70 research outputs found
Carcass Compound Materials Base on Fluoropolymer for Tissue Engineering in Orthopedics
In this work new type of composite materials for application as coatings for intramedullary implants in the field of orthopaedics and traumatology is offered. Method is based on ability of fluoropolymers to act as biologically inert binding agent and ability of fine-dyspersated hydroxyapatite powders to act as biologically active filling agent providing osteoinduction and osteoconduction processes. Results of investigations of adhesion, elastic and morphometric characteristics of offered composite were presented; chemical composition was determined. Estimation of toxicological properties, locally irritant action and hemolytic activity of offered composites was done according to GOST R ISO 10993. In vivo tests were carried out; it was shown that offered composites didnβt cause any negative tissue reactions and stimulated osteogenesis processes in ectopic bone formation test. Key words: Carcass Compound Materials; fluoropolymers; orthopaedi
ΠΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΏΡΠ»Π° ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ
Modulating influence of electric impulses, visible light, some microelements is studied in parameters which are near to physio-logic ones on life activity of progenitor and mature cells of blood system in vitro and in situ. Content of granulocyte and fibroblastoid colony-forming units, concentration of colony-stimulating and colony-inhibiting activities, their concentration were determined , myelograms and morphologic signs of myelocariocytes apoptosis and necrosis were scored, saturation by chrom ions of peripheral blood erythrocytes was studied in mice bone marrow. The results showed that cell effects of physical-chemical influ-ences are small specific. Nevertheless, one may perform both positive and negative regulating influence on life activity of stromal precursors in dependence of energy density and stimulus concentration . The effect is due not only by direct but mediated reactions regulating direction and intensity of metabolic intracellular processes. It is suggested that one of target cells capable of change in-crease program of hemopoietic and stromal cells-progenitors of bone marrow in dependence of physical stimulus strength are lym-phocytes.ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ ΠΌΠΎΠ΄ΡΠ»ΠΈΡΡΡΡΠ΅Π΅ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠ»Π΅ΠΊΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΠΌΠΏΡΠ»ΡΡΠΎΠ², Π²ΠΈΠ΄ΠΈΠΌΠΎΠ³ΠΎ ΡΠ²Π΅ΡΠ°, Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠ°Ρ
, ΠΏΡΠΈΠ±Π»ΠΈΠΆΠ΅Π½Π½ΡΡ
ΠΊ ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌ, Π½Π° ΠΆΠΈΠ·Π½Π΅Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΡΠΎΠ΄ΠΎΠ½Π°ΡΠ°Π»ΡΠ½ΡΡ
ΠΈ Π·ΡΠ΅Π»ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠΈΡΡΠ΅ΠΌΡ ΠΊΡΠΎΠ²ΠΈ in vitro ΠΈ in situ. Π ΠΊΠΎΡΡΠ½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ ΠΌΡΡΠ΅ΠΉ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈΡΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π³ΡΠ°Π½ΡΠ»ΠΎΡΠΈΡΠ°ΡΠ½ΡΡ
(ΠΠΠ-Π) ΠΈ ΡΠΈΠ±ΡΠΎΠ±Π»Π°ΡΡΠΎΠΈΠ΄Π½ΡΡ
(ΠΠΠ-Π€) ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ΅ΠΎΠ±ΡΠ°Π·ΡΡΡΠΈΡ
