Dry granular flows: micromechanical interpretation of impacts on rigid obstacles.

The evaluation of impact forces exerted by flowing granular masses on rigid obstacles is of fundamental importance for the assessment of the associated risk and for the design of protection measures. A number of formulae are available in the literature for the maximum impact force; most of them are based on oversimplifying hypotheses about the behaviour of the granular material. For practical applications, formulations based on either hydrodynamic or elastic body models are often employed. These formulations require the use of empirical correcting factors. In order to better understand the impact mechanics, the authors have recently performed an extensive numerical campaign by using a Discrete Element approach (PFC3D code), where a dry granular mass is represented as a random distribution of rigid spherical particles. A new design formula, combining the hydrodynamic and elastic body theories, has been proposed on the base of the results obtained at the macroscopic scale. The parameters of the formula have been correlated with geometrical factors, namely front inclination and flow height. In this paper, the same DEM model is further used in order to investigate the relationship between the evolution with time of the impact force and the micromechanics of the granular mass. In particular, information about contact forces and particle velocities will be discussed and critically compared with macroscopic results. In order to progressively introduce the complexity of the impact phenomenon, three geometrical conditions are considered: a) vertical front, confined flow; b) vertical front, free surface flow; c) inclined front, free surface flow

A meteorological–hydrological regional ensemble forecast for an early-warning system over small Apennine catchments in Central Italy

Abstract. The weather forecasts for precipitation have considerably improved in recent years thanks to the increase of computational power. This allows for the use of both a higher spatial resolution and the parameterization schemes specifically developed for representing sub-grid scale physical processes at high resolution. However, precipitation estimation is still affected by errors that can impact the response of hydrological models. To the aim of improving the hydrological forecast and the characterization of related uncertainties, a regional-scale meteorological–hydrological ensemble is presented. The uncertainties in the precipitation forecast and how they propagate in the hydrological model are also investigated. A meteorological–hydrological offline coupled ensemble is built to forecast events in a complex-orography terrain where catchments of different sizes are present. The Best Discharge-based Drainage (BDD; both deterministic and probabilistic) index, is defined with the aim of forecasting hydrological-stress conditions and related uncertainty. In this context, the meteorological–hydrological ensemble forecast is implemented and tested for a severe hydrological event which occurred over Central Italy on 15 November 2017, when a flood hit the Abruzzo region with precipitation reaching 200 mm (24 h)−1 and producing damages with a high impact on social and economic activities. The newly developed meteorological–hydrological ensemble is compared with a high-resolution deterministic forecast and with the observations (rain gauges and radar data) over the same area. The receiver operating characteristic (ROC) statistical indicator shows how skilful the ensemble precipitation forecast is with respect to both rain-gauge- and radar-retrieved precipitation. Moreover, both the deterministic and probabilistic configurations of the BDD index are compared with the alert map issued by Civil Protection Department for the event showing a very good agreement. Finally, the meteorological–hydrological ensemble allows for an estimation of both the predictability of the event a few days in advance and the uncertainty of the flood. Although the modelling framework is implemented on the basins of the Abruzzo region, it is portable and applicable to other areas

High-Throughput Microfluidic Platform for 3D Cultures of Mesenchymal Stem Cells, Towards Engineering Developmental Processes

The development of in vitro models to screen the effect of different concentrations, combinations and temporal sequences of morpho-regulatory factors on stem/progenitor cells is crucial to investigate and possibly recapitulate developmental processes with adult cells. Here, we designed and validated a microfluidic platform to (i) allow cellular condensation, (ii) culture 3D micromasses of human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) under continuous flow perfusion, and (ii) deliver defined concentrations of morphogens to specific culture units. Condensation of hBM-MSCs was obtained within 3 hours, generating micromasses in uniform sizes (56.2 ± 3.9 μm). As compared to traditional macromass pellet cultures, exposure to morphogens involved in the first phases of embryonic limb development (i.e. Wnt and FGF pathways) yielded more uniform cell response throughout the 3D structures of perfused micromasses (PMMs), and a 34-fold higher percentage of proliferating cells at day 7. The use of a logarithmic serial dilution generator allowed to identify an unexpected concentration of TGFβ3 (0.1 ng/ml) permissive to hBM-MSCs proliferation and inductive to chondrogenesis. This proof-of-principle study supports the described microfluidic system as a tool to investigate processes involved in mesenchymal progenitor cells differentiation, towards a ‘developmental engineering’ approach for skeletal tissue regeneration

First Beam Based Aperture Measurements in the Arcs of the CERN Large Hadron Collider

The LHC injection tests performed in August and early September 2008 in preparation for the circulating beam operation provided the first opportunity to measure with beam the mechanical aperture in two LHC sectors (2-3 and 7- 8). The aperture was probed by exciting free oscillations and local orbit bumps of the injected beam trajectories. Intensities of a few 109 protons were used to remain safely below the quench limit of superconductingmagnets in case of beam losses. The methods used to measure the mechanical aperture, the available on-line tools, and beam measurements for both sectors are presented. Detailed comparisons with the expected results from the as-built aperture models are also presented. It is shown that the measurements results are in good agreement with the LHC design aperture

One-dimensional Models for Cell Feeding in Micro-channels.

