Modern requirements to preparation of professional and pedagogical personnel

The relevance of the researched problem is caused by the need of providing the educational organizations of the system of professional education with specialists in projecting and implementation of educational programs of training of workers and specialists of a middle link, and formation of a personnel resource for innovative productions. The purpose of the presented article consists in consideration of the discrepancies arising while introduction of the professional standard "Teacher of Vocational Training, Vocational Education and Further Vocational Education" and changes revelation of a pedagogical personnel which are necessary for improvement of preparation quality. The leading approach to the research of this problem is the system-based approach which allows us to reveal the discrepancies arising by the comparison of the professional standard and educational programs that are developed taking into account requirements of the educational standard for the direction of preparation Vocational Training (branch-wise). According to the analysis results, the option of adjustment of educational programs for the direction of preparation Vocational Training (branch-wise) is offered; it promotes improvement of preparation quality of pedagogical personnel for the system of professional education and further vocational education, and also the corporate educational organizations and innovative productions. The materials of the publication can be useful to heads of educational organizations while forming the personnel potential in educational institutions. © 2016 Zyryanova et al

Effect of intake of Vitrum vitamin-mineral complex in combination with adaptogens on immune status and physical work capacity in highly skilled student athletes

Purpose of the study: to study the effect of Vitrum in combination with adaptogens on immunological reactivity and physical performance in athletes under conditions of intense training.Materials and methods: 4 groups of athletes, 10 people in each, went through a cycle of intensive training according to a specially developed methodology. Group I took Vitrum, group II — Vitrum + Eleutherococcus extract, group III — Vitrum + ginseng tincture. Group IV — ascorbic acid and was used as a control. Blood for analysis was taken from the cubital vein before and after 28 days of drug administration. Indicators of immunological reactivity were determined by modern laboratory methods. Physical performance was determined by the index of the Harvard step test (IHST) and the PWC170 test.Results: the use of Vitrum in combination with adaptogens promotes a significant increase in the parameters of humoral (increase in IgA by 32–40 %, IgM by 28–43 %, IgG by 9–14 %) and cellular (increasing the completeness of phagocytosis by 9–37 % immunity.Conclusion: intake of Vitrum in combination with adaptogens was accompanied by a significant increase in nonspecific immunity and increased the level of fitness

An amperometric biosensor based on laccase immobilized in polymer matrices for determining phenolic compounds

An amperometric enzyme electrode based on laccase for determining phenolic compounds is proposed. The following three types of polymer materials were used for enzyme immobilization on the surface of a glassy-carbon electrode: positively charged cetyl ethyl poly (ethyleneimine) (CEPEI) and negatively charged commercial Nafion and Eastman AQ 29D polymers. The advantages and disadvantages of each of the above polymers for enzyme immobilization are discussed. The detection limits of the model phenolic compounds hydroquinone and pyrocatechol in a buffer solution on laccase immobilization in a Nation membrane were 3.5 x 10(-8) and 5.0 x 10(-8) M, respectively, at a signal-to-noise ratio of 3. Electrodes with laccase immobilized in Nation and Eastman AQ 29D membranes exhibited the shortest response time. The operating stability and the stability in storage can be significantly improved by the additional incorporation of gelatin in the polymer matrices. Gelatin prevents enzyme inactivation as a result of enzyme modification by the free-radical oxidation products of phenolic compounds

Pain as one of the risk factors for progression of knee osteoarthrosis

Objective: to study the impact of pain intensity on the progression of knee osteoarthrosis (OA). Subjects and methods. One hundred and ten patients with knee OA were examined at a 5-year interval. All the patients underwent a questionnaire survey and knee joint pain assessment using a visual analog scale (VAS) and standard radiography. Results. After 5-year follow-up, radiographic OA progression was seen in 40 patients (Group 2); its stage remained the same in 70 patients (Group 1). In both groups, the patients were matched for age (59.2+9.5 and 59.0+8.1 years) and disease duration (11.1+10.6 and 13.7+9.9 years). During the first examination, pain on walking was more severe in Group 1 than in Group 2: 57.8+16.6 and 48.7+13.3 mm by VAS (р=0.002), as well as severe joint pain was predominant in these patients: 22.5 and 11.4%, respectively. Over the 5-year period, there was an increase in pain intensity. At the end of the follow-up, the patients with progressive OA rated their knee joint pain as severe in 35% of cases whereas in this index the non-progression group was only 12.9 (p = 0.012). Conclusion. In the OA progression group, pain intensity was initially statistically higher than that in the non-progression group. During 5-year follow-up, Group 1 showed an increase in knee joint pain intensity on walking, which can be considered as one of the predictors of gonarthrosis progression

