Production of Periwinkle Shell Reinforced Grindstone

The study involves the use of periwinkle shell particles as reinforcement in the production of grindstone to meliorate its physical and mechanical properties. The assessment of the properties of the periwinkle shell reinforced concrete grindstone showed that reinforcement yielded several promising results. The compressive strength was higher for samples with lower water/cement ratio (7%) than those with higher water/cement ratio (10%). The introduction of concrete that contains periwinkle shells as fine aggregates resulted in the loss of smooth like appearance and reduction in weight. The loss of its smooth like nature introduces the rough nature which improved its functionality. The reinforced sample had the lowest hardness test and in hardness test the lower the number the higher the hardness. There was an increase in the density after absorption test was carried out on specimens. There was also reduction in production cost with periwinkle shells partial replacement of cement and gravel as fine aggregate. The composite was adequate for grindstone production since its sample has a compressive of 18.15Mpa and thus satisfied MOR requirements of 11.5 MPa for general purpose boards

Effect of Fermentation on the Anti-Nutritional Factors and

The effect of fermentation on the anti-nutritional factors and mineral composition of melon seed varieties for Ogiri production was studied. Melon seed varieties such as Citrullus vulgaris, Citrulluslanatus, Colocynthiscitrullus, Cucurbita pepo, Cucurmeropisedulis were respectively sorted, washed, boiled wrapped seed were then boiled again for 2 hours, drained, cooled and allowed to ferment naturally for 86 hours (primary fermentation). The primary fermented sees were then pounded and wrapped in little portions with “ofoala” leaf (Icacinatrichantha olive) and kept in wire mesh near a heat source for another 144 hours (secondary fermentation). Samples were drawn from the raw, boiled and fermented melon seed varieties for the quantitative analysis of mineral content and anti-nutritional prepared with the raw and primary fermented samples. Raw seed of Citrulluslanatus had the highest mineral analysis showed a decline in the boiled samples and secondary fermented sample, compared with the raw and mineral composition ranging from potassium, magnesium, cacium, iron and zinc of 1.21, 1.06, 0.89, 0.45 and 0.41mg/100g respectively followed by raw Citrullus Vulgaris with potassium, magenesium, calcium, iron and zinc of 1.18, 1.02, 0.55, 0.44 and 0.38 mg/100g respectively and 1.11, 0.94, 0.81, 0.38 and 0.31 mg/100gof potassium, magenesium, calcium, iron and zinc respectively in the primary fermented product. Statistical analysis of anti-nutrients revealed a significant reduction (p<0.05) in all the processed melon. There was a significant difference in all the processed melon with lowest anti-nutrient content ranging from alkaloid, saponin, HCN, phytate, tannin and flavonoid (0.00, 0.00, 0.00, 0.00, 0.03and 0.09 respectively) and Colocynthiscitrullus had the highest anti-nutrient content in the secondary fermentation. Keywords:Fermentation, anti-nutritional factors, Ogiri, mineral content,melon seed varieties

Assessment of the Quality of Borehole Water Sample in Federal Housing Estate and Sites and Services Areas of Owerri, Imo State, Nigeria

