A solid state 94 GHz FMCW Doppler radar demonstrator for cloud profiling

We present the design and characterization of a ground-based, zenith-pointing, 94 GHz FMCW Doppler radar demonstrator for cloud profiling. The radar uses an all solid-state and relatively simple homodyne architecture and two, low sidelobe 0.5 m diameter Fresnel zone plate antennas to reduce system costs. The low-phase noise, coherent radar employs a direct digital synthesis (DDS) chip for highly linear chirp generation. The design will be able to leverage ongoing future improvements in mm-wave low noise and power amplifier technology to maximize sensitivity. Once the radar is installed in a rooftop location, the processor will perform real-time range-Doppler measurements with averaging, to yield target velocity spectra as a function of altitude.Publisher PD

High Gaussicity feedhorns for sub-/ millimeter wave applications

In feedhorn design, the power coupling to the fundamental free-space LG00 mode, or Gaussicity, is a good proxy for high performance, particularly the sidelobe and cross-polar levels and the near-field behavior. Gaussicity can be maximized by ensuring that the first few horn modes reach the aperture with the appropriate phase and amplitude relationship. We present two feedhorn designs for which the Gaussicity was maximized in order to achieve high performance. The first is a 94 GHz corrugated horn with a tanh-linear profile, manufactured by electroforming, which achieves a Gaussicity of 99.92% at band center and sidelobes at the -60 dB level. The second is a 340 GHz smooth-walled spline horn which achieves a Gaussicity of >99.2% over a 10% bandwidth, sidelobes below -30 dB and excellent near-field behavior. This design has been successfully fabricated in E-plane split block suitable for low volume manufacture, for example for imaging arrays.Postprin

Optimization and measurement of a smoothly profiled horn for a W-band gyro-TWA

In this paper, a smoothly profiled horn was designed and manufactured to replace a corrugated output horn for a W-band gyro-TWA with improved ultra-high vacuum compatibility. It was optimized for high vacuum integrity, low reflection, high fundamental Gaussian mode content, low side lobe levels and high directivity over the frequency bandwidth of 90 - 100 GHz. Over this operating frequency band the reflectivity was better than -37 dB and the coupling to a fundamental Gaussian mode was above 97%. The far field pattern showed a directivity of approximately 27 dB in the measurement with side lobes lower than -30 dB

Low Metallicity Indicates that the Hypervelocity Star HE 0437-5439 was Ejected from the LMC

We measure the metallicity of the unusual hypervelocity star HE 0437-5439 from high resolution spectroscopy to be half-solar. We determine a spectral type of B2 IV-III for the star and derive an effective temperature Teff= 21,500 +/- 1,000 K and a surface gravity log(g) = 3.7 +/- 0.2 (cgs). We also present BV time series photometry and find the star to be non-variable at the 0.02 mag level. We refine the magnitude of the hypervelocity star to V=16.36+/-0.04 mag, with a color B-V=-0.23+/-0.03 mag, confirming its early-type nature. Our metallicity result establishes the origin of HE 0437-5439 in the Large Magellanic Cloud and implies the existence of a massive black hole somewhere in this galaxy.Comment: 12 pages, 3 figures, 1 table; accepted to ApJL. Added 2 coauthors & full abundance analysis. Results are strengthene

Towards increased concentration sensitivity for continuous wave EPR investigations of spin-labeled biological macromolecules at high fields

This work was performed at the National High Magnetic Field Laboratory (NHMFL), which is supported by the National Science Foundation (DMR-1157490) and the State of Florida. L.S. acknowledges support from the National Institutes of Health (AI091693) and the NHMFL User Collaboration Grants Program (Award No. 5080). G.E.F. acknowledges support from the National Science Foundation (MCB-1329467) and the National Institutes of Health (GM105409 and S10RR031603). S.H. acknowledges support from the National Science Foundation (DMR-1309463). J.M.E acknowledges support from the National Science Foundation (DGE-0802270).High-field, high-frequency electron paramagnetic resonance (EPR) spectroscopy at W- (∼95 GHz) and D-band (∼140 GHz) is important for investigating the conformational dynamics of flexible biological macromolecules because this frequency range has increased spectral sensitivity to nitroxide motion over the 100 ps to 2 ns regime. However, low concentration sensitivity remains a roadblock for studying aqueous samples at high magnetic fields. Here, we examine the sensitivity of a non-resonant thin-layer cylindrical sample holder, coupled to a quasi-optical induction-mode W-band EPR spectrometer (HiPER), for continuous wave (CW) EPR analyses of: (i) the aqueous nitroxide standard, TEMPO; (ii) the unstructured to α-helical transition of a model IDP protein; and (iii) the base-stacking transition in a kink-turn motif of a large 232 nt RNA. For sample volumes of ∼50 μL, concentration sensitivities of 2-20 μM were achieved, representing a ∼10-fold enhancement compared to a cylindrical TE011 resonator on a commercial Bruker W-band spectrometer. These results therefore highlight the sensitivity of the thin-layer sample holders employed in HiPER for spin-labeling studies of biological macromolecules at high fields, where applications can extend to other systems that are facilitated by the modest sample volumes and ease of sample loading and geometry.PostprintPeer reviewe

