Prevalence of bacterial gastroenteritis in children attending daycare centers within Kaduna Metropolis

This research was carried out on Prevalence of bacterial gastroenteritis among children attending daycare centers within Kaduna Metropolis. Children between the age of 6-24 months were studied in Chukun Local Government, Kaduna South Local Government and Kaduna North Local Government Areas of Kaduna State, Nigeria. Two Hundred and forty (240) stool specimens were collected from eight (8) Daycare centers, where thirty samples were collected from each centre. The samples collected were cultured on Eosin Ethylene blue agar, Salmonella Shigella agar, MacConkey and Nutrient agar. Out of the 240 stools samples collected, E. coli 40%, Salmonella enteric 10%, Shigella sp 6%, proteus sp 8%, Klebsiella pneumonia 6%, Entrobacter sp 5%, Erwinina sp 3%, Citrobacter sp 7%, Yersinia sp 2%, Serriatia sp 3%, Pseudomonas aeroginosa 2%, probidencia sp 1%, Morginella morganii 1%, Alcaligenes sp 1%. The bacteria isolated were found to be sensitive to most Ciprofloxacin, Gentamycin and Ofloxacin, Pefloxacin, Chloranphenicol, tarivid, Colistin sulfate. The result obtained revealed that there could be public health breach in the maintenance and management of the Daycare centres within Kaduna Metropolis

Irradiation of Foods: A Better Alternative in Controlling Economic Losses

Food irradiation as a better alternative to other food processing methods is discussed. Irradiation is a promising new food safety technology that can eliminate disease-causing microorganisms such as E.coli 0157:H7, Camplyobacter and Salmonellae from foods; delay maturation of fruits and inhibit sprouting of bulbs and onions. The yearly and cyclic scarcity of fruits and vegetables during their non-productive period is a known phenomenon in most developing countries. Post-harvest losses are due to microorganisms, which destroy these foods. The process, consumer acceptance, nutritional and microbiological safety are highlighted in this paper. @JASE

Isolation of bacteria associated with diarrhoea among children attending some hospitals in Kano metropolis, Kano state, Nigeria

Bacteriological investigations of Diarrhoeal diseases were carried out among 300 children who were between the ages of 0 – 24 months using stool samples from five different hospitals in the metropolitan Kano. The organisms were isolated and identified using cultural, serological biochemical test. Sensitivity testing of the isolates to some antimicrobial agents was carried out. Out of those number only a total of 122(40.7%) of children were found to have Diarrhoea associated with bacteria in which 34(27.9%) and 88(72.1%) were from breast-fed and bottle-fed children respectively. The Bacteria isolated were Escherichia coli which were the most predominant, followed by Salmonella species and then Shigella species. As at the time of the study, there was significance difference between breast-fed and bottle-fed in the incidence at P> 0.05. Among the antimicrobials used cotrimoxazole was found to be more effective than the others. Also, most of the children tested were found to have a common illness of diarrhoea followed by dysentery, abdominal pain and fever/vomiting

Antibiotic susceptibilities of Salmonella species prevalent among children of 0-5 years with diarrhea in Katsina state, Nigeria

This study was conducted to assess the prevalence of Salmonella species among children having diarrhea in Katsina State, Nigeria. A total of 220 diarrhea stool samples of children aged five years and below (0-5 years) were collected and screened for Salmonella species using culture technique. Presumptively positive isolates were further screened biochemically and serologically, using MicrogenTM Enterobactericeae ID kit and MicrogenTM Salmonella rapid confirmatory latex agglutination test kit. Antibiotic susceptibility of confirmed isolates was carried out and resistance patterns of the isolates determined. The highest incidence was observed in children of 13-24 months of age and the least in children of 0-12 months of age. There was a higher prevalence in male than female children. All the isolates screened were resistant to Ampicillin and Amoxicillin and of these, 90.9% were resistant to Amoxicillin-clavulanic acid and 45.5% were resistant to Nalidixic acid. However, 100% were sensitive to Gentamicin, 90.9% were sensitive to Ciprofloxacin and Cefotaxime and 9.09% showed reduced susceptibility to Ciprofloxacin. Both the occurrence of Salmonella in children and their resistance to multiple antibiotics as observed are of public health significance. The vein of this study underscores the importance of routine monitoring of the incidence of Salmonella and continued health education of caregivers.Keywords: Antibiotic resistance; Prevalence of Salmonella; Childhood diarrhea; Katsina State; Antibiotics susceptibility of Salmonell

Mycological Quality of Powdered Herbal Medicinal Preparations Packaged for Human Consumption in North Western Nigeria