Lab-on-Chip technology is an emerging tool to obtain, culture and study different kinds of tissue models. In particular, concerning cardiac microtissues, it could be exploited to investigate cellular behavior in response to biomimetic stimulation (e.g., electrical and/or mechanical actuations).Cell culture in microfluidic chip, usually last several days. During this period of time, cells need feeding to stay alive, proliferate and organize. Different configurations and sizes of microchip features can cause non-uniform distribution of nutrients and wastes on chip, which must be taken into account in studying the response of cells to electrical and/or mechanical stimulations. A simple one-dimensional model of diffusion was used to simulate the concentration of a nutrient and a waste in a previously developed microfluidic chip. For quasi-steady processes, analytical solutions were obtained and analyzed

PO-0857: GTV-based prescription and Monte Carlo treatment planning in Cyberknife treatments for lung lesions

n/

Beam halo dynamics and control with hollow electron beams

Experimental measurements of beam halo diffusion dynamics with collimator scans are reviewed. The concept of halo control with a hollow electron beam collimator, its demonstration at the Tevatron, and its possible applications at the LHC are discussed.Comment: 5 pages, 4 figures, in Proceedings of the 52nd ICFA Advanced Beam Dynamics Workshop on High-Intensity and High-Brightness Hadron Beams (HB2012), Beijing, China, 17-21 September 201

Nuclear spin ratios of deuterated ammonia in prestellar cores. LAsMA observations of H-MM1 and Oph D

We determine the ortho/para ratios of NH2D and NHD2 in two dense, starless cores, where their formation is supposed to be dominated by gas-phase reactions, which, in turn, is predicted to result in deviations from the statistical spin ratios. The Large APEX sub-Millimeter Array (LAsMA) multibeam receiver of the Atacama Pathfinder EXperiment (APEX) telescope was used to observe the prestellar cores H-MM1 and Oph D in Ophiuchus in the ground-state lines of ortho and para NH2D and NHD2. The fractional abundances of these molecules were derived employing 3D radiative transfer modelling, using different assumptions about the abundance profiles as functions of density. We also ran gas-grain chemistry models with different scenarios concerning proton or deuteron exchanges and chemical desorption from grains to find out if one of these models can reproduce the observed spin ratios. The observationally deduced ortho/para ratios of NH2D and NHD2 are in both cores within 10% of their statistical values 3 and 2, respectively, and taking 3-sigma limits, deviations from these of about 20% are allowed. Of the chemistry models tested here, the model that assumes proton hop (as opposed to full scrambling) in reactions contributing to ammonia formation, and a constant efficiency of chemical desorption, comes nearest to the observed abundances and spin ratios. The nuclear spin ratios derived here are in contrast with spin-state chemistry models that assume full scrambling in proton donation and hydrogen abstraction reactions leading to deuterated ammonia. The efficiency of chemical desorption influences strongly the predicted abundances of NH3, NH2D, and NHD2, but has a lesser effect on their ortho/para ratios. For these the proton exchange scenario in the gas is decisive. We suggest that this is because of rapid re-processing of ammonia and related cations by gas-phase ion-molecule reactions.Comment: accepted for publication in Astronomy & Astrophysic

The Green Bank Ammonia Survey: Unveiling the Dynamics of the Barnard 59 star-forming Clump

Understanding the early stages of star formation is a research field of ongoing development, both theoretically and observationally. In this context, molecular data have been continuously providing observational constraints on the gas dynamics at different excitation conditions and depths in the sources. We have investigated the Barnard 59 core, the only active site of star formation in the Pipe Nebula, to achieve a comprehensive view of the kinematic properties of the source. These information were derived by simultaneously fitting ammonia inversion transition lines (1,1) and (2,2). Our analysis unveils the imprint of protostellar feedback, such as increasing line widths, temperature and turbulent motions in our molecular data. Combined with complementary observations of dust thermal emission, we estimate that the core is gravitationally bound following a virial analysis. If the core is not contracting, another source of internal pressure, most likely the magnetic field, is supporting it against gravitational collapse and limits its star formation efficiency.Comment: 18 pages, 18 figure

Biological effects of EF24, a curcumin derivative, alone or combined with mitotane in adrenocortical tumor cell lines

Background: Curcumin has numerous properties and is used in many preclinical conditions, including cancer. It has low bioavailability, while its derivative EF24 shows enhanced solubility. However, its effects have never been explored in adrenocortical tumor cell models. The efficacy of EF24 alone or combined with mitotane (reference drug for adrenocortical cancer) was evaluated in two adrenocortical tumor cell lines, SW13 and H295R. Method and Results: EF24 reduced cell viability with an IC50 (half maximal inhibitory concentration) of 6.5 \ub1 2.4 \ub5M and 4.9 \ub1 2.8 \ub5M for SW13 and H295R cells, respectively. Combination index (EF24 associated with mitotane) suggested an additivity effect in both cell lines. Cell cycle analysis revealed an increase in subG0/G1 phase, while motility assay showed a decrease in migratory cell capacity, and similarly, clonogenic assay indicated that EF24 could reduce colony numbers. Furthermore, Wnt/\u3b2-catenin, NF-\u3baB, MAPK, and PI3k/Akt pathways were modulated by Western blot analysis when treating cells with EF24 alone or combined with mitotane. In addition, intracellular reactive oxygen species levels increased in both cell lines. Conclusion: This work analyzed EF24 in adrenocortical tumor cell lines for the first time. These results suggest that EF24 could potentially impact on adrenocortical tumors, laying the foundation for further research in animal models