Π΅Π΄ΠΈΠ½ΠΈΡ, ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ΅ΡΡΠΈΠΌΡΠ»ΠΈΡΡΡΡΠ΅ΠΉ ΠΈ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ΅ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ΅ΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠ΅ΠΉ, ΠΏΠΎΠ΄ΡΡΠΈΡΡΠ²Π°Π»ΠΈΡΡ ΠΌΠΈΠ΅Π»ΠΎΠ³ΡΠ°ΠΌΠΌΡ ΠΈ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΠΈ Π½Π΅ΠΊΡΠΎΠ·Π° ΠΌΠΈΠ΅Π»ΠΎΠΊΠ°ΡΠΈΠΎΡΠΈΡΠΎΠ², ΠΈΠ·ΡΡΠ°Π»ΠΎΡΡ Π½Π°ΡΡΡΠ΅Π½ΠΈΠ΅ ΠΈΠΎΠ½Π°ΠΌΠΈ Ρ
ΡΠΎΠΌΠ° ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ Π΄ΠΎΠ±ΡΠΎΠ²ΠΎΠ»ΡΡΠ°. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ ΡΡΡΠ΅ΠΊΡΡ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠΉ ΠΌΠ°Π»ΠΎΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½Ρ. Π’Π΅ΠΌ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ ΡΠ½Π΅ΡΠ³ΠΈΠΈ ΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΡΠ°Π·Π΄ΡΠ°ΠΆΠΈΡΠ΅Π»Ρ ΠΌΠΎΠΆΠ½ΠΎ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΡΡ ΠΊΠ°ΠΊ ΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΠΎΠ΅, ΡΠ°ΠΊ ΠΈ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠ΅ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠ΅ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° ΠΆΠΈΠ·Π½Π΅Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΊΡΠΎΠ²Π΅ΡΠ²ΠΎΡΠ½ΡΡ
ΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΡ
ΠΏΡΠ΅ΠΊΡΡΡΠΎΡΠΎΠ². ΠΡΠΈ ΡΡΠΎΠΌ ΡΡΡΠ΅ΠΊΡ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΏΡΡΠΌΡΠΌΠΈ, Π½ΠΎ ΠΈ ΠΎΠΏΠΎΡΡΠ΅Π΄ΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΡΠ΅ΡΠ΅Π· ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ΅ ΠΌΠΈΠΊΡΠΎΠΎΠΊΡΡΠΆΠ΅Π½ΠΈΠ΅ ΡΠ΅Π°ΠΊΡΠΈΡΠΌΠΈ, ΡΠ΅Π³ΡΠ»ΠΈΡΡΡΡΠΈΠΌΠΈ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΡΡΡ ΠΈ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΡ
Π²Π½ΡΡΡΠΈΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ². ΠΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΡΡΡ, ΡΡΠΎ ΠΎΠ΄Π½ΠΈΠΌΠΈ ΠΈΠ· ΠΊΠ»Π΅ΡΠΎΠΊ-ΠΌΠΈΡΠ΅Π½Π΅ΠΉ, ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΠΈΠ»Ρ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°Π·Π΄ΡΠ°ΠΆΠΈΡΠ΅Π»Ρ ΠΏΠ΅ΡΠ΅ΠΊΠ»ΡΡΠ°ΡΡ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ ΡΠΎΡΡΠ° ΠΊΡΠΎΠ²Π΅ΡΠ²ΠΎΡΠ½ΡΡ
ΠΈ ΡΡΡΠΎΠΌΠ°Π»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ-ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΈΠΊΠΎΠ² ΠΊΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, ΡΠ²Π»ΡΡΡΡΡ Π»ΠΈΠΌΡΠΎΡΠΈΡΡ
ΠΠ°ΠΊΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠΈ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²Ρ ΠΈ ΡΠΊΠ»Π΅ΡΡ ΠΏΠΎΡΠ»Π΅ ΠΈΠ½ΡΡΠ°ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠΈ ΡΠ°ΡΡΠ²ΠΎΡΠ° ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π Ρ ΠΊΡΠΎΠ»ΠΈΠΊΠΎΠ² ΡΠΎ ΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΡΡ Π³Π»Π°ΡΠΊΠΎΠΌΡ
Β Aim. In an in vivo experiment, to study the effect of local intraoperative application of 0.05% cyclosporin A solution on the conjunctival and scleral regeneration after surgery on the rabbit eyes with steroid-inducedΒ glaucoma.Materials and methods. At the first stage of the experiment, a model of steroid-induced glaucoma was reproduced for 29 male Californian rabbits by injecting 0.5 ml of a 0.4% solution of dexamethasone subconjunctivally in both eyes once a week for 3 months (12 subconjunctival injections for each rabbit). At