8 research outputs found

    Вплив умов одержання і збудження на спектральні і кінетичні характеристики катодолюмінесценції тонких плівок Y2O3:Eu

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    The spectra and kinetics of the rise and decay of the cathodoluminescence (CL) of thin films of Y2O3:Eu obtained by RF-magnetron sputtering were investigated. Based on the shape of the CL spectra at different excitation energies, it showed the possibility of creating irregular solutions of yttrium and europium oxides and the structural features of the surface and bulk layers. The time constant for the decay CL for 612 nm emission was determined and the value of which is within the range (1.8 - 4.1) ms. This value is a complex function of the type of film deposition atmosphere, the activator concentration, and the duration of the exciting pulses was shown. The features of the risen CL and the proposal based on the delayed rise CL analyze structural perfection of Y2O3:Eu  thin films.Досліджено спектри та кінетику розгоряння і загасання катодолюмінесценції (КЛ) тонких плівок Y2O3:Eu отриманих методом ВЧ-магнетронного напилення. На основі форми спектрів КЛ при різних енергіях збудження показано можливість утворення нерегулярних розчинів оксиду ітрію і європію та особливості структури поверхневих і об’ємних шарів. Визначено постійну часу загасання КЛ для свічення 612 нм, величина якої перебуває в межах (1,8 - 4,1) мс. Показано, що дана величина є складною функцією від виду атмосфери напилення плівок, концентрації активатора і тривалості збуджуючих імпульсів. Досліджено особливості розгоряння КЛ і запропоновано на основі затримки розгоряння КЛ аналізувати структурну досконалість тонких плівок Y2O3:Eu

    Individual Immune Responses to Chronic Stress and their Neuro-Endocrine Accompaniment, 200 s.

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    The monograph presents the literature data and the results of its own study of interindividual differences in the immune responses of rats of both sexes to chronic restraint stress and their neuro-endocrine accompaniment. The neuroendocrine-immune interrelations and sexual dimorphism are analyzed in detail. For physiologists, endocrinologists, immunologists

    Quantitative evaluation of integrated neuroendocrine and immune responses to chronic stress in rats male

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    Polovynko Іlona S, Zajats Lyubomyr М, Zukow Walery, Yanchij Roman I, Popovych Іgor L. Quantitative evaluation of integrated neuroendocrine and immune responses to chronic stress in rats male. Journal of Education, Health and Sport. 2016;6(8):154-166. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.60023 http://ojs.ukw.edu.pl/index.php/johs/article/view/3745 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.07.2016. Revised 25.07.2016. Accepted: 28.07.2016. QUANTITATIVE EVALUATION OF INTEGRATED NEUROENDOCRINE AND IMMUNE RESPONSES TO CHRONIC STRESS IN RATS MALE Іlona S Polovynko1, Lyubomyr М Zajats1, Walery Zukow2, Roman I Yanchij3, Іgor L Popovych3 1Department of Pathophysiology, National Medical University, Ivano-Frankivs’k, Ukraine [email protected] [email protected] 2Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland [email protected] 3Department of Immunophysiology, OO Bohomolets’ Institute of Physiology NAS, Kyiv, Ukraine [email protected] Abstract Background. Previously we have been analysed neuro-endocrine-immune relationships by chronic restraint stress at male rats. The purpose of this study - to carry out integrated quantitative estimation of neuroendocrine and immune responses to chronic restraint stress at male rats. Material and research methods. The experiment is at 50 white male rats. Of these 10 animals not subjected to any influences and 40 within 7 days subjected to moderate stress by daily 30-minute immobilization. The day after the completion of stressing in rats of both groups determined parameters of heart rate variability, urinary excretion of 17-ketosteroids, plasma levels of corticosterone, testosterone, thyroxine and triiodothyronine as well as sodium, potassium, calcium and phosphates. The same portion of the blood immunological parameters were determined by tests I and II levels of WHO. The spleen and thymus did smears for counting spleno- and thymocytograms. In sections of the adrenal glands was measured the thickness of glomerular, fascicular, reticular and medullar zones. Results. The method of discriminant analysis found that distinctive endocrine signs of chronic stress is increasing the thickness of Fasciculary Zone whereas decreasing thickness of Glomerulary Zone of Adrenal Cortex as well as plasma (Ca/P)0,5 ratio as Parathyrine Activity. Other signs of chronic stress such as increasing plasma levels Corticosterone, Testosterone and Triiodethyronine, Sympathetic tone, Heart Rate and thickness of Reticular Zone of Adrenals as well as decreasing Vagal Tone and plasma (Na/K)0,5 ratio as Mineralocorticoid Activity currently not in the discriminant model. Canonical Neuroendocrine Roots for Intact and Stressed Males Rats averages +0,99±0,40 and -0,25±0,15 respectively (Squared Mahalanobis Distance=1,61; F=3,76; p=0,017). Among the parameters of Immunity characteristic of chronic stress appeared to increase Thymus Massa Index, level in Thymocytogram of Macrophages and Reticulocytes, in Splenocytogram of Macrophages and Eosinophils, while Monocytes in Leukocytogram of Blood as well as Entropy of Leukocytogram and Splenocytogram whereas decrease both Intensity and Activity of Phagocytose by Neutrophils, levels of Endotheliocytes in Thymocytogram, Neutrophils in Splenocytogram, NK-Lymphocytes, Stub Neutrophils and Basophils in Leukocytogram. Canonical Immune Roots for Intact and Stressed Males Rats averages -3,41±0,40 and +0,85±0,15 respectively (Squared Mahalanobis Distance=19,0; F=5,44; p<10-4). Canonical correlation between Neuroendocrine and Immune parameters is very strong: R=0,976; χ2(297)=432; p<10-6. Conclusion. The components of the autonomic nervous, endocrine and immune systems interact closely within the triune neuroendocrine-immune complex and changing them exposed to integrated evaluation. The author's approach can be used to quantify the integrated assessment of intensity of stress factors and to integral assessing the effectiveness of stresslimiting factors. Keywords: chronic stress, autonomic regulation, adaptive hormones, adrenals, thymocytogram, splenocytogram, leukocytogram and immunocytogram of blood, relationships, male rats

    Neuroendocrine-immune relatioships in rats females

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    Background. Previously, in line with the concept of the neuroendocrine-immune complex, we analyzed the relationships between the parameters of the autonomic nervous and endocrine systems, on the one hand, and the parameters of immunity, on the other hand, in male rats. The purpose of this study is to analyze such interactions in female rats. Material and methods . In 60 females of rats, parameters of HRV, blood levels of hormones and electrolytes as well as parameters of leukocytogram, immunocytogram, thymocytogram and splenocytogram were determined. The coefficients of canonical correlation R between neuroendocrine parameters, on the one hand, and parameters of immunity, on the other hand, were calculated. Results. The following values of R for neuroendocrine parameters were found. Sympathetic tone: 0,702; Vagal tone: 0,756; Moda HRV: 0,896; the thickness of the Fascicular zone of adrenal cortex: 0,727; Glomerular zone: 0,650; Reticular zone: 0,442; plasma level of Corticosterone: 0,601; Testosterone: 0,753; Triiodo-thyronine: 0,544; Thyroxine: 0,441; Mineralocorticoid activity: 0,474; Calcitonine activity: 0,580; Parathyrine activity: 0,551. Conclusion . The results obtained by us complement and specify the concept of a triune neuroendocrine-immune complex

    Quantitative evaluation of integrated neuroendocrine and immune responses to chronic stress in rats male

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    Polovynko Іlona S, Zajats Lyubomyr М, Zukow Walery, Yanchij Roman I, Popovych Іgor L. Quantitative evaluation of integrated neuroendocrine and immune responses to chronic stress in rats male. Journal of Education, Health and Sport. 2016;6(8):154-166. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.60023 http://ojs.ukw.edu.pl/index.php/johs/article/view/3745       The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.07.2016. Revised 25.07.2016. Accepted: 28.07.2016.     QUANTITATIVE EVALUATION OF INTEGRATED NEUROENDOCRINE AND IMMUNE RESPONSES TO CHRONIC STRESS IN RATS MALE   Іlona S Polovynko1, Lyubomyr М Zajats1, Walery Zukow2, Roman I Yanchij3, Іgor L Popovych3   1Department of Pathophysiology, National Medical University, Ivano-Frankivs’k, Ukraine   [email protected]   [email protected] 2Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland   [email protected] 3Department of Immunophysiology, OO Bohomolets’ Institute of Physiology NAS, Kyiv, Ukraine   [email protected]     Abstract Background. Previously we have been analysed neuro-endocrine-immune relationships by chronic restraint stress at male rats. The purpose of this study - to carry out integrated quantitative estimation of neuroendocrine and immune responses to chronic restraint stress at male rats. Material and research methods. The experiment is at 50 white male rats. Of these 10 animals not subjected to any influences and 40 within 7 days subjected to moderate stress by daily 30-minute immobilization. The day after the completion of stressing in rats of both groups determined parameters of heart rate variability, urinary excretion of 17-ketosteroids, plasma levels of corticosterone, testosterone, thyroxine and triiodothyronine as well as sodium, potassium, calcium and phosphates. The same portion of the blood immunological parameters were determined by tests I and II levels of WHO. The spleen and thymus did smears for counting spleno- and thymocytograms. In sections of the adrenal glands was measured the thickness of glomerular, fascicular, reticular and medullar zones. Results. The method of discriminant analysis found that distinctive endocrine signs of chronic stress is increasing the thickness of Fasciculary Zone whereas decreasing thickness of Glomerulary Zone of Adrenal Cortex as well as plasma (Ca/P)0,5 ratio as Parathyrine Activity. Other signs of chronic stress such as increasing plasma levels Corticosterone, Testosterone and Triiodethyronine, Sympathetic tone, Heart Rate and thickness of Reticular Zone of Adrenals as well as decreasing Vagal Tone and plasma (Na/K)0,5 ratio as Mineralocorticoid Activity currently not in the discriminant model. Canonical Neuroendocrine Roots for Intact and Stressed Males Rats averages +0,99±0,40 and -0,25±0,15 respectively (Squared Mahalanobis Distance=1,61; F=3,76; p=0,017). Among the parameters of Immunity characteristic of chronic stress appeared to increase Thymus Massa Index, level in Thymocytogram of Macrophages and Reticulocytes, in Splenocytogram of Macrophages and Eosinophils, while Monocytes in Leukocytogram of Blood as well as Entropy of Leukocytogram and Splenocytogram whereas decrease both Intensity and Activity of Phagocytose by Neutrophils, levels of Endotheliocytes in Thymocytogram, Neutrophils in Splenocytogram, NK-Lymphocytes, Stub Neutrophils and Basophils in Leukocytogram. Canonical Immune Roots for Intact and Stressed Males Rats averages -3,41±0,40 and +0,85±0,15 respectively (Squared Mahalanobis Distance=19,0; F=5,44; p<10-4). Canonical correlation between Neuroendocrine and Immune parameters is very strong: R=0,976; χ2(297)=432; p<10-6. Conclusion. The components of the autonomic nervous, endocrine and immune systems interact closely within the triune neuroendocrine-immune complex and changing them exposed to integrated evaluation. The author's approach can be used to quantify the integrated assessment of intensity of stress factors and to integral assessing the effectiveness of stresslimiting factors.   Keywords: chronic stress, autonomic regulation, adaptive hormones, adrenals, thymocytogram, splenocytogram, leukocytogram and immunocytogram of blood, relationships, male rats
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