DAUN SIRIH MERAH Manfaat Untuk Kesehatan

Indonesia memiliki beragam potensi alam yang memiliki kemampuan untuk pengobatan alternative bagi penyakit yang ada. Terapi pengobatan menggunakan tanaman yang ada di alam sudah banyak diterapkan saat ini karena tidak memiliki efek samping dan dapat digunakan dalam jangka panjang. Salah satu bahan alam yang mempunyai berbagai keunggulan bagi kesehatan adalah sirih merah. Sirih merah sudah dikonsumsi sejak dahulu, menjadi budaya, dan terbukti mempunyai nilai positif bagi kesehatan karena berbagai kandungan di dalamnya. Buku ini mengupas segala sisi tentang sirih merah dan pengaruhnya dalam menunjang kesehatan manusia. Kandungan senyawa bioaktif yang beragam di dalam sirih merah mampu berperan menjaga kesehatan dan dapat memperbaiki fungsi tubuh. Buku ini menjelaskan peran sirih   merah   dengan   sangat   detail.   Setiap   bab   juga dilengkapi dengan glosarium dan daftar singkatan untuk memudahkan dalam memahami isi bab tersebut. Pembahasan tentang sirih merah melalui buku ini dapat membantu pembaca untuk lebih memahami sirih merah dan memaksimalkan potensi sirih merah sehingga kesejahteraan manusia dapat ditingkatkan. Ucapan terima kasih penulis haturkan kepada semua pihak yang mendukung   penerbitan   buku   ini.   Kepada   para   team peneliti    Aretha    Medika    Utama,    Biomolecular    and Biomedical Research Center, Bandung yang telah banyak membantu dalam menyusun naskah buku sampai penerbitan

Low Levels of Amyloid Precursor Protein (APP) Promote Neurogenesis and Decrease Gliogenesis in Human Neural Stem Cells

Amyloid precursor protein (APP) has been widely studied due to its association with Alzheimer's disease (AD). However, the physiological functions of APP are still largely unexplored. APP is a transmembrane glycoprotein whose expression in humans is abundant in the central nervous system. Specifically, several studies have revealed the high expression of APP during brain development. Previous studies in our laboratory revealed that a transient increase in APP expression induces early cell cycle exit of human neural stem cells (hNSCs) and directs their differentiation towards glial cells (gliogenesis) while decreasing their differentiation towards neurons (neurogenesis). In the present study, we have evaluated the intrinsic cellular effects of APP down-expression (using siRNA) on cell death, cell proliferation, and cell fate specification of hNSCs. Our data indicate that APP silencing causes cellular effects opposite to those obtained in previous APP overexpression assays, inducing cell proliferation in hNS1 cells (a model line of hNSCs) and favoring neurogenesis instead of gliogenesis in these cells. In addition, we have analyzed the gene and protein expression levels of β-Catenin as a possible molecule involved in these cellular effects. These data could help to understand the biological role of APP, which is necessary to deepen the knowledge of AD.This research was supported by a grant from the Spanish Ministry of Science and Innovation (RTI2018-101663-B-100) and grant number PID2021-126715OB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. R.C. was supported by an FPU predoctoral contract from Universidad de Alcalá (FPU-UAH).S

Manipulation of the phenotypic appearance of individuals in groups of laying hens: Effects on stress and immune-related variables