the second stage of the experiment, after the development ofΒ steroid glaucoma, the rabbits were divided into the main group, consistingΒ of the subgroup Β«aΒ» (n = 8) and the subgroup Β«bΒ» (n = 8), and the comparison group (n = 8). All animals were performed a penetrating incision of the conjunctiva and a non-penetrating incision of the sclera of one of the eyes. A hemostatic sponge impregnated with 0.05% cyclosporin Π solution was applied to the intervention area in the main group, in the subgroup Β«aΒ» β for 3 minutes, in the subgroup Β«bΒ» β for 6 minutes. In the comparison group, the cytostatic was not used.Results. The use of 0.05% cyclosporin Π solution led to a decrease in theΒ infiltration of fibroblasts and inflammatory cells into the area of surgicalΒ injury. On the 4th day after the surgery, cell density in the intervention areaΒ in the subgroup Β«aΒ» with 3-minute application of cytostatic-antimetabolite solution was 2.7 times lower (p = 0.043) than in the comparison group, while exceeding the values in the subgroup Β«bΒ» by 3.2 times (p = 0.036). The number of fibroblasts in the subgroups Β«aΒ» and Β«bΒ» was 3.6 (p = 0.043) and 12.8 times (p = 0.031) less than in the comparison group, and a shift in the cellular composition of the infiltrate towards the fibroblastic population occurred only on the 14th day after the surgery.Conclusion. Intraoperative application of 0.05% cyclosporin Π solution significantly slows down the course of regeneration, reducing infiltrative inflammation in the intervention area, which prevents excessive scarring.Β Β Π¦Π΅Π»Ρ. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ in vivo ΠΈΠ·ΡΡΠΈΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΠΌΠ΅ΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΡΠ°ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠΈ 0,05%-Π³ΠΎ ΡΠ°ΡΡΠ²ΠΎΡΠ° (Ρ-ΡΠ°) ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π Π½Π° ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΡ ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²Ρ ΠΈ ΡΠΊΠ»Π΅ΡΡΒ ΠΏΠΎΡΠ»Π΅ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ Π½Π° Π³Π»Π°Π·Π°Ρ
ΠΊΡΠΎΠ»ΠΈΠΊΠΎΠ² ΡΠΎ ΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΠΎΠΉΒ Π³Π»Π°ΡΠΊΠΎΠΌΠΎΠΉ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ° I ΡΡΠ°ΠΏΠ΅ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° 29 ΡΠ°ΠΌΡΠ°ΠΌΒ ΠΊΡΠΎΠ»ΠΈΠΊΠΎΠ² ΠΊΠ°Π»ΠΈΡΠΎΡΠ½ΠΈΠΉΡΠΊΠΎΠΉ ΠΏΠΎΡΠΎΠ΄Ρ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π»ΠΈΒ ΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΡΡ Π³Π»Π°ΡΠΊΠΎΠΌΡ ΠΏΡΡΠ΅ΠΌ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΠΏΠΎΠ΄ ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²ΡΒ ΠΎΠ±ΠΎΠΈΡ
Π³Π»Π°Π· 0,5 ΠΌΠ» 0,4%-Π³ΠΎ Ρ-ΡΠ° Π΄Π΅ΠΊΡΠ°ΠΌΠ΅ΡΠ°Π·ΠΎΠ½Π° 1 ΡΠ°Π· Π² Π½Π΅Π΄ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 3 ΠΌΠ΅Ρ (12 ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ). ΠΠ° II ΡΡΠ°ΠΏΠ΅ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°, ΠΏΠΎΡΠ»Π΅ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΠΎΠΉ Π³Π»Π°ΡΠΊΠΎΠΌΡ, ΠΊΡΠΎΠ»ΠΈΠΊΠΎΠ² ΡΠ°Π·Π΄Π΅Π»ΠΈΠ»ΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π½ΡΡ Π³ΡΡΠΏΠΏΡ, ΡΠΎΡΡΠΎΡΡΡΡ ΠΈΠ· ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΡ Β«aΒ» (n = 8) ΠΈ ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΡ Β«bΒ» (n = 8), ΠΈ Π³ΡΡΠΏΠΏΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ (n = 8). ΠΡΠ΅ΠΌ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠΌ Π²ΡΠΏΠΎΠ»Π½ΡΠ»ΠΈ ΡΠΊΠ²ΠΎΠ·Π½ΠΎΠΉ ΡΠ°Π·ΡΠ΅Π· ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²Ρ ΠΈ Π½Π΅ΠΏΡΠΎΠ½ΠΈΠΊΠ°ΡΡΠΈΠΉ Π½Π°Π΄ΡΠ΅Π· ΡΠΊΠ»Π΅ΡΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈΠ· Π³Π»Π°Π·. ΠΠ° ΠΎΠ±Π»Π°ΡΡΡ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π° Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ Π½Π°ΠΊΠ»Π°Π΄ΡΠ²Π°Π»ΠΈ Π³Π΅ΠΌΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΡΡ Π³ΡΠ±ΠΊΡ, ΠΏΡΠΎΠΏΠΈΡΠ°Π½Π½ΡΡ 0,05%-ΠΌ Ρ-ΡΠΎΠΌ ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π, Π² ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΠ΅ Β«Π°Β» Π½Π° 3 ΠΌΠΈΠ½, Π² ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΠ΅ Β«bΒ» β Π½Π° 6 ΠΌΠΈΠ½. Π Π³ΡΡΠΏΠΏΠ΅ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΠΈΡΠΎΡΡΠ°ΡΠΈΠΊ Π½Π΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ 0,05%-Π³ΠΎ Ρ-ΡΠ° ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° ΠΒ ΠΏΡΠΈΠ²Π΅Π»ΠΎ ΠΊ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΡ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ Π·ΠΎΠ½Ρ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉΒ ΡΡΠ°Π²ΠΌΡ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΠΈ ΡΠΈΠ±ΡΠΎΠ±Π»Π°ΡΡΠ°ΠΌΠΈ. ΠΠ° 4- Π΅ ΡΡΡ ΠΏΠΎΡΠ»Π΅ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½Π°Ρ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡ Π² ΠΎΠ±Π»Π°ΡΡΠΈ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π° Π² ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΠ΅ Β«Π°Β» ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ Ρ ΡΡΠ΅Ρ
ΠΌΠΈΠ½ΡΡΠ½ΠΎΠΉ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠ΅ΠΉ Ρ-ΡΠ° ΡΠΈΡΠΎΡΡΠ°ΡΠΈΠΊΠ°-Π°Π½ΡΠΈΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ° Π±ΡΠ»Π° Π² 2,7 ΡΠ°Π·Π° ΠΌΠ΅Π½ΡΡΠ΅ (p = 0,043), ΡΠ΅ΠΌ Π²Β Π³ΡΡΠΏΠΏΠ΅ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ, ΠΏΡΠ΅Π²ΡΡΠ°Ρ ΠΏΡΠΈ ΡΡΠΎΠΌ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΡ Β«bΒ» Π² 3,2 ΡΠ°Π·Π° (p = 0,036). Π§ΠΈΡΠ»Π΅Π½Π½ΠΎΡΡΡΒ ΡΠΈΠ±ΡΠΎΠ±Π»Π°ΡΡΠΎΠ² Π² ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΠ°Ρ
Β«Π°Β» ΠΈ Β«bΒ» Π±ΡΠ»Π° Π² 3,6 (p = 0,043) ΠΈ 12,8 ΡΠ°Π·Π° (p = 0,031) Π½ΠΈΠΆΠ΅, ΡΠ΅ΠΌ Π² Π³ΡΡΠΏΠΏΠ΅ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ. ΠΡΠΈΒ ΡΡΠΎΠΌ ΡΠ΄Π²ΠΈΠ³ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠ° Π² ΡΡΠΎΡΠΎΠ½ΡΒ ΡΠΈΠ±ΡΠΎΠ±Π»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ ΠΏΡΠΎΠΈΠ·ΠΎΡΠ΅Π» ΡΠΎΠ»ΡΠΊΠΎ Π½Π° 14-Π΅Β ΡΡΡ ΠΏΠΎΡΠ»Π΅ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ.Β ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠ½ΡΡΠ°ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΡ 0,05%-Π³ΠΎ Ρ- ΡΠ° ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Π·Π°ΠΌΠ΅Π΄Π»ΡΠ΅Ρ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅Β Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎ-ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ, ΡΠΌΠ΅Π½ΡΡΠ°ΡΒ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠ²Π½ΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅ Π² Π·ΠΎΠ½Π΅ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π°, ΡΡΠΎΒ ΠΏΡΠ΅Π΄ΠΎΡΠ²ΡΠ°ΡΠ°Π΅Ρ ΠΈΠ·Π»ΠΈΡΠ½Π΅Π΅ ΡΡΠ±ΡΠ΅Π²Π°Π½ΠΈΠ΅.
