Structural features and immunological perception of the cell surface glycans of Lactobacillus plantarum: a novel rhamnose-rich polysaccharide and teichoic acids

Abstract The capsular material from Lactobacillus plantarum IMB19, an isolate from fermented vegetables, has been analyzed and our results demonstrate that most of the coat of this bacterium consists of glycerol- and ribitol-type teichoic acids, further decorated with other substituents (α-glucose and alanine), and of a capsular polysaccharide (CPS) with a linear nonasaccharide repeating unit, rich in rhamnose, interconnected to the next via a phosphodiester bridge. Stimulation of immune cells with the total capsular material resulted in the enhancement of immunostimulatory (IFNγ, TNF-α, IL-6 and IL-12) or immuno-regulatory (IL-10) cytokines in an in vitro splenocyte culture system. The capsular polysaccharide, and not the teichoic acids mixture, was responsible for the IFNγ production. Indeed, a significant increase of IFNγ along with other inflammatory cytokines, and a dose response in IFNγ expression with an EC50 of 3.16 μM was found for CPS, disclosing that this polysaccharide is a potent immunostimulatory molecule

The Host Galaxy and Optical Light Curve of the Gamma-Ray Burst GRB 980703

We present deep HST/STIS and ground-based photometry of the host galaxy of the gamma-ray burst GRB 980703 taken 17, 551, 710, and 716 days after the burst. We find that the host is a blue, slightly over-luminous galaxy with V_gal = 23.00 +/- 0.10, (V-R)_gal = 0.43 +/- 0.13, and a centre that is approximately 0.2 mag bluer than the outer regions of the galaxy. The galaxy has a star-formation rate of 8-13 M_sun/yr, assuming no extinction in the host. We find that the galaxy is best fit by a Sersic R^(1/n) profile with n ~= 1.0 and a half-light radius of 0.13 arcsec (= 0.72/h_100 proper kpc). This corresponds to an exponential disk with a scale radius of 0.22 arcsec (= 1.21/h_100 proper kpc). Subtracting a fit with elliptical isophotes leaves large residuals, which suggests that the host galaxy has a somewhat irregular morphology, but we are unable to connect the location of GRB 980703 with any special features in the host. The host galaxy appears to be a typical example of a compact star forming galaxy similar to those found in the Hubble Deep Field North. The R-band light curve of the optical afterglow associated with this gamma-ray burst is consistent with a single power-law decay having a slope of alpha = -1.37 +/- 0.14. Due to the bright underlying host galaxy the late time properties of the light-curve are very poorly constrained. The decay of the optical light curve is consistent with a contribution from an underlying Type Ic supernova like SN1998bw, or a dust echo, but such contributions cannot be securely established.Comment: 9 pages, 5 figures, LaTeX using A&A Document Class v4.05, to appear in A&

On the General Kerr/CFT Correspondence in Arbitrary Dimensions

We study conformal symmetries on the horizon of a general stationary and axisymmetric black hole. We find that there exist physically reasonable boundary conditions that uniquely determine a set of symmetry generators, which form one copy of the Virasoro algebra. For extremal black holes, Cardy's formula reproduces exactly the Bekenstein-Hawking entropy.Comment: 17 page

Phase-field approach to heterogeneous nucleation

We consider the problem of heterogeneous nucleation and growth. The system is described by a phase field model in which the temperature is included through thermal noise. We show that this phase field approach is suitable to describe homogeneous as well as heterogeneous nucleation starting from several general hypotheses. Thus we can investigate the influence of grain boundaries, localized impurities, or any general kind of imperfections in a systematic way. We also put forward the applicability of our model to study other physical situations such as island formation, amorphous crystallization, or recrystallization.Comment: 8 pages including 7 figures. Accepted for publication in Physical Review

Multi-wavelength observations of afterglow of GRB 080319B and the modeling constraints