To the History of the Development of Molecular-Genetic Technologies in the Urals

The authors analyze the state of molecular genetic technologies in Russia and the Urals. They reveal the concept of “molecular genetic technologies”, identify the main directions of research and the use of appropriate technologies. They prove that the processes of development of this type of technology are possible only in the presence of institutional conditions.Авторы анализируют состояние молекулярно-генетических технологий в России и на Урале. Раскрывают понятие «молекулярно-генетические технологии», выявляют основные направления исследований и использования соответствующих технологий. Доказывают, что процессы развития этого вида технологий возможны только при наличии институциональных условий.Статья подготовлена при поддержке гранта РФФИ № 18-29-14059 «Правовые и этические аспекты технологий геномных исследований при врожденных ошибках иммунитета»

ГИРОТРОНЫ С КОНУСООБРАЗНЫМИ РЕЗОНАТОРАМИ

In this paper we compared the efficiency of the cylindrical, conical, and biconical types of gyrotron resonators. Based on the results of comparing the three studied variants of gyrotron profile, it was concluded that the regular-type profile is the least efficient. This type of a resonator made it possible to achieve the level of efficiency of only 23 %, which can be increased in the regular-waveguide gyrothrons only through several modes or by recovering the electrons on the collector. The medium efficiency option is the biconical profile of the resonator. Its efficiency accounted for 42 %. Through a scientific study we revealed an increase in the efficiency for gyrotrons with conical resonators from 23 to 50 % in the TE01 wave. It is worth mentioning that obtaining such efficiency requires phase grouping of the electrons in an increasing high-frequency field by means of an electromagnetic field with further selection of energy from the electron beam in a strong decaying electromagnetic field. The efficiency of 50 % exceeds significantly that of a gyrotron with a regular cavity profile of ~30 %. The gyrotron efficiency for a waveguide profile with a conical resonator and with recovery on the collector can reach 80 %. To carry out the calculations, the KEDR software package was used, and the optimization of the gyrotron parameters, in particular, was carried out using the GYRO-K software. This software has several advantages over other similar options based on the “PIC” code. GYRO-K makes it possible to obtain a high convergence rate when solving boundary value problems, as well as to solve the problem of optimizing the waveguide profile of gyroresonance devices with an acceptable computational burden. Conical cavity gyrotrons can be widely used in industry to create effective gyrotrons for spectroscopy, diagnostics of various media, and for technological needs.Произведено сравнение эффективности цилиндрического, конического и биконического типов резонаторов гиротронов. По результатам сравнения трех исследуемых вариантов профиля гиротрона были сделаны выводы, что наименее эффективным является профиль регулярного типа. Данный вид резонатора позволил достичь уровня коэффициента полезного действия лишь в 23 %. Повысить коэффициент полезного действия в гиротронах с регулярным волноводом можно только за счет наличия нескольких мод или с помощью рекуперациии электронов на коллекторе. Средним вариантом по коэффициенту полезного действия является биконический профиль резонатора. Его коэффициент полезного действия получился равным 42 %. При проведении научного исследования было выявлено увеличение коэффициента полезного действия для гиротронов с коническими резонаторами с 23 до 50 % на волне TE01. Стоит отметить, что для получения такого коэффициента полезного действия необходимо производить группировку электронов по фазе в нарастающем высокочастотном поле посредством электромагнитного поля с дальнейшим отбором энергии у электронного потока в сильном спадающем электромагнитном поле. Коэффициент полезного действия, достигающий 50 %, существенно выше коэффициента полезного действия гиротрона с регулярным профилем резонатора ~30 %. Коэффициент полезного действия гиротрона для профиля волновода с коническим резонатором и с рекуперацией на коллекторе может достигать уровня в 80 %. Для проведения расчетов использовался программный комплекс КЕДР, в частности, оптимизация параметров гиротронов проводилась с помощью программного продукта GYRO-K. Данный программный продукт имеет ряд преимуществ перед другими подобными программными продуктами, основанных на «PIC» коде. GYRO-K позволяет получить высокую скорость сходимости при решении краевых задач, а также решать задачу оптимизации профиля волновода гирорезонансных приборов за приемлемое время счета. Гиротроны на коническом резонаторе могут найти широкое применение в промышленности при создании эффективных гиротронов для спектроскопии, диагностики различных сред и для технологических нужд

