3 research outputs found

    Sistema de seguimiento y an谩lisis de la calidad del agua para consumo humano mediante el estudio de la respuesta comportamental en peces expuestos a sustancias t贸xicas

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    Behavioral alterations can be assessed as variables for sublethal聽toxicity tests, and serve as a tool for environmental risk assessment and analysis of toxicological impact. In order to investigate聽contaminant-induced behavioral alterations in fish, a video analysis聽system was designed to obtain relevant behavioral variables. Data聽from fish exposed to a reference toxicant, organophosphorus pesticide chlorpyrifos, are presented to exemplify alterations in fish behavior associated with exposure to this pesticide. The developed聽system provides valuable information on parameters associated聽with fish behavior and can be used to identify characteristic behavioral responses to a variety of toxicants and assist in risk assessment.La obtenci贸n de variables relacionadas con cambios聽comportamentales en los estudios sobre los efectos subletales de聽sustancias t贸xicas en animales, constituyen una herramienta聽fundamental para la evaluaci贸n del riesgo ambiental y el an谩lisis聽del impacto de sustancias t贸xicas. En el presente trabajo se聽presenta un sistema de seguimiento y an谩lisis mediante t茅cnicas聽de visi贸n artificial que permite cuantificar alteraciones en el聽comportamiento en peces expuestos a concentraciones subletales聽de sustancias t贸xicas. El sistema permite la obtenci贸n de una serie聽de variables comportamentales a partir de un grupo de peces聽expuestos a una sustancia t贸xica, en este caso el insecticida聽organofosforado clorpirifos, que posteriormente son analizadas聽para comprobar su desviaci贸n frente a un grupo control. Los聽resultados obtenidos muestran como las variables registradas聽proporcionan informaci贸n muy valiosa sobre el comportamiento en聽peces y constatan que dicho sistema puede ser utilizado para聽caracterizar las respuestas comportamentales frente a la聽exposici贸n de sustancias t贸xicas y en la evaluaci贸n del riesgo聽ambiental

    Bioactive characterization of Persea americana Mill. by-products: A rich source of inherent antioxidants

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    [EN] Avocado (Persea americana Mill.) is a worldwide consumed fruit, with great interest for cosmetic and pharmaceutical industries; however, 30% of avocado fruits are bio-wastes (peels and kernels), converting them into a potential source of bioactive compounds, such as phenolic compounds. Therefore, the hydroethanolic extracts of peels and kernels of Persea america Mill. var. Hass were analysed regarding their individual phenolic profile by HPLC-DAD/ESI-MS and correlated with their antioxidant, antimicrobial and cytotoxic activities. Avocado by-products presented a very distinct phenolic profile, presenting higher concentration in peels (227.9 mg/g of extract for total phenolic content), mainly in (epi)catechin derivatives (175 mg/g of extract), followed by chlorogenic derivatives (42.9 mg/g of extract). In this study hydrophilic and lipophilic antioxidant assays were performed together for the first time in P. americana by-products, and although kernels showed a great antioxidant potential (EC50 values ranging from 18.1 to 276 mu g/mL), peels presented the highest potential (EC50 ranging from 11.7 to 152 mu g/mL), mainly due to the presence of phenolic compounds, and an overall better performance in the antibacterial assays. Further studies needs to be conducted to better understand the correlation between the presence of phenolic compounds and bioactivities, however, the main objective is to implement these biocompounds in different products and industries, due to results obtained, P. americana peels could be a great alternative in the substitution of synthetic antioxidants.The authors are grateful to the Foundation for Science and Technology(FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UID/AGR/00690/2013) and L. Barros contract. The authors would like to thank the Interreg Espana-Portugal for financial support through the project 0377_Iberphenol_6_E. B. Melgar thanks CONACyT for his grant (No. 329930). The authors are also grateful to the Serbian Ministry of Education, Science and Technological Development, grant number 173032 for financial support.Melgar-Casta帽eda, B.; Dias, MI.; Ciric, A.; Sokovic, M.; Garcia-Castello, EM.; Rodr铆guez L贸pez, AD.; Barros, L.... (2018). Bioactive characterization of Persea americana Mill. by-products: A rich source of inherent antioxidants. Industrial Crops and Products. 111:212-218. https://doi.org/10.1016/j.indcrop.2017.10.024S21221811

    Water quality monitoring and analysis system through study of fish behavioral response to toxic compounds

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    La obtenci贸n de variables relacionadas con cambios comportamentales en los estudios sobre los efectos subletales de sustancias t贸xicas en animales, constituyen una herramienta fundamental para la evaluaci贸n del riesgo ambiental y el an谩lisis del impacto de sustancias t贸xicas. En el presente trabajo se presenta un sistema de seguimiento y an谩lisis mediante t茅cnicas de visi贸n artificial que permite cuantificar alteraciones en el comportamiento en peces expuestos a concentraciones subletales de sustancias t贸xicas. El sistema permite la obtenci贸n de una serie de variables comportamentales a partir de un grupo de peces expuestos a una sustancia t贸xica, en este caso el insecticida organofosforado clorpirifos, que posteriormente son analizadas para comprobar su desviaci贸n frente a un grupo control. Los resultados obtenidos muestran como las variables registradas proporcionan informaci贸n muy valiosa sobre el comportamiento en peces y constatan que dicho sistema puede ser utilizado para caracterizar las respuestas comportamentales frente a la exposici贸n de sustancias t贸xicas y en la evaluaci贸n del riesgo ambiental
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