60 research outputs found
ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΡΠΎΡΠΈΠ»Ρ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² Π³ΠΈΠΏΠΎΠ³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΠ±ΠΎΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π²ΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ Ρ ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ
Objectives. Herbal hypoglycemic drugs complement the conventional approach to the treatment of type-2 diabetes based on the use of synthetic prescription drugs. However, their scientifically based application and standardization are limited due to inadequate and often outdated information on their chemical composition. Accordingly, we have developed a hypoglycemic collection (HGΠ‘) consisting of common bean pods (Phaseolus vulgaris L.), bilberry shoots (Vaccinium myrtillus L.), galega herb (Gallega officinalis L.), common knotgrass herb (Polygonum aviculare L.), burdock roots (Arctium lappa L.), and cinnamon rose hips (Rosa cinnamomea L.). According to a number of researchers, the antidiabetic properties of these herbs are largely due to the presence of polyphenolic compounds, especially flavonoids. The aim of this study was to determine the profile of flavonoids in the HGΠ‘ and in its total dry extract (TDE).Methods. The study was performed by reverse-phase high-performance liquid chromatography with diode array and mass spectrometric detection.Results. Nine individual flavonol glycosidesβderivatives of myricetin, quercetin, kaempferol and kaempferideβwere identified in the HGC and the TDE. The main flavonol glycosides in the studied objects were robinin and kaempferol-3-glucuronide, the contents of which in the HGC were 2.09 and 2.22 mg/g, in the TDE 4.85 and 3.84 mg/g, respectively. The other flavonol glycosides were determined in the HGC and its TDE at significantly lower concentrations.Conclusions. The method developed in the study can be used to standardize HGCs and estimate their pharmacological activities.Π¦Π΅Π»ΠΈ. ΠΠΈΠΏΠΎΠ³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ ΡΡΠΏΠ΅ΡΠ½ΠΎ Π΄ΠΎΠΏΠΎΠ»Π½ΡΡΡ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ΅ΡΠ΅ΠΏΡΡΡΠ½ΡΠ΅ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π°, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΠΈΠ΅ΡΡ Π² ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Π΅ ΠΊ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠ°Ρ
Π°ΡΠ½ΠΎΠ³ΠΎ Π΄ΠΈΠ°Π±Π΅ΡΠ° 2 ΡΠΈΠΏΠ°. ΠΠ΄Π½Π°ΠΊΠΎ Π½Π°ΡΡΠ½ΠΎ ΠΎΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΠΎΠ΅ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΠΈΡ ΡΠ°ΠΊΠΈΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½Ρ ΠΈΠ·-Π·Π° Π½Π΅Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½ΠΎΠΉ ΠΈ ΡΠ°ΡΡΠΎ ΡΡΡΠ°ΡΠ΅Π²ΡΠ΅ΠΉ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΎΠ± ΠΈΡ
Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΠΎΡΡΠ°Π²Π΅. ΠΠ°ΠΌΠΈ Π±ΡΠ» ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½ Π³ΠΈΠΏΠΎΠ³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ±ΠΎΡ (ΠΠΠ‘), ΡΠΎΡΡΠΎΡΡΠΈΠΉ ΠΈΠ· ΡΡΠ²ΠΎΡΠΎΠΊ ΡΠ°ΡΠΎΠ»ΠΈ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ (Phaseolus vulgaris L.), ΠΏΠΎΠ±Π΅Π³ΠΎΠ² ΡΠ΅ΡΠ½ΠΈΠΊΠΈ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ (Vaccinium myrtillus L.), ΡΡΠ°Π²Ρ Π³Π°Π»Π΅Π³ΠΈ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ (Gallega officinalis L.), ΡΡΠ°Π²Ρ Π³ΠΎΡΡΠ° ΠΏΡΠΈΡΡΠ΅Π³ΠΎ (ΡΠΏΠΎΡΡΡΠ°) (Polygonum aviculare L.), ΠΊΠΎΡΠ½Π΅ΠΉ Π»ΠΎΠΏΡΡ
Π° Π±ΠΎΠ»ΡΡΠΎΠ³ΠΎ (Arctium lappa L.), ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΡΠΈΠΏΠΎΠ²Π½ΠΈΠΊΠ° ΠΊΠΎΡΠΈΡΠ½ΠΎΠ³ΠΎ (Rosa cinnamomea L.). ΠΠΎ ΠΌΠ½Π΅Π½ΠΈΡ ΡΡΠ΄Π° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»Π΅ΠΉ, Π°Π½ΡΠΈΠ΄ΠΈΠ°Π±Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° Π²ΡΡΠ΅ΡΠΏΠΎΠΌΡΠ½ΡΡΡΡ
ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΎΠΌ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Ρ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ΠΌ Π² Π½ΠΈΡ
ΠΏΠΎΠ»ΠΈΡΠ΅Π½ΠΎΠ»ΡΠ½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ². Π¦Π΅Π»Ρ Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΡΠΎΡΠΈΠ»Ρ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² ΠΠΠ‘ ΠΈ Π² ΡΡΠΌΠΌΠ°ΡΠ½ΠΎΠΌ ΡΡΡ