We present observations of the afterglow of GRB 080319B at optical, mm and radio frequencies from a few hours to 67 days after the burst. Present observations along with other published multi-wavelength data have been used to study the light-curves and spectral energy distributions of the burst afterglow. The nature of this brightest cosmic explosion has been explored based on the observed properties and it's comparison with the afterglow models. Our results show that the observed features of the afterglow fits equally good with the Inter Stellar Matter and the Stellar Wind density profiles of the circum-burst medium. In case of both density profiles, location of the maximum synchrotron frequency $\nu_m$ is below optical and the value of cooling break frequency $\nu_c$ is below $X-$rays, $\sim 10^{4}$s after the burst. Also, the derived value of the Lorentz factor at the time of naked eye brightness is $\sim 300$ with the corresponding blast wave size of $\sim 10^{18}$ cm. The numerical fit to the multi-wavelength afterglow data constraints the values of physical parameters and the emission mechanism of the burst.Comment: 8 Pages, 3 Figures, Accepted for publication to Astronomy and Astrophysics on 02/04/200

How acceptable are antiretrovirals for the prevention of sexually transmitted HIV? A review of research on the acceptability of oral pre-exposure prophylaxis and treatment as prevention

Recent research has demonstrated how antiretrovirals (ARVs) could be effective in the prevention of sexually transmitted HIV. We review research on the acceptability of oral pre-exposure prophylaxis (PrEP) and treatment as prevention (TasP) for HIV prevention amongst potential users. We consider with whom, where and in what context this research has been conducted, how acceptability has been approached, and what research gaps remain. Findings from 33 studies show a lack of TasP research, PrEP studies which have focused largely on men who have sex with men (MSM) in a US context, and varied measures of acceptability. In order to identify when, where and for whom PrEP and TasP would be most appropriate and effective, research is needed in five areas: acceptability of TasP to people living with HIV; motivation for PrEP use and adherence; current perceptions and management of risk; the impact of broader social and structural factors; and consistent definition and operationalisation of acceptability which moves beyond adherence

De novo assembly and functional annotation of Myrciaria dubia fruit transcriptome reveals multiple metabolic pathways for L-ascorbic acid biosynthesis

Background Myrciaria dubia is an Amazonian fruit shrub that produces numerous bioactive phytochemicals, but is best known by its high L-ascorbic acid (AsA) content in fruits. Pronounced variation in AsA content has been observed both within and among individuals, but the genetic factors responsible for this variation are largely unknown. The goals of this research, therefore, were to assemble, characterize, and annotate the fruit transcriptome of M. dubia in order to reconstruct metabolic pathways and determine if multiple pathways contribute to AsA biosynthesis. Results In total 24,551,882 high-quality sequence reads were de novo assembled into 70,048 unigenes (mean length = 1150 bp, N50 = 1775 bp). Assembled sequences were annotated using BLASTX against public databases such as TAIR, GR-protein, FB, MGI, RGD, ZFIN, SGN, WB, TIGR_CMR, and JCVI-CMR with 75.2 % of unigenes having annotations. Of the three core GO annotation categories, biological processes comprised 53.6 % of the total assigned annotations, whereas cellular components and molecular functions comprised 23.3 and 23.1 %, respectively. Based on the KEGG pathway assignment of the functionally annotated transcripts, five metabolic pathways for AsA biosynthesis were identified: animal-like pathway, myo-inositol pathway, L-gulose pathway, D-mannose/L-galactose pathway, and uronic acid pathway. All transcripts coding enzymes involved in the ascorbate-glutathione cycle were also identified. Finally, we used the assembly to identified 6314 genic microsatellites and 23,481 high quality SNPs. Conclusions This study describes the first next-generation sequencing effort and transcriptome annotation of a non-model Amazonian plant that is relevant for AsA production and other bioactive phytochemicals. Genes encoding key enzymes were successfully identified and metabolic pathways involved in biosynthesis of AsA, anthocyanins, and other metabolic pathways have been reconstructed. The identification of these genes and pathways is in agreement with the empirically observed capability of M. dubia to synthesize and accumulate AsA and other important molecules, and adds to our current knowledge of the molecular biology and biochemistry of their production in plants. By providing insights into the mechanisms underpinning these metabolic processes, these results can be used to direct efforts to genetically manipulate this organism in order to enhance the production of these bioactive phytochemicals. The accumulation of AsA precursor and discovery of genes associated with their biosynthesis and metabolism in M. dubia is intriguing and worthy of further investigation. The sequences and pathways produced here present the genetic framework required for further studies. Quantitative transcriptomics in concert with studies of the genome, proteome, and metabolome under conditions that stimulate production and accumulation of AsA and their precursors are needed to provide a more comprehensive view of how these pathways for AsA metabolism are regulated and linked in this species