Results of a major ambulatory oral surgery program using general inhalational anesthesia on disabled patients

Objectives: To assess the demographic characteristics and comorbidities of the group to be studied, as well as various quality indicators of a Major Ambulatory Surgery (MAS) program. Quantification of the surgical-anesthetic incidents. Study design: We aimed to perform a retrospective and descriptive analysis of disabled patients who had received oral ambulatory surgery under general anesthesia. Data obtained from the clinical history and telephone interview included the demographic characteristics, socioeconomic status, previous dental history, cause of the mental disability, degree of mental retardation, comorbidity measured according to the scale of the American Society of Anesthesiologists (ASA), anesthesia or preoperative surgical treatments, level of analgesia, length of stay, incidents in the Resuscitation Ward, the rate of substitution, suspensions, patients admitted, complications and the degree of patient satisfaction. Results: We included 112 oral surgery procedures performed on disabled patients who were treated under general inhalational anesthesia as part of MAS during the years 2006-2007. During this period, 577 restorations, 413 extractions, 179 sealants, 102 pulpectomies, 22 root canal treatments, 17 gingivectomies and 3 frenectomies were performed. A total of 75% (78 cases) of the patients had coexisting medical pathology. The average surgery time per patient was 72.69 ±29.78 minutes. The rate of replacement was 100%. The rate of suspension was 1.92%. The percentage of patients readmitted was 1.92%,due to significant bleeding in the mouth, which did not require treatment and the patients were discharged from hospital 24 hours after being admitted. The rate of patients who required re-hospitalization was 3.84%. Conclusions: The MAS performed in this group, despite being on patients with high comorbidity resulted in only a low number of medical incidents reporte

Aquaporins can be involved in the swelling caused by shiga toxin type 2 on hgec and hk-2 cells

Hemolytic uremic syndrome related to Shiga toxin?producing Escherichiacoli (STEC-HUS) is the principal etiology of acute kidney injuryin children in Argentina.Previously, we demonstrated that Shiga toxin type 2 (Stx2) damageshuman glomerular endothelial cells (HGEC) and HK-2 human proximaltubular epithelial cell line by inducing swelling and detachment.In this work, we analyzed cell volume changes of HGEC and HK-2exposed or not to Stx2 or a hypoosmotic (HYPO) medium, in thepresence or not of aquaporins (AQPs) inhibitors (mercuric chloride:HgCl2 and tetraethylammonium: TEA), or an inhibitor of Stx2 receptor(Gb3) synthesis, Eliglustat (EG). For controls, an isosmotic (ISO)medium was used.Cells were grown on 12 well plates and pretreated for 30 minuteswith HgCl2 (10 μM) or TEA (100 μM) or pretreated during 24 h withEG (10 μM). Then, HGEC and HK-2 were incubated with Stx2 (50μM) for an additional 40 minutes. Cell volume was analyzed by lightmicroscopy and measuring cell area by using Image J software.After Stx2 and HYPO medium treatments, a significant increase inthe cell volume of HGEC (Stx2: 42%; HYPO: 36%, n= 3, p<0.05) andHK-2 (Stx2: 70%; HYPO: 55%, n= 3, p<0.05) was detected respectto ISO medium. However, when HGEC and HK-2 were pretreatedwith HgCl2 or TEA a significant swelling prevention was obtained forHGEC (Stx2+HgCl2:100%; Stx2+TEA:86%; HYPO+HgCl2: 42.5%;HYPO+TEA: 83%, n=3, p<0.05) and HK-2 (Stx2+HgCl2: 90%; Stx-2+TEA: 85%; HYPO+HgCl2: 55%; HYPO+TEA: 75%, n=3, p<0.05).In addition, EG also was able to prevent HK-2 swelling in 87 % (n=1)with respect to Stx2 treatment.Results show that AQPs may be involved in the water movement insideHGEC and HK-2 induced by Stx2, since HgCl2 and TEA avoidedthis effect. Furthermore, binding of Stx2 to Gb3 could be the initialstep for the development of cellular mechanisms that possibly triggerthe entry of solutes into the cells and the consequent osmoticgradient responsible for the hypotonic effect.Fil: Gomez, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Repetti, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Romero, Romina. Hospital Nacional Profesor Alejandro Posadas.; ArgentinaFil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaLXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica: LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de NanomedicinasArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Nanomedicina

Adoption of improved varieties in adverse climatic conditions: Evidence from Nicaragua

This presentation summarizes the main results of a study made in Nicaragua to estimate the adoption of improved bean varieties (IVs) across different climatic environments, and the yield differences between adopters and non-adopters. We implemented a farmer-level survey representative of the most productive bean regions in the country. The results show that when comparing dry vs. non-dry regions ("corridors"), yields are 10.9% lower in the dry region; 29-31% of farmers have adopted IVs in non-dry and dry regions, respectively; and 28-29% of the bean area was planted to IVs in non-dry and dry regions, respectively. Although IV yields are statistically the same to non-IV yields (farmer-reported use of IVs), in dry regions, improved bean varieties yield 14.7% more than non-IVs, which is enough to feed 6-7 people for a year. This yield advantage (of IVs) dissapears under extreme dry conditions

La complejidad del gambito legal al gambito literario: ¿Quién debe ganar?

This article develops an approach to a social, literary and artistic phenomenon based on complexity and epistemology. The phenomenon refers to a standing lawsuit that has been filed to the producers of the Netflix series The Queen's Gambit. The producers are accused of a misrepresentation or “false” representation of the chess player Nona Gaprindashvili in the fictional universe of the series during the last episode. In this sense, the article examines the implications that this lawsuit, coming from outside the artistic work, bursts into the interior of the series and extends its effects to the multiple intertexts or universes of potential realities, to recriminate the misrepresentation of living subjects inside a fictional world. That is to say, the paper observes the multidimensional blend between reality and fiction, based on this phenomenon and how one affects the other, in addition to its scope.En este artículo se elabora una aproximación a un fenómeno social, literario y artístico a partir de la complejidad y la epistemología. El fenómeno consiste en una demanda legal vigente a principios de 2022 que ha sido impuesta a los productores de la serie The Queen’s Gambit de Netflix. A los productores se les imputa una “mala” o “falsa” representación de la ajedrecista Nona Gaprindashvili en el universo ficcional desplegado por la serie durante un instante en el último episodio. En este sentido, el artículo revisa las implicaciones que este señalamiento legal, proveniente del exterior de la obra, irrumpe en el interior de la serie y se extiende a los múltiples intertextos o universos de realidades potenciales, para recriminar representaciones “incorrectas” de sujetos vivos en un mundo ficcional. Es decir, se estudia la mezcla multidimensional entre la realidad y la ficción, a partir de este fenómeno y cómo repercute una en la otra, además de sus alcances

Amphibia, Anura, Centrolenidae Teratohyla midas (Lynch and Duellman, 1973) and Cochranella resplendens (Lynch and Duellman, 1973): First and second record respectively for Colombia

We present the first record of the glassfrog Teratohyla midas (Lynch and Duellman, 1973) for Colombia. This species, previously known in Ecuador, Peru and French Guiana, was collected at the municipalities of Belén de los Andaquíes and Florencia, Caquetá, Colombia. We also present the second record of Cochranella resplendens (Lynch and Duellman, 1973) for Colombia. The species was previously known from Santa Maria de Sucumbíos, department of Putumayo, Colombia

Metabolic syndrome improvement in depression six months after prescribing simple hygienic-dietary recommendations

BACKGROUND: Changes in diet and exercise have been separately demonstrated to improve Depression, although scientific evidence available is scarce. In a previously published controlled study, just recommending these and other lifestyle measures (sleep restriction and sunlight exposure) in combination once, patients experienced improvements in their depressive symptoms six months later. In this sample, one in three depressive patients had metabolic syndrome (MetS) at baseline. First line treatment of MetS condition is hygienic-dietetic, being Mediterranean diet and exercise especially important. Therefore we analyzed if lifestyle recommendations also improved their metabolic profile. FINDINGS: During the sixth month evaluation, a smaller number of patients from the group receiving hygienic-dietary recommendations met MetS criteria comparing with the control group. CONCLUSIONS: This study suggests that costless lifestyle recommendations, such as exercise and Mediterranean diet, have the capacity to promote both mental and physical health in a significant proportion of depressive patients. Further research is needed to confirm or discard these preliminary findings

Development and characterization of soluplus® nanomicelles associated to specific igg as an innovative strategy for the detection and neutralization of shiga toxin type 2

