Analysis of Balmer Profiles of early type stars

The spectral energy distribution (SED) of recent star formation regions is dominated by the more massive and early stars (O and B types). These stars show large and very significant absorption features, the most prominent being the recombination lines of H, HeI and HeII. In particular, the shape of their profiles are very dependent on the luminosity of the star. We have explored the potential use of high resolution profiles to discriminate between different luminosity classes and spectral types, by using profiles of the He and Balmer lines. We have calculated growth curves for each of the lines and their dependence on gravity and effective temperature. We show some of these theoretical growth curves and our preliminary conclusions are analyzed and discussed.Comment: 1 pag, Contribution to the conference "Cosmic Evolution and Galaxy Formation: Structure, Interactions and Feedback", Nov. 1999 (Puebla, Mexico), to be published in ASP. Conf. Series, Eds. J. Franco, E. Terlevich. O. Lopez-Cruz, I. Aretxag

Improvement of effect of water-in-oil microemulsion as an oral delivery system for fexofenadine: in vitro and in vivo studies

Fexofenadine (FEX) has high solubility and low permeability (BCS, Class III). In this work, novel FEX loaded water in oil microemulsion (w/o) was designed to improve bioavailability and compared with Fexofen® syrup in in vitro and in vivo studies. In addition, pharmacokinetic parameters in permeability studies were estimated by using WinNonLin software program. w/o microemulsion system was optimized using a pseudoternary phase diagram, composed of span 80/lutrol F 68 (9.5:0.5 w/w), oleic acide, isopropyl alcohol and water as surfactant mixture; oil and cosurfactant was developed for oral drug delivery. w/o microemulsion systems were characterized by phase behavior, particle size, viscosity and solubilization capacity. In vitro studies were studied using Caco-2 cell monolayer. Pharmacokinetic parameters of w/o microemulsion were investigated in rabbits and compared to Fexofen® syrup. Fexofen® syrup and microemulsion were administered by oral gavage at 6 mg/kg of the same concentration. The experimental results indicated that microemulsion (HLB = 5.53) formed nanometer sized droplets (33.29 ± 1.76) and had good physical stability. This microemulsion increased the oral bioavailability of FEX which was highly water-soluble but fairly impermeable. The relative bioavailability of FEX microemulsion was about 376.76% compared with commercial syrup in rabbits. In vitro experiments were further employed for the enhanced effect of the microemulsion for FEX. These results suggest that novel w/o microemulsion plays an important role in enhancing oral bioavailability of low permeability drugs

Impaired modulation of the cardiac L-type Ca2+ channel activity by ahnak-1 after myocardial infarction

Introduction: The L-type cardiac Ca2+ channel (Cav 1.2) is an important determinant of cardiac repolarization and the main source of activator Ca2+ during excitation-contraction coupling in cardiac cells. Its defective regulation is a major cause of arrhythmias and contractile dysfunction. We have recently shown that the cytoskeletal protein ahnak-1 modulates Ca2+ current through Cav 1.2 channels (ICaL) by interacting with the regulatory beta-subunit of the Cav 1.2 channel and that the genetic variant of ahnak-1 I5483T (previously Ile5236Thr), interferes with the beta-adrenergic stimulation of ICaL. Objective: To extend our study of the I5483T variant to ventricular cardiomyocytes dissociated from remodelled infarcted rat hearts (PMI). Methods: The patch-clamp technique was used to record ICaL from enzymatically dissociated ventricular cardiomyocytes from young (2-month-old) and six-month-old sham-operated and PMI rats. Results: Basal ICaL was increased from 11 ± 0.5 A/F in young cardiomyocytes to 14.6 ± 1.1 A/F and 15.7 ± 1 A/F in sham and PMI cardiomyocytes respectively, while isoprenaline (ISO, 1 µmol/L) further increased ICaL by 101 ± 6%, 109 ± 10% and 104 ± 12% respectively. When cells were intracellularly perfused with a peptide containing the mutated ahnak-1 sequence (10 µmol/L) basal ICaL was increased to 20 ± 1 A/F, 22 ± 2 A/F and 21 ± 2 A/F in young, sham and PMI cardiomyocytes respectively. In these cells ISO increased ICaL by 11 ± 4%, 33 ± 6% and 79 ± 12% respectively. Conclusion: Modulation of ICaL by ahnak-1is impaired by myocardial ischemia and remodelling. Since ahnak-1 and Cav 1.2 channels co-localize in the transverse T-tubule system, remodelling of T-tubules could affect the interaction of ahnak-1 with the regulatory beta subunit of these channels

Combination of polymeric superplasticizers, water repellents and pozzolanic agents to improve air lime-based grouts for historic masonry repair

