The Reverse Transcriptase Encoded by LINE-1 Retrotransposons in the Genesis, Progression, and Therapy of Cancer

In higher eukaryotic genomes, Long Interspersed Nuclear Element 1 (LINE-1) retrotransposons represent a large family of repeated genomic elements. They transpose using a reverse transcriptase (RT), which they encode as part of the ORF2p product. RT inhibition in cancer cells, either via RNA interference-dependent silencing of active LINE-1 elements, or using RT inhibitory drugs, reduces cancer cell proliferation, promotes their differentiation and antagonizes tumor progression in animal models. Indeed, the nonnucleoside RT inhibitor efavirenz has recently been tested in a phase II clinical trial with metastatic prostate cancer patients. An in-depth analysis of ORF2p in a mouse model of breast cancer showed ORF2p to be precociously expressed in precancerous lesions and highly abundant in advanced cancer stages, while being barely detectable in normal breast tissue, providing a rationale for the finding that RT-expressing tumours are therapeutically sensitive to RT inhibitors. We summarise mechanistic and gene profiling studies indicating that highly abundant LINE-1-derived RT can sequester RNA substrates for reverse transcription in tumor cells, entailing the formation of RNA:DNA hybrid molecules and impairing the overall production of regulatory miRNAs, with a global impact on the cell transcriptome. Based on these data, LINE-1-ORF2 encoded RT has a tumor-promoting potential that is exerted at an epigenetic level. We propose a model whereby LINE1-RT drives a previously unrecognized global regulatory process, the deregulation of which drives cell transformation and tumorigenesis and possibly implicated in cancer cell heterogeneity

Renovation of a social house into a NZEB: Use of renewable energy sources and economic implications

The EU Member States are being involved to develop long-term strategies and to promote investments aimed at improving the energy efficiency of the building stock, at increasing the use of Renewable Energy Sources (RES) and at growing the number of Nearly Zero-Energy Buildings (NZEBs). The aim of this article is to investigate energy and economic implications related to the exploitation of RES in the transformation of an Italian social housing building-type into a NZEB. The research is based on a detailed energy audit procedure that includes cost-optimal assessment and compliance check with the legal requirements. A parametric analysis was performed to find out the technical building system configurations that verify the minimum share of RES established by the Italian regulations, and at the same time to assess global cost and payback period. The intersection between legal compliancy and costeffectiveness narrows the field of applicable RES technologies that are limited to electric heat pump for heating and cooling coupled with PV system, and low size solar collectors coupled with low temperature generator for domestic hot water. Improvements in the energy policy are necessary to guarantee the best trade-off between RES exploitation, energy efficiency and costs, as to preserve market equilibrium

Climate change impact on the future performance of Nearly Zero Energy Buildings : a case study base analysis

Considering buildings large share of Europe's final energy consumption and CO2 emissions, the concept of nearly zero-energy building (NZEB) has received considerable attention. However, the changes in the performance of an NZEB due to climate change has not been studied sufficiently. This paper investigates the effects of climate change on the energy performance of NZEBs in different climatic zones in Italy, for the mid-term (2050s) and the long-term (2080s) periods. The results indicate that climate change affects the energy balance of the NZEBs, while the extent varies among different climatic regions and time periods

Geometrical bounds on irreversibility in open quantum systems

Clausius inequality has deep implications for reversibility and the arrow of time. Quantum theory is able to extend this result for closed systems by inspecting the trajectory of the density matrix on its manifold. Here we show that this approach can provide an upper and lower bound to the irreversible entropy production for open quantum systems as well. These provide insights on the thermodynamics of the information erasure. Limits of the applicability of our bounds are discussed, and demonstrated in a quantum photonic simulator