Shiga toxin type 2 (Stx2) is the main virulence factor of Shiga toxinproducing Escherichia coli and is responsible for triggering HemolyticUremic Syndrome (HUS). We aimed to develop and characterizepolymeric nanomicelles (PN) with the amphiphilic polymerSoluplus® coupled to anti-Stx2 IgG in order to introduce innovativeproposals for the detection of Stx2 and treatment of HUS. PN ofSoluplus® were formulated in PBS and coupled with IgG anti Stx2from hyperimmune (PN-IgG-Stx2) or control bovine colostrum (PNIgG-Ctrl). The hydrodynamic size of PN, PN-IgG-Stx2 and PN-IgGCtrlwas evaluated by Dynamic Light Scattering. Morphology of PNor PN-IgG-Stx2 was analyzed by Transmission Electron Microscopy(TEM). The PN toxicity was evaluated on both Vero and Human GlomerularEndothelial cells (HGEC) and cell viability was determinedby neutral red uptake. After coupling PN with IgG, Stx2 neutralizationcapacity of PN-IgG-Stx2 or PN-IgG-Ctrl was evaluated on Vero andHGEC cells and the percentage of cell viability was analyzed. Thehydrodynamic size of the PN of Soluplus® and IgG-Stx2 showed anaverage diameter of 70.2 ± 1.5 nm and 40.9 ± 2 nm, respectively.When both components were coupled, a single peak with a similarhydrodynamic size of the PN was observed (70.4 ± 0.2 nm). TEManalysis revealed circular particles with a diameter corresponding to100 nm either in PN and PN-IgG-Stx2 particles. PN-IgG-Stx2 wereable to neutralize Stx2 on Vero and HGEC cells in a dose dependentmanner. When comparing the neutralization capacity of Stx2 by IgGStx2vs PN-IgG-Stx2 a significant improvement in the cell viabilityof Vero and HGEC was observed with the PN-IgG-Stx2 (p<0.001).The association between anti-Stx2 IgG from bovine colostrum andSoluplus® PN was optimized and characterized. Encouraging resultsof antibody functionality coupled with PN were registered.These results may open the perspective of the design of new nanoplatformsfor neutralization and/or detection of Stx2.Fil: Girón Reyes, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Gomez, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Chiappetta, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Moretton, Marcela Analía. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaLXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de NanomedicinasArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Nanomedicina

The Mediterranean diet and micronutrient levels in depressive patients

Introduction: An inverse association between depression and some serum micronutrient levels (selenium, zinc, iron, magnesium, vitamin B and folic acid) has been reported. In addition, other studies reported that this micronutrient supplementation may improve depressed mood. The Mediterranean diet contains a sufficient amount of the micronutrients mentioned, although no study has reported an association between diet prescription and increased levels of them in depressive patients. Objective: To examine the impact of dietary patterns recommendations on micronutrient levels in depressive patients. Methods: 77 outpatients were randomly assigned either to the active (hygienic-dietary recommendations on diet, exercise, sleep, and sun exposure) or control group. Outcome measures were assessed before and after the six month intervention period. Results: Serum selenium and zinc levels were slightly low at basal point and serum selenium was inversely correlated with severity of depression (r=-0.233; p=0.041). A better outcome of depressive symptoms was found in the active group. Nevertheless, no significant differences in micronutrient levels were observed after the Mediterranean diet pattern prescription, probably due to an insufficient adherence. Conclusion: Selenium, zinc, iron, magnesium, vitamin B12 and folic acid serum levels didn`t increase in depressed patients after six months of the Mediterranean diet pattern prescription

Actualización de registros y distribución de Digitonthophagus gazella (Coleoptera: Scarabaeidae) en Argentina: Una potencial especie invasora

The distribution of Digitonthophagus gazella in Argentina is updated. A total of 246 individuals were captured between 2012 and 2022, through different techniques, including manual collection, light traps, and baited pitfall traps. The species is present in ten provinces, six of which are first records. In addition, D. gazella was recorded for the first time in five ecoregions, Atlantic Forest, the Espinal, Subtropical Grasslands, Northern Monte, and Parana Delta. The potential consequences of this introduced species on native dung beetles assemblages should be assessed in future studies.Se actualiza la distribución de Digitonthophagus gazella en Argentina. Se capturaron 246 individuos entre 2012 y 2022, por medio de diversas técnicas, colecta manual, trampas de luz y trampas de caída cebadas con diferentes tipos de excrementos. Esta especie fue recolectada en diez provincias, de las cuales seis de ellas constituyen primeros registros. Además, D. gazella se registra por primera vez en cinco ecorregiones, Bosque Atlántico, Espinal, Pastizal Subtropical, Monte Norte y Delta del Paraná. Las consecuencias potenciales de esta especie introducida sobre los ensambles de escarabajos coprófagos nativos, deben evaluarse en futuros estudios.Fil: Guerra Alonso, Celeste Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; ArgentinaFil: Ibarra Polesel, Mario Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura. Laboratorio de Biología de los Artrópodos; ArgentinaFil: Quiroga, Gabriel. Universidad Nacional de San Juan; ArgentinaFil: Bobadilla, Yanina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura. Laboratorio de Biología de los Artrópodos; ArgentinaFil: Gomez Cifuentes, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; ArgentinaFil: Giannoni, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Centro de Investigaciones de la Geosfera y Biosfera. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones de la Geosfera y Biosfera; ArgentinaFil: Zurita, Gustavo Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentin