A new case of centric fusion in cattle : Rob(21;23)

Centric fusions, also called Robertsonian translocations, are the most frequent chromosomal anomalies in cattle and they are responsible for economic losses due to fertility reduction in heterozygous carriers. Rob(1;29) is the most common, being found in more than 40 different breeds.1 To date, all bovine autosomes have been found to be involved in one or more centric fusions (Table S1). Here we show the involvement of BTA21 and BTA23 in a new centric fusion in the Maremmana breed by both conventional banding techniques and FISH analysis. This is the only centric fusion discovered in this Italian breed [except for rob (1;29)]

An improved characterization of horse ( Equus caballus

Abstract Peripheral blood lymphocytes were cultured and treated for early- and late-BrdU incorporation to perform replicating G- and R-banding patterns, respectively. Slides were treated for GBG-, RBA- and RBG-banding techniques. Improved banded karyotypes at early- (350 bands) and pro-metaphase (500 bands) stage were performed and GBG- and RBA-banded prometaphase karyotypes were presented for the first time on this species. All chromosomes, including the small acrocentrics, show clear and distinguishable G- and R-banding patterns. Chromosome identification followed the latest chromosome standard nomenclature (ISCNH 1997). This study is also our contribution to further standard karyotype attempts at the prometaphase stage