Understanding the Interaction Effects between Dietary Lipid Content and Rearing Temperature on Growth Performance, Feed Utilization, and Fat Deposition of Sea Bass (Dicentrarchus labrax)

This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at two different temperature regimes; one starting at 23 °C and then changed to 17 °C for 61 days, and the other starting at 17 °C and then changed to 23 °C for 39 days. Two commercial diets containing both ~44% crude protein but incorporating different dietary lipid levels, 16.5% (D16) and 20.0% (D20) (dry matter (DM)), were fed to the fish to apparent satiation; the type of diet fed to each fish group remained constant throughout the experiment. Final body weight, weight gain, and specific growth rate were significantly higher for the fish group held at 23 °C compared to the fish group at 17 °C (before the temperature changes), while the dietary fat content did not have any profound effect in both groups. Furthermore, the different temperature regimes did not affect muscle or liver composition, but, on the contrary, dietary lipids affected hepatosomatic, perivisceral fat, and visceral indexes. Feed conversion ratio and specific growth rate were not affected by the dietary lipid level. An interaction of temperature and dietary lipid content was observed in daily feed consumption (DFC) and final body weight (FBW).info:eu-repo/semantics/publishedVersio

Assessment of MODIS spectral indices for determining rice paddy agricultural practices and hydroperiod

The aims of this study were to assess the dynamics of the Normalized Difference Vegetation Index (NDVI), Normalized Difference Water Index (NDWI(1) and NDWI(2)) and Shortwave Angle Slope Index (SASI) in relation to rice agricultural practices and hydroperiod, and (2) to assess the capability for these indices to detect phenometrics in rice under different flooding regimes

Understanding the Interaction Effects between Dietary Lipid Content and Rearing Temperature on Growth Performance, Feed Utilization, and Fat Deposition of Sea Bass (<i>Dicentrarchus labrax</i>)

This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at two different temperature regimes; one starting at 23 °C and then changed to 17 °C for 61 days, and the other starting at 17 °C and then changed to 23 °C for 39 days. Two commercial diets containing both ~44% crude protein but incorporating different dietary lipid levels, 16.5% (D16) and 20.0% (D20) (dry matter (DM)), were fed to the fish to apparent satiation; the type of diet fed to each fish group remained constant throughout the experiment. Final body weight, weight gain, and specific growth rate were significantly higher for the fish group held at 23 °C compared to the fish group at 17 °C (before the temperature changes), while the dietary fat content did not have any profound effect in both groups. Furthermore, the different temperature regimes did not affect muscle or liver composition, but, on the contrary, dietary lipids affected hepatosomatic, perivisceral fat, and visceral indexes. Feed conversion ratio and specific growth rate were not affected by the dietary lipid level. An interaction of temperature and dietary lipid content was observed in daily feed consumption (DFC) and final body weight (FBW)

Understanding the Interaction Effects between Dietary Lipid Content and Rearing Temperature on Growth Performance, Feed Utilization, and Fat Deposition of Sea Bass (Dicentrarchus labrax)

This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at two different temperature regimes; one starting at 23 °C and then changed to 17 °C for 61 days, and the other starting at 17 °C and then changed to 23 °C for 39 days. Two commercial diets containing both ~44% crude protein but incorporating different dietary lipid levels, 16.5% (D16) and 20.0% (D20) (dry matter (DM)), were fed to the fish to apparent satiation; the type of diet fed to each fish group remained constant throughout the experiment. Final body weight, weight gain, and specific growth rate were significantly higher for the fish group held at 23 °C compared to the fish group at 17 °C (before the temperature changes), while the dietary fat content did not have any profound effect in both groups. Furthermore, the different temperature regimes did not affect muscle or liver composition, but, on the contrary, dietary lipids affected hepatosomatic, perivisceral fat, and visceral indexes. Feed conversion ratio and specific growth rate were not affected by the dietary lipid level. An interaction of temperature and dietary lipid content was observed in daily feed consumption (DFC) and final body weight (FBW)

O86 | Combination of antibiotic and bacteriophages to prevent the selection of resistant <i>Pseudomonas aeruginosa</i>

