832 research outputs found
The Transition State in a Noisy Environment
Transition State Theory overestimates reaction rates in solution because
conventional dividing surfaces between reagents and products are crossed many
times by the same reactive trajectory. We describe a recipe for constructing a
time-dependent dividing surface free of such recrossings in the presence of
noise. The no-recrossing limit of Transition State Theory thus becomes
generally available for the description of reactions in a fluctuating
environment
Simultaneous multiwavelength observations of V404 Cygni during its 2015 June outburst decay strengthen the case for an extremely energetic jet-base
We present results of multiband optical photometry of the black hole X-ray
binary system V404 Cygni obtained using Wheaton College Observatory's 0.3m
telescope, along with strictly simultaneous INTEGRAL and Swift observations
during 2015 June 25.15--26.33 UT, and 2015 June 27.10--27.34 UT. These
observations were made during the 2015 June outburst of the source when it was
going through an epoch of violent activity in all wavelengths ranging from
radio to -rays. The multiwavelength variability timescale favors a
compact emission region, most likely originating in a jet outflow, for both
observing epochs presented in this work. The simultaneous INTEGRAL/Imager on
Board the Integral Satellite (IBIS) 20--40 keV light curve obtained during the
June 27 observing run correlates very strongly with the optical light curve,
with no detectable delay between the optical bands as well as between the
optical and hard X-rays. The average slope of the dereddened spectral energy
distribution was roughly flat between the - and -bands during the June
27 run, even though the optical and X-ray flux varied by 25 during
the run, ruling out an irradiation origin for the optical and suggesting that
the optically thick to optically thin jet synchrotron break during the
observations was at a frequency larger than that of -band, which is quite
extreme for X-ray binaries. These observations suggest that the optical
emission originated very close to the base of the jet. A strong H
emission line, probably originating in a quasi-spherical nebula around the
source, also contributes significantly in the -band. Our data, in
conjunction with contemporaneous data at other wavelengths presented by other
groups, strongly suggest that the jet-base was extremely compact and energetic
during this phase of the outburst.Comment: 15 pages, 2 tables, 5 figures. Accepted for publication in Ap
Complexity of murine cardiomyocyte miRNA biogenesis, sequence variant expression and function
microRNAs (miRNAs) are critical to heart development and disease. Emerging research indicates that regulated precursor processing can give rise to an unexpected diversity of miRNA variants. We subjected small RNA from murine HL-1 cardiomyocyte cells to next generation sequencing to investigate the relevance of such diversity to cardiac biology. ∼40 million tags were mapped to known miRNA hairpin sequences as deposited in miRBase version 16, calling 403 generic miRNAs as appreciably expressed. Hairpin arm bias broadly agreed with miRBase annotation, although 44 miR* were unexpectedly abundant (>20% of tags); conversely, 33 -5p/-3p annotated hairpins were asymmetrically expressed. Overall, variability was infrequent at the 5' start but common at the 3' end of miRNAs (5.2% and 52.3% of tags, respectively). Nevertheless, 105 miRNAs showed marked 5' isomiR expression (>20% of tags). Among these was miR-133a, a miRNA with important cardiac functions, and we demonstrated differential mRNA targeting by two of its prevalent 5' isomiRs. Analyses of miRNA termini and base-pairing patterns around Drosha and Dicer cleavage regions confirmed the known bias towards uridine at the 5' most position of miRNAs, as well as supporting the thermodynamic asymmetry rule for miRNA strand selection and a role for local structural distortions in fine tuning miRNA processing. We further recorded appreciable expression of 5 novel miR*, 38 extreme variants and 8 antisense miRNAs. Analysis of genome-mapped tags revealed 147 novel candidate miRNAs. In summary, we revealed pronounced sequence diversity among cardiomyocyte miRNAs, knowledge of which will underpin future research into the mechanisms involved in miRNA biogenesis and, importantly, cardiac function, disease and therapy.This work was supported by by the Victor Chang Cardiac Research Institute and grants 573726, 573731 and 514904 from the National Health & Medical
Research Council awarded to TP
Interaction of Dendritic Cells with Skin Endothelium: A New Perspective on Immunosurveillance
The goal of this study was to determine the mechanisms by which dendritic cells (DCs) in blood could interact with endothelium, a prerequisite to extravasation into tissues. Our results indicate that DCs express both HECA-452–reactive and nonreactive isoforms of P-selectin glycoprotein ligand 1 (PSGL-1) and can tether and roll efficiently on E- and P-selectin under flow conditions in vitro. Freshly isolated blood DCs were further observed to roll continuously along noninflamed murine dermal endothelium in vivo. This interaction is strictly dependent on endothelial selectins, as shown by experiments with blocking antibodies and with E- and P-selectin–deficient mice. We hypothesize that DCs in blood are constitutively poised at the interface of blood and skin, ready to extravasate upon induction of inflammation, and we showed that cutaneous inflammation results in a rapid recruitment of DCs from the blood to tissues. We propose that this is an important and previously unappreciated element of immunosurveillance
Multiwavelength Observations of A0620-00 in Quiescence
[Abridged.] We present multiwavelength observations of the black hole binary
system, A0620-00. Using the Cosmic Origins Spectrograph on the Hubble Space
Telescope, we have obtained the first FUV spectrum of A0620-00. The observed
spectrum is flat in the FUV and very faint (with continuum fluxes \simeq 1e -
17 ergs/cm^2/s/A). We compiled the dereddened, broadband spectral energy
distribution of A0620-00 and compared it to previous SEDs as well as
theoretical models. The SEDs show that the source varies at all wavelengths for
which we have multiple samples. Contrary to previous observations, the
optical-UV spectrum does not continue to drop to shorter wavelengths, but
instead shows a recovery and an increasingly blue spectrum in the FUV. We
created an optical-UV spectrum of A0620-00 with the donor star contribution
removed. The non-stellar spectrum peaks at \simeq3000 {\deg}A. The peak can be
fit with a T=10,000 K blackbody with a small emitting area, probably
originating in the hot spot where the accretion stream impacts the outer disk.