ΠΠ°ΡΠΎΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²Ρ ΠΈ ΡΠΊΠ»Π΅ΡΡ Π½Π° ΡΠΎΠ½Π΅ ΠΈΠ½ΡΡΠ°ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠΈ ΡΠ°ΡΡΠ²ΠΎΡΠ° ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π
Purpose. In experiment in vivo to study the features of regeneration of the conjunctiva and sclera of rats after surgery with intraoperative application of a 0.05% Ciclosporin A.Materials and methods. Πxperimental animals (rats) (n = 48) were divided into the main group, including the subgroups a (n = 16) and b (n = 16) and the comparison group (n = 16). Performed a through cut of the conjunctiva and damage to the surface layers of the sclera one of the eyes of all animals. Further on the surgical trauma zone in the main group, the intraoperative application of the cytostatic was performed. In the subgroup a with a duration of 3 minutes, in the subgroup b β 6 minutes. In the comparison group a hemostatic sponge without a cytostatic was used intraoperatively.Results. In the comparison group postoperative period proceeds with a stereotyped dynamics of cell phase changes in damaged tissues. In the end the development of dense conjunctival-scleral fusion in the area of surgical trauma was noted. Intraoperative application of 0.05% Cyclosporine A leads to a slowing of regeneration, preventing formation of rough conjunctival-scleral scar.Conclusions. Intraoperative applications of 0.05% Cyclosporin A change the stereotyped dynamics of the inflammatory-reparative regeneration in the surgical intervention zone, inhibiting the migration of cells almost in 3 times and significantly (in 2 times) prolonging the duration of the macrophage phase. This causes a slowdown of reparative regeneration, prevents excessive scarring in the operating area.Β Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ: Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ in vivo ΠΈΠ·ΡΡΠΈΡΡ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²Ρ ΠΈ ΡΠΊΠ»Π΅ΡΡ ΠΊΡΡΡ ΠΏΠΎΡΠ»Π΅ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π° ΠΈ ΠΈΠ½ΡΡΠ°ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠΈ 0,05%-Π³ΠΎ ΡΠ°ΡΡΠ²ΠΎΡΠ° ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΠ΅ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅ (ΡΠ°ΠΌΡΡ ΠΊΡΡΡ, n = 48) Π±ΡΠ»ΠΈ ΡΠ°Π·Π΄Π΅Π»Π΅Π½Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π½ΡΡ Π³ΡΡΠΏΠΏΡ, Π²ΠΊΠ»ΡΡΠ°ΡΡΡΡ ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΡ Π° (n = 16) ΠΈ b (n = 16), ΠΈ Π³ΡΡΠΏΠΏΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ (n = 16). ΠΡΠ΅ΠΌ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠΌ Π²ΡΠΏΠΎΠ»Π½ΡΠ»ΠΈ ΡΠΊΠ²ΠΎΠ·Π½ΠΎΠΉ ΡΠ°Π·ΡΠ΅Π· ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²Ρ ΠΈ Π½Π΅ΠΏΡΠΎΠ½ΠΈΠΊΠ°ΡΡΠΈΠΉ Π½Π°Π΄ΡΠ΅Π· ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΡΡ
ΡΠ»ΠΎΠ΅Π² ΡΠΊΠ»Π΅ΡΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈΠ· Π³Π»Π°Π·. ΠΠ° ΠΎΠ±Π»Π°ΡΡΡ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠ°Π²ΠΌΡ Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ Π½Π°ΠΊΠ»Π°Π΄ΡΠ²Π°Π»Π°ΡΡ Π³Π΅ΠΌΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π³ΡΠ±ΠΊΠ°, ΠΏΡΠΎΠΏΠΈΡΠ°Π½Π½Π°Ρ 0,05%-ΠΌ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π: Π² ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΠ΅ Π° Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ 3 ΠΌΠΈΠ½, Π² ΠΏΠΎΠ΄Π³ΡΡΠΏΠΏΠ΅ b β 6 ΠΌΠΈΠ½. Π Π³ΡΡΠΏΠΏΠ΅ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΠΈΠ½ΡΡΠ°ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎ Π½Π°ΠΊΠ»Π°Π΄ΡΠ²Π°Π»Π°ΡΡ Π³Π΅ΠΌΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π³ΡΠ±ΠΊΠ° Π±Π΅Π· ΡΠΈΡΠΎΡΡΠ°ΡΠΈΠΊΠ°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ ΠΏΠ΅ΡΠΈΠΎΠ΄ ΠΏΡΠΎΡΠ΅ΠΊΠ°Π» ΡΠΎ ΡΡΠ΅ΡΠ΅ΠΎΡΠΈΠΏΠ½ΠΎΠΉ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΎΠΉ ΡΠΌΠ΅Π½Ρ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΡΠ°Π· Π² ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½Π½ΡΡ
ΡΠΊΠ°Π½ΡΡ
. Π ΠΈΡΡ
