la composición química de los aceites esenciales de dos quimiotipos de Lippia alba, citral y carvona; Lippia origanoides y Phyla dulcis. Se evaluó la actividad antioxidante de cada uno de los aceites, como una medida de la capacidad antirradicalaria. Adicionalmente, se evaluaron la citotoxicidad en células Vero y las actividades antifúngica, antiparasitaria y antibacterial. Los resultados obtenidos permitieron establecer que el aceite de L. origanoides, fue la mezcla más promisoria , debido a su alta actividad en los diferentes ensayos biológicos, y su capacidad antirradicalaria, y a ser un aceite esencial no tóxico

Dengue en el embarazo: efectos en el feto y el recién nacido.

The risk of dengue virus infection during pregnancy has increased due to the current rash of frequent and severe dengue epidemics. The effects of dengue virus in the fetus and newborn children have been studied only superficially and with contradictory results. Therefore, a retrospective cohort study was conducted in Medellin, Colombia, to describe the fetal and postnatal effects of dengue virus infection acquired during pregnancy. Twenty-two babies born from mothers who suffered dengue during the epidemics of 1998 were compared with babies from non-infected mothers. In the exposed cohort, three premature births occurred, three children suffered from fetal anomalies and four children were born with low weight. In the non-exposed children, none of these problems were found. Psychomotor development was normal in both groups. Only the low weight subgroup was statistically significant (Fisher test, p = 0.045). These results suggested that the children from women with dengue during pregnancy present low weight, greater frequency of premature birth and increased fetal distress. A larger sample is necessary to confirm these results.El riesgo de infección por el virus del dengue durante el embarazo se está incrementando ante mayores y más severas epidemias, y las consecuencias sobre el feto y el recién nacido han sido poco estudiadas y, en otros casos, los resultados han sido contradictorios. Por esta razón, se realizó en Medellín un estudio de cohorte retrospectiva, cuyo objetivo fue determinar los efectos que produce el dengue durante el embarazo sobre el feto y el recién nacido. En dicho estudio se evaluaron 22 recién nacidos hijos de mujeres que presentaron dengue durante la epidemia de 1998 y se compararon con 24 recién nacidos, hijos de mujeres embarazadas sin dengue. En la cohorte con dengue se encontraron 3 niños prematuros, 3 niños con sufrimiento fetal y 4 niños con bajo peso al nacer. En la cohorte no expuesta no se encontraron niños con estos problemas. El desarrollo psicomotor fue normal en ambos grupos. De las observaciones anteriores, sólo fue estadísticamente significativa la frecuencia de niños con bajo peso al nacer (prueba exacta de Fisher, p=0,045). Estos resultados preliminares muestran que los recién nacidos de madres que sufrieron dengue durante la gestación tuvieron riesgo de bajo peso al nacer y presentaron con mayor frecuencia prematurez y sufrimiento fetal, aunque se requiere aumentar el tamaño de la muestra para confirmar estos resultados. Sin embargo, es necesario estrechar la vigilancia a las madres embarazadas con dengue dados los efectos nocivos sobre la evolución del recién nacido

3D Printed hydroponic system

Hydroponic systems have been around for many years and there is a large variety of designs. This project approaches the design of a stackable hydroponic system for domestic use aiming for high yield. The 3-D printed prototype consisted of cylindrical elements each with three entries for seeding. These elements can be stacked up and rotated around one another providing configuration flexibility. In addition, the cap of our system is a half cylindrical element. A preliminary test included the use of Rockwool to grow coriander for 14 days. Being a prototype, it is still necessary to optimize the design, fine-tune its operation beyond its initial feasibility to both, maximize yield and minimize cost, which implies compromising on these two conflicting objectives.Los sistemas hidropónicos existen desde hace ya varios años en una amplia variedad de diseños y configuraciones. El proyecto que aquí se describe comprende el diseño de un sistema hidropónico modular apilable para uso doméstico, con miras a que sea uno con alta productividad. Una impresora 3D se utilizó para desarrollar el prototipo que consta de elementos cilíndricos, cada uno con tres receptáculos. A los elementos cilíndricos apilables se les puede rotar con respecto de su eje, añadiendo así mayor flexibilidad de configuración. Convenientemente, la tapa del sistema está formada por medio elemento cilíndrico. Para la prueba preliminar que aquí se describe, se depositaron semillas de cilantro por un período de 14 días en Rockwool. En su fase prototípica, se determinó la factibilidad del sistema, aunque se reconoce que es aún necesario optimizar el diseño y afinar su operación. Esto implicará conciliar dos objetivos en conflicto: maximizar la productividad del sistema y minimizar su costo total

El uso de realidad aumentada para guiar la evacuación de personas ante la ocurrencia de sismos

