Two-band superfluidity from the BCS to the BEC limit

We analyze the evolution of two-band superfluidity from the weak coupling Bardeen-Cooper-Schrieffer (BCS) to the strong coupling Bose-Einstein condensation (BEC) limit. When the interband interaction is tuned from negative to positive values, a quantum phase transition occurs from a 0-phase to a $\pi$-phase state, depending on the relative phase of two order parameters. Furthermore, population imbalances between the two bands can be created by tuning the intraband or interband interactions. We also find two undamped low energy collective excitations corresponding to in-phase and out-of-phase modes. Lastly, we derive the coupled Ginzburg-Landau equations, and show that they reduce to coupled Gross-Pitaevskii equations for two types of bosons in the BEC limit.Comment: 4 pages and 3 figure

BCS-BEC crossover of collective excitations in two-band superfluids

We use the functional integral approach to study low energy collective excitations in a continuum model of neutral two-band superfluids at T=0 for all couplings with a separable pairing interaction. In the long wavelength and low frequency limit, we recover Leggett's analytical results in weak coupling (BCS) for s-wave pairing, and further obtain analytical results in strong coupling (BEC) for both two and three dimensional systems. We also analyse numerically the behavior of the out-of-phase {\it exciton} (finite frequency) mode and the in-phase {\it phonon} (Goldstone) mode from weak to strong coupling limits, including the crossover region. In principle, the evolution of Goldstone and finite frequency modes from weak to strong coupling may be accessible experimentally in the superfluid phase of neutral Fermi atomic gases, and could serve as a test of the validity of the theoretical analysis and approximations proposed here.Comment: 14 pages, 9 figures. Submitted to PR

FUNCTIONAL CHARACTERIZATION OF THE ENDOCYTIC PROTEIN EPSIN3 IN BREAST CANCER

Endocytosis plays a critical role in the maintenance of cellular homeostasis. Due to its ability to resolve extracellular signals, the endocytic pathway controls many cellular processes, including transcription, proliferation, cell migration and cell fate determination. One relevant, unanswered, question regarding the role of endocytosis in the cell is whether subversion of the endocytic route is involved in the development of pathological conditions, such as cancer. This possibility is supported by studies showing altered expression of several endocytic proteins in human tumors. In a previous study in our lab, the endocytic protein Epsin 3 (Epn3) was found in a gene signature prognostic for metastatic breast cancer. Epn3 belongs to the Epsin family of endocytic proteins. Unlike the other Epsin members, Epn1 and Epn2, which are ubiquitously expressed, Epn3 is exclusively expressed in gastric cells and in wounded or pathological tissues exhibiting altered cell-extracellular matrix interactions. While Epn1 and Epn2 have been well characterized as endocytic adaptors, the exact function of Epn3 protein in endocytosis or signaling is largely unknown. In the present study, we show that Epn3 is overexpressed in approximately 30% of breast tumors and that upregulation of this protein correlates with clinico-pathological parameters of aggressive disease. We also show that EPN3 gene is amplified in human breast tumors and that this genetic alteration can occur independently from HER2 amplification in these tumors. Through functional studies using in vitro and in vivo breast cancer models, we demonstrate that breast tumor cells harboring Epn3 amplification/overexpression are dependent on Epn3 deregulation for the maintenance of their tumorigenic potential. In agreement with a possible oncogenic role, overexpression of Epn3 in a breast tumor cell line increases the tumorigenic potential in vivo. Of note, Epn3 overexpression is also able to induce, both in normal and tumor breast cells, transcriptional and morphological changes typical of an epithelial-to-mesenchymal transition (EMT), in a TGF\u3b2 12dependent manner; indeed, Epn3 overexpression induces upregulation of TGF\u3b2 ligands and receptors and inhibition of TGF\u3b2 signaling is able to revert Epn3-induced EMT. In addition, we show that Epn3 increases the capacity of normal mammary epithelial cells to form mammospheres in vitro, suggesting that Epn3 may contribute to the expansion of the breast cancer stem cell compartment. Finally, we demonstrate that Epn3 is involved in E-cadherin internalization, by inducing its downregulation from the cell surface upon TGF\u3b2-stimulation. In conclusion, our data suggest a novel oncogenic role for Epn3 in human breast cancer, through its action as an endocytic protein on E-cadherin trafficking. Overexpression of Epn3 might enhance E-cadherin internalization, and consequently induce EMT activating the expression of mesenchymal proteins that promote cell invasion and lead to tumor aggressiveness

Synthetic and Evolutionary Construction of a Chlorate-Reducing Shewanella oneidensis MR-1.