ΠΎΠ΄Π΅ ΠΎΡΠΌΠ΅ΡΠ΅Π½ΠΎ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΠΏΠ»ΠΎΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²Π°Π»ΡΠ½ΠΎ-ΡΠΊΠ»Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΡΠ΅Π½ΠΈΡ Π² Π·ΠΎΠ½Π΅ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠ°Π²ΠΌΡ. ΠΠ½ΡΡΠ°ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΡ 0,05%-ΠΌ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΊ Π·Π°ΠΌΠ΅Π΄Π»Π΅Π½ΠΈΡ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ, ΠΏΡΠ΅ΠΏΡΡΡΡΠ²ΠΎΠ²Π°Π»Π° ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π³ΡΡΠ±ΠΎΠ³ΠΎ ΠΊΠΎΠ½ΡΡΠ½ΠΊΡΠΈΠ²Π°Π»ΡΠ½ΠΎ-ΡΠΊΠ»Π΅ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ±ΡΠ°.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠ½ΡΡΠ°ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠ΅ Π°ΠΏΠΏΠ»ΠΈΠΊΠ°ΡΠΈΠΈ 0,05%-Π³ΠΎ ΡΠ°ΡΡΠ²ΠΎΡΠ° ΡΠΈΠΊΠ»ΠΎΡΠΏΠΎΡΠΈΠ½Π° Π ΠΌΠ΅Π½ΡΡΡ ΡΡΠ΅ΡΠ΅ΠΎΡΠΈΠΏΠ½ΡΡ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΡ ΡΠ΅ΡΠ΅Π½ΠΈΡ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎ-ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ Π² Π·ΠΎΠ½Π΅ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π°, ΠΏΠΎΠ΄Π°Π²Π»ΡΡ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π² ΡΡΠΈ ΡΠ°Π·Π° ΠΌΠΈΠ³ΡΠ°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΈ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ (Π² Π΄Π²Π° ΡΠ°Π·Π°) ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°Ρ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³Π°Π»ΡΠ½ΠΎΠΉ ΡΠ°Π·Ρ. ΠΡΠΎ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»ΠΈΠ²Π°Π΅Ρ Π·Π°ΠΌΠ΅Π΄Π»Π΅Π½ΠΈΠ΅ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ, ΠΏΡΠ΅ΠΏΡΡΡΡΠ²ΡΡΡΠ΅Π΅ ΠΈΠ·Π±ΡΡΠΎΡΠ½ΠΎΠΌΡ ΡΡΠ±ΡΠ΅Π²Π°Π½ΠΈΡ Π² ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π·ΠΎΠ½Π΅.
ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅Π°ΠΊΡΠΈΠΈ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠΎΠ² Π½Π° a-C:H:SiOx ΠΏΠΎΠΊΡΡΡΠΈΠ΅, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΠΎΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠ»Π°Π·ΠΌΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΡΠ°ΠΆΠ΄Π΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΈΠΌΠΏΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ Π±ΠΈΠΏΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΌΠ΅ΡΠ΅Π½ΠΈΡ
Aim. To study platelet adhesion to a-C:H:SiOx film on titanium in an in vitro experiment to evaluate itsΒ antithrombogenic potential.Materials and methods. Thin (less than 1 ΞΌm) a-C:H:SiOx films were deposited on VT-6 titanium plates with aΒ size of 10 Γ 10 mm2 and a thickness of 0.2 mm using a vacuum ion-plasma unit using pulsed bipolar bias. TheΒ surface roughness was evaluated according to GOST 2789-73 using an atomic force microscope. The test samplesΒ were cultured at 37 Β°C for 30 min in platelet-rich human blood plasma, prepared for scanning electron microscopy,Β after which the distribution density of blood plates adhering to the test coating was calculated.Results. With the same roughness index of the studied a-C:H:SiOx samples, the film decreased 116 times (inΒ comparison with untreated titanium) the platelet count per 1 mm2 of the surface.Conclusion. The deposition of a-C:H:SiOx thin film on the surface of VT-6 titanium alloy by PACVD methodΒ using pulsed bipolar bias significantly reduces the distribution density of platelets in comparison with an untreatedΒ metal surface. In vitro data suggest a significant antithrombogenic potential of this type of coating on the surfaceΒ of devices in contact with blood.Π¦Π΅Π»Ρ. ΠΠ·ΡΡΠΈΡΡ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ in vitro Π°Π΄Π³Π΅Π·ΠΈΡ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠΎΠ² ΠΊ a-C:H:SiOx ΠΏΠ»Π΅Π½ΠΊΠ΅ Π½Π° ΡΠΈΡΠ°Π½Π΅ Π΄Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ Π΅Π΅Β Π°ΡΡΠΎΠΌΠ±ΠΎΠ³Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π’ΠΎΠ½ΠΊΠΈΠ΅ (ΠΌΠ΅Π½Π΅Π΅ 1 ΠΌΠΊΠΌ) a-C:H:SiOx ΠΏΠ»Π΅Π½ΠΊΠΈ Π½Π°Π½ΠΎΡΠΈΠ»ΠΈ Π½Π° ΡΠΈΡΠ°Π½ΠΎΠ²ΡΠ΅ ΠΏΠ»Π°ΡΡΠΈΠ½Ρ ΠΌΠ°ΡΠΊΠΈΒ ΠΠ’-6 ΡΠ°Π·ΠΌΠ΅ΡΠΎΠΌ 10 Γ 10 ΠΌΠΌ2 ΠΈ ΡΠΎΠ»ΡΠΈΠ½ΠΎΠΉ 0,2 ΠΌΠΌ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π²Π°ΠΊΡΡΠΌΠ½ΠΎΠΉΒ ΠΈΠΎΠ½Π½ΠΎ-ΠΏΠ»Π°Π·ΠΌΠ΅Π½Π½ΠΎΠΉ ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΈΠΌΠΏΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ Π±ΠΈΠΏΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΌΠ΅ΡΠ΅Π½ΠΈΡ.Β Π¨Π΅ΡΠΎΡ