Wideband corrugated feedhorns, for radar, communications, radiometry and quasi-optics

This work was supported in part by Engineering and Physical Sciences Research Council, U.K., under Grant EP/R013705/1, and in part by IAA Funding.A wide variety of desirable antenna beam patterns can be synthesized by optimal excitation and phasing of the HE11 and HE12 modes in scalar corrugated feedhorns. However, the bandwidth of such two-mode horns is often limited by modal dispersion. In this paper we introduce a class of low dispersion, two-mode feedhorns that can operate, in some cases, over operating bandwidths of 40-50%. We provide example designs that include horns with high coupling efficiency to: 1) a pure HE11 mode for single-mode excitation of corrugated pipe transmission lines; 2) a LG00 and LG02 combination for radiometry, with narrow beams; 3) a pure Laguerre Gaussian LG00 mode for quasi-optical instrumentation with constant phase centers; 4) a constant gain antenna for uniform illumination with frequency; 5) Airy patterns or “top hat” patterns for radar or communications applications, designed to maximize aperture efficiencies when used with larger reflect or lens antennas. More generally, we show methods to generate and phase multiple HE1n modes, to synthesize symmetric output beams at any desired frequency or gain.Publisher PDFPeer reviewe

High-sensitivity Gd3+-Gd3+ EPR distance measurements that eliminate artefacts seen at short distances

We would like to acknowledge EPSRC (EP/R)13705/1) for current funding on the HiPER project, and the Wellcome Trust for a multi-user equipment grant (099149/Z/12/Z) for upgrades on the Q-band system. We thank the Royal Society for an International Exchanges Grant and The Weizmann-UK Joint Research Program for allowing bilateral travel and research between the University of St Andrews and the Weizmann Institute of Science. JEL thanks the Royal Society for a University Research Fellowship. MJT thanks EPSRC for a CM-CDT studentship (EP/LO15110/1). MQ and AG thank the Deutsche Forschungsgemeinschaft (DFG) for funding within SPP 1601 (GO555/6-2).Gadolinium complexes are attracting increasing attention as spin labels for EPR dipolar distance measurements in biomolecules and particularly for in-cell measurements. It has been shown that flip-flop transitions within the central transition of the high spin Gd3+ ion can introduce artefacts in dipolar distance measurements, particularly when measuring distances less than 3–4 nm. Previous work has shown some reduction of these artefacts through increasing the frequency separation between the two frequencies required for the Double Electron-Electron Resonance (DEER) experiment. Here we use a high power (1 kW), wideband, non-resonant, system operating at 94 GHz to evaluate DEER measurement protocols using two rigid Gd(III)-rulers, consisting of two [GdIII(PyMTA)] complexes, with separations of 2.1 nm and 6.0 nm, respectively. We show that by avoiding the |−1/2⟩ → |1/2⟩ central transition completely, and placing both the pump and the observer pulses on either side of the central transition, we can now observe apparently artefact-free spectra and narrow distance distributions, even for a Gd-Gd distance of 2.1 nm. Importantly we still maintain excellent signal-to-noise ratio and relatively high modulation depths. These results have implications for in-cell EPR measurements at naturally occurring biomolecule concentrations.Publisher PDFPeer reviewe

