1,922 research outputs found
Genome-wide SNP typing of ancient DNA: Determination of hair and eye color of Bronze Age humans from their skeletal remains.
Objective A genome-wide high-throughput single nucleotide polymorphism (SNP) typing method was tested with respect of the applicability to ancient and degraded DNA. The results were compared to mini-sequencing data achieved through single base extension (SBE) typing. The SNPs chosen for the study allow to determine the hair colors and eye colors of humans. Material and methods The DNA samples were extracted from the skeletal remains of 59 human individuals dating back to the Late Bronze Age. The 3,000 years old bones had been discovered in the Lichtenstein Cave in Lower Saxony, Germany. The simultaneous typing of 24 SNPs for each of the ancient DNA samples was carried out using the 192.24 Dynamic Array (TM) by Fluidigm (R). Results Thirty-eight of the ancient samples (=64%) revealed full and reproducible SNP genotypes allowing hair and eye color phenotyping. In 10 samples (=17%) at least half of the SNPs were unambiguously determined, in 11 samples (=19%) the SNP typing failed. For 23 of the 59 individuals, a comparison of the SNP typing results with genotypes from an earlier performed SBE typing approach was possible. The comparison confirmed the full concordance of the results for 90% of the SNP typings. In the remaining 10% allelic dropouts were identified. Discussion The high genotyping success rate could be achieved by introducing modifications to the preamplification protocol mainly by increasing the DNA input and the amplification cycle number. The occurrence of allelic dropouts indicates that a further increase of DNA input to the preamplification step is desirable
Tidally Triggered Star Formation in Close Pairs of Galaxies: Major and Minor Interactions
We study star formation in a sample of 345 galaxies in 167 pairs and compact
groups drawn from the original CfA2 Redshift Survey and from a follow-up search
for companions. We construct our sample with attention to including pairs with
luminosity contrast |\Delta m_R| >= 2. These 57 galaxies with |\Delta m_R| >= 2
provide a set of nearby representative cases of minor interactions, a central
feature of the hierarchical galaxy formation model. Here we report the
redshifts and positions of the 345 galaxies in our sample, and of 136 galaxies
in apparent pairs that are superpositions. In the pairs sample as a whole,
there are strong correlations between the equivalent width of the H\alpha
emission line and the projected spatial and the line-of-sight velocity
separation of the pair. For pairs of small luminosity contrast, |\Delta m_R| <
2, the member galaxies show a correlation between the equivalent width of
H\alpha and the projected spatial separation of the pair. However, for pairs
with large luminosity contrast, |\Delta m_R| >= 2, we detect no correlation
between the equivalent width of H\alpha and the projected spatial separation.
The relative luminosity of the companion galaxy is more important in a
gravitational tidal interaction than the intrinsic luminosity of the galaxy.
Central star formation across the entire pairs sample depends strongly on the
luminosity ratio, |\Delta m_R|, a reasonable proxy for the mass ratio of the
pair; pairs composed of similarly luminous galaxies produce the strongest
bursts of star formation. Pairs with |\Delta m_R| >= 2 rarely have EW(H\alpha)
>~ 70 Ang.Comment: Minor revisions following journal proof
Induced Gamma-band Activity Elicited by Visual Representation of Unattended Objects
Peer reviewedPostprin
The Radio Properties of Composite LINER/HII Galaxies
Arcsec-resolution VLA observations -- newly obtained as well as published --
of 40 nearby galaxies are discussed, completing a study of the radio properties
of a magnitude-limited sample of nearby galaxies of the composite LINER/HII
type. Our results reveal an overall detection rate of at least 25% AGN
candidates among these composite sources. The general properties of these AGN
candidates, as compared to non-AGN composite sources and HII galaxies, are
discussed.Comment: Accepted for publication in ApJ
In B-CLL, the codon 72 polymorphic variants of p53 are not related to drug resistance and disease prognosis
BACKGROUND: A common sequence polymorphism at codon 72 of the p53 gene encoding either arginine or proline was recently shown to be functionally relevant for apoptosis induction in vitro. In B-type chronic lymphocytic leukemia (B-CLL), p53 gene mutations occur in a subset of patients and are associated with impaired survival and drug resistance. Here, we address the functional relevance of the codon 72 single nucleotide (SNP) polymorphism for cell death sensitivity following exposure to clinically employed cytotoxic drugs and gamma-irradiation. METHODS: 138 B-CLL samples were analysed by SSCP-PCR and sequencing for single nucleotide polymorphism at codon 72 of the p53 gene. The in vitro cytotoxicity assay (DiSC-assay) was performed with 7 drugs (chlorambucil, mafosfamide, fludarabine phosphate, methylprednisolone, doxorubicin, vincristine) or gamma-irradiation. RESULTS: Of the 138 B-CLL samples, 9 samples were homozygous for proline (Pro/Pro), 78 samples homozygous for arginine (Arg/Arg), and 49 samples heterozygous (Arg/Pro). No differences were found for patient survival and cell death triggered by 7 cytotoxic drugs or gamma-irradiation. CONCLUSION: These data indicate that polymorphic variants of p53 codon 72 are not clinically relevant for apoptosis induction or patient survival in B-CLL
A Close Look at Star Formation around Active Galactic Nuclei
We analyse star formation in the nuclei of 9 Seyfert galaxies at spatial
resolutions down to 0.085arcsec, corresponding to length scales of less than
10pc in some objects. Our data were taken mostly with the near infrared
adaptive optics integral field spectrograph SINFONI. The stellar light profiles
typically have size scales of a few tens of parsecs. In two cases there is
unambiguous kinematic evidence for stellar disks on these scales. In the
nuclear regions there appear to have been recent - but no longer active -
starbursts in the last 10-300Myr. The stellar luminosity is less than a few
percent of the AGN in the central 10pc, whereas on kiloparsec scales the
luminosities are comparable. The surface stellar luminosity density follows a
similar trend in all the objects, increasing steadily at smaller radii up to
10^{13}L_sun/kpc^2 in the central few parsecs, where the mass surface density
exceeds 10^4M_sun/pc^2. The intense starbursts were probably Eddington limited
and hence inevitably short-lived, implying that the starbursts occur in
multiple short bursts. The data hint at a delay of 50--100Myr between the onset
of star formation and subsequent fuelling of the black hole. We discuss whether
this may be a consequence of the role that stellar ejecta could play in
fuelling the black hole. While a significant mass is ejected by OB winds and
supernovae, their high velocity means that very little of it can be accreted.
On the other hand winds from AGB stars ultimately dominate the total mass loss,
and they can also be accreted very efficiently because of their slow speeds.Comment: 51 pages, including 27 figures; accepted by ApJ (paper reorganised,
but results & conclusions the same
The Nature of Composite LINER/HII Galaxies, As Revealed from High-Resolution VLA Observations
A sample of 37 nearby galaxies displaying composite LINER/HII and pure HII
spectra was observed with the VLA in an investigation of the nature of their
weak radio emission. The resulting radio contour maps overlaid on optical
galaxy images are presented here, together with an extensive literature list
and discussion of the individual galaxies. Radio morphological data permit
assessment of the ``classical AGN'' contribution to the global activity
observed in these ``transition'' LINER galaxies. One in five of the latter
objects display clear AGN characteristics: these occur exclusively in
bulge-dominated hosts.Comment: 31 pages, 27 figures, accepted by ApJ
Anatomy of helical relativistic jets: The case of S5 0836+710
Helical structures are common in extragalactic jets. They are usually
attributed in the literature to periodical phenomena in the source (e.g.,
precession). In this work, we use VLBI data of the radio-jet in the quasar S5
0836+710 and hypothesize that the ridge-line of helical jets like this
corresponds to a pressure maximum in the jet and assume that the helically
twisted pressure maximum is the result of a helical wave pattern. For our
study, we use observations of the jet in S5 0836+710 at different frequencies
and epochs. The results show that the structures observed are physical and not
generated artificially by the observing arrays. Our hypothesis that the
observed intensity ridge-line can correspond to a helically twisted pressure
maximum is confirmed by our observational tests. This interpretation allows us
to explain jet misalignment between parsec and kiloparsec scales when the
viewing angle is small, and also brings us to the conclusion that
high-frequency observations may show only a small region of the jet flow
concentrated around the maximum pressure ridge-line observed at low
frequencies. Our work provides a potential explanation for the apparent
transversal superluminal speeds observed in several extragalactic jets by means
of transversal shift of an apparent core position with time.Comment: Accepted for publication in the Astrophysical Journa
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