ΠΎΠ²Π°ΡΠΎΡΡΡ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ ΡΠΎΠ³Π»Π°ΡΠ½ΠΎΒ ΠΠΠ‘Π’ 2789-73 Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π°ΡΠΎΠΌΠ½ΠΎ-ΡΠΈΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠ°. ΠΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΏΡΠΈ 37 Β°CΒ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 30 ΠΌΠΈΠ½ Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Π½ΠΎΠΉ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ, ΠΏΠΎΠ΄Π³ΠΎΡΠ°Π²Π»ΠΈΠ²Π°Π»ΠΈ Π΄Π»Ρ ΡΠΊΠ°Π½ΠΈΡΡΡΡΠ΅ΠΉΒ ΡΠ»Π΅ΠΊΡΡΠΎΠ½Π½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ, ΠΏΠΎΡΠ»Π΅ ΡΠ΅Π³ΠΎ ΠΏΠΎΠ΄ΡΡΠΈΡΡΠ²Π°Π»ΠΈ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡΒ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΊΡΠΎΠ²ΡΠ½ΡΡ
ΠΏΠ»Π°ΡΡΠΈΠ½ΠΎΠΊ, Π°Π΄Π³Π΅Π·ΠΈΡΡΡΡΠΈΡ
ΠΊ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠΌΡ ΠΏΠΎΠΊΡΡΡΠΈΡ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠΈ ΠΎΠ΄ΠΈΠ½Π°ΠΊΠΎΠ²ΠΎΠΌ ΠΈΠ½Π΄Π΅ΠΊΡΠ΅ ΡΠ΅ΡΠΎΡ
ΠΎΠ²Π°ΡΠΎΡΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² a-C:H:SiOx ΠΏΠ»Π΅Π½ΠΊΠ° Π² 116 ΡΠ°Π·Β ΡΠ½ΠΈΠΆΠ°Π»Π° (Π² ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ Ρ Π½Π΅ΠΎΠ±ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠΌ ΡΠΈΡΠ°Π½ΠΎΠΌ) ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎΒ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠΎΠ² Π½Π° 1 ΠΌΠΌ2 ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π€ΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΡΠΈΡΠ°Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΏΠ»Π°Π²Π° ΠΠ’-6 ΡΠΎΠ½ΠΊΠΎΠΉ ΠΏΠ»Π΅Π½ΠΊΠΈ ΡΠΎΡΡΠ°Π²Π° a-C:H:SiOxΒ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠ»Π°Π·ΠΌΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΡΠ°ΠΆΠ΄Π΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌΒ ΠΈΠΌΠΏΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ Π±ΠΈΠΏΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΌΠ΅ΡΠ΅Π½ΠΈΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ½ΠΈΠΆΠ°Π΅Ρ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡΒ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠΎΠ² Π² ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ Ρ Π½Π΅ΠΎΠ±ΡΠ°Π±ΠΎΡΠ°Π½Π½ΠΎΠΉ ΠΌΠ΅ΡΠ°Π»Π»ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΡΡ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ in vitro Π΄Π°Π½Π½ΡΠ΅ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°ΡΡ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΉ Π°ΡΡΠΎΠΌΠ±ΠΎΠ³Π΅Π½Π½ΡΠΉ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π²ΠΈΠ΄Π°Β ΠΏΠΎΠΊΡΡΡΠΈΠΉ Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΡΡΡΡΠΎΠΉΡΡΠ², ΠΊΠΎΠ½ΡΠ°ΠΊΡΠΈΡΡΡΡΠΈΡ
Ρ ΠΊΡΠΎΠ²ΡΡ
STIMULATING EFFECT OF HIGH DOSE HEPARIN ON MIGRATION ACTIVITY AND MSC STEMNESS PRESERVATION IN THE PRESENCE OF BONE-SUBSTITUTING MATERIALS
Synthetic materials used in regenerative medicine, upon implantation, induce the development of an inflammatory reaction necessary for the effective regeneration of damaged bone tissue. Implant contact with tissues is accompanied by the deposition of blood proteins and interstitial fluid on its surface, contributing to the activation of the complement system, components of innate immunity, initiating coagulation hemostasis, leading to the formation of a fibrin clot. An extracellular matrix based on fibrin, collagen and elastin forms on the implantβs surface, which provides the basis for the formation of tissue structure through the adhesion of stem cells to the forming bone callus before the formation of bone regenerate. To prevent the development of postoperative pathological conditions caused by hypercoagulable syndrome, therapeutic strategies are used to use anticoagulants (heparin, warfarin). However, their use limits the normal formation of a fibrin clot in vivo. This