A C18^{18}O study of the origin of the power-law nature in the IMF

We have performed C$^{18}$O ($J$=1--0) mapping observations of a $20'\times20'$ area of the OMC-1 region in the Orion A cloud. We identified 65 C$^{18}$O cores, which have mean radius, velocity width in FWHM, and LTE mass of 0.18$\pm$0.03 pc, 0.40$\pm$0.15 km s$^{-1}$, and 7.2$\pm$4.5 $M_\odot$, respectively. All the cores are most likely to be gravitationally bound by considering the uncertainty in the C$^{18}$O abundance. We derived a C$^{18}$O core mass function, which shows a power-law-like behavior above 5 $M_\odot$. The best-fit power-law index of $-2.3\pm0.3$ is consistent with those of the dense core mass functions and the stellar initial mass function (IMF) previously derived in the OMC-1 region. This agreement strongly suggests that the power-law form of the IMF has been already determined at the density of $\sim10^{3}$ cm$^{-3}$, traced by the C$^{18}$O ($J$=1--0) line.Comment: 17 pages, 4 figure

Similarity between the C18O (J=1-0) core mass function and the IMF in the S 140 region

We present the results of C18O(J=1-0) mapping observations of a 20'x18' area in the Lynds 1204 molecular cloud associated with the Sharpless 2-140 (S140) H II region. The C18O cube (alpha-delta-vLSR) data shows that there are three clumps with sizes of \sim 1 pc in the region. Two of them have peculiar red shifted velocity components at their edges, which can be interpreted as the results of the interaction between the cloud and the Cepheus Bubble. From the C18O cube data, the clumpfind identified 123 C18O cores, which have mean radius, velocity width in FWHM, and LTE mass of 0.36\pm0.07 pc, 0.37\pm0.09 km s-1, and 41\pm29 Msun, respectively. All the cores in S140 are most likely to be gravitationally bound by considering the uncertainty in the C18O abundance. We derived a C18O core mass function (CMF), which shows a power-law-like behavior above a turnover at 30 Msun. The best-fit power-law index of -2.1\pm0.2 is quite consistent with those of the IMF and the C18O CMF in the OMC-1 region by Ikeda & Kitamura (2009). Kramer et al. (1998) estimated the power-law index of -1.65 in S140 from the C18O(J=2-1) data, which is inconsistent with this study. However, the C18O(J=2-1) data are spatially limited to the central part of the cloud and are likely to be biased toward high-mass cores, leading to the flatter CMF. Consequently, this study and our previous one strongly support that the power-law form of the IMF has been already determined at the density of \sim 10^{3-4} cm^{-3}, traced by the C18O(J=1-0) line.Comment: 36 pages, 9 figures, accepted for the Astrophysical Journa

A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833

In Corynebacterium glutamicum, the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) has long been the only known glucose uptake system, but we recently found suppressor mutants emerging from a PTS-negative strain of C. glutamicum ATCC 31833 on glucose agar plates, and identified two alternative potential glucose uptake systems, the myo-inositol transporters encoded by iolT1 and iolT2. The expression of either gene renders the PTS-negative strain WT Delta ptsH capable of growing on glucose. In the present study, we found a suppressor strain that still grew on glucose even after the iolT1 and iolT2 genes were both disrupted under the PTS-negative background. Whole-genome sequencing of the suppressor strain SPH1 identified a G-to-T exchange at 134 bp upstream of the bglF gene encoding an EII component of the beta-glucoside-PTS, which is found in limited wild-type strains of C. glutamicum. Introduction of the mutation into strain WT Delta ptsH allowed the PTS-negative strain to grow on glucose. Reverse transcription-quantitative PCR analysis revealed that the mutation upregulates the bglF gene by approximately 11-fold. Overexpression of bglF under the gapA promoter in strain WT Delta ptsH rendered the strain capable of growing on glucose, and deletion of bglF in strain SPH1 abolished the growth again, proving that bglF is responsible for glucose uptake in the suppressor strain. Simultaneous disruption of three glucokinase genes, glk (Cgl2185, NCgl2105), ppgK (Cgl1910, NCgl1835), and Cgl2647 (NCgl2558), in strain SPH1 resulted in no growth on glucose. Plasmid-mediated expression of any of the three genes in the triple-knockout mutant restored the growth on glucose. These results indicate that C. glutamicum ATCC 31833 has an additional non-PTS glucose uptake route consisting of the bglF-specified EII permease and native glucokinases.ArticleAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 99(6):2741-2750 (2015)journal articl

Influence of brain-derived neurotrophic factor on pathfinding of dentate granule cell axons, the hippocampal mossy fibers