Fostering Sustainability @UniTs

Promoting sustainability through innovative culture and models is nowadays a top task for universities. Their commitment oriented by the 17 United Nations’ Sustainable Development Goals (UN SDGs) conveys the message that sustainability is not a "luxury" but an essential vehicle through which to pursue and guarantee everyone's rights, inclusion and future.Solving complex problems calls for collaboration among communities; aware of that, Italian universities set up the RUS - Italian University Network for Sustainable Development - which acts as a model to encourage collaboration between universities and cities, to spread social innovation at local level and to provide cultural awareness at national level. The University of Trieste (UniTs) joined the network in 2017. Consistent with its strategy for sustainability, UniTs has implemented measures on the macro-context (to raise public awareness and to support research) as well as on micro-context (to orientate behaviors and habits of its community). In particular, it focused on virtuous projects such as waste management, based both on the constant reduction of waste production and on the optimization of the use of materials and products (i.e. paper, glass, reagents), along with the setting of separate waste collection to reduce disposal and favor recycling. The paper will refer on the present framework and the implementation of new integrated projects, aimed at consolidating a community attentive and aware of sustainability

Prediction of Kick Count in Triathletes during Freestyle Swimming Session Using Inertial Sensor Technology

Monitoring sports training performances with automatic, low cost, low power, and ergonomic solutions is a topic of increasing importance in the research of the last years. A parameter of particular interest, which has not been extensively dealt with in a state-of-the-art way, is the count of kicks during swimming training sessions. Coaches and athletes set the training sessions to optimize the kick count and swim stroke rate to acquire velocity and acceleration during swimming. In regard to race distances, counting kicks can influence the athlete’s performance. However, it is difficult to record the kick count without facing some issues about subjective interpretation. In this paper, a new method for kick count is proposed, based on only one triaxial accelerometer worn on the athlete’s ankle. The algorithm was validated on data recorded during freestyle training sessions. An accuracy of 97.5% with a sensitivity of 99.3% was achieved. The proposed method shows good linearity and a slope of 1.01. These results overcome other state-of-the-art methods, proving that this method is a good candidate for a reliable, embedded kick count

Improvements of simplified hourly models for the energy assessment of buildings: The application of EN ISO 52016 in Italy

The Italian National Annex (NA) to EN ISO 52016-1 is aimed at improving the simplified hourly calculation method of the building thermal performance assessment by introducing different modelling options. The present work deepens the influence of the Italian NA improved method on the thermal energy needs for heating and cooling of a residential building-type in two different climate zones. In particular, the Italian NA improved options are applied one-at-the-time, and their effect is evaluated in comparison with the EN ISO 52016-1 standard method. The use of solar angle and time dependent correction factors for the total solar energy transmittance of glazing proved to be the most sensitive modelling option, among those analysed. Negligible variations were instead reported for the other tested options

Detection and Characterization of Porcine Sapelovirus in Italian Pig Farms

Porcine sapelovirus (PSV) belongs to the genus Sapelovirus of the family Picornaviridae. PSV infects pigs asymptomatically, but it can also cause severe neurologic, enteric, and respiratory symptoms or reproductive failure. Sapelovirus infections have been reported worldwide in pigs. The objective of this study was to investigate the presence and the prevalence of PSV in Italian swine farms in animals of different ages to clarify the occurrence of the infection and the genetic characteristics of circulating strains. In the present study, 92 pools of fecal samples, collected from pigs across three farms, were analyzed by Reverse Transcriptase-polymerase Chain Reaction-PCR (RT-PCR). Fecal pools from young growers (63/64) were found positive for Sapelovirus in all farms while detection in sows (4/28) was observed in only one farm. Phylogenetic analyses of the 19 partial capsid protein nucleotide sequences (VP1) (6\u20137 each farm) enable the classification of the virus sequences into three distinct clades and highlighted the high heterogeneity within one farm. The whole genome sequence obtained from one strain showed the highest correlation with the Italian strain detected in 2015. The study adds novel information about the circulation and heterogeneity of PSV strains in Italy and considering the movement of pigs across Europe would also be informative for other countries

Bridging thermodynamics and metrology in non-equilibrium Quantum Thermometry

Single-qubit thermometry presents the simplest tool to measure the temperature of thermal baths with reduced invasivity. At thermal equilibrium, the temperature uncertainty is linked to the heat capacity of the qubit, however the best precision is achieved outside equilibrium condition. Here, we discuss a way to generalize this relation in a non-equilibrium regime, taking into account purely quantum effects such as coherence. We support our findings with an experimental photonic simulation.Comment: 7 pages, 4 figure