can slow down the migration of mesenchymal stem cells (MSC) and disrupt the formation of callus, inhibiting the processes of osseointegration of the implant and bone healing. The studyβs goal was to study the effect of heparin in a gradient of low and high concentrations on the migration activity and stem capacity of human MSCs under in vitro cultivation conditions. According to the results of flow cytometry, it was revealed that high concentrations of heparin (130, 260 IU/ml) in a 2D cultivation model contribute to an increase in the number of cells expressing surface markers CD73 and CD90, which indicates that MSCs retain high clonogenic potential. A 3D model of in vitro cultivation with the addition of heparin and osteosubstituting implants bearing a CF coating with a roughness index of Ra = 2.6-4.9 ΞΌm contributed to preserving the βstemnessβ character of MSCs through the expression of surface markers CD73 and CD90. According to the results obtained using the xCELLigence system, heparin at a later time (from 20-40 hours) increases the invasion of MSCs through micropores that simulate the state of the blood vessel walls. However, in the presence of HAP nanoparticles that mimic the remodeling processes of the mineral bone matrix and/or resorption of bone cement, the effect of heparin was less pronounced. The results can be used in the field of regenerative medicine associated with the introduction of MSCs. The data can serve as a prerequisite for developing new therapeutic strategies for surgical patients with a high risk of postoperative thrombosis after osteosynthesis
Mesenchymal stem cells: a brief review of classis concepts and new factors of osteogenic differentiation
Molecular genetic mechanisms, signaling pathways, cultural conditions, factors, and markers of osteogenic differentiation of mesenchymal stem cells (MSC) are actively studied despite numerous works in this area of cellular technologies. This is largely due to the accumulating contradictions in seemingly classical knowledge, as well as permanent updating of the results in the field. In this regard, we focused on the main classical concepts and some new factors and mechanisms that have a noticeable regulatory effect on the differentiation potential of postnatal MSCs. The present review considers the significance of MSC sources for their differentiation capacity, as well as the role of the cellular microenvironment. The issues of classification, terminology, and functional activity of MSCs from various sources are discussed. The paracrine potential of MSCs in tissue regeneration has been considered; sufficient importance of inflammation in osteogenesis is noted, in particular, the presence of inflammatory cytokines and chemokines in the lesion focus, produced not only by microenvironmental cells but also by blood cells, including mononuclear leukocytes, migrating to the affected site. An important role in this review is given to biomechanical signals and to influence of conformational changes in cell cytoskeleton (cell shape) upon MSC differentiation, since the morphological features of cells and the structure of cytoskeleton are modulated by interactions of the cell surface with environmental factors, including hydrostatic pressure, fluid flow, compression/stretching loads. The data are presented concerning elasticity of extracellular matrix being a determining factor of cell differentiation. We conclude that one should switch from point studies of individual gene effects to multiple measurements of the gene-regulatory profile and biomolecules responsible for multiple, still poorly studied osteogenic factors of endogenous and exogenous origin. Among cornerstones in future (epi)genetic studies will be to decide if osteomodulatory effects are realized through specific signaling pathways and/or via cross-signaling with known genes controlling osteogenic differentiation of MSCs
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