The increase in the consumption of natural drugs have made their use a public health problem due to its poor quality, presence of  fungal contamination and the risk of the presence of mycotoxins. This investigation was designed to throw light on the mycological and aflatoxigenic status of powdered herbal medicinal products marketed in North Western Nigeria. A total of four hundred and thirty two(432) powdered herbal medicinal preparations consisting of twelve(12) each from six(6) localities in each of the six(6) states’ metropolis of the North Western Nigeria were obtained. These samples were subjected to various analyses. The parameters measured were grouped as follows: level of fungal contaminations of fungi; frequency of distribution of  fungi and  mycoflora type present in the herbal preparations. Results indicated that all the four hundred and thirty two herbal medicinal preparations assessed did not comply with the maximum acceptable limit of 2x102 cfu/g for fungal load. The study showed that samples from Kaduna and Kebbi  had  a significantly  higher  mean fungal count (1.09x105cfu/g and 1.05x105cfu/g respectively) that were not significantly different (p?0.05), hence suggesting higher contamination with fungi. The least was observed in Zamfara state with the lowest mean value of fungal load. The statistical analysis showed that fungal load in Katsina, Sokoto, Kano and  Zamfara  states were  not the same but not significantly different (p?0.05). This suggested low level of contamination with fungi when compared with samples from Kaduna and Kebbi state. In terms of fungal distribution in  herbal medicinal preparations, this study indicated that  fungi of the genus Aspergillus spp and Penicillus spp were the most frequently isolated and were found to be higher in frequency of occurrence. Out of one thousand and ninety five (1095)  total frequency of occurrence of fungi in the herbal medicinal preparations, the total frequency of occurrence of Aspergillus spp in this study is seven hundred and seventy five(70.77%), Penicillium spp (n=190;17.35%); Fusarium spp (n=86;7.85%)  and Rhizopus spp (n=44;4.02%). In this finding it could be suggested that Aspergillus spp and Penicillium spp are the major contaminant of herbal drugs. In all the samples screened from the six states, higher level of contamination with Aspergillus spp were found. Among  the Aspergillus spp observed,  A. flavus, A. paraceticus, A. niger were the most frequently occurred fungi in the herbal medicinal samples suggesting that these type of fungi are the major contaminant of the herbal medicinal products in all the six states of the North West of Nigeria. The highest frequency of occurrence of fungi observed in samples from Kaduna 151(69.59%), Kano139 (72.02%),sokoto135 (73.37%), Kebbi127 (66.49%), Kastina112 (70.89%) and Zamfara111 (68.52%)  may be as a result of poor harvesting, processing and storage practice of the handler of the herbal products. The  means of  frequency of occurrence of fungal isolates in herbal preparations from the North Western Nigeria also showed that incidence of  Aspergillus flavus, Aspergillus paraceticus, Aspergillus niger and Penicillium spp were not significantly  different at p<0.05 but significantly higher than Aspergillus ochraceus and Aspergillus versicolor. The result obtained also indicates that Fusarium spp and Rhizopus spp were significantly the lowest. This result suggests that the samples of herbal medicines obtained in the North Western Nigeria is heavily contaminated with the fungal species of Aspergillus spp and Penicillium spp. Keywords:  herbal medicines, fungal contamination, North Western Nigeri

ANTIMICROBIAL SUSEPTIBILITY OF NEISSERRIA GONORRHOEAE ISOLATED FROM PATIENTS ATTENDING VARIOUS CLINICS IN PAMBEGUWA, KADUNA STATE.

Antimicrobial resistant strains of gonococcal isolates have spread in many developing countries. In most African countries, the susceptibility of Neisseria gonorrhoeae is rarely tested. The antimicrobial susceptibility of one hundred and eight (108) Neisseria gonorrhoeae was therefore determined. Of the 108 isolates tested, 80 (74.07%) were resistant to penicillin, 74 (68.5%) were resistant to ampicillin. The resistance profiles for the remaining antibiotics tested were as follows: Erythromycin (58.3%), Tetracycline (62%) and gentamicin (25.9%). All the isolates were susceptible to ceftriaxone, cefuroxime and ofloxacin. Of the 80 penicillin resistant strains, 65 (81.3%) were penicillinase producing Neisseria gonorrhoeae (PPNG). The Minimum Inhibitory Concentration (MIC) of Penicillin against 9 gonococcal isolates, Ampicillin against 5 Neisseria gonorrhoeae isolates and Tetracycline against 2 gonococcal isolates was 32.0µg/ml. A total of 108 isolates were susceptible to 0.125µg/ml of ceftriaxone, cefuroxime and ofloxacin respectively. The high prevalence of PPNG and multiple drug resistance strains of Neisseria gonorrhoeae is associated with self-medication and sexual interaction between commercial sex workers (CSWs) resident in Pambeguwa and the long-distance truck drivers. Antimicrobial susceptibility test should be carried out on every gonococcal isolate obtained to ensure effective therapy. The present study was undertaken to assess the antimicrobial susceptibility of gonococcal isolates recovered from patients in Pambeguwa,Kaduna state, Nigeria