ΠΎΠΌ ΡΠΊΡΡΡΠ°ΠΊΡΠ΅ (Π‘Π‘Π) Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΠΠ‘.ΠΠ΅ΡΠΎΠ΄Ρ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΎΠ±ΡΠ°ΡΠ΅Π½ΠΎ-ΡΠ°Π·ΠΎΠ²ΠΎΠΉ Π²ΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ Ρ Π΄ΠΈΠΎΠ΄Π½ΠΎ-ΠΌΠ°ΡΡΠΈΡΠ½ΡΠΌ ΠΈ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π ΠΠΠ‘ ΠΈ Π‘Π‘Π Π±ΡΠ»ΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ Π΄Π΅Π²ΡΡΡ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ
ΡΠ»Π°Π²ΠΎΠ½ΠΎΠ»Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ΄ΠΎΠ² β ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
ΠΌΠΈΡΠΈΡΠ΅ΡΠΈΠ½Π°, ΠΊΠ²Π΅ΡΡΠ΅ΡΠΈΠ½Π°, ΠΊΠ΅ΠΌΠΏΡΠ΅ΡΠΎΠ»Π° ΠΈ ΠΊΠ΅ΠΌΠΏΡΠ΅ΡΠΈΠ΄Π°. ΠΡΠ½ΠΎΠ²Π½ΡΠΌΠΈ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠ»Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ΄Π°ΠΌΠΈ Π² ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
ΠΎΠ±ΡΠ΅ΠΊΡΠ°Ρ
Π±ΡΠ»ΠΈ ΡΠΎΠ±ΠΈΠ½ΠΈΠ½ ΠΈ ΠΊΠ΅ΠΌΠΏΡΠ΅ΡΠΎΠ»-3-Π³Π»ΡΠΊΡΡΠΎΠ½ΠΈΠ΄, ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ
Π² ΠΠΠ‘ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΎ 2.09 ΠΈ 2.22 ΠΌΠ³/Π³, Π² Π‘Π‘Π β 4.85 ΠΈ 3.84 ΠΌΠ³/Π³, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΡΡΠ°Π»ΡΠ½ΡΠ΅ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠ»Π³Π»ΠΈΠΊΠΎΠ·ΠΈΠ΄Ρ Π±ΡΠ»ΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ Π² ΠΠΠ‘ ΠΈ Π‘Π‘Π Π² ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Π±ΠΎΠ»Π΅Π΅ Π½ΠΈΠ·ΠΊΠΈΡ
ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡΡ
.ΠΡΠ²ΠΎΠ΄Ρ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠ°Π±ΠΎΡΡ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΏΡΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΠΈΠΈ ΠΠΠ‘ ΠΈ ΠΎΡΠ΅Π½ΠΊΠ΅ Π΅Π³ΠΎ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ
A study of a complex of bioactive compounds in the fruits of promising blue honeysuckle (<i>Lonicera caerulea</i> L.) cultivars
Background. Blue honeysuckle (Lonicera caerulea L.) is a valuable source of bioactive compounds (BAC) of polyphenolic nature and rare for horticultural berries iridoids, which have antioxidant, anti-inflammatory, antimicrobial and other properties and are widely used in the food, medical and cosmetic industries.Materials and methods. The berries of 20 released honeysuckle cultivars of Russian, Canadian and U.S. origin, reproduced at the I.V. Michurin Federal Science Center in Tambov Province, were studied for the content of the main BAC groups using modern methods (spectrophotometry, HPLC-UV, HPLC-RID, and HPLC-DAD-MS).Results and discussion. The main BAC groups (the content and profile of anthocyanins, proanthocyanidins, flavonols and flavones, hydroxycinnamic acids (HCA), iridoids, and organic acids) as well as mono- and disaccharides were studied in detail. A comparative study of the biological value of domestic and foreign honeysuckle cultivars was carried out.Conclusions. The study resulted in identifying the most promising cultivars of honeysuckle according to the content of the main BAC groups
Know Your Heart: Rationale, design and conduct of a cross-sectional study of cardiovascular structure, function and risk factors in 4500 men and women aged 35-69 years from two Russian cities, 2015-18
Russia has one of the highest rates of cardiovascular disease in the world. The International Project on Cardiovascular Disease in Russia (IPCDR) was set up to understand the reasons for this. A substantial component of this study was the Know Your Heart Study devoted to characterising the nature and causes of cardiovascular disease in Russia by conducting large cross-sectional surveys in two Russian cities Novosibirsk and Arkhangelsk. The study population was 4542 men and women aged 35-69 years recruited from the general population. Fieldwork took place between 2015-18. There were two study components: 1) a baseline interview to collect information on socio-demographic characteristics and cardiovascular risk factors, usually conducted at home, and 2) a comprehensive health check at a primary care clinic which included detailed examination of the cardiovascular system. In this paper we describe in detail the rationale for, design and conduct of these studies.The International Project on Cardiovascular Disease in Russia (IPCDR) project was supported in part by a Wellcome Trust Strategic Award [100217].
