Spectroscopic characterization of reaction centers of the (M)Y210W mutant of the photosynthetic bacterium Rhodobacter sphaeroides

The tyrosine-(M)210 of the reaction center of Rhodobacter sphaeroides 2.4.1 has been changed to a tryptophan using site-directed mutagenesis. The reaction center of this mutant has been characterized by low-temperature absorption and fluorescence spectroscopy, time-resolved sub-picosecond spectroscopy, and magnetic resonance spectroscopy. The charge separation process showed bi-exponential kinetics at room temperature, with a main time constant of 36 ps and an additional fast time constant of 5.1 ps. Temperature dependent fluorescence measurements predict that the lifetime of P* becomes 4–5 times slower at cryogenic temperatures. From EPR and absorbance-detected magnetic resonance (ADMR, LD-ADMR) we conclude that the dimeric structure of P is not significantly changed upon mutation. In contrast, the interaction of the accessory bacteriochlorophyll BA with its environment appears to be altered, possibly because of a change in its position

Integrating Systems around the User: Combining Isabelle, Maple, and QEPCAD in the Prover's Palette

AbstractWe describe the Proverʼs Palette, a general, modular architecture for combining tools for formal verification, with the key differentiator that the integration emphasises the role of the user. A concrete implementation combining the theorem prover Isabelle with the computer algebra systems Maple and QEPCAD-B is then presented. This illustrates that the design principles of the Proverʼs Palette simplify tool integrations while enhancing the power and usability of theorem provers

Brief comments on Jackiw-Teitelboim gravity coupled to Liouville theory

Jackiw-Teitelboim gravity with non-vanishing cosmological constant coupled to Liouville theory is considered as a non-critical string on $d$ dimensional flat spacetime. It is discussed how the presence of cosmological constant yields additional constraints on the parameter space of the theory, even when the conformal anomaly is independent of the cosmological constant. Such constraints agree with the necessary conditions for the tachyon field to be a primary --prelogarithmic-- operator of the worldsheet conformal field theory. Thus, the linearized tachyon field equation allows to impose the diagonal condition for the interaction term. We analyze the neutralization of the Liouville mode induced by the coupling to the Jackiw-Teitelboim Lagrangian. The free field prescription leads to obtain explicit expressions for three-point correlation functions for the case of vanishing cosmological constant in terms of a product of Shapiro-Virasoro integrals. This is a consequence of the mentioned neutralization effect.Comment: 14 pages, no figures. v2 References added. To be published in Classical and Quantum Gravity. v3 typos correcte

The role of rs2237781 within GRM8 in eating behavior

Introduction: The glutamate receptor, metabotropic 8 gene (GRM8) encodes a G-protein-coupled glutamate receptor and has been associated with smoking behavior and liability to alcoholism implying a role in addiction vulnerability. Data from animal studies suggest that GRM8 may be involved in the regulation of the neuropeptide Y and melanocortin pathways and might influence food intake and metabolism. This study aimed to investigate the effects of the genetic variant rs2237781 within GRM8 on human eating behavior. Methods: The initial analysis included 548 Sorbs from Germany who have been extensively phenotyped for metabolic traits and who completed the German version of the three-factor eating questionnaire. In addition, we analyzed two independent sample sets comprising 293 subjects from another German cohort and 430 Old Order Amish individuals. Genetic associations with restraint, disinhibition, and hunger were assessed in an additive linear regression model. Results: Among the Sorbs the major G allele of rs2237781 was significantly associated with increased restraint scores in eating behavior (P = 1.9 9 10?4; b =+1.936). The German cohort and the Old Order Amish population revealed a trend in the same direction for restraint (P = 0.242; b =+0.874; P = 0.908; b =+0.096; respectively). A meta-analysis resulted in a combined P = 3.1 9 10?3 (Z-score 2.948). Conclusion: Our data suggest that rs2237781 within GRM8 may influence human eating behavior factors probably via pathways involved in addictive behavior

AGATA, Technical Proposal for an Advanced Gamma Tracking Array for the European Gamma Spectroscopy Community