Mossy fibers, the dentate granule cell axons, are generated throughout an animal's lifetime. Mossy fiber paths and synapses are primarily restricted to the stratum lucidum within the CA3 region. Brain-derived neurotrophic factor (BDNF), a neurotrophin family protein that activates Trk neurotrophin receptors, is highly expressed in the stratum lucidum in an activity-dependent manner. The addition of a Trk neurotrophin receptor inhibitor, K252a, to cultured hippocampal slices induced aberrant extension of mossy fibers into ectopic regions. BDNF overexpression in granule cells ameliorated the mossy fiber pathway abnormalities caused by a submaximal dose of K252a. A similar rescue was observed when BDNF was expressed in CA3 pyramidal cells, most notably in mossy fibers distal to the expression site. These findings are the first to clarify the role of BDNF in mossy fiber pathfinding, not as an attractant cue but as a regulator, possibly acting in a paracrine manner. This effect of BDNF may be as a signal for new fibers to fasciculate and extend further to form synapses with neurons that are far from active BDNF-expressing synapses. This mechanism would ensure the emergence of new independent dentate gyrus-CA3 circuits by the axons of new-born granule cells

Thin film write head field analysis using a benchmark problem

A benchmark problem has been proposed by the Storage Research Consortium (SRC) in Japan, for evaluating the applicability of computer codes to 3-D nonlinear eddy current analysis of thin film magnetic recording write head. Various codes using the finite element method are compared in terms of the write head field and the computational efficiency. The difficulty in 3-D mesh generation of thin film head is also discussed. The write head fields calculated by various codes using different meshes show fairly good agreement. The calculated write head fields are verified by measurement using a stroboscopic electron beam tomography. It is found that the calculation time strongly depends on unknown variables </p

Overexpression of Nuclear Receptor 5A1 Induces and Maintains an Intermediate State of Conversion between Primed and Naive Pluripotency

Naive and primed human pluripotent stem cells (hPSCs) have provided useful insights into the regulation of pluripotency. However, the molecular mechanisms regulating naive conversion remain elusive. Here, we report intermediate naive conversion induced by overexpressing nuclear receptor 5A1 (NR5A1) in hPSCs. The cells displayed some naive features, such as clonogenicity, glycogen synthase kinase 3β, and mitogen-activated protein kinase (MAPK) independence, expression of naive-associated genes, and two activated X chromosomes, but lacked others, such as KLF17 expression, transforming growth factor β independence, and imprinted gene demethylation. Notably, NR5A1 negated MAPK activation by fibroblast growth factor 2, leading to cell-autonomous self-renewal independent of MAPK inhibition. These phenotypes may be associated with naive conversion, and were regulated by a DPPA2/4-dependent pathway that activates the selective expression of naive-associated genes. This study increases our understanding of the mechanisms regulating the conversion from primed to naive pluripotency

Physical Properties of Dense Cores in the Rho Ophiuchi Main Cloud and A Significant Role of External Pressures in Clustered Star Formation

Using the archive data of the H13CO+ (J=1-0) line emission taken with the Nobeyama 45 m radio telescope with a spatial resolution of about 0.01pc, we have identified 68 dense cores in the central dense region of the rho Ophiuchi main cloud. The H13CO+ data also indicates that the fractional abundance of H13CO+ relative to H2 is roughly inversely proportional to the square root of the H2 column density with a mean of 1.72 x 10^{-11}. The mean radius, FWHM line width, and LTE mass of the identified cores are estimated to be 0.045 +- 0.011 pc, 0.49 +- 0.14 km/s, and 3.4 +- 3.6 Msolar, respectively. The majority of the identified cores have subsonic internal motions. The virial ratio, the ratio of the virial mass to the LTE mass, tends to decrease with increasing the LTE mass and about 60 percent of the cores have virial ratios smaller than 2, indicating that these cores are not transient structures but self-gravitating. The detailed virial analysis suggests that the surface pressure often dominates over the self-gravity and thus plays a crucial role in regulating core formation and evolution. By comparing the rho Oph cores with those in the Orion A molecular cloud observed with the same telescope, we found that the statistical properties of the core physical quantities are similar between the two clouds if the effect of the different spatial resolutions is corrected. The line widths of the rho Oph cores appear to be nearly independent of the core radii over the range of 0.01 - 0.1 pc and deviate upwards from the Heyer & Brunt relation. This may be evidence that turbulent motions are driven by protostellar outflows in the cluster environment.Comment: 45 pages, 14 figures, accepted or publication in ApJ, mpeg movies of figure 3 are available from http://quasar1.ed.niigata-u.ac.jp/~fnakamur/papers/oph1