In vivo pathogenicity of hydropericardium hepatitis syndrome (Angara disease)

This study was conducted on 175 one day old broiler chicks in order to observe in vivo pathogenicity of hydropericardium hepatitis syndrome (Angara disease). Chicks were grouped into A (25 chicks; reared at the Poultry Research institute (PRI), Rawalpindi) and B groups (150 chicks; reared at National Veterinary Laboratories (NVL) Islamabad in poultry rearing units). Feed and water were provided adlibitum to all the birds in both groups. The LD50 (lethal dose 50) of the virus was determined at the age of 26 days of broiler chickens divided into sub-groups a, b, c, d and e (each having 5 birds). The LD50 titre of the viral suspension (10%) was prepared from liver extract and determined as 10-2.5 per ml. During the study, potency of the vaccines was determined by vaccinating 150 chicks of sub-group k, l, m, n and o (each having 30 birds) with a dose of 0.2 ml. The vaccinated and non-vaccinated chicks of sub-groups were challenged with viral dose of 2 ml at day 17 post-vaccination to know the protection potency of the vaccines. No chick showed clinical manifestation of disease up to five days post challenge. On the 6th day post challenge, all the chicks were slaughtered and subjected to postmortem. Some of the chicks showed the lesions of hydropericarium. Histopathological findings of liver of all sub-groups revealed different stages of necrosis, cloudy swelling, liquefactive necrosis, cytoplasmic blabing, fatty degeneration and intranuclear inclusion bodies of virus.Key words: In vivo pathogenicity, hydropericardium hepatitis syndrome, Angara disease

Assessing cloud QoS predictions using OWA in neural network methods

Quality of Service (QoS) is the key parameter to measure the overall performance of service-oriented applications. In a myriad of web services, the QoS data has multiple highly sparse and enormous dimensions. It is a great challenge to reduce computational complexity by reducing data dimensions without losing information to predict QoS for future intervals. This paper uses an Induced Ordered Weighted Average (IOWA) layer in the prediction layer to lessen the size of a dataset and analyse the prediction accuracy of cloud QoS data. The approach enables stakeholders to manage extensive QoS data better and handle complex nonlinear predictions. The paper evaluates the cloud QoS prediction using an IOWA operator with nine neural network methods—Cascade-forward backpropagation, Elman backpropagation, Feedforward backpropagation, Generalised regression, NARX, Layer recurrent, LSTM, GRU and LSTM-GRU. The paper compares results using RMSE, MAE, and MAPE to measure prediction accuracy as a benchmark. A total of 2016 QoS data are extracted from Amazon EC2 US-West instance to predict future 96 intervals. The analysis results show that the approach significantly decreases the data size by 66%, from 2016 to 672 records with improved or equal accuracy. The case study demonstrates the approach's effectiveness while handling complexity, reducing data dimension with better prediction accuracy

Analysis of chemical constituents in medicinal plants of selected districts of Pakhtoonkhwa, Pakistan

Alkaloids, flavonoids and saponins were determined in the Teraxacam officinale, Cichorium intybus and Figonia tritica. Quantative determinations of crude alkaloids, flavonoids and saponins (g kg-1) and their percentages (%) were determined in C. intybus, T. officinale and F. critica collected from Kohat, Mardan, Nowshera and Peshawar regions. Thin layer chromatography (TLC) study of the alkaloids, flavonoids and saponins were carried out with different solvent systems and color of the spot and Rf value of each constituent was determined. Among the samples of C. intybus, relatively higher contents of alkaloids (14 g kg-1) were found in the sample collected from Mardan region, followed by the sample gathered from Nowshera which is equal to 13.8g kg-1. Higher contents of flavonoids were found in C. intybus collected from Kohat region which was 23.48 g kg-1. The other remaining samples of C. intybus ranged in the decreasing order of 19.7, 17.2 and 10.0 g kg-1 for the ones collected from Nowshera, Mardan and Peshawar, respectively. The overall saponin contents remained almost low as compared to the alkaloid and flavonoid contents.Key words: Alkaloids, flavonoids, saponins, TLC study, medicinal plants

Heavy metals and inorganic constituents in medicinal plants of selected Districts of Khyber Pakhtoonkhwa, Pakistan

Heavy metals such as Cr, Fe, Zn, Mn, Ni, Pb, Cu and Cd, and inorganic ions like HCO3-, CO32-, Ca2+, Mg2+, Cl-, Na+, SO42-, NO3-, Fe2+ and F- were investigated in medicinally important plants: Taraxacam officinale, Cichorium intybus and Figonia critica, applying atomic absorption spectrophotometer techniques. In the studied herbs, there were variable amounts of heavy metals and inorganic ions. The purpose of this study was to standardize heavy metals contamination in various indigenous medicinal plants and to create awareness among the public regarding its safe use at collection areas (containing high level of heavy metals) and their adverse health effects.Key words: Medicinal plants, inorganic constituents, heavy metals, atomic absorption spectrophotometer

Gangrenous cholecystitis in an asymptomatic patient found during an elective laparoscopic cholecystectomy: a case report

