<i>Macrolepiota procera</i> (Scop.) Singer. Mantarının Ağır Metal İçeriklerinin ve Oksidatif Stres Durumunun Belirlenmesi

İnsanlar yüzyıllardır mantarları doğrudan doğal ortamlarından elde etme yoluna gitmiş ve farklı amaçlarla kullanmıştır. İnsanların doğadan temin ettikleri mantarların faydalarının ve zararlarının tespiti insan sağlığı açısından önemlidir. Bu nedenle bu çalışmada, halk arasında şemsiye mantarı olarak bilinen ve yaygın olarak tüketilen Macrolepiota procera (Scop.) Singer mantarının Balıkesir (Kazdağı Milli Parkı) ve Antalya (Akseki) illerinden toplanan örneklerinin TAS (Toplam Antioksidan Seviyesi), TOS (Toplam Oksidan Seviyesi) seviyeleri ile mantar ve toprak örneklerinin ağır metal içeriklerinin belirlenmesi ve kıyaslanması amaçlanmaktadır.  Yapılan çalışmalar sonucunda Akseki’den toplanan mantar örneklerinde OSİ (TOS/TASx10) değerinin 0.367, Kazdağı’ndan toplanan örneklerde ise 0.235 olarak belirlenmiştir.  Ağır metal seviyelerinin Akseki’den toplanan örneklerde daha fazla olduğu tespit edilmiştir.  Kazdağı Milli Parkından toplanan örneklerin daha sağlıklı olduğu görülmektedir. Ayrıca Kazdağı’nda mantarların yetişmesi için daha az oksidatif stresin olduğu ve buna bağlı olarak daha uygun bir ortam olduğu söylenebilir

Melatonin Modulates NMDA-Receptor 2B/Calpain-1/ Caspase-12 Pathways in Rat Brain After Long Time Exposure to GSM Radiation.

To investigate the potential protective effects of melatonin on the chronic radiation emitted by third generation mobile phones on the brain

Melatonin Modulates NMDA-Receptor 2B/Calpain-1/Caspase-12 Pathways in Rat Brain After Long Time Exposure to GSM Radiation

AIM: To investigate the potential protective effects of melatonin on the chronic radiation emitted by third generation mobile phones on the brain. MATERIAL and METHODS: A total of 24 male Wistar albino rats were divided into four equal groups. Throughout a 90-day experiment, no application was performed on the control group. The second group was exposed to 2100 MHz radiation for 30 minutes. Subcutaneous melatonin was injected into the third group. Subcutaneous melatonin injection was applied 40 minutes before radiation and then the fourth group was exposed to radiation for 30 minutes. At the end of the experiment, brain (cerebrum and cerebellum) tissues were taken from the subjects. Histochemical, immunohistochemical, ultrastructural and western blot analyses were applied. In addition to brain weight, Purkinje cells' number, immunohistochemical H Score analyses and the results of the Western blot were examined statistically. RESULTS: With the application of radiation, neuronal edema, relatively-decreased numbers of neurons on hippocampal CA1 and CA3 regions, displacement of the Purkinje neurons and dark neurons findings were observed as a result of histochemical stainings. Radiation also activated the NMDA-receptor 2B/Calpain-1/Caspase-12 pathway, NMDA-receptor 2B and Calpain-1 with the findings being supported by western blot analyses. Pre-increased protein synthesis before apoptosis was identified by electron microscopy. CONCLUSION: Mobile phone radiation caused certain (ultra) structural changes on the brain and activated the NMDA-receptor 2B/Calpain-1/Caspase-12 pathway; in addition, melatonin was found to be effective, but insufficient in demonstrating the protective effects