17 research outputs found
Autolysis and ageing of Penicillium chrysogenum under carbon starvation: respiration and glucose oxidase production
During the exponential growth phase of Penicillium chrysogenum NCAIM 00237 the effective conversion of glucose and O2 to gluconate and H2O2 by glucose oxidase (GOX) was the most likely source of intracellular ROS measured. In glucose-supplemented autolysing cultures, the increased of intracellular ROS concentration was attributed to respiration in the absence of any significant GOX activity. The induction of GOX and catalase by glucose and H2O2 was clearly age-dependent in P. chrysogenum. In ageing cryptic growth phase cultures, superoxide dismutase and cyanide-resistant respiration were the major elements of antioxidative defence but these activities were insufficient to prevent the progressive accumulation of ROS and the concomitant decrease in cell vitality
Simultaneous detection of three porcine viruses by multiplex PCR
Specific oligonucleotide primers were selected and combined in a multiplex arrangement, in order to detect simultaneously three economically important porcine viruses by polymerase chain reaction (PCR). The pathogen panel was comprised of viruses that cause reproductive failure in infected herds: Aujeszky’s disease virus (ADV), porcine parvovirus (PPV) and porcine respiratory and reproductive syndrome virus (PRRSV). In order to reduce the time required for the detection of the pathogens, the assay was optimised to a RapidCycler PCR instrument. The multiplex PCR assay was shown to be specific, sensitive and rapid, because the results were read in less than 60 min after sample preparation. Due to its speed, efficiency and sensitivity, the described rapid multiplex PCR assay serves as a useful novel tool in the veterinary diagnostic laboratories for the quick and complex detection of these important porcine pathogens
Application of real-time rt-pcr utilising lux (light upon extension) fluorogenic primer for the rapid detection of avian influenza viruses
A real-time RT-PCR assay utilising light upon extension fluorogenic primer (LUX RT-PCR) was developed for the rapid and efficient detection of avian influenza viruses (AIV). The assay detected each of the AIV isolates tested (16/16) and gave negative results with heterologous pathogens (17/17). The detection limit of the assay proved to be 10-0.5 EID50/0.2 ml and 101.5 EID50/0.2 ml in allantoic fluid of virus-infected embryonated chicken eggs and in spiked chicken faeces samples, respectively. Based on its specificity, sensitivity and relative simplicity, the LUX RT-PCR assay provides a novel, rapid and cost-effective diagnostic tool for avian influenza surveillance and monitoring programs
Yeast-like cell formation and glutathione metabolism in autolysing cultures of Penicillium chrysogenum
The politics of organizing indigenous sport – cross-border and cross-sectoral complexity
Research Question: The authors studied the complexity surrounding the organization of sport in nations without nationstate status, exploring three research questions: (1) How do representatives of Sámi sport organizations in Finland and Norway perceive and act upon the proposal to reorganize Sámi sport? (2) How do representatives of the Sámi parliaments in Finland and Norway perceive and act upon the proposal to reorganize Sámi sport? (3) How do representatives of the state bureaucracy in Finland perceive and act upon the proposal to reorganize Sámi sport? Research Methods: We interviewed representatives of the Sámi parliaments and Sámi sport in Norway and Finland, an external consultant in Norway and a state official of Finland constituting a total of eight key actor interviews. Results and Findings: The analysis revealed the dominant role of the Norwegian side of Sámi sport, largely based on the institutional power of the Sámi parliament in Norway. In that respect, voluntary sport organizations in both Finland and Norway were reluctant to consider or were even negative towards an ongoing reorganization of Sámi sport since it was initiated by the Norwegian authorities and by the Norwegian Sámi parliament. Implications: Decision-makers in government agencies involved in cross-border and cross-sectoral issues should consider the opinions of all parties involved. Moreover, concerning indigenous groups striving for self-determination in post-colonial contexts, it is important to create a unified voice in matters important to the group as a collective