<p>Abstract</p> <p>Introduction</p> <p>Gangrenous cholecystitis is a severe complication of acute cholecystitis. We present an unusual case of gangrenous cholecystitis which was totally asymptomatic, with normal pre-operative parameters, and was discovered incidentally during a laparoscopic cholecystectomy. We have not found any similar cases in the published literature.</p> <p>Case presentation</p> <p>A 79-year-old British Caucasian man presented initially with acute cholecystitis which responded to conservative management. After six weeks he was asymptomatic and had normal blood parameters. An elective laparoscopic cholecystectomy was performed and our patient was found to have a totally gangrenous gall bladder.</p> <p>Conclusion</p> <p>It is important to keep a high index of suspicion for the diagnosis of gangrenous cholecystitis in order to avoid potentially serious complications.</p

Phytochemical analysis of selected medicinal plants

Four medicinal plants including Ranunculus arvensis, Equisetum ravens, Carathamus lanatus and Fagonia critica were used for the study. All the plants were biologically active and were used for different types of ailments. Keeping in view their importance, this work was carried out to investigate the quantitative determination of their crude phytochemicals, vitamins and protein contents. The quantitative determination of crude phytochemicals (alkaloids, total phenols, flavonoids and saponins) vitamins (riboflavin, vitamin C, niacin and pectin) and protein were determined in the aforementioned herbs. The phytochemicals including alkaloids, total phenols, flavonoids and saponins were determined quantitatively using literature methods. Vitamins were measured using a UV/ visible spectrophotometer (UV- 1601 Shamidzu) and the protein was determined by the Micro KJeldahl’s method (Horwitz et al., 2000). The studied plants showed variable amounts of phytochemicals, vitamins and protein contents. The study is very important, in that it intended to show the contents of the studied medicinal herbs and also provide a scientific data base line which is of particular importance for the local practioners as well as for the local people using these herbs for a variety of body disorders.Key word: Phytochemical analysis, medicinal plants, Pakistan

A Feynman integral in Lifshitz-point and Lorentz-violating theories in R^D ⨁ R<i>^m</i>

We evaluate a 1-loop, 2-point, massless Feynman integral ID,m(p,q) relevant for perturbative field theoretic calculations in strongly anisotropic d=D+m dimensional spaces given by the direct sum RD ⨁ Rm . Our results are valid in the whole convergence region of the integral for generic (noninteger) codimensions D and m. We obtain series expansions of ID,m(p,q) in terms of powers of the variable X:=4p2/q4, where p=|p|, q=|q|, p Є RD, q Є Rm, and in terms of generalised hypergeometric functions 3F2(−X), when X&lt;1. These are subsequently analytically continued to the complementary region X≥1. The asymptotic expansion in inverse powers of X1/2 is derived. The correctness of the results is supported by agreement with previously known special cases and extensive numerical calculations

Analysis of associated risk factors among recurrent cutaneous leishmaniasis patients: A cross-sectional study in Khyber Pakhtunkhwa, Pakistan

Background Leishmaniasis is the second and fourth highest cause of mortality and morbidity respectively among all tropical diseases. Recurrence in the onset of leishmaniasis is a major problem that needs to be addressed to reduce the case fatality rate and ensure timely clinical intervention. Here we are investigating the association of risk factors with recurrent cutaneous leishmaniasis to address this issue. Material and methods Patients received by Nasser Ullah Khan Babar Hospital in Peshawar, Pakistan from March 2019 to July 2020 were enrolled in this study. Those patients who developed symptoms after completion of treatment were included in Group-A while those who had atypical scars like leishmaniasis but were negative for cutaneous leishmaniasis were included in the comparison group tagged as Group B. All those individuals who had completed six weeks of treatment for CL but had normal complete blood counts (CBC) were included to avoid other underlying immunological pathologies, while we excluded those participants who had co-morbidities like diabetes, liver disease, cardiac disease, and pregnant and lactating women through their history Association was tested between Group-A and Group-B with other explanatory variables through chi-square test. The regression model was proposed to determine the predictors. Result A total of 48 participants of both sexes were included in the study with a mean age of 32.2 ± 15.10. The data suggest that females are overrepresented among the patients with recurrent leishmaniasis [21(53.8 %,); p = 0.07]. Compared to patients; healthy participants had a higher proportion of adults (19–59 years) versus adolescents (13–18 years) [26(66.7 %) vs 07(17.9), p = 0.004]. Multivariate logistic regression analysis shows that females are 2.1 times more prone to infections among cases as compared to healthy individuals [unadjusted OR 2.20, 95 % confidence interval (CI) 1.5–10.6, p = 0.02; adjusted OR 2.1, 95 % CI 1.50–10.69, p = 0.02]. We propose that patients receiving intradermal were less likely to be infected as compared to those receiving intralesional injections [unadjusted OR 0.07.0, 95 % confidence interval (CI) 1.18–3.37, p = 0.03; adjusted OR 0.06, 95 % CI 1.18–3.38, p = 0.03]. Conclusion Old age (adults) and sex (females) were the strongest predictors to be associated with recurrent leishmaniasis. Similarly, the choice of intradermal as compared to intralesional injection and the prolonged treatment duration were strongly associated with greater chances of recurrence

Extensive Copy-Number Variation of Young Genes across Stickleback Populations

MM received funding from the Max Planck innovation funds for this project. PGDF was supported by a Marie Curie European Reintegration Grant (proposal nr 270891). CE was supported by German Science Foundation grants (DFG, EI 841/4-1 and EI 841/6-1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript