Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

VizieR Online Data Catalog Optical and IR photometry of OGLE-2017-BLG-0406 (Hirao+, 2020)

The microlensing event OGLE-2017-BLG-0406 was first discovered on March 27 (HJD'=HJD-2450000=7839) by the Optical Gravitational Lensing Experiment (OGLE) collaboration. The event lies in the OGLE-IV field BLG506, and the observations were conducted at the cadence of once per hour by using the 1.3m Warsaw telescope located at Las Campanas Observatory in Chile. The Microlensing Observations in Astrophysics (MOA) group independently discovered this event on May 5 (HJD'=7879) by using the MOA alert system. MOA observed this event with 15 minutes cadence by using MOA-II telescope at Mt. John University Observatory in New Zealand. The event was also independently discovered by the Korean Microlensing Network (KMTNet) survey using its post-season event finder. KMTNet observes toward the Galactic bulge by using three 1.6m telescopes at the Cerro Tololo Inter-American Observatory in Chile (CTIO: KMT-C), and the South African Astronomical Observatory in South Africa (SAAO: KMT-S). Because this event was in an overlapping region between two fields (KMTNet BLG02 and BLG42), the observations were conducted at a 15minute cadence. On June 2 (HJD'=7907), the Microlensing Follow-up Network (μFUN) collaboration, the Microlensing Network for the Detection of Small Terrestrial Exoplanet (MiNDSTEp) collaboration and Las Cumbres Observatory (LCO) global network of telescope collaboration started high-cadence follow-up observations. μFUN used the following telescopes: the 1.3m CTIO telescope in Chile, the 0.41m Auckland telescope and the 0.36m Farm Cove telescope in New Zealand, and the 0.30m Perth Exoplanet Survey Telescope (PEST), and the 0.25m Craigie telescope in Australia. MiNDSTEp used the 1.54m Danish Telescope at La Silla Observatory in Chile. LCO used the 1.0m telescopes at CTIO in Chile and at SSO in Australia. We also obtained three near-infrared images taken at different epochs (HJD'~7911, 7918 and 7942). The observations were made with SIRIUS, a simultaneous imager in J, H, and KS bands, covering an area 7.7x.7 arcmin2 with a pixel scale of 0.45" on the 1.4m InfraRed Survey Facility (IRSF) telescope at SAAO. OGLE-2017-BLG-0406 was observed by the Spitzer space telescope with the 3.6μm (L-band) channel of the IRAC camera. Spitzer started to observe this event on June 26 (HJD'=7931). (17 data files)...

VizieR Online Data Catalog Optical and IR photometry of OGLE-2017-BLG-0406 (Hirao+, 2020)

The microlensing event OGLE-2017-BLG-0406 was first discovered on March 27 (HJD'=HJD-2450000=7839) by the Optical Gravitational Lensing Experiment (OGLE) collaboration. The event lies in the OGLE-IV field BLG506, and the observations were conducted at the cadence of once per hour by using the 1.3m Warsaw telescope located at Las Campanas Observatory in Chile. The Microlensing Observations in Astrophysics (MOA) group independently discovered this event on May 5 (HJD'=7879) by using the MOA alert system. MOA observed this event with 15 minutes cadence by using MOA-II telescope at Mt. John University Observatory in New Zealand. The event was also independently discovered by the Korean Microlensing Network (KMTNet) survey using its post-season event finder. KMTNet observes toward the Galactic bulge by using three 1.6m telescopes at the Cerro Tololo Inter-American Observatory in Chile (CTIO: KMT-C), and the South African Astronomical Observatory in South Africa (SAAO: KMT-S). Because this event was in an overlapping region between two fields (KMTNet BLG02 and BLG42), the observations were conducted at a 15minute cadence. On June 2 (HJD'=7907), the Microlensing Follow-up Network (μFUN) collaboration, the Microlensing Network for the Detection of Small Terrestrial Exoplanet (MiNDSTEp) collaboration and Las Cumbres Observatory (LCO) global network of telescope collaboration started high-cadence follow-up observations. μFUN used the following telescopes: the 1.3m CTIO telescope in Chile, the 0.41m Auckland telescope and the 0.36m Farm Cove telescope in New Zealand, and the 0.30m Perth Exoplanet Survey Telescope (PEST), and the 0.25m Craigie telescope in Australia. MiNDSTEp used the 1.54m Danish Telescope at La Silla Observatory in Chile. LCO used the 1.0m telescopes at CTIO in Chile and at SSO in Australia. We also obtained three near-infrared images taken at different epochs (HJD'~7911, 7918 and 7942). The observations were made with SIRIUS, a simultaneous imager in J, H, and KS bands, covering an area 7.7x.7 arcmin2 with a pixel scale of 0.45" on the 1.4m InfraRed Survey Facility (IRSF) telescope at SAAO. OGLE-2017-BLG-0406 was observed by the Spitzer space telescope with the 3.6μm (L-band) channel of the IRAC camera. Spitzer started to observe this event on June 26 (HJD'=7931). (17 data files)...

Analysis of Outcomes in Ischemic vs Nonischemic Cardiomyopathy in Patients With Atrial Fibrillation A Report From the GARFIELD-AF Registry

IMPORTANCE Congestive heart failure (CHF) is commonly associated with nonvalvular atrial fibrillation (AF), and their combination may affect treatment strategies and outcomes

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

non present