Assessment of the quality of borehole water samples from Federal Housing Estate and Sites and Services areas of Owerri, Imo State, Nigeria was conducted to determine the suitability of these borehole water samples. Six samples of borehole water gotten from six different families living in these areas were analyzed for microbial, chemical and physicochemical parametersusing standard analytical method of National Agency for Food and Drug Administration and Control (NAFDAC). The result of microbial analysis revealed that all the water samples (samples D, E, F, G, H, and M) had total coliform count of 64.0cfu/100ml, 5.0cfu/100ml, 41.0cfu/100ml, 16.0cfu/100ml, 124.0cfu/100ml and 0.0cfu/100ml respectively. This showed that sample D, F, G, and H exceeded the standard of 10 coliform counts/100ml. The entire samples resulted at 0 counts for Escherichia coli. Samples D, F, G and M tested negative for pseudomonas test, whereas samples E and H did not. The chemical analysis showed that all the samples did not meet up with the recommended standard of pH (6.5-8.5).However, there was significant difference (p<0.5) between samples D and E. samples F and J were significantly the same, also as samples G and M. Sample M was the least significant while sample D was the most significant at pH 4.6 and 6.4 respectively. The temperatures were all significantly the same and did not exceed standard limit of 370C. The total dissolved solid also did not exceed the limit of 500ppm and the conductivity limit was not exceeded. All the samples did not exceed limits for zinc, copper, lead, magnesium, cadmium and iron which are 3mg/l, 1mg/l, 0.01mg/l, 0.02mg/l, 0.03mg/l and 0.3mg/l  respectively except for calcium, where samples D, E, G and H were beyond standard of 0.4mg/l. All the samples were significantly different for each parameter except for lead of which the entire sample were all the same. The depth of the borehole in relation to the distance of septic tank, distance of neighbor’s septic tank and distance of refuse dump as obtained from these families revealed that sample M had the highest distance and was the least contaminated, whereas sample H had the closest distance and the greatest microbial contamination. The depth of Sample G borehole was according to the regulatory standard of 150 ft., and from the analysis the entire parameters were within standard except for pH. Keywords: Water, Standards, Microbial analysis, Total Dissolved Solid, Metals, Septic tan

TRIP-1 via AKT modulation drives lung fibroblast/myofibroblast trans-differentiation

Abstract Background Myofibroblasts are the critical effector cells in the pathogenesis of pulmonary fibrosis which carries a high degree of morbidity and mortality. We have previously identified Type II TGFβ receptor interacting protein 1 (TRIP-1), through proteomic analysis, as a key regulator of collagen contraction in primary human lung fibroblasts—a functional characteristic of myofibroblasts, and the last, but critical step in the process of fibrosis. However, whether or not TRIP-1 modulates fibroblast trans-differentiation to myofibroblasts is not known. Methods TRIP-1 expression was altered in primary human lung fibroblasts by siRNA and plasmid transfection. Transfected fibroblasts were then analyzed for myofibroblast features and function such as α-SMA expression, collagen contraction ability, and resistance to apoptosis. Results The down-regulation of TRIP-1 expression in primary human lung fibroblasts induces α-SMA expression and enhances resistance to apoptosis and collagen contraction ability. In contrast, TRIP-1 over-expression inhibits α-SMA expression. Remarkably, the effects of the loss of TRIP-1 are not abrogated by blockage of TGFβ ligand activation of the Smad3 pathway or by Smad3 knockdown. Rather, a TRIP-1 mediated enhancement of AKT phosphorylation is the implicated pathway. In TRIP-1 knockdown fibroblasts, AKT inhibition prevents α-SMA induction, and transfection with a constitutively active AKT construct drives collagen contraction and decreases apoptosis. Conclusions TRIP-1 regulates fibroblast acquisition of phenotype and function associated with myofibroblasts. The importance of this finding is it suggests TRIP-1 expression could be a potential target in therapeutic strategy aimed against pathological fibrosis.Peer Reviewe

Pancreatic beta cell protection/regeneration with phytotherapy

Although currently available drugs are useful in controlling early onset complications of diabetes, serious late onset complications appear in a large number of patients. Considering the physiopathology of diabetes, preventing beta cell degeneration and stimulating the endogenous regeneration of islets will be essential approaches for the treatment of insulin-dependent diabetes mellitus. The current review focused on phytochemicals, the antidiabetic effect of which has been proved by pancreatic beta cell protection/regeneration. Among the hundreds of plants that have been investigated for diabetes, a small fraction has shown the regenerative property and was described in this paper. Processes of pancreatic beta cell degeneration and regeneration were described. Also, the proposed mechanisms for the protective/regenerative effects of such phytochemicals and their potential side effects were discussed

Identification of Antifungal Compounds Active against Candida albicans Using an Improved High-Throughput Caenorhabditis elegans Assay