Pathophysiological aspects of the interaction of hypoxia and physical load (experimental study)

Background. A typical extreme factor is hypoxia, which significantly affects the ability to perform tasks of professional activity, in particular, physical activity of various intensity. Physical and neuro-emotional stress under conditions of hypoxia can cause the breakdown of compensatory and adaptive mechanisms with the development of extreme and critical conditions.The aim of the study. Quantitative assessment of the effect of hypoxia on the physical performance of laboratory animals at different levels of their natural resistance.Research methods. Modeling of hypoxia was carried out by the methods of pressure chamber rise of laboratory animals and the administration of a methemoglobin former. Resistance to hypoxic hypoxia was assessed by the criterion of the threshold elevation, to hemic hypoxia – by the lifetime. The criterion for the functional state of laboratory animals was their ability to perform physical activity, which was created by treadmill run or swimming with a load.Results of the study. An altitude of 3500 m causes a decrease in the physical performance of male rats by a third of normoxia level; an altitude of 5000 m – decrease by two times; an altitude of 6500 m – decrease by four times, and at an altitude of 8000 m makes running physical activity by rats impossible. A mild degree of hemic hypoxia causes a decrease in swimming time by almost 2 times, which corresponds to the effect of an altitude of 5000 m on the working capacity; and a moderate degree of hemic hypoxia causes a decrease in swimming time by more than 3 times, which approximately corresponds to the effect of an altitude of 6000 m. The level of resistance to hypoxia has a moderate effect on physical performance, and the factor of a low level of physical performance has almost no effect on the resistance to hypoxia (28 % and 7 % of the trait variability respectively). In animals with an initially low level of resistance to hypoxia, there is also a decrease in working capacity by an average of 20 %.Conclusion. Hemic hypoxia is an adequate model in studies with a complex effect of various extreme factors. Simulation of mild hypoxia caused by the administration of 30 mg/kg of sodium nitrite is an optimal method

NRF2-driven miR-125B1 and miR-29B1 transcriptional regulation controls a novel anti-apoptotic miRNA regulatory network for AML survival

Transcription factor NRF2 is an important regulator of oxidative stress. It is involved in cancer progression, and has abnormal constitutive expression in acute myeloid leukaemia (AML). Posttranscriptional regulation by microRNAs (miRNAs) can affect the malignant phenotype of AML cells. In this study, we identified and characterised NRF2-regulated miRNAs in AML. An miRNA array identified miRNA expression level changes in response to NRF2 knockdown in AML cells. Further analysis of miRNAs concomitantly regulated by knockdown of the NRF2 inhibitor KEAP1 revealed the major candidate NRF2-mediated miRNAs in AML. We identified miR-125B to be upregulated and miR-29B to be downregulated by NRF2 in AML. Subsequent bioinformatic analysis identified putative NRF2 binding sites upstream of the miR-125B1 coding region and downstream of the mir-29B1 coding region. Chromatin immunoprecipitation analyses showed that NRF2 binds to these antioxidant response elements (AREs) located in the 5′ untranslated regions of miR-125B and miR-29B. Finally, primary AML samples transfected with anti-miR-125B antagomiR or miR-29B mimic showed increased cell death responsiveness either alone or co-treated with standard AML chemotherapy. In summary, we find that NRF2 regulation of miR-125B and miR-29B acts to promote leukaemic cell survival, and their manipulation enhances AML responsiveness towards cytotoxic chemotherapeutics