Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells

Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together, our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism

Germline breast cancer susceptibility genes, tumor characteristics, and survival.

BACKGROUND: Mutations in certain genes are known to increase breast cancer risk. We study the relevance of rare protein-truncating variants (PTVs) that may result in loss-of-function in breast cancer susceptibility genes on tumor characteristics and survival in 8852 breast cancer patients of Asian descent. METHODS: Gene panel sequencing was performed for 34 known or suspected breast cancer predisposition genes, of which nine genes (ATM, BRCA1, BRCA2, CHEK2, PALB2, BARD1, RAD51C, RAD51D, and TP53) were associated with breast cancer risk. Associations between PTV carriership in one or more genes and tumor characteristics were examined using multinomial logistic regression. Ten-year overall survival was estimated using Cox regression models in 6477 breast cancer patients after excluding older patients (≥75years) and stage 0 and IV disease. RESULTS: PTV9genes carriership (n = 690) was significantly associated (p < 0.001) with more aggressive tumor characteristics including high grade (poorly vs well-differentiated, odds ratio [95% confidence interval] 3.48 [2.35-5.17], moderately vs well-differentiated 2.33 [1.56-3.49]), as well as luminal B [HER-] and triple-negative subtypes (vs luminal A 2.15 [1.58-2.92] and 2.85 [2.17-3.73], respectively), adjusted for age at diagnosis, study, and ethnicity. Associations with grade and luminal B [HER2-] subtype remained significant after excluding BRCA1/2 carriers. PTV25genes carriership (n = 289, excluding carriers of the nine genes associated with breast cancer) was not associated with tumor characteristics. However, PTV25genes carriership, but not PTV9genes carriership, was suggested to be associated with worse 10-year overall survival (hazard ratio [CI] 1.63 [1.16-2.28]). CONCLUSIONS: PTV9genes carriership is associated with more aggressive tumors. Variants in other genes might be associated with the survival of breast cancer patients. The finding that PTV carriership is not just associated with higher breast cancer risk, but also more severe and fatal forms of the disease, suggests that genetic testing has the potential to provide additional health information and help healthy individuals make screening decisions

Improving the Performance of Optical Phased Array by Reducing Relative Intensity Noise of Optically Injection-Locked Laser Array

Relative intensity noise (RIN) is an important factor that determines the performance of optical phased arrays (OPA) that are configured using semiconductor lasers as light emission sources. This study proposes a method of improving the optical signal-to-noise ratio (OSNR) of an OPA by reducing the RIN and using high coherence of optically injection-locked (OIL) laser arrays. We numerically demonstrated a laser RIN reduction of 22.7 dB by the OIL laser compared to a free-running laser. We achieved an OPA RIN reduction of 13.2 dB by combining the coherent outputs with the uncorrelated noise of 21 OIL lasers, compared to a single OIL laser RIN. Consequently, we demonstrated an OPA OSNR increase of approximately 13.8 dB based on the OIL-based OPA compared to that of the conventional noise-correlated OPA configuration. Additionally, we confirmed the maintenance of OPA OSNR improvement during OPA operations

Improving the Performance of Optical Phased Array by Reducing Relative Intensity Noise of Optically Injection-Locked Laser Array

Relative intensity noise (RIN) is an important factor that determines the performance of optical phased arrays (OPA) that are configured using semiconductor lasers as light emission sources. This study proposes a method of improving the optical signal-to-noise ratio (OSNR) of an OPA by reducing the RIN and using high coherence of optically injection-locked (OIL) laser arrays. We numerically demonstrated a laser RIN reduction of 22.7 dB by the OIL laser compared to a free-running laser. We achieved an OPA RIN reduction of 13.2 dB by combining the coherent outputs with the uncorrelated noise of 21 OIL lasers, compared to a single OIL laser RIN. Consequently, we demonstrated an OPA OSNR increase of approximately 13.8 dB based on the OIL-based OPA compared to that of the conventional noise-correlated OPA configuration. Additionally, we confirmed the maintenance of OPA OSNR improvement during OPA operations

Characterization of a Functional Hydrogel Layer on a Silicon-Based Grating Waveguide for a Biochemical Sensor

We numerically demonstrated the characteristics of a functional hydrogel layer on a silicon-based grating waveguide for a simple, cost-effective refractive index (RI) biochemical sensor. The RI of the functional hydrogel layer changes when a specific biochemical interaction occurs between the hydrogel-linked receptors and injected ligand molecules. The transmission spectral profile of the grating waveguide shifts depends on the amount of RI change caused by the functional layer. Our characterization includes the effective RI change caused by the thickness, functional volume ratio, and functional strength of the hydrogel layer. The results confirm the feasibility of, and set design rules for, hydrogel-assisted silicon-based grating waveguides

Scaling of resonance frequency for strong injection-locked lasers

It has been shown that strong optical injection locking can significantly enhance the resonance frequency of semiconductor lasers. In this Letter, we describe the trade-off between the maximum resonance frequency enhancement and the quality factor (Q) of the lossless laser cavity and show that the time-bandwidth product (product of photon lifetime and maximum resonance frequency) is equal to one half the square root of the external power injection ratio. The theoretical model agrees well with our experimental data. (C) 2007 Optical Society of America

Frequency response enhancement of optical injection-locked lasers

The modulation response of injection-locked lasers has been carefully analyzed, theoretically and experimentally, with a focus on the strong optical injection regime. We derive closed-form solutions to the relaxation oscillation (resonance) frequency and damping term, as well as the low-frequency damping term, and discuss design rules for maximizing resonance frequency and broadband performance. A phasor model is described in order to better explain the enhancement of the resonance frequency. Experimental curves match closely to theory. Record resonance frequency of 72 GHz and broadband results are shown

Theoretical Demonstration of Security Improvement of Optical Phased Array Based on Optically Injection-Locked Lasers

The high security of optical phased array (OPA) signals is an important requirement for OPA-based optical wireless communication (OWC). We propose a method for improving the security of OPA-based OWC systems using optically injection-locked (OIL) semiconductor lasers. We theoretically demonstrate the amplitude and phase modulation of OIL-OPA elements by controlling the injection-locking parameters of the OIL lasers. When a Taylor window function is applied as the amplitude profile of the OPA transmitter, the sidelobe level decreases by 22 dB and the unsecured distance reduces 10 times compared to the case without the Taylor window function. In addition, the unsecured area factor becomes 0.8%