The genus Ruteria roudier, 1954 (Curculionidae) in Serbia

The presence of Ruteria graeca (Caldara, 1973) and R. hypocrita (Boheman, 1837) (93 and 215 specimens respectively) was confirmed among other adult soil weevil material collected at 24 localities on the territory of Serbia between 1995 and 2003 for the most part using pitfall traps. Ruteria hypocrita was much more frequent. In both species, males were dominant (36.55 and 37.21%, respectively). Biogeographically, the new Ruteria graeca findings are an impor- tant supplement completing the picture of the mosaic distribution of this species, endemic to the Balkan Peninsula. Until now, the given species was completely unknown in Serbia, i.e., in the central part of the Balkan Peninsula. Our data show a new northern boundary of its distribution. In addition, we provide ecological details about the finding places of both species. Briefly, different deciduous and mixed deciduous-coniferous woods at various altitudes and on different geological substrates are host ecosystems for Ruteria

On the Topological Resonance Energy of Porphins and Related Structures

Porphinoid, both existing and hypothetical systems, are represented by graphs at the nearest-neighbour level of approximation. By dimer covering of these graphs characteristic and reference polynomials are obtained and the topological resonance energy is calculated. It has been found that aromatic stabilization of porphins is mainly due to the high topological resonance energy value of pyrrole-type rings, while aromatic stabilization of phthalocyanins is due mainly to high resonance stabilization of i-indole-type rings

On the Topological Resonance Energy of Coumarin and Its Derivates

Topological resonance energies of coumarin and the following derivatives: 3-hydroxycoumarin, 4-hydroxycoumarin, 3,4-dihydro-. xycoumarin, 4,6-dihydroxycoumarin, 4,5,7-trihydroxycoumarin, 3- -carboxy-4-hydroxycoumarin, 3-bromo-4-hydroxycoumarin, and 6-bromo-4-hydroxycoumarin, are reported. Theoretical predictions that these all coumarins should exhibit aromatic properties are sustained by the ample experimental evidence. In addition, coumarin and isocoumarin are predicted to be much more stable than the corresponding quinonoid isomer

On the Topological Resonance Energy of Coumarin and Its Derivates

Topological resonance energies of coumarin and the following derivatives: 3-hydroxycoumarin, 4-hydroxycoumarin, 3,4-dihydro-. xycoumarin, 4,6-dihydroxycoumarin, 4,5,7-trihydroxycoumarin, 3- -carboxy-4-hydroxycoumarin, 3-bromo-4-hydroxycoumarin, and 6-bromo-4-hydroxycoumarin, are reported. Theoretical predictions that these all coumarins should exhibit aromatic properties are sustained by the ample experimental evidence. In addition, coumarin and isocoumarin are predicted to be much more stable than the corresponding quinonoid isomer

Pancreatic cancer mortality in Serbia from 1991-2010-a joinpoint analysis

Aim To analyze the trends of pancreatic cancer mortality in Serbia. Methods The study covered the population of Serbia in the period 1991 to 2010. Mortality trends were assessed by the joinpoint regression analysis by age and sex. Results Age-standardized mortality rates ranged from 5.93 to 8.57 per 100 000 in men and from 3.51 to 5.79 per 100 000 in women. Pancreatic cancer mortality in all age groups was higher among men than among women. It was continuously increasing since 1991 by 1.6% (95% confidence interval [CI] 1.1 to 2.0) yearly in men and by 2.2% (95% CI 1.7 to 2.7) yearly in women. Changes in mortality were not significant in younger age groups for both sexes. In older men (≥55 years), mortality was increasing, although in age groups 70-74 and 80-84 the increase was not significant. In 65-69 years old men, the increase in mortality was significant only in the period 2004 to 2010. In≥50 years old women, mortality significantly increased from 1991 onward. In 75-79 years old women, a non-significant decrease in the period 1991 to 2000 was followed by a significant increase from 2000 to 2010. Conclusion Serbia is one of the countries with the highest pancreatic cancer mortality in the world, with increasing mortality trend in both sexes and in most age groups

Istraživanje mehanizma i kinetika procesa oksidacije halkopiritnog koncentrata

The oxidation process of chalcopyrite concentrate ″Rudnik″ (Serbia) was investigated by DTA-TG measurements under air conditions in the temperature range of 298 – 1173 K, as well as by XRD analysis of the oxidation products at 723, 873 and 1173 K. Based on the results obtained, the mechanism of chalcopyrite concentrate oxidation process was given, contributing to the understanding of the successive chemical reactions accompanying the formation of intermediate products. The kinetic study of the investigated process was done according to the methods of Kissinger and Ozawa, which enabled determination of the activation energy values of the chemical reactions proceeding during the chacopyrite concentrate oxidation.Proces oksidacije halkopiritnog koncentrata ″Rudnik″ (Srbija) ispitivan je DTA-TG mjerenjima u atmosferi zraka i temperaturnom intervalu 298–1173 K, kao i XRD analizama produkata oksidacije na 723, 873 i 1173 K. Na temelju dobivenih rezultata predložen je mehanizam oksidacije halkopiritnog koncentrata uključujući i kemijske reakcije koje prate stvaranje odgovarajućih međuproizvoda. Za ispitivanje kinetike procesa rabljene su metode Kissinger i Ozawa, kojima su određene vrijednosti energija aktivacije kemijskih reakcija tokom oksidacije halkopiritnog koncentrata

