Endophytic Fungi as Alternative and Reliable Sources for Potent Anticancer Agents

In comparison with other natural sources like plants, highly diverse microorganisms are narrowly explored, especially with respect to their limitless potentials as repositories of exceptionally bioactive natural products. Of these organisms, fungi inhabiting tissues of plant in a noninvasive relationship (endophytic fungi) have proven undeniably useful and unmatchable as sources of potent bioactive molecules against several diseases such as cancer and related ailments. In general terms, endophytic fungi are highly prevalent organisms found in the tissue (intracellular or intercellular) of plants and at least for reasonable portion of their lives. It has been proven that virtually every plant, irrespective of habitat and climate, plays host to wide varieties of endophytes. Endophytic fungi produce metabolites produced by different biosynthetic pathways to that of the host plant, and this robustness equips them to synthesize unlimited structural entities and scaffolds of diverse classes. Interestingly too, the cohabitation/culture of these fungi with certain bacteria offers even stronger hopes for anticancer drug discovery. The endless need for potent drugs has necessitated the search of bioactive molecules from several sources, and endophytic fungi appear to be a recipe. This chapter is an attempt to present the current trend of research with these very promising organisms

Gender and Resource Productivity in Rice Production n Ebonyi State, Nigeria

Abstract: The inadequacy of information on resource productivity and their differentials by gender in rice production has prevented rice farmers from knowing the resources they are yet to realize their full potentials. This study analyzed resource productivity by gender in rice production in Ebonyi State of Nigeria. Multi-stage random sampling techniques were used to select a sample of 130 rice farmers comprised of 65 males and 65 females from the 13 Local government areas of Ebonyi State. Data were collected with structured and validated questionnaire and analyzed using descriptive statistics and productivity model. Results showed that marginal productivity of labour, capital and land for male farmers were higher than those of female farmers, while the marginal productivity of fertilizer and seed for female farmers were higher than those of male farmers. Rice farmers should be encouraged to use more of the resource imputs since a unit change in their use increases rice output

Effects of Household Waste Generation, Disposal and Management on Farmers’ Health in Owerri Metropolis of IMO State, Nigeria

The study investigated the effects of household waste generation, disposal and management on farmers' health in Owerri metropolis. It specifically ascertained the socio-economic characteristics of the farmers, identified the types and sources of waste in the study area, examined the waste disposal and management methods in the study area examined the effects of inappropriate waste disposal and ascertained the appropriate waste disposal methods used in the study area. A multi stage sampling technique was used to select one hundred and eight farmers from the three Local Government Area in Owerri metropolis. Data were collected using a validated questionnaire and were analyzed using descriptive statistics tool such as mean, frequency, percentage, and mean scores. Results show that waste is majorly generated from markets and residential homes. Waste disposal methods were mainly burning, landfills and open dumping. Its effects includes destroys the beauty of the environment, blocks gutters and drainage system, pollutes the environment among others. Subsequently, appropriate waste disposal methods in the study area includes burning of waste (29.17%), placing of bins at appropriate places (16.07%). The study recommends amongst others that waste management environmental agency should make waste dumps or receptacles accessible to residents, public campaigns should be embarked upon to educate the citizens on ills of dirty environment

A Comparison of Shiga-Toxin 2 Bacteriophage from Classical Enterohemorrhagic Escherichia coli Serotypes and the German E. coli O104:H4 Outbreak Strain

Escherichia coli O104:H4 was associated with a severe foodborne disease outbreak originating in Germany in May 2011. More than 4000 illnesses and 50 deaths were reported. The outbreak strain was a typical enteroaggregative E. coli (EAEC) that acquired an antibiotic resistance plasmid and a Shiga-toxin 2 (Stx2)-encoding bacteriophage. Based on whole-genome phylogenies, the O104:H4 strain was most closely related to other EAEC strains; however, Stx2-bacteriophage are mobile, and do not necessarily share an evolutionary history with their bacterial host. In this study, we analyzed Stx2-bacteriophage from the E. coli O104:H4 outbreak isolates and compared them to all available Stx2-bacteriophage sequences. We also compared Stx2 production by an E. coli O104:H4 outbreak-associated isolate (ON-2011) to that of E. coli O157:H7 strains EDL933 and Sakai. Among the E. coli Stx2-phage sequences studied, that from O111:H- strain JB1-95 was most closely related phylogenetically to the Stx2-phage from the O104:H4 outbreak isolates. The phylogeny of most other Stx2-phage was largely concordant with their bacterial host genomes. Finally, O104:H4 strain ON-2011 produced less Stx2 than E. coli O157:H7 strains EDL933 and Sakai in culture; however, when mitomycin C was added, ON-2011 produced significantly more toxin than the E. coli O157:H7 strains. The Stx2-phage from the E. coli O104:H4 outbreak strain and the Stx2-phage from O111:H- strain JB1-95 likely share a common ancestor. Incongruence between the phylogenies of the Stx2-phage and their host genomes suggest the recent Stx2-phage acquisition by E. coli O104:H4. The increase in Stx2-production by ON-2011 following mitomycin C treatment may or may not be related to the high rates of hemolytic uremic syndrome associated with the German outbreak strain. Further studies are required to determine whether the elevated Stx2-production levels are due to bacteriophage or E. coli O104:H4 host related factors

The Global Hidradenitis Suppurativa Atlas (GHiSA) methodology: Combining global proportions in a pooled analysis

Introduction: Data concerning the global burden of Hidradenitis Suppurativa (HS) are limited. Reported prevalence estimates vary between 0.0003% and 4.1%, and data from various geographical regions are still to be collected. Previously reported prevalence rates have been limited by the methodological approach and source of data. This has resulted in great heterogeneity as prevalence data from physician-diagnosed cases poorly match those of self-reported apparent HS disease. Methods: The Global Hidradenitis Suppurativa Atlas (GHiSA) introduces an innovative approach to determine the global prevalence of HS. This approach involves using a previously validated questionnaire to screen apparently healthy adults accompanying a patient to a non-dermatological outpatient clinic visit in a hospital. The screening questionnaire (i.e., the index test) is combined with a subsequent physician-based in-person validation (i.e., the reference standard) of the participants who screen positive. Ten percent of the screen-negative participants are also clinically assessed to verify the diagnostic precision of the test. The local prevalence (pi) will be estimated from each country that submits the number of patients who are HS positive according to the index test and clinical examination (n), and the corresponding total number of observations (N). Conclusion: The GHiSA Global Prevalence studies are currently running simultaneously in 58 countries across six continents (Africa, Europe, Australia, North America, South America, and Asia). The goal of the combined global proportion is the generation of a single summary (i.e., proportional meta-analysis), which will be done after a logit transformation, and synthesized using a random-effects model. The novel standardization of the Global Prevalence studies conducted through GHiSA enables direct international comparisons, which were previously not possible due to substantial heterogeneity in past HS prevalence studies

Psychosocial and cultural aspects of pseudocyesis

Though considered rare in the developed countries, pseudocyesis is fairly common in gynecological practices in Africa. Using a case report and an overview of the literature, this paper posits that the elucidation of the psychosocial and cultural contexts within which a given patient lives can provide a basis for the empathic understanding of the reasons for the development of pseudocyesis. The case underscores the contributions of extreme poverty, relationship instability, and recurrent partner abuse in the enactment of pseudocyesis within a culture that treasures children for economic survival and generational continuity. The awareness of this cultural dimension is considered relevant to effective clinical care

Maternal HIV seropositivity and perinatal/neonatal outcome at Ebonyi State University Teaching Hospital, Abakaliki, Nigeria

Context: HIV seropositivity in pregnancy has been an issue of major concern worldwide. This is because of the risk of mother to child transmission which is associated with adverse perinatal/neonatal outcome. Objective: To determine the maternal HIV seropositivity and perinatal/neonatal outcome. Study Design, Setting and Subjects: A retrospective audit of all cases of HIV seropositivity among the antenatal mothers seen at the Ebonyi State University Teaching Hospital, Abakaliki, over a three-year period (January 2001-December 2003) as well as the outcome of their babies was carried out. Results: Out of 9 865 women who registered for antenatal care during the study period, 1 704 (17.3%) were screened for HIV infection. Of these, 91 (5.3%) were confirmed positive. Twenty-four mothers (26.4%) absconded after diagnosis and 67 case files (73.6%) were available for analysis. The highest seropositive rate occurred in the age group 26-30 years constituting 41.8% (28/67) while primigravidae alone accounted for 28.4% (19/67). Most of the mothers were housewives (31.3%, 21/67) while their husbands were mainly farmers (37.4%, 25/67) and transporters (28.4%, 19/67). Fifteen mothers (22.4%) showed features suggestive of AIDS. Of the 66 babies, 49 (74.2%) were term, 17 (25.8%) were preterm while 56 (84.8%) were of low birth weight