This study evaluated whether phenotypic appearance (PA) alteration during two developmental phases in laying hens, reared in two different group sizes, affects stress and immune responses. After hatching, 750 chicks were randomly assigned to 30 pens at a group size of either 10 or 40 birds. Then, the appearance of 0, 30, 50, 70 or 100% of the chicks in each pen was altered by blackdyeing their head feathers (marked); remaining chicks were unmarked. At 32 weeks, basal and postacute stress plasma corticosterone concentration, leukocyte counts, phytohemagglutinin-p lymphoproliferative and primary antibody responses were measured in six birds/pen. Analysis of variances (ANOVAs) showed no differences among treatment combinations. In a second phase, birds within initially homogeneous pens were sequentially either marked or had dye bleached to alter PA of 70% of hens in each flock (= group in a pen). Hens within initially heterogeneous pens remained unaltered as controls. The above variables were remeasured. Hens in phenotypically manipulated pens showed modified leukocyte counts compared to hens in control pens, indicating a chronic stress reaction in all penmates (whether individual PA was altered or not). Social isolation increased plasma corticosterone concentration. However, within groups of n = 40, phenotypically unaltered hens had lower responses than their altered penmate counterparts, suggesting that remaining in a stable PA group AIDS better coping with challenges. Although all hens in manipulated pens showed modified leukocyte counts, their antibody and lymphoproliferative responses did not differ from controls suggesting that all groupmates were able to immunologically cope with the challenges presented, within the timeframe evaluated.Fil: Nazar, Franco Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Marin, Raul Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Liste, Mariano Gonzalo. Centro de Investigación Neiker - Tecnalia; EspañaFil: Campderrich, I.. Centro de Investigación Neiker - Tecnalia; EspañaFil: Estevez, I.. Centro de Investigación Neiker - Tecnalia; Españ

Impact assessment for the improved four boundary conditions (at bed, free-surface, land-boundary and offshore-boundary) on coastal hydrodynamics and particulate transport

The FIELD_AC project aims at providing an improved operational service for coastal areas and at generating added value for shelf and regional scale predictions. Coastal-zone oceanographic predictions seldom appraise the land discharge as a boundary condition. River fluxes are sometimes considered, but neglecting their 3D character, while the "distributed" continental run-off is not taken into consideration. Moreover, many coastal scale processes, particularly those relevant in geographically restricted domains (coast with harbors or river mouth areas), are not well parametrized in present simulations.Work package 3 dedicated to Boundary Fluxes aims to establish and use the best possible boundary conditions for coastal water quality modelling. On this scale, all boundaries become important. For the land boundary side the needed products are distributed and point wise run-off both quantitatively and qualitatively. For the offshore boundary condition, 3D current, water quality field, and wave spectra will be used. For the atmospheric boundary, products from local scale meteorological models (wind, atmospheric pressure and rainfall) are needed. For the seabed, boundary information on sediment composition, bedforms and bathymetry and bio-geo-chemical parameters is essential.This report addresses the impact assessment for improvements in the four boundary conditions (boundary fluxes from land, free-surface boundary condition, seabed boundary condition and open boundary fluxes) on coastal hydrodynamics and particulate transport. The description of the improved four boundary conditions is followed by examples of concrete impact assessment of the theory into the Catalan coast, Liverpool Bay, German Bight and Gulf of Venice

Human cortical organoids expose a differential function of GSK3 on cortical neurogenesis

The regulation of the proliferation and polarity of neural progenitors is crucial for the development of the brain cortex. Animal studies have implicated glycogen synthase kinase 3 (GSK3) as a pivotal regulator of both proliferation and polarity, yet the functional relevance of its signaling for the unique features of human corticogenesis remains to be elucidated. We harnessed human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Single-cell transcriptome profiling revealed a direct impact on early neurogenesis and uncovered a selective role of GSK3 in the regulation of glutamatergic lineages and outer radial glia output. Our dissection of the GSK3-dependent transcriptional network in human corticogenesis underscores the robustness of the programs determining neuronal identity independent of tissue architecture

Just use it! Linguistic conversion and identities of resistance amongst Galician new speakers

In recent years there has been a focus in language policy research on understanding how national policies are interpreted and negotiated by social actors on the ground. This paper looks at the interplay between government and grassroots initiatives to create Galician-speaking spaces in predominantly Spanish-speaking urban settings. While official language policies in Galicia since the 1980s have increased the potential for language use through bilingual educational policies, these policies have failed to convert the large pool of potential speakers amongst a younger generation of Galicians into active language users. Drawing on ethnographic fieldwork and in-depth interviews with Galician neofalantes (new speakers) this paper looks at instances where such policies seem to have worked and where the linguistic capacity created through the education system has been converted into active language use. The article examines how such speakers rationalise their practice of linguistic conversion not as success stories of language policy but as reactions to and dissatisfaction with what is perceived as ‘top-down’ governmentality through a reflexive process in which existing power structures are brought into question. The article looks specifically as the ideologies underpinning their decisions to become active speakers and the role they play as language planners in contemporary Galicia

Clonal human fetal ventral mesencephalic dopaminergic neuron precursors for cell therapy research

A major challenge for further development of drug screening procedures, cell replacement therapies and developmental studies is the identification of expandable human stem cells able to generate the cell types needed. We have previously reported the generation of an immortalized polyclonal neural stem cell (NSC) line derived from the human fetal ventral mesencephalon (hVM1). This line has been biochemically, genetically, immunocytochemically and electrophysiologically characterized to document its usefulness as a model system for the generation of A9 dopaminergic neurons (DAn). Long-term in vivo transplantation studies in parkinsonian rats showed that the grafts do not mature evenly. We reasoned that diverse clones in the hVM1 line might have different abilities to differentiate. In the present study, we have analyzed 9 hVM1 clones selected on the basis of their TH generation potential and, based on the number of v-myc copies, v-myc down-regulation after in vitro differentiation, in vivo cell cycle exit, TH+ neuron generation and expression of a neuronal mature marker (hNSE), we selected two clones for further in vivo PD cell replacement studies. The conclusion is that homogeneity and clonality of characterized NSCs allow transplantation of cells with controlled properties, which should help in the design of long-term in vivo experimentsThis work was supported by grants from the Spanish Ministry of Economy and Competitiveness (formerly Science and Innovation; PLE2009-0101, SAF2010-17167), Comunidad Autónoma Madrid (S2011-BMD-2336), Instituto Salud Carlos III (RETICS TerCel, RD06/0010/0009) and European Union (Excell, NMP4-SL-2008-214706). This work was also supported by an institutional grant from Foundation Ramón Areces to the Center of Molecular Biology Severo Ocho

Limited duration of vaccine poliovirus and other enterovirus excretion among human immunodeficiency virus infected children in Kenya

<p>Abstract</p> <p>Background</p> <p>Immunodeficient persons with persistent vaccine-related poliovirus infection may serve as a potential reservoir for reintroduction of polioviruses after wild poliovirus eradication, posing a risk of their further circulation in inadequately immunized populations.</p> <p>Methods</p> <p>To estimate the potential for vaccine-related poliovirus persistence among HIV-infected persons, we studied poliovirus excretion following vaccination among children at an orphanage in Kenya. For 12 months after national immunization days, we collected serial stool specimens from orphanage residents aged <5 years at enrollment and recorded their HIV status and demographic, clinical, immunological, and immunization data. To detect and characterize isolated polioviruses and non-polio enteroviruses (NPEV), we used viral culture, typing and intratypic differentiation of isolates by PCR, ELISA, and nucleic acid sequencing. Long-term persistence was defined as shedding for ≥ 6 months.</p> <p>Results</p> <p>Twenty-four children (15 HIV-infected, 9 HIV-uninfected) were enrolled, and 255 specimens (170 from HIV-infected, 85 from HIV-uninfected) were collected. All HIV-infected children had mildly or moderately symptomatic HIV-disease and moderate-to-severe immunosuppression. Fifteen participants shed vaccine-related polioviruses, and 22 shed NPEV at some point during the study period. Of 46 poliovirus-positive specimens, 31 were from HIV-infected, and 15 from HIV-uninfected children. No participant shed polioviruses for ≥ 6 months. Genomic sequencing of poliovirus isolates did not reveal any genetic evidence of long-term shedding. There was no long-term shedding of NPEV.</p> <p>Conclusion</p> <p>The results indicate that mildly to moderately symptomatic HIV-infected children retain the ability to clear enteroviruses, including vaccine-related poliovirus. Larger studies are needed to confirm and generalize these findings.</p