Augmented Reality is a promising technology to support safe pedestrian evacuation from buildings during earthquakes. The widespread availability of phone cameras makes this idea feasible. QR codes, for instance, can place virtual three-dimensional objects to guide people along a prescribed route. In this work, a prototypical system based on this premise was created in response to the 2020 sudden surge in seismic activity in Puerto Rico. The results point towards feasibility as well as to a series of areas that require improvement for a real-life implementation.La realidad aumentada es una tecnología promisoria para habilitar la evacuación segura de personas durante un sismo. La amplia disponibilidad de teléfonos móviles con cámara refuerza la factibilidad de tal idea. Es posible, por ejemplo, utilizar códigos QR para configurar objetos virtuales tridimensionales que guíen a las personas hacia una ruta prescrita. Basados en esta premisa, este trabajo describe un sistema prototípico creado en respuesta al sorpresivo aumento de actividad sísmica experimentado en Puerto Rico en el 2020. Los resultados indican su factibilidad, así como una serie de mejorías que se deben atender ante una instauración en la vida real

Thrips Microbiome Study in Commercial Avocado (<i>Persea americana</i> Mill.) from Northwest Colombian Andes (Antioquia, Colombia) Shows the Presence of <i>Wolbachia</i>, <i>Ehrlichia</i>, <i>Enterobacter</i>

Microbiota associated with insects play several important roles in their host, including protection against pathogens, provision of nutrition, and survival in hostile environments. The aim of this work was to identify the bacterial community found in avocado thrips from Northwestern Colombia (Antioquia department) in order to find isolates for potential biocontrol purposes. Culture-dependent methods based on 16S rRNA and gyrase B gene sequencing in 42 bacterial isolates allowed the identification of the genera Bacillus, Serratia, Moraxella, Pantoea, and Sphingomonas. Microbial diversity detected with the temperature gradient gel electrophoresis (TGGE) technique on three morphotypes of thrips, named brown (Scirtothrips hansoni), black (Frankliniella panamensis), and pale (Frankliniella sp.), showed a low bacterial community density (Shannon–Wiener index = 1480, p > 0.05) with significant differences among morphotypes (R = 0.7877, p = 0.0004). Results obtained with Illumina sequencing on the V1–V2 hypervariable region of the subunit 16S rRNA showed a predominant sequence in the brown morphotype (Scirtothrips hansoni) that belongs to the genus Wolbachia. The 16S amplicon analyses were extended to more samples and higher resolution using the V4–V5 hypervariable region. The results showed six additional bacteria phyla, confirming the previous observation for the dominant bacterial groups made in S. hansoni and the detection of the alternation of highly predominant genera among these thrips. Our results demonstrate that endosymbiont such as Wolbachia sp. are part of the microbiota of these pests, thereby indicating the possibility of employing this type of bacterium to improve the management of avocado thrips globally

Global variation in postoperative mortality and complications after cancer surgery: a multicentre, prospective cohort study in 82 countries

© 2021 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY-NC-ND 4.0 licenseBackground: 80% of individuals with cancer will require a surgical procedure, yet little comparative data exist on early outcomes in low-income and middle-income countries (LMICs). We compared postoperative outcomes in breast, colorectal, and gastric cancer surgery in hospitals worldwide, focusing on the effect of disease stage and complications on postoperative mortality. Methods: This was a multicentre, international prospective cohort study of consecutive adult patients undergoing surgery for primary breast, colorectal, or gastric cancer requiring a skin incision done under general or neuraxial anaesthesia. The primary outcome was death or major complication within 30 days of surgery. Multilevel logistic regression determined relationships within three-level nested models of patients within hospitals and countries. Hospital-level infrastructure effects were explored with three-way mediation analyses. This study was registered with ClinicalTrials.gov, NCT03471494. Findings: Between April 1, 2018, and Jan 31, 2019, we enrolled 15 958 patients from 428 hospitals in 82 countries (high income 9106 patients, 31 countries; upper-middle income 2721 patients, 23 countries; or lower-middle income 4131 patients, 28 countries). Patients in LMICs presented with more advanced disease compared with patients in high-income countries. 30-day mortality was higher for gastric cancer in low-income or lower-middle-income countries (adjusted odds ratio 3·72, 95% CI 1·70–8·16) and for colorectal cancer in low-income or lower-middle-income countries (4·59, 2·39–8·80) and upper-middle-income countries (2·06, 1·11–3·83). No difference in 30-day mortality was seen in breast cancer. The proportion of patients who died after a major complication was greatest in low-income or lower-middle-income countries (6·15, 3·26–11·59) and upper-middle-income countries (3·89, 2·08–7·29). Postoperative death after complications was partly explained by patient factors (60%) and partly by hospital or country (40%). The absence of consistently available postoperative care facilities was associated with seven to 10 more deaths per 100 major complications in LMICs. Cancer stage alone explained little of the early variation in mortality or postoperative complications. Interpretation: Higher levels of mortality after cancer surgery in LMICs was not fully explained by later presentation of disease. The capacity to rescue patients from surgical complications is a tangible opportunity for meaningful intervention. Early death after cancer surgery might be reduced by policies focusing on strengthening perioperative care systems to detect and intervene in common complications. Funding: National Institute for Health Research Global Health Research Unit

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

© 2022 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 licenseBackground: Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide. Methods: A multimethods analysis was performed as part of the GlobalSurg 3 study—a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital. Findings: Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3·85 [95% CI 2·58–5·75]; p<0·0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63·0% vs 82·7%; OR 0·35 [0·23–0·53]; p<0·0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer. Interpretation: Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised. Funding: National Institute for Health and Care Research