Identification and phylogenetic analysis of norovirus detected in mussels (Mytilus galloprovincialis) from the aspect of food safety

Virusi koji se prenose hranom predstavljaju sve veći problem sa aspekta bezbednosti hrane i javnog zdravlja ljudi. Norovirus je jedan od vodećih uzročnika epidemijskih i sporadičnih slučajeva gastroenteritisa u svetu. Imajući u vidu da ne postoje relevantni podataci o prevalenci ovog virusa u dagnjama dostupnih na trţištu u našoj zemlji, koje dolaze iz crnogorskog akvatorijuma i da je metoda za njihovu detekciju na samom početku razvoja, cilj ove doktorske disertacije je da se primenom molekularno-bioloških tehnika utvrdi prisustvo, da se izvrši identifikacija i genotipizacija norovirusa u dagnjama (Mytilus galloprovincialis), ispita korelacija sa brojem E.coli u dagnjama i brojem mikroorganizama u morskoj vodi i proveri uticaj fizičko-hemijskih i bioloških parametara na pojavu norovirusa. Ukupno su ispitana 72 kompozitna uzorka, dobijena od oko 1000 komada dagnji. Ispitivanje je raĎeno na dagnjama sakupljanim u jednogodišnjem periodu (od jula 2015. do jula 2016. god.) sa 6 različitih lokacija iz registrovanih uzgajališta u Crnoj Gori. Uzorci su ispitivani metodom Real Time RT-PCR na prisustvo GI i GII genogrupe norovirusa. Efikasnost ekstrakcije svih uzoraka bila je zadovoljavajuća. Norovirus je utvrĎen u 31 uzorku (43%), sa znatno većim brojem GII pozitivnih uzoraka (54,8%) nego GI (12,9 %), dok je kod 10 (32,3%) uzoraka utvrĎeno istovremeno prisustvo norovirusa obe genogrupe (GI+GII). Metodom genotipizacije utvrĎeno je da su svi detektovani norovirusi genogrupe GI pripadali genotipu GI.2, dok je većina detektovanih norovirusa genogrupe GII pripadala genotipu GII.4, izuzev jednog koji je pripadao genotipu GII.2. Rezultati filogenetske analize ukazuju na genetsku sličnost izmeĎu norovirusa izolovanih u okviru ove disertacije i onih ranije opisanih u svetu. Na osnovu nukleotidne sekvence ispitanog ORF2 fragmenta sojeva NOV_GI_MNE 1, 2, 3, 4 utvrĎena je sličnost od 98,1% do 99,2% sa izolatom Southampton virusa (L07418), a za nukleotidne sekvence ispitanog ORF2 fragmenta sojeva NOV_GII_MNE 2, 3, 6, 7, 8, 9, 10, 11, 14, 17, 22, 24 utvrĎena je sličnost od 96,4% do 98,5% sa izolatom Hu/GII.4/sydney/NSW05 (JX459908), trenutno najrasprostranjenijem cirkulirajućem epidemijskim sojem...In terms of food safety and public health, foodborne viruses represent a increasing problem. Norovirus is one of the leading causes of epidemic and sporadic cases of gastroenteritis worldwide. Bearing in mind that there are no relevant data on the prevalence of this virus in mussels, available on the market in our country that come from the Montenegrin aquatorium and that the method for their detection at the very beginning of development. Therefore, the aim of this dissertation was to determine presence, to identify and genotype norovirus in mussels (Mytilus provincialis), to examine the correlation between the presence of the virus and the amount of E. coli in mussels; and to determine the influence of physico-chemical factors on virus infections by molecular genetic techniques. Seventy two samples, obtained from about 1000 mussels, were analyzed with satisfactory efficiency of the extraction. The examination was performed on the mussels collected in a one-year period (between July 2015 and July 2016), on six different locations. The samples were examined for the presence of GI and GII virus genotypes, using Real Time RT-PCR method. Norovirus was found in 31 samples (43%) with significantly higher number of GII positive samples (54,8%) than GI positive samples (12,9%). The presence of norovirus of both genogroups (GI + GII) was determined in 10 (32.3%) of the samples. Analyzes of the genotypes showed that all GI positive samples ascribed to genotypes GI.2, 12 GII positive samples ascribed to genotypes GII.4, while 1 GII sample ascribed to genotypes GII.2. The results of phylogenetic analysis showed the genetic link between our isolates and those previously described in other studies. Genotyping was based on the partial ORF2 sequences: NOV_GI_MNE 1, 2, 3, 4 we found 98,1% and 99,2% similarity with Southampton virus (L07418), and for ORF2 sequences NOV_GII_MNE 2, 3, 6, 7, 8, 9, 10, 11, 14, 17, 22, 24 we found 96,4% do 98,5% similarity with Hu/GII.4/sydney/NSW05 (JX459908)..