Biohydrogenation of 22:6n-3 by Butyrivibrio proteoclasticus P18

Background: Rumen microbes metabolize 22:6n-3. However, pathways of 22:6n-3 biohydrogenation and ruminal microbes involved in this process are not known. In this study, we examine the ability of the well-known rumen biohydrogenating bacteria, Butyrivibrio fibrisolvens D1 and Butyrivibrio proteoclasticus P18, to hydrogenate 22:6n-3. Results: Butyrivibrio fibrisolvens D1 failed to hydrogenate 22:6n-3 (0.5 to 32 mu g/mL) in growth medium containing autoclaved ruminal fluid that either had or had not been centrifuged. Growth of B. fibrisolvens was delayed at the higher 22:6n-3 concentrations; however, total volatile fatty acid production was not affected. Butyrivibrio proteoclasticus P18 hydrogenated 22:6n-3 in growth medium containing autoclaved ruminal fluid that either had or had not been centrifuged. Biohydrogenation only started when volatile fatty acid production or growth of B. proteoclasticus P18 had been initiated, which might suggest that growth or metabolic activity is a prerequisite for the metabolism of 22:6n-3. The amount of 22:6n-3 hydrogenated was quantitatively recovered in several intermediate products eluting on the gas chromatogram between 22:6n-3 and 22:0. Formation of neither 22:0 nor 22:6 conjugated fatty acids was observed during 22:6n-3 metabolism. Extensive metabolism was observed at lower initial concentrations of 22:6n-3 (5, 10 and 20 mu g/mL) whereas increasing concentrations of 22:6n-3 (40 and 80 mu g/mL) inhibited its metabolism. Stearic acid formation (18:0) from 18:2n-6 by B. proteoclasticus P18 was retarded, but not completely inhibited, in the presence of 22:6n-3 and this effect was dependent on 22:6n-3 concentration. Conclusions: For the first time, our study identified ruminal bacteria with the ability to hydrogenate 22:6n-3. The gradual appearance of intermediates indicates that biohydrogenation of 22:6n-3 by B. proteoclasticus P18 occurs by pathways of isomerization and hydrogenation resulting in a variety of unsaturated 22 carbon fatty acids. During the simultaneous presence of 18:2n-6 and 22:6n-3, B. proteoclasticus P18 initiated 22:6n-3 metabolism before converting 18:1 isomers into 18:0

Why invent the handle? Electromyography (EMG) and efficiency of use data investigating the prehistoric origin and selection of hafted stone knives

Abstract: For over 3 million years hominins held stone-cutting tools in the hand, gripping the portion of tool displaying a sharp cutting edge directly. During the late Middle Pleistocene human populations started to produce hafted composite knives, where the stone element displaying a sharp cutting edge was secured in a handle. Prevailing archaeological literature suggests that handles convey benefits to tool users by increasing cutting performance and reducing musculoskeletal stresses, yet to date these hypotheses remain largely untested. Here, we compare the cutting performance of hafted knives, ‘basic’ flake tools, and large bifacial tools during two standardized cutting tasks. Going further, we examine the comparative ergonomics of each tool type through electromyographic (EMG) analysis of nine upper limb muscles. Results suggest that knives (1) recruit muscles responsible for digit flexion (i.e. gripping) and in-hand manipulation relatively less than alternative stone tool types and (2) may convey functional performance benefits relative to unhafted stone tool alternatives when considered as a generalised cutting tool. Furthermore, our data indicate that knives facilitate greater muscle activity in the upper arm and forearm, potentially resulting in the application of greater cutting forces during tool use. Compared to unhafted prehistoric alternatives, hafted stone knives demonstrate increased ergonomic properties and some functional performance benefits. These factors would likely have contributed to the invention and widespread adoption of hafted stone knives during the late Middle Pleistocene

A phase II evaluation of cediranib in the treatment of recurrent or persistent endometrial cancer: An NRG Oncology/Gynecologic Oncology Group study

PURPOSE: Cediranib is a multi-tyrosine kinase inhibitor targeting vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) receptors. This phase II study was conducted to assess activity and tolerability of single-agent cediranib in recurrent/persistent endometrial cancer. PATIENTS AND METHODS: Eligible patients had recurrent or persistent endometrial cancer after receiving one or two prior cytotoxic regimens, measurable disease, and Gynecologic Oncology Group (GOG) performance status of ≤2 (≤1 if two prior cytotoxic regimens given). Cediranib 30mg orally daily for a 28daycycle was administered until disease progression or prohibitive toxicity. Microvessel density (MVD) was measured in tumor tissue from initial hysterectomy specimens and correlated with clinical outcome. Primary endpoints were tumor response and surviving progression-free for six months without subsequent therapy (6-month event-free survival [EFS]). RESULTS: Of 53 patients enrolled, 48 were evaluable for cediranib efficacy and toxicity. Median age was 65.5 years, 52% of patients had received prior radiation, and 73% of patients received only one prior chemotherapy regimen. A partial response was observed in 12.5%. Fourteen patients (29%) had six-month EFS. Median progression-free survival (PFS) was 3.65 months and median overall survival (OS) 12.5 months. No grade 4 or 5 toxicities were observed. A trend towards improved PFS was found in patients whose tumors expressed high MVD. CONCLUSION: Cediranib as a monotherapy treatment for recurrent or persistent endometrial cancer is well tolerated and met protocol set objectives for sufficient activity to warrant further investigation. MVD may be a useful biomarker for activity