Candida albicans, the most common human pathogenic fungus, can establish a persistent lethal infection in the intestine of the microscopic nematode Caenorhabditis elegans. The C. elegans–C. albicans infection model was previously adapted to screen for antifungal compounds. Modifications to this screen have been made to facilitate a high-throughput assay including co-inoculation of nematodes with C. albicans and instrumentation allowing precise dispensing of worms into assay wells, eliminating two labor-intensive steps. This high-throughput method was utilized to screen a library of 3,228 compounds represented by 1,948 bioactive compounds and 1,280 small molecules derived via diversity-oriented synthesis. Nineteen compounds were identified that conferred an increase in C. elegans survival, including most known antifungal compounds within the chemical library. In addition to seven clinically used antifungal compounds, twelve compounds were identified which are not primarily used as antifungal agents, including three immunosuppressive drugs. This assay also allowed the assessment of the relative minimal inhibitory concentration, the effective concentration in vivo, and the toxicity of the compound in a single assay

Twelve-month observational study of children with cancer in 41 countries during the COVID-19 pandemic

Introduction Childhood cancer is a leading cause of death. It is unclear whether the COVID-19 pandemic has impacted childhood cancer mortality. In this study, we aimed to establish all-cause mortality rates for childhood cancers during the COVID-19 pandemic and determine the factors associated with mortality. Methods Prospective cohort study in 109 institutions in 41 countries. Inclusion criteria: children <18 years who were newly diagnosed with or undergoing active treatment for acute lymphoblastic leukaemia, non-Hodgkin's lymphoma, Hodgkin lymphoma, retinoblastoma, Wilms tumour, glioma, osteosarcoma, Ewing sarcoma, rhabdomyosarcoma, medulloblastoma and neuroblastoma. Of 2327 cases, 2118 patients were included in the study. The primary outcome measure was all-cause mortality at 30 days, 90 days and 12 months. Results All-cause mortality was 3.4% (n=71/2084) at 30-day follow-up, 5.7% (n=113/1969) at 90-day follow-up and 13.0% (n=206/1581) at 12-month follow-up. The median time from diagnosis to multidisciplinary team (MDT) plan was longest in low-income countries (7 days, IQR 3-11). Multivariable analysis revealed several factors associated with 12-month mortality, including low-income (OR 6.99 (95% CI 2.49 to 19.68); p<0.001), lower middle income (OR 3.32 (95% CI 1.96 to 5.61); p<0.001) and upper middle income (OR 3.49 (95% CI 2.02 to 6.03); p<0.001) country status and chemotherapy (OR 0.55 (95% CI 0.36 to 0.86); p=0.008) and immunotherapy (OR 0.27 (95% CI 0.08 to 0.91); p=0.035) within 30 days from MDT plan. Multivariable analysis revealed laboratory-confirmed SARS-CoV-2 infection (OR 5.33 (95% CI 1.19 to 23.84); p=0.029) was associated with 30-day mortality. Conclusions Children with cancer are more likely to die within 30 days if infected with SARS-CoV-2. However, timely treatment reduced odds of death. This report provides crucial information to balance the benefits of providing anticancer therapy against the risks of SARS-CoV-2 infection in children with cancer

Oral rehydration therapies in Senegal, Mali, and Sierra Leone: a spatial analysis of changes over time and implications for policy.

