199 research outputs found
A generalized Ramsey excitation scheme with suppressed light shift
We experimentally investigate a recently proposed optical excitation scheme
[V.I. Yudin et al., Phys. Rev. A 82, 011804(R)(2010)] that is a generalization
of Ramsey's method of separated oscillatory fields and consists of a sequence
of three excitation pulses. The pulse sequence is tailored to produce a
resonance signal which is immune to the light shift and other shifts of the
transition frequency that are correlated with the interaction with the probe
field. We investigate the scheme using a single trapped 171Yb+ ion and excite
the highly forbidden 2S1/2-2F7/2 electric-octupole transition under conditions
where the light shift is much larger than the excitation linewidth, which is in
the Hertz range. The experiments demonstrate a suppression of the light shift
by four orders of magnitude and an immunity against its fluctuations.Comment: 5 pages, 4 figure
Atomic clocks with suppressed blackbody radiation shift
We develop a nonstandard concept of atomic clocks where the blackbody
radiation shift (BBRS) and its temperature fluctuations can be dramatically
suppressed (by one to three orders of magnitude) independent of the
environmental temperature. The suppression is based on the fact that in a
system with two accessible clock transitions (with frequencies v1 and v2) which
are exposed to the same thermal environment, there exists a "synthetic"
frequency v_{syn} (v1-e12 v2) largely immune to the BBRS. As an example, it is
shown that in the case of ion 171Yb+ it is possible to create a clock in which
the BBRS can be suppressed to the fractional level of 10^{-18} in a broad
interval near room temperature (300\pm 15 K). We also propose a realization of
our method with the use of an optical frequency comb generator stabilized to
both frequencies v1 and v2. Here the frequency v_{syn} is generated as one of
the components of the comb spectrum and can be used as an atomic standard.Comment: 5 pages, 2 figure
Melt pond fraction and spectral sea ice albedo retrieval from MERIS data – Part 1: Validation against in situ, aerial, and ship cruise data
The presence of melt ponds on the Arctic sea ice strongly affects the energy balance of the Arctic Ocean in summer. It affects albedo as well as transmittance through the sea ice, which has consequences for the heat balance and mass balance of sea ice. An algorithm to retrieve melt pond fraction and sea ice albedo from Medium Resolution Imaging Spectrometer (MERIS) data is validated against aerial, shipborne and in situ campaign data. The results show the best correlation for landfast and multiyear ice of high ice concentrations. For broadband albedo, R2 is equal to 0.85, with the RMS (root mean square) being equal to 0.068; for the melt pond fraction, R2 is equal to 0.36, with the RMS being equal to 0.065. The correlation for lower ice concentrations, subpixel ice floes, blue ice and wet ice is lower due to ice drift and challenging for the retrieval surface conditions. Combining all aerial observations gives a mean albedo RMS of 0.089 and a mean melt pond fraction RMS of 0.22. The in situ melt pond fraction correlation is R2 = 0.52 with an RMS = 0.14. Ship cruise data might be affected by documentation of varying accuracy within the Antarctic Sea Ice Processes and Climate (ASPeCt) protocol, which may contribute to the discrepancy between the satellite value and the observed value: mean R2 = 0.044, mean RMS = 0.16. An additional dynamic spatial cloud filter for MERIS over snow and ice has been developed to assist with the validation on swath data
Genome sequence of the Ornithopus/Lupinus-nodulating Bradyrhizobium sp. strain WSM471
Bradyrhizobium sp. strain WSM471 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-(N-2) fixing root nodule formed on the annual legume Ornithopus pinnatus (Miller) Druce growing at Oyster Harbour, Albany district, Western Australia in 1982. This strain is in commercial production as an inoculant for Lupinus and Ornithopus. Here we describe the features of Bradyrhizobium sp. strain WSM471, together with genome sequence information and annotation. The 7,784,016 bp high-quality-draft genome is arranged in 1 scaffold of 2 contigs, contains 7,372 protein-coding genes and 58 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program
Genome sequence of the Lebeckia ambigua-nodulating 'Burkholderia sprentiae' strain WSM5005T
"Burkholderia sprentiae" strain WSM5005(T) is an aerobic, motile, Gram-negative, non-sporeforming rod that was isolated in Australia from an effective N-2-fixing root nodule of Lebeckia ambigua collected in Klawer, Western Cape of South Africa, in October 2007. Here we describe the features of "Burkholderia sprentiae" strain WSM5005T, together with the genome sequence and its annotation. The 7,761,063 bp high-quality-draft genome is arranged in 8 scaffolds of 236 contigs, contains 7,147 protein-coding genes and 76 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program
IMG/M: the integrated metagenome data management and comparative analysis system
The integrated microbial genomes and metagenomes (IMG/M) system provides support for comparative analysis of microbial community aggregate genomes (metagenomes) in a comprehensive integrated context. IMG/M integrates metagenome data sets with isolate microbial genomes from the IMG system. IMG/M’s data content and analytical capabilities have been extended through regular updates since its first release in 2007. IMG/M is available at http://img.jgi.doe.gov/m. A companion IMG/M systems provide support for annotation and expert review of unpublished metagenomic data sets (IMG/M ER: http://img.jgi.doe.gov/mer)
IMG/M: the integrated metagenome data management and comparative analysis system
The integrated microbial genomes and metagenomes (IMG/M) system provides support for comparative analysis of microbial community aggregate genomes (metagenomes) in a comprehensive integrated context. IMG/M integrates metagenome data sets with isolate microbial genomes from the IMG system. IMG/M's data content and analytical capabilities have been extended through regular updates since its first release in 2007. IMG/M is available at http://img.jgi.doe.gov/m. A companion IMG/M systems provide support for annotation and expert review of unpublished metagenomic data sets (IMG/M ER: http://img.jgi.doe.gov/mer)
Genome sequence of the Trifolium rueppellianum -nodulating Rhizobium leguminosarum bv. trifolii strain WSM2012
Rhizobium leguminosarum bv. trifolii WSM2012 (syn. MAR1468) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an ineffective root nodule recovered from the roots of the annual clover Trifolium rueppellianum Fresen. growing in Ethiopia. WSM2012 has a narrow, specialized host range for N2-fixation. Here we describe the features of R. leguminosarum bv. trifolii strain WSM2012, together with genome sequence information and annotation. The 7,180,565 bp high-quality-draft genome is arranged into 6 scaffolds of 68 contigs, contains 7,080 protein-coding genes and 86 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Progra
Plant microbiomes harbor potential to promote nutrient turnover in impoverished substrates of a Brazilian biodiversity hotspot.
The substrates of the Brazilian campos rupestres, a grassland ecosystem, have extremely low concentrations of phosphorus and nitrogen, imposing restrictions to plant growth. Despite that, this ecosystem harbors almost 15% of the Brazilian plant diversity, raising the question of how plants acquire nutrients in such a harsh environment. Here, we set out to uncover the taxonomic profile, the compositional and functional differences and similarities, and the nutrient turnover potential of microbial communities associated with two plant species of the campos rupestres-dominant family Velloziaceae that grow over distinct substrates (soil and rock). Using amplicon sequencing data, we show that, despite the pronounced composition differentiation, the plant-associated soil and rock communities share a core of highly efficient colonizers that tend to be highly abundant and is enriched in 21 bacterial families. Functional investigation of metagenomes and 522 metagenome-assembled genomes revealed that the microorganisms found associated to plant roots are enriched in genes involved in organic compound intake, and phosphorus and nitrogen turnover. We show that potential for phosphorus transport, mineralization, and solubilization are mostly found within bacterial families of the shared microbiome, such as Xanthobacteraceae and Bryobacteraceae. We also detected the full repertoire of nitrogen cycle-related genes and discovered a lineage of Isosphaeraceae that acquired nitrogen-fixing potential via horizontal gene transfer and might be also involved in nitrification via a metabolic handoff association with Binataceae. We highlight that plant-associated microbial populations in the campos rupestres harbor a genetic repertoire with potential to increase nutrient availability and that the microbiomes of biodiversity hotspots can reveal novel mechanisms of nutrient turnover.Online first
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