Prevalence and Speciation of Non-albican Vulvovaginal Candidiasis in Zaria

Candida species are versatile microorganisms which live normally in the skin, mouth, gastrointestinal tract, and genitourinary tract. In healthy people, Candida species usually live as benign commensals and produce no disease. However, they are the most common cause of fungal infections in immunosuppressed individuals, leading to a range of non-life threatening mucocutaneous diseases to threatening invasive systemic diseases. Among Candida spp, the more important pathogenic species are Candida albicans, C. glabrata, C. tropicalis, C. parapsilosis and C.krusei. The research set out to determine the prevalence of non albicans vulvovaginal candidiasis and pattern of distribution of the different Candida species in Zaria.A cross sectional study of female genital swabs collected from 400 women with features suggestive of vulvovaginal candidiasis attending Gynaecology clinics in four selected hospitals in Zaria. The swabs collected between a period of February 2012 to March 2013 were analysed by microscopy and culture in the Medical Microbiology laboratory, Ahmadu Bello University, Zaria.  Data on demographic details were also obtained; using structured questionnaires. Of the 400 patient samples examined 163(40.8%) were culture positive for Candida spp. Of these 163 isolates, 84 were germ-tube negative giving a prevalence of 21% for non albicans candida (NAC). Of the 84 isolates of NAC, 51(60.7%) were Candida parapsilosis, 18 (21.4%) were Candida tropicalis, while 15 (17.9%) were Candida glabrata. Vulvovaginal candidiasis was found to be more prevalent among the age group 21-30 years in general. Vulvovaginal candidiasis is a prevalent infection among women. More than half of the infections are due to non-albicans Candida.  NAC is becoming increasingly relevant in the aetiology of Vulvovaginal candidiasis and health providers need to be aware of this as the treatment options differ. Keywords: Non albicans Candida, Vulvovaginal candidiasis, Vulvovaginiti

Functional characterization of α-Gal producing lactic acid bacteria with potential probiotic properties

The possibility of exploiting the human immune response to glycan α-Gal for the control of multiple infectious diseases has been the objective of recent investigations. In this field of research, the strain of Escherichia coli O86:B7 has been at the forefront, but this Gram-negative microorganism presents a safety concern and therefore cannot be considered as a probiotic. To address this challenge, this study explored the identification of novel lactic acid bacteria with a safe history of use, producing α-Gal and having probiotic potential. The lactic acid bacteria were isolated from different traditionally fermented foods (kununn-zaki, kindirmo, and pulque) and were screened for the production of α-Gal and some specific probiotic potential indicators. The results showed that Ten (10) out of forty (40) [25%] of the tested lactic acid bacteria (LAB) produced α-Gal and were identified as Limosilactobacillus fermentum, Levilactobacillus brevis, Agrilactobacillus composti, Lacticaseibacillus paracasei, Leuconostoc mesenteroides and Weissella confusa. Four (4) LAB strains with highest levels of α-Gal were further selected for in vivo study using a mouse model (α1,3GT KO mice) to elucidate the immunological response to α-Gal. The level of anti-α-Gal IgG observed were not significant while the level of anti-α-Gal IgM was lower in comparison to the level elicited by E. coli O86:B7. We concluded that the lactic acid bacteria in this study producing α-Gal have potential probiotic capacity and can be further explored in α-Gal-focused research for both the prevention and treatment of various infectious diseases and probiotic development.Research on α-Gal has been partially supported by Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación MCIN/AEI/10.13039/501100011033, Spain and EU-FEDER (Grant BIOGAL PID2020-116761 GB-I00). BT was supported by The World Academy of Sciences, Italy (FR number_3240306342) and the Department of Biotechnology, Government of India (BT/AB/03/04/2002Timothy).Peer reviewe

Borna disease virus (BDV) circulating immunocomplex positivity in addicted patients in the Czech Republic: a prospective cohort analysis

<p>Abstract</p> <p>Background</p> <p>Borna disease virus (BDV) is an RNA virus belonging to the family Bornaviridae. Borna disease virus is a neurotropic virus that causes changes in mood, behaviour and cognition. BDV causes persistent infection of the central nervous system. Immune changes lead to activation of infection. Alcohol and drug dependence are associated with immune impairment.</p> <p>Methods</p> <p>We examined the seropositivity of BDV circulating immunocomplexes (CIC) in patients with alcohol and drug dependence and healthy individuals (blood donors). We examined 41 addicted patients for the presence of BDV CIC in the serum by ELISA at the beginning of detoxification, and after eight weeks of abstinence. This is the first such study performed in patients with alcohol and drug dependence.</p> <p>Results</p> <p>BDV CIC positivity was detected in 36.59% of addicted patients on day 0 and in 42.86% on day 56. The control group was 37.3% positive. However, we did not detect higher BDV CIC positivity in addicted patients in comparison with blood donors (p = 0.179). The significantly higher level of BDV CIC was associated with lower levels of GGT (gamma glutamyl transferase) (p = 0.027) and approached statistical significance with the lower age of addicted patients (p = 0.064). We did not find any association between BDV CIC positivity and other anamnestic and demographic characteristics.</p> <p>Conclusions</p> <p>In our study addicted patients did not have significantly higher levels of BDV CIC than the control group. The highest levels of BDV CIC were detected in patients with lower levels of GGT and a lower age.</p> <p>Trial registration</p> <p>This study was approved by the ethical committee of the University Hospital Medical Faculty of Charles University in Pilsen, Czech Republic (registration number 303/2001).</p