However, one or more components in addition to the blackbody are needed to fit
the FUV upturn and the red optical fluxes in the optical-UV spectrum. By
comparing the mass accretion rate determined from the hot spot luminosity to
the mean accretion rate inferred from the outburst history, we find that the
latter is an order of magnitude smaller than the former, indicating that
\sim90% of the accreted mass must be lost from the system if the predictions of
the disk instability model and the estimated interoutburst interval are
correct. The mass accretion rate at the hot spot is 10^5 the accretion rate at
the black hole inferred from the X-ray luminosity. To reconcile these requires
that outflows carry away virtually all of the accreted mass, a very low rate of
mass transfer from the outer cold disk into the inner hot region, and/or
radiatively inefficient accretion.Comment: ApJ, accepte
Human liver glycogen phosphorylase inhibitors bind at a new allosteric site
AbstractBackground: Glycogen phosphorylases catalyze the breakdown of glycogen to glucose-1-phosphate for glycolysis. Maintaining control of blood glucose levels is critical in minimizing the debilitating effects of diabetes, making liver glycogen phosphorylase a potential therapeutic target.Results: The binding site in human liver glycogen phosphorylase (HLGP) for a class of promising antidiabetic agents was identified crystallographically. The site is novel and functions allosterically by stabilizing the inactive conformation of HLGP. The initial view of the complex revealed key structural information and inspired the design of a new class of inhibitors which bind with nanomolar affinity and whose crystal structure is also described.Conclusions: We have identified the binding site of a new class of allosteric HLGP inhibitors. The crystal structure revealed the details of inhibitor binding, led to the design of a new class of compounds, and should accelerate efforts to develop therapeutically relevant molecules for the treatment of diabetes
Developing institutional capacity for reproductive health in humanitarian settings: A descriptive study
© 2015 Tran et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Introduction. Institutions play a central role in advancing the field of reproductive health in humanitarian settings (RHHS), yet little is known about organizational capacity to deliver RHHS and how this has developed over the past decade. This study aimed to document the current institutional experiences and capacities related to RHHS. Materials and Methods. Descriptive study using an online questionnaire tool. Results. Respondents represented 82 institutions from 48 countries, of which two-thirds originated from low-and middle-income countries. RHHS work was found not to be restricted to humanitarian agencies (25%), but was also embraced by development organizations (25%) and institutions with dual humanitarian and development mandates (50%). Agencies reported working with refugees (81%), internally-displaced (87%) and stateless persons (20%), in camp-based settings (78%), and in urban (83%) and rural settings (78%). Sixtyeight percent of represented institutions indicated having an RHHS-related policy, 79% an accountability mechanism including humanitarian work, and 90% formal partnerships with other institutions. Seventy-three percent reported routinely appointing RH focal points to ensure coordination of RHHS implementation. There was reported progress in RHHSrelated disaster risk reduction (DRR), emergency management and coordination, delivery of the Minimum Initial Services Package (MISP) for RH, comprehensive RH services in post-crisis/recovery situations, gender mainstreaming, and community-based programming. Other reported institutional areas of work included capacity development, program delivery, advocacy/policy work, followed by research and donor activities. Except for abortion-related services, respondents cited improved efforts in advocacy, capacity development and technical support in their institutions for RHHS to address clinical services, including maternal and newborn health, sexual violence prevention and response, HIV prevention, management of sexually-transmitted infections, adolescent RH, and family planning. Approximately half of participants reported that their institutions had experienced an increase in dedicated budget and staff for RHHS, a fifth no change, and 1 in 10 a decrease. The Interagency RH Kits were reportedly the most commonly used supplies to support RHHS implementation. Conclusion. The results suggest overall growth in institutional capacity in RHHS over the past decade, indicating that the field has matured and expanded from crisis response to include RHHS into DRR and other elements of the emergency management cycle. It is critical to consolidate the progress to date, address gaps, and sustain momentum
SPH Simulations of Negative (Nodal) Superhumps: A Parametric Study
Negative superhumps in cataclysmic variable systems result when the accretion
disc is tilted with respect to the orbital plane. The line of nodes of the
tilted disc precesses slowly in the retrograde direction, resulting in a
photometric signal with a period slightly less than the orbital period. We use
the method of smoothed particle hydrodynamics to simulate a series of models of
differing mass ratio and effective viscosity to determine the retrograde
precession period and superhump period deficit as a function of
system mass ratio . We tabulate our results and present fits to both
and versus , as well as compare the
numerical results with those compiled from the literature of negative superhump
observations. One surprising is that while we find negative superhumps most
clearly in simulations with an accretion stream present, we also find evidence
for negative superhumps in simulations in which we shut off the mass transfer
stream completely, indicating that the origin of the photometric signal is more
complicated than previously believed.Comment: 14 pages, 15 figures. Accepted for publication in MNRA
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