Teaching Lab: Training Novice Computer Science Teachers

Student teaching assistants are not uncommon in computer science. However, their pedagogical training is often only superficial. This poster presents the Teaching Lab – a mature and fully developed training course for novice teachers (mostly undergraduate teaching assistants), its core principles, content and unique features as it evolved over five years. Our experience can be helpful to others intending to create or adjust a training program for novice teachers

Proposal for More Production Manufacturing of Lift Check Valve

Diplomová práce se zabývá problematikou výroby tělesa zpětného ventilu. Práce je zaměřena na zvýšení produktivity výrobních technologií týkajících se tohoto produktu. Zproduktivnění má dopomoci úprava či změna strojů, nástrojů a také řezných podmínek. Stroje se vybírají výhradně ze strojového parku, který má k dispozici firma Armatury KLAD spol. s r. o. Úvodní část práce je věnována představení podniku, ve kterém byla diplomová práce zpracována. Následuje popis samotného produktu, zpětného ventilu. Dále je popsána současná technologie výroby, která obsahuje jednotlivá pracoviště, stroje, nástroje a výrobní podmínky. Další část práce obsahuje návrh na zproduktivnění výroby, díky změně výrobní technologie. Zproduktivnění se dosáhne díky zkrácení výrobních časů a snížením finančních nákladů na kus. Na závěr je zmíněno technicko – hospodářské zhodnocení a porovnání stávajícího a nově navrženého výrobního postupu.Diploma thesis deals with problematic about manufacturing body of lift check valve. Thesis is focused on more productive manufacturing technologies of this product. It should be carried out by modifying or changing machines, tools and cutting conditions too. Machines are chosen only from machine park, of company Armatury KLAD spol. s r.o. Introductory part of thesis is about company, where the diploma thesis was processed. After that there is product (lift check valve) description and also description of current manufacturing procces. This category include severy single workplace, machine, tool and cutting condition. Next part is about proposal for new technology. This technology is more productive, because time for production of one piece is shorter and input costs are lower too. End of diploma thesis is about technical-economic comparison of current and newly proposed manufacturing technologies.346 - Katedra obrábění, montáže a strojírenské metrologievýborn

Proposal for More Productive Manufacturing of Sight Glass for Chemical Industry

Import 23/07/2015Bakalářská práce je zaměřena na zvýšení produktivity technologie výroby zadané součásti. Zproduktivnění se má uskutečnit vhodnou úpravou strojů, nástrojů a řezných podmínek, jež má k dispozici firma Armatury KLAD s.r.o. Úvod je věnován profilu podniku a popisu samotné součásti. Následně je popsán současný stav výroby, který obsahuje použité stroje, nástroje a stávající technologický postup, jež popisuje jednotlivé výrobní operace. Dále je uveden návrh na nový technologický postup výroby, který obsahuje volbu jiného sledu operací a nástrojů za účelem snížení výrobních časů. Závěr obsahuje porovnání nově navrhované výroby se stávající, co se týče technicko-hospodářského zhodnocení.This bachelor thesis is focused on more productive manufacturing technology of the specified component. It should be carried out by modifying machines, tools and cutting conditions, which has Armatury KLAD s.r.o. Introduction is devoted to presentation of company Armatury KLAD s.r.o and chosen component. Following is actual manufacturing process, which includes machines, tools and technological process. This technological process describes every single manufacturing operation. The following oves a proposal of a new technological process, which includes individual operation in a different order. It includes new tools, too. Aim is to reduce production times. Conclusion contains techno-economic comparison of new proposed production and actual production.346 - Katedra obrábění, montáže a strojírenské metrologievýborn

Deployment of the Context DLP System within ISMS Implementation

Diplomová práce se zabývá nasazením DLP systému do vybrané společnosti. Práce obsahuje analýzu současné situace společnosti a na základě zjištění poskytuje rozhodnutí pro výběr nejvhodnějšího DLP řešení. Nakonec popisuje reálné nasazení vybraného DLP systému do společnosti.This diploma thesis focuses on a DLP implementation within a specific organization. The thesis contains current situation analysis and provides decision for choice of the most suitable DLP based on the analysis findings. Eventually describes a real implementation of the chosen DLP system within the organization.