BACKGROUND: Oral rehydration solution (ORS) is a simple intervention that can prevent childhood deaths from severe diarrhea and dehydration. In a previous study, we mapped the use of ORS treatment subnationally and found that ORS coverage increased over time, while the use of home-made alternatives or recommended home fluids (RHF) decreased, in many countries. These patterns were particularly striking within Senegal, Mali, and Sierra Leone. It was unclear, however, whether ORS replaced RHF in these locations or if children were left untreated, and if these patterns were associated with health policy changes. METHODS: We used a Bayesian geostatistical model and data from household surveys to map the percentage of children with diarrhea that received (1) any ORS, (2) only RHF, or (3) no oral rehydration treatment between 2000 and 2018. This approach allowed examination of whether RHF was replaced with ORS before and after interventions, policies, and external events that may have impacted healthcare access. RESULTS: We found that RHF was replaced with ORS in most Sierra Leone districts, except those most impacted by the Ebola outbreak. In addition, RHF was replaced in northern but not in southern Mali, and RHF was not replaced anywhere in Senegal. In Senegal, there was no statistical evidence that a national policy promoting ORS use was associated with increases in coverage. In Sierra Leone, ORS coverage increased following a national policy change that abolished health costs for children. CONCLUSIONS: Children in parts of Mali and Senegal have been left behind during ORS scale-up. Improved messaging on effective diarrhea treatment and/or increased ORS access such as through reducing treatment costs may be needed to prevent child deaths in these areas

Global respiratory syncytial virus–related infant community deaths

BACKGROUND : Respiratory syncytial virus (RSV) is a leading cause of pediatric death, with >99% of mortality occurring in low and lower middle-income countries. At least half of RSV-related deaths are estimated to occur in the community, but clinical characteristics of this group of children remain poorly characterized. METHODS : The RSV Global Online Mortality Database (RSV GOLD), a global registry of under-5 children who have died with RSV-related illness, describes clinical characteristics of children dying of RSV through global data sharing. RSV GOLD acts as a collaborative platform for global deaths, including community mortality studies described in this supplement. We aimed to compare the age distribution of infant deaths <6 months occurring in the community with in-hospital. RESULTS : We studied 829 RSV-related deaths <1 year of age from 38 developing countries, including 166 community deaths from 12 countries. There were 629 deaths that occurred <6 months, of which 156 (25%) occurred in the community. Among infants who died before 6 months of age, median age at death in the community (1.5 months; IQR: 0.8−3.3) was lower than in-hospital (2.4 months; IQR: 1.5−4.0; P < .0001). The proportion of neonatal deaths was higher in the community (29%, 46/156) than in-hospital (12%, 57/473, P < 0.0001). CONCLUSIONS : We observed that children in the community die at a younger age. We expect that maternal vaccination or immunoprophylaxis against RSV will have a larger impact on RSV-related mortality in the community than in-hospital. This case series of RSV-related community deaths, made possible through global data sharing, allowed us to assess the potential impact of future RSV vaccines.The Bill & Melinda Gates Foundation.am2023Medical Virolog

Global Respiratory Syncytial Virus-Related Infant Community Deaths.

BACKGROUND: Respiratory syncytial virus (RSV) is a leading cause of pediatric death, with >99% of mortality occurring in low- and lower middle-income countries. At least half of RSV-related deaths are estimated to occur in the community, but clinical characteristics of this group of children remain poorly characterized. METHODS: The RSV Global Online Mortality Database (RSV GOLD), a global registry of under-5 children who have died with RSV-related illness, describes clinical characteristics of children dying of RSV through global data sharing. RSV GOLD acts as a collaborative platform for global deaths, including community mortality studies described in this supplement. We aimed to compare the age distribution of infant deaths <6 months occurring in the community with in-hospital. RESULTS: We studied 829 RSV-related deaths <1 year of age from 38 developing countries, including 166 community deaths from 12 countries. There were 629 deaths that occurred <6 months, of which 156 (25%) occurred in the community. Among infants who died before 6 months of age, median age at death in the community (1.5 months; IQR: 0.8-3.3) was lower than in-hospital (2.4 months; IQR: 1.5-4.0; P < .0001). The proportion of neonatal deaths was higher in the community (29%, 46/156) than in-hospital (12%, 57/473, P < 0.0001). CONCLUSIONS: We observed that children in the community die at a younger age. We expect that maternal vaccination or immunoprophylaxis against RSV will have a larger impact on RSV-related mortality in the community than in-hospital. This case series of RSV-related community deaths, made possible through global data sharing, allowed us to assess the potential impact of future RSV vaccines