The project was also funded by the Arctic University of Norway, UiT in TromsΓΈ; Norwegian Institute of Public Health; the Norwegian Ministry of Health and Social Affairs
ΠΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π»ΡΡΠ΅Π²ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΠΏΡΠΈ ΠΏΡΠΎΡΠΈΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°ΡΡΡΠΈΡΠ΅
The comparative evaluation of modern imaging methods gives evidence of great opportunities of both MRT and US in the detection of structural alterations of joints at psoriatic arthritis. The key role is assigned to ultrasonography which has proved to be highly sensitive in the disclosure of peripheral joint synovitis and allows the follow-up during the treatment course.Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΠΎΡΠ΅Π½ΠΊΠ° ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π²ΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ ΠΎ Π±ΠΎΠ»ΡΡΠΈΡ
Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡΡ
ΠΊΠ°ΠΊ ΠΌΠ°Π³Π½ΠΈΡΠ½ΠΎΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ½ΠΎΠΉ ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ, ΡΠ°ΠΊ ΠΈ ΡΠ»ΡΡΡΠ°Π·Π²ΡΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π² ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΠΈ ΡΡΡΡΠΊΡΡΡΠ½ΡΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΡΡΡΡΠ°Π²ΠΎΠ² ΠΏΡΠΈ ΠΏΡΠΎΡΠΈΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°ΡΡΡΠΈΡΠ΅. ΠΡΠΎΠ±Π°Ρ ΡΠΎΠ»Ρ ΠΎΡΠ²ΠΎΠ΄ΠΈΡΡΡ ΡΠ»ΡΡΡΠ°Π·Π²ΡΠΊΠΎΠ²ΠΎΠΌΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ, ΠΊΠΎΡΠΎΡΠΎΠ΅ ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ Π² Π²ΡΡΠ²Π»Π΅Π½ΠΈΠΈ ΡΠΈΠ½ΠΎΠ²ΠΈΡΠΎΠ² Π² ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΡΡΠ°Π²Π°Ρ
, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΎΡΠ΅Π½ΠΈΠ²Π°ΡΡ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Π½Π° ΡΠΎΠ½Π΅ Π»Π΅ΡΠ΅Π½ΠΈΡ
Flavonoid-profile determination for a hypoglycemic collection by high-performance liquid chromatography
Objectives. Herbal hypoglycemic drugs complement the conventional approach to the treatment of type-2 diabetes based on the use of synthetic prescription drugs. However, their scientifically based application and standardization are limited due to inadequate and often outdated information on their chemical composition. Accordingly, we have developed a hypoglycemic collection (HGΠ‘) consisting of common bean pods (Phaseolus vulgaris L.), bilberry shoots (Vaccinium myrtillus L.), galega herb (Gallega officinalis L.), common knotgrass herb (Polygonum aviculare L.), burdock roots (Arctium lappa L.), and cinnamon rose hips (Rosa cinnamomea L.). According to a number of researchers, the antidiabetic properties of these herbs are largely due to the presence of polyphenolic compounds, especially flavonoids. The aim of this study was to determine the profile of flavonoids in the HGΠ‘ and in its total dry extract (TDE).Methods. The study was performed by reverse-phase high-performance liquid chromatography with diode array and mass spectrometric detection.Results. Nine individual flavonol glycosidesβderivatives of myricetin, quercetin, kaempferol and kaempferideβwere identified in the HGC and the TDE. The main flavonol glycosides in the studied objects were robinin and kaempferol-3-glucuronide, the contents of which in the HGC were 2.09 and 2.22 mg/g, in the TDE 4.85 and 3.84 mg/g, respectively. The other flavonol glycosides were determined in the HGC and its TDE at significantly lower concentrations.Conclusions. The method developed in the study can be used to standardize HGCs and estimate their pharmacological activities
USE OF SIBUTRAMIN IN PHARMACEUTICAL DRUGS AND ANOREXIC DIETARY SUPPLEMENTS (REVIEW)
Background. The most effective anorexic drugs on the pharmaceutical market are sibutramine. According to the standard scheme, sibutramine is similar to amphetamine. The active metabolites of sibutramine, formed in the process of its metabolism, have high pharmacological activity compared with the original substances, so the drug may be toxic to the body. Sibutramine is used in the complex treatment of obesity, when adjusting excess body weight in type II diabetes, and also used in polycystic ovary syndrome. The drug has many serious undesirable side effects, such as an increase in systolic and / or diastolic pressure and heart rate; headaches, insomnia, disorders of the gastrointestinal tract and can cause death. In many countries of the world a wide range of serious side effects has been banned.Text. A description of the drug sibutramine, its status on the pharmaceutical market of the Russian Federation and other countries of the world, an overview of possible methods for determining sibutramine in food supplements.Conclusion. Recently, information about the undeclared addition of sibutramine to the anorexigenic dietary supplement (dietary supplement) to increase their effectiveness has increasingly appeared. Therefore, it is necessary to develop methods for determining sibutramine in anorexic dietary supplements. In this regard, an analysis of the methods used for the quantitative determination of sibutramine. As a rule, spectral methods are used to determine the authenticity of a sibutramine substance. The most common and effective method for determining authenticity and quantitative analysis of sibutramine is the HPLC (high performance liquid chromatography) method using mass spectrometric and diode array detectors (DAD). At the moment, a method for determining sibutramine in the composition of multicomponent drugs and dietary supplements to food of anorexigenic action is being developed, which will contribute to the development of pharmacy
STROMAL CELLS OF FAT TISSUE FOR REGENERATIVE SURGERY
Background. Generalize modern data on the current opportunities and the perspective application directions of stem/stromal cells of fat tissue in regenerative surgery.Results. Preclinical and clinical developments of cellular transplantology techniques and tissue engineering with use of the cultivated multipotent mesenchymal stromal cells of fat tissue and freshly isolated stromal vascular fraction of fat tissue in plastic surgery, maxillofacial surgery, orthopedics, neurosurgery, coloproctology, urology, thoracic surgery, cardiac surgery are presented.Conclusion. Transplantation of stromal vascular fraction of fat tissue for which releasing we do not perform cultivation of cells out of a human body is a safe and effective regenerative technology with an application potential in the wide range of surgical areas
Study of Structure and Quality of Different Silicon Oxides Using FTIR and Raman Microscopy
In this work, SiO2 and fluorine and phosphorous doped SiO2 thin films are investigated using FTIR and Raman techniques. FTIR spectroscopy was performed at normal and oblique incidence of the probe beam in transmission and reflection modes. The effect of polarisation and angle of incidence of the probe beam is examined for the case of reflection mode. Infrared spectra taken from doped oxides show that the structure changes with the passage of time. Alternate methods to calculate the thickness of the doped film are therefore discussed. Infrared spectra of electron beam evaporated oxides give valuable information on their structure and water content. The porosity is calculated for these samples. Finally, micro-Raman spectroscopy is used to measure the fluorine content in a device structure
High concentration of H<sub>2</sub> and O<sub>2</sub> nanobubbles in water electrolytes and their collective optical effect
Water electrolysis with a fast change of the polarity pumps in the liquid a huge amount of hydrogen and oxygen gases. In contrast with the DC electrolysis the gases do not form visible bubbles but change significantly the refractive index of the liquid nearby the electrodes from n = 1.35 to the values smaller than 1.19. The decrease of n is registered as distortion of the images of the electrodes. We argue that all the gas is collected in H2 and O2 nanobubbles with a size smaller than 200 nm. The concentration of nanobubbles with a size of 100 nm is estimated as 1021 m-3. Due to a significant contribution from the Laplace pressure the effective supersaturation reaches 500 for hydrogen and 150 for oxygen
Development and Validation of Sibutramine Determination in Drug Products by Capillary Electrophoresis
Introduction. Recently, there has been a growing trend in the number of obese and overweight patients. To date, sibutramine is the most effective drug for treating obesity and overweight. The drug is an inhibitor of the reuptake of serotonin and norepinephrine, which leads to a decrease in hunger, and therefore, to weight loss.Aim. To develop and validate a methodology for the determination of sibutramine in drugs by capillary electrophoresis (CE) using an ultraviolet diode array detector.Materials and methods. Quantitative determination of sibutramine in drugs was carried out using the CE method with an ultraviolet diode array detector. A solution of phosphate buffer 50 mmol pH = 7.0 was used as a solvent and working electrolyte; to separate the peaks β quartz capillary 56 cm, 50 ΞΌm.Results and discussion. The developed method was validated according to the following parameters: specificity, linearity, correctness, precision, limit of detection and limit of quantification.Conclusion. A method for the quantitative determination of sibutramine in drugs by the CE method using an ultraviolet diode array detector has been developed and validated. This method meets all the requirements of General Pharmacopoeia Monograph 1.1.0012.15 Β«Validation of the analytical methodΒ» and can be used to control the quality of drugs, the active pharmaceutical substance of which is sibutramine
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