Circulating tumor cells in urological cancers

Circulating tumor cells (CTC) represent a very small subpopulation of the cancer cells found in the bloodstream of patients in the metastatic phase of neoplastic disease. Due to the timeline of the disease, they are regarded as a negative prognostic marker. This study focused on determining CTC percentages; these values vary be­tween different types of cancer. In addition to their diagnostic use, CTCs may also be used to treat the disease. Calculating CTC population size and analyzing their biology in patients in advanced stages of cancer may prove valuable in creating a molecular profile for the disease. This would strongly encourage diagnostics and enable personalized treatment. We here present an analysis of recent data on CTCs in urological cancers and their potential uses

Circulating tumor cells in urological cancers

Circulating tumor cells (CTC) represent a very small subpopulation of the cancer cells found in the bloodstream of patients in the metastatic phase of neoplastic disease. Due to the timeline of the disease, they are regarded as a negative prognostic marker. This study focused on determining CTC percentages; these values vary between different types of cancer. In addition to their diagnostic use, CTCs may also be used to treat the disease. Calculating CTC population size and analyzing their biology in patients in advanced stages of cancer may prove valuable in creating a molecular profile for the disease. This would strongly encourage diagnostics and enable personalized treatment. We here present an analysis of recent data on CTCs in urological cancers and their potential uses. (Folia Histochemica et Cytobiologica 2017, Vol. 55, No. 3, 107–113

Cardiolipin-containing lipid membranes attract the bacterial cell division protein diviva

DivIVA is a protein initially identified as a spatial regulator of cell division in the model organism Bacillus subtilis, but its homologues are present in many other Gram-positive bacteria, including Clostridia species. Besides its role as topological regulator of the Min system during bacterial cell division, DivIVA is involved in chromosome segregation during sporulation, genetic competence, and cell wall synthesis. DivIVA localizes to regions of high membrane curvature, such as the cell poles and cell division site, where it recruits distinct binding partners. Previously, it was suggested that negative curvature sensing is the main mechanism by which DivIVA binds to these specific regions. Here, we show that Clostridioides difficile DivIVA binds preferably to membranes containing negatively charged phospholipids, especially cardiolipin. Strikingly, we observed that upon binding, DivIVA modifies the lipid distribution and induces changes to lipid bilayers containing cardiolipin. Our observations indicate that DivIVA might play a more complex and so far unknown active role during the formation of the cell division septal membrane

The analysis of serum mineral profile of cows before and after calving: A case study