Revealing the nature of central emission nebulae in the dwarf galaxy NGC 185

In this paper we present new optical observations of the galaxy NGC 185 intended to reveal the status of supernova remnants (SNRs) in this dwarf companion of the Andromeda galaxy. Previously, it was reported that this galaxy hosts one SNR. Our deep photometric study with the 2m telescope at Rozhen National Astronomical Observatory using narrow-band H$\alpha$ and [SII] filters revealed complex structure of the interstellar medium in the center of the galaxy. To confirm the classification and to study the kinematics of the detected nebulae, we carried out spectroscopic observations using the SCORPIO multi-mode spectrograph at the 6m telescope at the Special Astrophysical Observatory of the Russian Academy of Science, both in low- and high-resolution modes. We also searched the archival X-ray and radio data for counterparts of the candidate SNRs identified by our optical observations. Our observations imply the presence of one more SNR, one possible HII region previously cataloged as part of an SNR, and the presence of an additional source of shock ionization in one low-brightness PN. We detected enhanced [SII]/H_alpha and [NII]/H_alpha line ratios, as well as relatively high (up to 90 km s$^{-1}$) expansion velocities of the two observed nebulae, motivating their classification as SNRs (with diameters of 45 pc and 50 pc), confirmed by both photometric and spectral observations. The estimated electron density of emission nebulae is 30 - 200 cm$^{-3}$. Archival XMM-Newton observations indicate the presence of an extended, low-brightness, soft source in projection of one of the optical SNRs, whereas the archival VLA radio image shows weak, unresolved emission in the center of NGC 185.Comment: 15 pages, 14 figures, accepted for publication in A&

Modulacija eksperimentalnog autoimunog encefalomijelitisa (EAE) DA pacova levamizolom

We investigated the influence of an antiparasitic drug, levamisole (2,3,5,6 - tetrahydro - 6- phenyl-imidazo (2,1 - b) thiazole -hydrochloride) with potent immunomodulatory properties on the course and development of experimental autoimmune encepha-lomyelitis (EAE). EAE was induced in female Dark Agouti (DA) rats aged two months by immunization with guinea pig spinal cord in complete Freunds adjuvant. Following immunization animals were subcutaneously treated every other day with 2.2 mg/kg levamisole. The course, development and characteristics of this autoimmune process were monitored as indirect indicators of immune system activity. Our results indicate that in EAE levamisole exerts immunosuppressive effects when administered every other day from the moment of immunization until the end of the disease. This application regime and dose postponed the onset of the first clinical signs, shortened the duration of the disease, abrogated the severity of clinical symptoms and accelerated the recovery of sick animals. In the period of induction and during EAE, levamisole also decreased the severity of changes in the cerebral perivascular spaces. In the peripheral blood of levamisole treated animals with induced EAE, a significant increase of CD4-CD8+ T cells was demonstrated. Furthermore, all rats with induced EAE had decreased numbers of CD4+CD8- T cells in their blood. These changes were in correlation with clinical signs of EAE.U ovom radu su prikazani rezultati ispitivanja anthelmintika levamizola (2,3,5,6 tetrahidro - 6 - fenil - imidazo (2,1 - b) tiazol hidrohlorida) sa snažnim imunomodulatornim svojstvima na tok i razvoj eksperimentalnog autoimunog encefalomijelitisa (EAE). EAE je indukovan imunizacijom ženki pacova soja DA (Dark Agouti) starih dva meseca pomoću homogenata kičmene moždine zamorčeta u kompletnom Freundovom adjuvansu. Posle imunizacije, životinje su tretirane subkutanim injekcijama levamizola (2.2 mg/kg) svaki drugi dan a praćeni su tok, razvoj i karakteristike ovog autoimunog oboljenja kao indirektni indikatori aktivnosti imunološkog sistema. Postignuti rezultati ukazuju dalevamizol ispoljava imunosupresivno delovanje u modelu EAE ako se aplikuje svaki drugi dan od momenta imunizacije do kraja bolesti. Primenjena doza i režim aplikacije odložili su momenat pojavljivanja prvih kliničkih simptoma, skratili trajanje bolesti, ublažili ispoljavanje simptoma i ubrzali oporavak bolesnih životinja. U periodu indukcije i tokom EAE-a levamizol je smanjio stepen promena u cerebralnim perivaskularnim prostorima. U ženskoj krvi ženki pacova sa indukovanim EAE i tretiranim levamizolom uočeno je značajno povećanje broja CD4-CD8+ T ćelija. Osim toga, u obe imunizovane grupe životinja zapaženo je smanjenje broja CD4+CD8- ćelija. Ove promene su bile u skladu sa kliničkom slikom bolesti