This paper presents the experimental procedure to develop air lime-based injection grouts including polymeric superplasticizers, a water repellent agent and pozzolanic agents as additives. Research focuses on the development of grouts to improve various characteristics simultaneously combining different additions and admixtures. Aiming to improve the injectability of the grouts, in this study different polymeric superplasticizers were added, namely polycarboxylated-ether derivative (PCE), polynaphthalene sulfonate (PNS) and condensate of melamine-formaldehyde sulfonate (SMFC). Sodium oleate was also used as a water repellent agent to reduce the water absorption. The enhancement of the strength and setting time was intended by using microsilica and metakaolin as pozzolanic mineral additions. Compatibility between the different admixtures and action mechanism of the different polymers were studied by means of zeta potential and adsorption isotherms measurements. Diverse grout mixtures were produced and investigated assessing their injectability, fluidity, stability, compressive strength, hydrophobicity and durability. This research leads to several suitable mixtures produced by using more than one component to enhance efficiency and to provide better performance of grouts. According to the results, the grout composed of air lime, metakaolin, sodium oleate and PCE was found the most effective composition improving the mechanical strength, injectability and hydrophobicity

Early Science with the Large Millimeter Telescope: an energy-driven wind revealed by massive molecular and fast X-ray outflows in the Seyfert Galaxy IRAS 17020+4544

We report on the coexistence of powerful gas outflows observed in millimeter and X-ray data of the Radio-Loud Narrow Line Seyfert 1 Galaxy IRAS 17020+4544. Thanks to the large collecting power of the Large Millimeter Telescope, a prominent line arising from the 12CO(1-0) transition was revealed in recent observations of this source. The complex profile is composed by a narrow double-peak line and a broad wing. While the double-peak structure may be arising in a disk of molecular material, the broad wing is interpreted as the signature of a massive outflow of molecular gas with an approximate bulk velocity of -660 km/s. This molecular wind is likely associated to a multi-component X-ray Ultra-Fast Outflow with velocities reaching up to ~0.1c and column densities in the range 10^{21-23.9} cm^-2 that was reported in the source prior to the LMT observations. The momentum load estimated in the two gas phases indicates that within the observational uncertainties the outflow is consistent with being propagating through the galaxy and sweeping up the gas while conserving its energy. This scenario, which has been often postulated as a viable mechanism of how AGN feedback takes place, has so far been observed only in ULIRGs sources. IRAS 17020+4544 with bolometric and infrared luminosity respectively of 5X10^{44} erg/s and 1.05X10^{11} L_sun appears to be an example of AGN feedback in a NLSy1 Galaxy (a low power AGN). New proprietary multi-wavelength data recently obtained on this source will allow us to corroborate the proposed hypothesis.Comment: Accepted for publication on ApJ Letters, 9 pages, 4 figure

Cardiotrophin-1 promotes a high survival rate in rabbits with lethal fulminant hepatitis of viral origin

Rabbit hemorrhagic disease virus (RHDV) causes lethal fulminant hepatitis closely resembling acute liver failure (ALF) in humans. In this study, we investigated whether cardiotrophin-1 (CT-1), a cytokine with hepatoprotective properties, could attenuate liver damage and prolong survival in virus-induced ALF. Twenty-four rabbits were infected with 2 × 10(4) hemagglutination units of RHDV. Twelve received five doses of CT-1 (100 μg/kg) starting at 12 h postinfection (hpi) (the first three doses every 6 h and then two additional doses at 48 and 72 hpi), while the rest received saline. The animals were analyzed for survival, serum biochemistry, and viral load. Another cohort (n = 22) was infected and treated similarly, but animals were sacrificed at 30 and 36 hpi to analyze liver histology, viral load, and the expression of factors implicated in liver damage and repair. All infected rabbits that received saline died by 60 hpi, while 67% of the CT-1-treated animals survived until the end of the study. Treated animals showed improved liver function and histology, while the viral loads were similar. In the livers of CT-1-treated rabbits we observed reduction of oxidative stress, diminished PARP1/2 and JNK activation, and decreased inflammatory reaction, as reflected by reduced expression of tumor necrosis factor alpha, interleukin-1β, Toll-like receptor 4, VCAM-1, and MMP-9. In addition, CT-1-treated rabbits exhibited marked upregulation of TIMP-1 and increased expression of cytoprotective and proregenerative growth factors, including platelet-derived growth factor B, epidermal growth factor, platelet-derived growth factor receptor β, and c-Met. In conclusion, in a lethal form of acute viral hepatitis, CT-1 increases animal survival by attenuating inflammation and activating cytoprotective mechanisms, thus representing a promising therapy for ALF of viral origin