Article Details: Received: 2020-10-14 | Accepted: 2020-11-27 | Available online: 2021-01-31https://doi.org/10.15414/afz.2021.24.mi-prap.89-92High-yielding dairy cows have several critical periods during lactation. These include the drying period, parturition, postpartum period and the first 100 days of lactation. The mineral profile displays the balanced feed ration appropriate for the category. Therefore, investigation of serum mineral profile of dry cows and cows in 12th week after calving was the aim of this study. In this work 12 high producing Holstein Friesian dairy cows from dairy farm were used. The analysed elements Ca2+, P, Na+, K+, Mg2+ and chlorides (Cl-) were determined at 4th week after drying  and during 12th week after calving. The data obtained were compared with the standard for dairy cows. Compared to reference interval, before calving 8 and after calving 5 cows had hypocalcaemia. Hypophosphatemia was detected for 2 cows before calving and 3 after calving. For the other elements Na, K, Mg2+ and chlorides (Cl-) no deviations from the reference intervals were found. In blood serum mineral profile between dry cows and cows in 12th week of lactation significant differences were found for Mg Cl- (increase) and K (decrease). Results shown, that main problem of dry and lactating cows was the hypocalcaemia and partly the hypophosphatemia.Keywords: metabolic test, mineral profile, blood serum, dairy cowReferencesAiello, S. E. (1998). The Merck veterinary manual (No. Ea9 DEPOSITO FV).Bertoni, G., Trevisi, E., & Lombardelli, R. (2009). Some new aspects of nutrition, health conditions and fertility of intensively reared dairy cows. Italian Journal of Animal Science, 8(4), 491-518. 10.4081/ijas.2009.491Blood, D.C., &Radostits, O.M. (1994). Veterinary Medicine: A textbook of the diseases of cattle, sheep, pigs, goats and horses. 8th ed. W.B. Saunders Co.Burke, C. R., Meier, S., McDougall, S., Compton, C., Mitchell, M., & Roche, J. R. (2010). Relationships between endometritis and metabolic state during the transition period in pasture-grazed dairy cows. Journal of dairy science, 93(11), 5363-5373. 10.3168/jds.2010-3356 Donat, K., Siebert, W., Menzer, E., & Söllner-Donat, S. (2016). Long-term trends in the metabolic profile test results in German Holstein dairy herds in Thuringia, Germany. Tierärztliche Praxis Ausgabe G: Großtiere/Nutztiere, 44(02), 73-82.  10.15653/TPG-150948Gross, J., van Dorland, H. A., Bruckmaier, R. M., & Schwarz, F. J. (2011). Performance and metabolic profile of dairy cows during a lactational and deliberately induced negative energy balance with subsequent realimentation. Journal of dairy science, 94(4), 1820-1830. https://doi.org/10.3168/jds.2010-3707Hadžimusić, N., & Krnić, J. (2012). Values of calcium, phosphorus and magnesium concentrations in blood plasma of cows in dependence on the reproductive cycle and season. Journal of Faculty of Veterinary Medicine, Istambul University, 38(1), 1-8.Hanušovský, O., Šimko, M., & Bíro, D. (2017) Kontinuálne sledovanie parametrov bachorového prostredia využitím prenosu dát nízkofrekvenčným signálom. Nitra: Slovenská poľnohospodárska univerzita v Nitre, ISBN 978-80-552-1746-8.Hofírek, B., Dvořák, R., Němeček, L., Doležel, R., & Pospíšil, Z. (2009). Nemoci skotu. 1. vyd., Brno: Noviko as, Česká buiatrická společnost, 1149 s.Jovanovic, J.M., Rajic, I., Pesterac, V., Crcev, D., & Cokrevski, S. (1997). Parametri krvi visoko steonih i tek oteljenih krava hranjenih obrocima razlicitog sastava. Veterinarski Glasnik 51, 231-244.Kahn, C. M. (2005). The Merck Veterinary Manual 9th ed. White house station, NJ, USA, Merck & CO. Kaneko, J.J. (2008). Carbohydrate Metabolism and Its Diseases. In: Kaneko, J.J., Harvey, J.W., Bruss, M.L., (Eds.), Clinical biochemistry of domestic animals. 6th ed. New York, Academic Press. https://doi.org/10.1016/B978-012396305-5/50004-XKantíková, M., & Balážik, T. (2003). Diagnostika metabolických porúch alebo prevencia. Slovenský chov, 8(7), 39-40.Kraft, W. (Ed.). (2005). Klinische labordiagnostik in der tiermedizin. Schattauer Verlag.LeBlanc, S. J., Leslie, K. E., & Duffield, T. F. (2005). Metabolic predictors of displaced abomasum in dairy cattle. Journal of dairy science, 88(1), 159-170. https://doi.org/10.3168/jds.S0022-0302(05)72674-6Palmer, L. S., & Eckles, C. H. (1927). Effect of Phosphorus Deficient Rations on Blood Composition in Cattle. Proceedings of the Society for Experimental Biology and Medicine, 24(4), 307-309. https://doi.org/10.3181/00379727-24-3341Prodanović, R., Kirovski, D., Jakić-Dimić, D., Vujanac, I., & Kureljušić, B. (2010). Telesna kondicija i pokazatelji energetskog statusa krava u visokom graviditetu i ranoj fazi laktacije. Veterinarski glasnik, 64(1-2), 43-52. 10.2298/VETGL1002043PSchröder, B., & Breves, G. (2006). Mechanisms and regulation of calcium absorption from the gastrointestinal tract in pigs and ruminants: comparative aspects with special emphasis on hypocalcemia in dairy cows. Animal Health Research Reviews, 7(1-2), 31. 10.1017/S1466252307001144 Šamanc, H., Stojić, V., Kirovski, D., Jovanović, M., Cernescu, H., & Vujanac, I. (2010). Thyroid hormones concentrations during the mid-dry period: An early indicator of fatty liver in Holstein-Friesian dairy cows. Journal of thyroid research, 2010. https://doi.org/10.4061/2010/897602Šimko, M., Bíro, D., Čerešňáková, Z., Juráček, M., & Gálik, B. (2009). The effect of wheat and maize meal on rumen fermentation and apparent nutrient digestibility in cattle. Slovak Journal of Animal Science, 42(Supplement), 99-103.Slanina, Ľ., & Sokol, J. (1991). Vademecum veterinárneho lekára. Bratislava, Príroda

Analysis of movement mechanics in the training process of sport horses

Article Details: Received: 2020-10-15 | Accepted: 2020-11-27 | Available online: 2021-01-31https://doi.org/10.15414/afz.2021.24.mi-prap.145-149The aim of the study was to determine the effect of training process on changes of quantitative and qualitative indicators of the movement mechanics of sport horses. A load controller was used to evaluate the motion mechanics. The training load was divided into three stages with different lengths, speeds and degrees of load. Tested animals were 14 horses of Slovak Warmblood breed. Our results have shown that gradual increase of load in the tested horses resulted in better spaciousness of movement, which was reflected in the elongation of the horse´s stride, regular movement and significant energy of the pelvic limbs (p<0.05). From our findings we can state that the effect of gender did not have significant effect on the number and length of steps in each degree of load. We have noticed a significant difference (p<0.05) in the „number of steps“ parameter between the age categories of horses in the age up to 7 years (3.460±619) and the category aged up to 14 years (3.383±572). Gradual increase of the training load led to the economization of the movement and the long-term training process sled to the adaptation of the organism to repeated load. Keywords: horse, movement mechanics, training, treadmill, loadReferencesArfuso, F. et al. (2016). Dynamic modulation of platelet aggregation, albumin and nonesterified fatty acids during physical exercise in Thoroughbred horses. Research in Veterinary Science, 104, 86-91.https://doi.org/10.1016/j.rvsc.2015.11.013Barrey, E. (2016). Biomechanics of locomotion in the athletic horses. Veterian Key [online], 10. Retrieved June 20, 2019 from https://veteriankey.com/biomechanics-of-locomotion-in-the-athletic-horse/Barrey, E. et al. (1993). Stride characteristics of overground versus treadmill locomotion in the saddle horse. Acta Anatomica,146(2-3), 90-94. https://doi.org/10.1159/000147427Becero, M. et al. (2020). Capacitive resistive electric transfer modifies gait pattern in horses exercised of treadmill. BMC Veterinary Research, 16, 10. https://doi.org/10.1186/s12917-020-2233-x  Becker, A. C., Stock, K. F. & Distl, O. (2011). Genetic correlations between free movement and movement under rider in performance tests of German Warmblood horses. Livestock Science, 142(1-3), 245-252.https://doi.org/10.1016/j.livsci.2011.08.001Clayton, H. M. (2004). The dynamic horse: a biomechanical guide to equine movement and performance. Madison, MI: Sport Horse Publications. ISBN 097476700X.Fredricson, I. et al. (1983). Treadmill for equine locomotion analysis. Equine Veterinary Journal, 15(2), 111-115. https://doi.org/10.1111/j.2042-3306.1983.tb01730.xHalo, M. et al. (2008). Genetic efficiency parameters of Slovak warm-blood horses. Arch. Tierz., Dummerstorf 51 (2008) 1, 05-15.Halo, M. et al. (2008). Influence stres on the training process of the horses. Journal of Central European Agriculture Open Access, 9(1), 217-223.Leleu, C., Cotrel, C. & Barrey, E. (2005). Relationships between biomechanical variables and race performance in French Standardbred trotters. Livestock Production Science, 92(1), 39-46.https://doi.org/10.1016/j.livprodsci.2004.07.019  Mcbride, S. D. & Mills, D. S. (2012). Psychological factors affecting equine performance. BMC Veterinary Research, 8, 180. https://doi.org/10.1186/1746-6148-8-180McGreevy, P. D. & McLean, A. N. (2007). Roles of learning theory and ethology in equitation. Journal of Veterinary Behavior: Clinical Applications and Research, 2(4), 108-118. https://doi.org/10.1016/j.jveb.2007.05.003Mlyneková, E. et al (2016). Impact of training load on the heart rate of horses. Acta fytotechnica et zootechnica, 19, 2016(4):167-170. http://dx.doi.org/10.15414/afz.2016.19.04.167-170Moore, J. (2010). General biomechanics: the horse as a biological machine. Journal of Equine Veterinary Science, 30(7), 379-383. https://doi.org/10.1016/j.jevs.2010.06.002Parkes, R. S. V. et al. (2019). The Effect of Training on Stride Duration in a Cohors of Two-Year-Old and Three-Year-Old Thoroughbred Racehorses. Animals, 9(7), 466. https://doi.org/10.3390/ani9070466Persson, S. G. P. (1967). On blood volume and working capacity in horses. Acta Veterinaria Scandinavica, 19(Suppl.), 9-189.Straub, R. & Hoppeler, U. (1989). Leistungstest im Feld und auf dem Laufband - Eine vergleichende Studie.  2nd Congress of the world Equine Vet. Assoc. Essen: Equitana

Non–T Cell Activation Linker (NTAL): A Transmembrane Adaptor Protein Involved in Immunoreceptor Signaling

A key molecule necessary for activation of T lymphocytes through their antigen-specific T cell receptor (TCR) is the transmembrane adaptor protein LAT (linker for activation of T cells). Upon TCR engagement, LAT becomes rapidly tyrosine phosphorylated and then serves as a scaffold organizing a multicomponent complex that is indispensable for induction of further downstream steps of the signaling cascade. Here we describe the identification and preliminary characterization of a novel transmembrane adaptor protein that is structurally and evolutionarily related to LAT and is expressed in B lymphocytes, natural killer (NK) cells, monocytes, and mast cells but not in resting T lymphocytes. This novel transmembrane adaptor protein, termed NTAL (non–T cell activation linker) is the product of a previously identified WBSCR5 gene of so far unknown function. NTAL becomes rapidly tyrosine-phosphorylated upon cross-linking of the B cell receptor (BCR) or of high-affinity Fcγ- and Fcɛ-receptors of myeloid cells and then associates with the cytoplasmic signaling molecules Grb2, Sos1, Gab1, and c-Cbl. NTAL expressed in the LAT-deficient T cell line J.CaM2.5 becomes tyrosine phosphorylated and rescues activation of Erk1/2 and minimal transient elevation of cytoplasmic calcium level upon TCR/CD3 cross-linking. Thus, NTAL appears to be a structural and possibly also functional homologue of LAT in non–T cells