273 research outputs found
Chemical composition, antibacterial activity of essential oil and anatomical study of Chrysanthemum morifolium
The aim of this study is to identify the chemical composition and to evaluate the antimicrobial activity of Chrysanthemum morifolium. The analysis and identification of essential oil which obtained by hydrodistilation method were realized by gaz chromatography and mass spectroscopy. The antibacterial activity was tested by using the agar diffusion test and the Gram positive and negative pathogenic bacteria: Staphylococcus aureus ATCC 25923. Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Citrobacter freundii ATCC 8090, Kleibseilla pneumoniae ATCC 700603 and Shigella sonnei were used to evaluate this activity. This analysis led to the identification of 26 compounds representing 88.40 % of the total essential oil mass. The major compound was Verbenone (17.33 %). Other components present in appreciable contents were: Chrysanthenone (9.71%). 4-epi-cubedol (07.25%) and Ī“-Cadinol (05.29 %). Essential oil of Chrysanthemum morifolium exhibited an antibacterial effect against pathogenic bacteria, like those observed against Staphylococcus aureus ATCC 25923 (35Ā±1.2mm) and Citrobacter freundii ATCC 8090 (21Ā±0.87mm), however Pseudomonas aeruginosa ATCC 27853 and Kleibseilla pneumoniae ATCC 700603 were resistant. The anatomical study showed the presence of several types of trichomes including the glands secreted for essential oils and protector trichomes.
Keywords: essential oil, antibacterial activity, Chrysanthemum morifolium, anatomical study, chemical compositio
Automatic categorization and summarization of documentaries
In this paper, we propose automatic categorization and summarization of documentaries using subtitles of videos. We propose two methods for video categorization. The first makes unsupervised categorization by applying natural language processing techniques on video subtitles and uses the WordNet lexical database and WordNet domains. The second has the same extraction steps but uses a learning module to categorize. Experiments with documentary videos give promising results in discovering the correct categories of videos. We also propose a video summarization method using the subtitles of videos and text summarization techniques. Significant sentences in the subtitles of a video are identified using these techniques and a video summary is then composed by finding the video parts corresponding to these summary sentences. Ā© 2010 The Author(s)
Summarization of documentaries
Video summarization algorithms present condensed versions of a full length video by identifying the most significant parts of the video. In this paper, we propose an automatic video summarization method using the subtitles of videos and text summarization techniques. We identify significant sentences in the subtitles of a video by using text summarization techniques and then we compose a video summary by finding the video parts corresponding to these summary sentences. Ā© 2011 Springer Science+Business Media B.V
What should we call the Levant mole? Unravelling the systematics and demography of Talpa levantis Thomas, 1906 sensu lato (Mammalia: Talpidae)
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Microstructural, Biomechanical, and In Vitro Studies of Ti-Nb-Zr Alloys Fabricated by Powder Metallurgy
Data Availability Statement:
The data underpinning this publication can be accessed from Brunel University Londonās data repository, Brunelfigshare here under a CCBY licence: https://doi.org/10.17633/rd.brunel.5446813.v1. Non-digital data supporting this study are stored by the corresponding author at Brunel University. Details of how to request access to these data are provided here: https://doi.org/10.17633/rd.brunel.5446813.v1.Supplementary Materials: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ma16124240/s1, Figure S1: PlasmidāDNA interaction assay for Ti-Nb-Zr based alloys. The migration pattern of plasmid DNA incubated with Ti-Nb-Zr-based alloys and TiRG4 reference materials is shown in the figure. The bands are labeled as NC: Nicked circular and SC: Supercoiled; ddH2O served as a negative control.Copyright Ā© 2023 by the authors. This study investigated the microstructures, mechanical performances, corrosion resistances, and in vitro studies of porous Ti-xNb-10Zr (x: 10 and 20; at. %) alloys. The alloys were fabricated by powder metallurgy with two categories of porosities, i.e., 21ā25% and 50ā56%, respectively. The space holder technique was employed to generate the high porosities. Microstructural analysis was performed by using various methods including scanning electron microscopy, energy dispersive spectroscopy, electron backscatter diffraction, and x-ray diffraction. Corrosion resistance was assessed via electrochemical polarisation tests, while mechanical behavior was determined by uniaxial compressive tests. In vitro studies, such as cell viability and proliferation, adhesion potential, and genotoxicity, were examined by performing an MTT assay, fibronectin adsorption, and plasmid-DNA interaction assay. Experimental results showed that the alloys had a dual-phase microstructure composed of finely dispersed acicular hcp Ī±-Ti needles in the bcc Ī²-Ti matrix. The ultimate compressive strength ranged from 1019 MPa to 767 MPa for alloys with 21ā25% porosities and from 173 MPa to 78 MPa for alloys with 50ā56% porosities. Noted that adding a space holder agent played a more critical role in the mechanical behaviors of the alloys compared to adding niobium. The pores were largely open and exhibited irregular shapes, with uniform size distribution, allowing for cell ingrowth. Histological analysis showed that the alloys studied met the biocompatibility criteria required for orthopaedic biomaterial use.EPSRC Future LiME Hub (EP/N007638/1); PhD studentship, the Republic of Turkey Ministry of National Education
How transcription proceeds in a large artificial heterochromatin in human cells
Heterochromatin is critical for genome integrity, and recent studies have suggested the importance of transcription in heterochromatin for maintaining its silent state. We previously developed a method to generate a large homogeneously staining region (HSR) composed of tandem plasmid sequences in human cells that showed typical heterochromatin characteristics. In this study, we examined transcription in the HSR. We found that transcription of genes downstream to no-inducible SRĪ± promoter was restricted to a few specific points inside the large HSR domain. Furthermore, the HSR localized to either to the surface or to the interior of the nucleolus, where it was more actively transcribed. The perinucleolar or intranucleolar locations were biased to late or early S-phase, and the location depended on either RNA polymerase II/III or I transcription, respectively. Strong activation of the inducible TRE promoter resulted in the reversible loosening of the HSR domain and the appearance of transcripts downstream of not only the TRE promoters, but also the SRĪ± promoters. During this process, detection of HP1Ī± or H3K9Me3 suggested that transcription was activated at many specific points dispersed inside large heterochromatin. The transcriptional rules obtained from studying artificial heterochromatin should be useful for understanding natural heterochromatin
Coronary Artery bypass grafting and/or valvular surgery in patients with previous pneumonectomy
The anatomy and variations of the internal thoracic (internal mammary) artery and implications in autologous breast reconstruction: clinical anatomical study and literature review
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