UnlabelledDespite evidence for the prevalence of horizontal gene transfer of respiratory genes, little is known about how pathways functionally integrate within new hosts. One example of a mobile respiratory metabolism is bacterial chlorate reduction, which is frequently encoded on composite transposons. This implies that the essential components of the metabolism are encoded on these mobile elements. To test this, we heterologously expressed genes for chlorate reduction from Shewanella algae ACDC in the non-chlorate-reducing Shewanella oneidensis MR-1. The construct that ultimately endowed robust growth on chlorate included cld, a cytochrome c gene, clrABDC, and two genes of unknown function. Although strain MR-1 was unable to grow on chlorate after initial insertion of these genes into the chromosome, 11 derived strains capable of chlorate respiration were obtained through adaptive evolution. Genome resequencing indicated that all of the evolved chlorate-reducing strains replicated a large genomic region containing chlorate reduction genes. Contraction in copy number and loss of the ability to reduce chlorate were also observed, indicating that this phenomenon was extremely dynamic. Although most strains contained more than six copies of the replicated region, a single strain with less duplication also grew rapidly. This strain contained three additional mutations that we hypothesized compensated for the low copy number. We remade the mutations combinatorially in the unevolved strain and determined that a single nucleotide polymorphism (SNP) upstream of cld enabled growth on chlorate and was epistatic to a second base pair change in the NarP binding sequence between narQP and nrfA that enhanced growth.ImportanceThe ability of chlorate reduction composite transposons to form functional metabolisms after transfer to a new host is an important part of their propagation. To study this phenomenon, we engineered Shewanella oneidensis MR-1 into a chlorate reducer. We defined a set of genes sufficient to endow growth on chlorate from a plasmid, but found that chromosomal insertion of these genes was nonfunctional. Evolution of this inoperative strain into a chlorate reducer showed that tandem duplication was a dominant mechanism of activation. While copy number changes are a relatively rapid way of increasing gene dosage, replicating almost 1 megabase of extra DNA is costly. Mutations that alleviate the need for high copy number are expected to arise and eventually predominate, and we identified a single nucleotide polymorphism (SNP) that relieved the copy number requirement. This study uses both rational and evolutionary approaches to gain insight into the evolution of a fascinating respiratory metabolism

Neuropsychology of posteromedial parietal cortex and conversion factors from Mild Cognitive Impairment to Alzheimer’s disease: systematic search and state-of-the-art review

In the present review, we discuss the rationale and the clinical implications of assessing visuospatial working memory (VSWM), awareness of memory deficits, and visuomotor control in patients with mild cognitive impairment (MCI). These three domains are related to neural activity in the posteromedial parietal cortex (PMC) whose hypoactivation seems to be a significant predictor of conversion from MCI to Alzheimer’s disease (AD) as indicated by recent neuroimaging evidence. A systematic literature search was performed up to May 2021. Forty-eight studies were included: 42 studies provided analytical cross-sectional data and 6 studies longitudinal data on conversion rates. Overall, these studies showed that patients with MCI performed worse than healthy controls in tasks assessing VSWM, awareness of memory deficits, and visuomotor control; in some cases, MCI patients’ performance was comparable to that of patients with overt dementia. Deficits in VSWM and metamemory appear to be significant predictors of conversion. No study explored the relationship between visuomotor control and conversion. Nevertheless, it has been speculated that the assessment of visuomotor abilities in subjects at high AD risk might be useful to discriminate patients who are likely to convert from those who are not. Being able to indirectly estimate PMC functioning through quick and easy neuropsychological tasks in outpatient settings may improve diagnostic and prognostic accuracy, and therefore, the quality of the MCI patient’s management

(Per)chlorate-reducing bacteria can utilize aerobic and anaerobic pathways of aromatic degradation with (per)chlorate as an electron acceptor.

UnlabelledThe pathways involved in aromatic compound oxidation under perchlorate and chlorate [collectively known as (per)chlorate]-reducing conditions are poorly understood. Previous studies suggest that these are oxygenase-dependent pathways involving O2 biogenically produced during (per)chlorate respiration. Recently, we described Sedimenticola selenatireducens CUZ and Dechloromarinus chlorophilus NSS, which oxidized phenylacetate and benzoate, two key intermediates in aromatic compound catabolism, coupled to the reduction of perchlorate or chlorate, respectively, and nitrate. While strain CUZ also oxidized benzoate and phenylacetate with oxygen as an electron acceptor, strain NSS oxidized only the latter, even at a very low oxygen concentration (1%, vol/vol). Strains CUZ and NSS contain similar genes for both the anaerobic and aerobic-hybrid pathways of benzoate and phenylacetate degradation; however, the key genes (paaABCD) encoding the epoxidase of the aerobic-hybrid phenylacetate pathway were not found in either genome. By using transcriptomics and proteomics, as well as by monitoring metabolic intermediates, we investigated the utilization of the anaerobic and aerobic-hybrid pathways on different electron acceptors. For strain CUZ, the results indicated utilization of the anaerobic pathways with perchlorate and nitrate as electron acceptors and of the aerobic-hybrid pathways in the presence of oxygen. In contrast, proteomic results suggest that strain NSS may use a combination of the anaerobic and aerobic-hybrid pathways when growing on phenylacetate with chlorate. Though microbial (per)chlorate reduction produces molecular oxygen through the dismutation of chlorite (ClO2(-)), this study demonstrates that anaerobic pathways for the degradation of aromatics can still be utilized by these novel organisms.ImportanceS. selenatireducens CUZ and D. chlorophilus NSS are (per)chlorate- and chlorate-reducing bacteria, respectively, whose genomes encode both anaerobic and aerobic-hybrid pathways for the degradation of phenylacetate and benzoate. Previous studies have shown that (per)chlorate-reducing bacteria and chlorate-reducing bacteria (CRB) can use aerobic pathways to oxidize aromatic compounds in otherwise anoxic environments by capturing the oxygen produced from chlorite dismutation. In contrast, we demonstrate that S. selenatireducens CUZ is the first perchlorate reducer known to utilize anaerobic aromatic degradation pathways with perchlorate as an electron acceptor and that it does so in preference over the aerobic-hybrid pathways, regardless of any oxygen produced from chlorite dismutation. D. chlorophilus NSS, on the other hand, may be carrying out anaerobic and aerobic-hybrid processes simultaneously. Concurrent use of anaerobic and aerobic pathways has not been previously reported for other CRB or any microorganisms that encode similar pathways of phenylacetate or benzoate degradation and may be advantageous in low-oxygen environments

Preserved Extra-Foveal Processing of Object Semantics in Alzheimer’s Disease

Alzheimer’s disease (AD) patients underperform on a range of tasks requiring semantic processing, but it is unclear whether this impairment is due to a generalised loss of semantic knowledge or to issues in accessing and selecting such information from memory. The objective of this eye-tracking visual search study was to determine whether semantic expectancy mechanisms known to support object recognition in healthy adults are preserved in AD patients. Furthermore, as AD patients are often reported to be impaired in accessing information in extra-foveal vision, we investigated whether that was also the case in our study. Twenty AD patients and 20 age-matched controls searched for a target object among an array of distractors presented extra-foveally. The distractors were either semantically related or unrelated to the target (e.g., a car in an array with other vehicles or kitchen items). Results showed that semantically related objects were detected with more difficulty than semantically unrelated objects by both groups, but more markedly by the AD group. Participants looked earlier and for longer at the critical objects when these were semantically unrelated to the distractors. Our findings show that AD patients can process the semantics of objects and access it in extra-foveal vision. This suggests that their impairments in semantic processing may reflect difficulties in accessing semantic information rather than a generalised loss of semantic memory

Vortex Imaging in the pi-Band of Magnesium Diboride

We report scanning tunneling spectroscopy imaging of the vortex lattice in single crystalline MgB2. By tunneling parallel to the c-axis, a single superconducting gap (Delta = 2.2 meV) associated with the pi-band is observed. The vortices in the pi-band have a large core size compared to estimates based on Hc2, and show an absence of localized states in the core. Furthermore, superconductivity between the vortices is rapidly suppressed by an applied field. These results suggest that superconductivity in the pi-band is, at least partially, induced by the intrinsically superconducting sigma-band.Comment: 4 pages, 3 figure

Significant modifications of the salivary proteome potentially associated with complications of Down syndrome revealed by top-down proteomics

People with Down syndrome, a frequent genetic disorder in humans, have increased risk of health problems associated with this condition. One clinical feature of Down syndrome is the increased prevalence and severity of periodontal disease in comparison with the general population. Because saliva plays an important role in maintaining oral health, in the present study the salivary proteome of Down syndrome subjects was investigated to explore modifications with respect to healthy subjects. Whole saliva of 36 Down syndrome subjects, divided in the age groups 10-17 yr and 18-50 yr, was analyzed by a top-down proteomic approach, based on the high performance liquid chromatography-electrospray ionization-MS analysis of the intact proteins and peptides, and the qualitative and quantitative profiles were compared with sex- and age-matched control groups. The results showed the following interesting features: 1) as opposed to controls, in Down syndrome subjects the concentration of the major salivary proteins of gland origin did not increase with age; as a consequence concentration of acidic proline rich proteins and S cystatins were found significantly reduced in older Down syndrome subjects with respect to matched controls; 2) levels of the antimicrobial α-defensins 1 and 2 and histatins 3 and 5 were significantly increased in whole saliva of older Down syndrome subjects with respect to controls; 3) S100A7, S100A8, and S100A12 levels were significantly increased in whole saliva of Down syndrome subjects in comparison with controls. The increased level of S100A7 and S100A12 may be of particular interest as a biomarker of early onset Alzheimer's disease, which is frequently associated with Down syndrome