International audienceAn Advanced GAmma-ray Tracking Array, AGATA, is proposed for high-resolution γ-ray spectroscopy with exotic beams. AGATA will employ highly segmented Ge detectors as well as fully digital electronics and relies on newly developed pulse-shape analysis and tracking methods. The array is being designed in a way that it provides optimal properties for nuclear structure experiments in a wide range of beam velocities (from stopped to v/c ≈ 50%), almost independent of beam quality and background conditions. Selectivity and sensitivity of AGATA will be superior to any existing γ-array by several orders of magnitude. Hence, it will be for a long time a rich source for nuclear structure physics providing the means for new discoveries and opening challenging new perspectives. This document is the initial proposal sent to the European Commission to obtain the necessary funds for the project

Influence of polymer excluded volume on the phase behavior of colloid-polymer mixtures

We determine the depletion-induced phase-behavior of hard sphere colloids and interacting polymers by large-scale Monte Carlo simulations using very accurate coarse-graining techniques. A comparison with standard Asakura-Oosawa model theories and simulations shows that including excluded volume interactions between polymers leads to qualitative differences in the phase diagrams. These effects become increasingly important for larger relative polymer size. Our simulations results agree quantitatively with recent experiments.Comment: 5 pages, 4 figures submitted to Physical Review Letter

Structure of Colloid-Polymer Suspensions

We discuss structural correlations in mixtures of free polymer and colloidal particles based on a microscopic, 2-component liquid state integral equation theory. Whereas in the case of polymers much smaller than the spherical particles the relevant polymer degree of freedom is the center of mass, for polymers larger than the (nano-) particles conformational rearrangements need to be considered. They have the important consequence that the polymer depletion layer exhibits two widely different length scales, one of the order of the particle radius, the other of the order of the polymer radius or the polymer density screening length in dilute or semidilute concentrations, respectively. Their consequences on phase stability and structural correlations are discussed extensively.Comment: 37 pages, 17 figures; topical feature articl

Phase equilibria and glass transition in colloidal systems with short-ranged attractive interactions. Application to protein crystallization

We have studied a model of a complex fluid consisting of particles interacting through a hard core and a short range attractive potential of both Yukawa and square-well form. Using a hybrid method, including a self-consistent and quite accurate approximation for the liquid integral equation in the case of the Yukawa fluid, perturbation theory to evaluate the crystal free energies, and mode-coupling theory of the glass transition, we determine both the equilibrium phase diagram of the system and the lines of equilibrium between the supercooled fluid and the glass phases. For these potentials, we study the phase diagrams for different values of the potential range, the ratio of the range of the interaction to the diameter of the repulsive core being the main control parameter. Our arguments are relevant to a variety of systems, from dense colloidal systems with depletion forces, through particle gels, nano-particle aggregation, and globular protein crystallization.Comment: 20 pages, 10 figure

The discovery of a radio galaxy of at least 5 Mpc

We discover what is in projection the largest known structure of galactic origin: a giant radio galaxy with a projected proper length of $4.99 \pm 0.04\ \mathrm{Mpc}$. The source, named Alcyoneus, was first identified in low-resolution LOFAR Two-metre Sky Survey images from which angularly compact sources had been removed. Being an extreme example in its class, Alcyoneus could shed light on the main mechanisms that drive radio galaxy growth. We find that - beyond geometry - Alcyoneus and its host galaxy appear suspiciously ordinary: the total low-frequency luminosity density, stellar mass and supermassive black hole mass are all lower than, though similar to, those of the medial giant radio galaxy (percentiles$45 \pm 3\%$,$25 \pm 9 \%$and$23 \pm 11 \%$, respectively). The source resides in a filament of the Cosmic Web, with which it might have significant thermodynamic interaction. At$5 \cdot 10^{-16}\ \mathrm{Pa}$, the pressures in the lobes are the lowest hitherto found, and Alcyoneus therefore represents one of the most promising radio galaxies yet to probe the warm-hot intergalactic medium.Comment: 18 pages, 14 figures, 3 tables, accepted for publication in Astronomy & Astrophysic