International audienceIntroduction: Pseudomonas aeruginosa is an opportunistic pathogen, naturally resistant to many antibiotics. The development of additional drug resistance after repeated antibiotic treatments can lead to therapeutic gaps. Among alternative therapeutic strategies to antibiotics, bacteriophages (phages), antibacterial viruses that have the particular ability to self-amplify at the site of infection, were proposed. Nevertheless, as with antibiotics, bacteria have developed several ways to resist phages. However, as the molecular mechanisms involved in drug and phage resistance do not overlap, their combination in cocktails or with antibiotics has been proposed to improve treatment efficacy. Here, we tested the combination of ciprofloxacin and phages on P. aeruginosa by mimicking the pharmacokinetic profiles of ciprofloxacin in patients over several days.Methods: Using a hollow fiber infection model (HFIM) inoculated with P. aeruginosa, we simulated the pharmacokinetics of ciprofloxacin in the lungs during oral treatment of patients for 72 h. A single local inhalation treatment with phages was mimicked with one phage or a cocktail of two phages directly inoculated in the extracapillary space of the HFMI cartridge. Ciprofloxacin and phages alone and in combination were tested on a standard bacterial inoculum of 5.5 log10 CFU/mL and a high inoculum of 8.5 log10 CFU/mL. For the combinations, the first dose of ciprofloxacin was injected into the HFMI at the same time as the phages or 4 h after them.Results: Ciprofloxacin alone, a single phage, or a cocktail of two phages were unable to inhibit the growth of antibiotic- or phage-resistant P. aeruginosa within 30 h. With the standard inoculum, the combination of ciprofloxacin and phages eradicated bacteria regardless of the timing of ciprofloxacin administration. For the high inoculum, bacterial regrowth and resistance selection were prevented by the combination when the first dose of ciprofloxacin was added in the HFIM 4 h after phage inoculation.Conclusion: Our data, demonstrating the eradication of bacteria and the prevention of resistance by the combination of ciprofloxacin and phages, call for in vivo experiments to probe their clinical translation. These results constitute a proof-of-concept for innovative therapeutic strategies against Gram-negative pathogens in veterinary medicine, combining phages and veterinary antimicrobials

The Selection of Antibiotic- and Bacteriophage-Resistant Pseudomonas aeruginosa Is Prevented by Their Combination

International audienceBacteria developing resistance compromise the efficacy of antibiotics or bacteriophages (phages). We tested the association of these two antibacterials to circumvent resistance. With the Hollow Fiber Infection Model (HFIM), we mimicked the concentration profile of ciprofloxacin in the lungs of patients treated orally for Pseudomonas aeruginosa infections and, independently, mimicked a single inhaled administration of phages (one or two phages). Each treatment selects for antibiotic- or phage-resistant clones in less than 30 h. In contrast, no bacteria were recovered from the HFIM at 72 h when ciprofloxacin was started 4 h post phage administration, even when increasing the initial bacterial concentration by 1,000-fold. The combination of phages with antibiotics used according to clinical regimens prevents the growth of resistant clones, providing opportunities to downscale the use of multiple antibiotics. IMPORTANCE In the treatment of bacterial infections, the use of antibiotics or bacteriophages (phages) is limited by the ability of bacteria to develop resistance. The resistance frequency depends on the exposure to antibacterials. Therefore, determination of concentration profiles of antibiotics is key to define optimal regimens during treatments. In the laboratory, the Hollow Fiber Infection Model (HFIM) mimics concentration profiles observed in patients. In this study, we used the HFIM to evaluate the killing efficacy of the combination of phages and ciprofloxacin. We demonstrated that dosing schedule of phages first and the antibiotic second prevent the selection of resistant bacteria. These results demonstrate that combination efficacy relies on a strong initial reduction of the bacterial population by phages followed by antibiotics before any resistant arise

Mapping Periodic Patterns of Global Vegetation Based on Spectral Analysis of NDVI Time Series

Vegetation seasonality assessment through remote sensing data is crucial to understand ecosystem responses to climatic variations and human activities at large-scales. Whereas the study of the timing of phenological events showed significant advances, their recurrence patterns at different periodicities has not been widely study, especially at global scale. In this work, we describe vegetation oscillations by a novel quantitative approach based on the spectral analysis of Normalized Difference Vegetation Index (NDVI) time series. A new set of global periodicity indicators permitted to identify different seasonal patterns regarding the intra-annual cycles (the number, amplitude, and stability) and to evaluate the existence of pluri-annual cycles, even in those regions with noisy or low NDVI. Most of vegetated land surface (93.18%) showed one intra-annual cycle whereas double and triple cycles were found in 5.58% of the land surface, mainly in tropical and arid regions along with agricultural areas. In only 1.24% of the pixels, the seasonality was not statistically significant. The highest values of amplitude and stability were found at high latitudes in the northern hemisphere whereas lowest values corresponded to tropical and arid regions, with the latter showing more pluri-annual cycles. The indicator maps compiled in this work provide highly relevant and practical information to advance in assessing global vegetation dynamics in the context of global change

Ecosystem functional assessment based on the "optical type" concept and self-similarity patterns: An application using MODIS-NDVI time series autocorrelation

Remote sensing (RS) time series are an excellent operative source for information about the land surface across several scales and different levels of landscape heterogeneity. Ustin and Gamon (2010) proposed the new concept of "optical types" (OT), meaning "optically distinguishable functional types", as a way to better understand remote sensing signals related to the actual functional behavior of species that share common physiognomic forms but differ in functionality. Whereas the OT approach seems to be promising and consistent with ecological theory as a way to monitor vegetation derived from RS, it received little implementation. This work presents a method for implementing the OT concept for efficient monitoring of ecosystems based on RS time series. We propose relying on an ecosystem's repetitive pattern in the temporal domain (self-similarity) to assess its dynamics. Based on this approach, our main hypothesis is that distinct dynamics are intrinsic to a specific OT. Self-similarity level in the temporal domain within a broadleaf forest class was quantitatively assessed using the auto-correlation function (ACF), from statistical time series analysis. A vector comparison classification method, spectral angle mapper, and principal component analysis were used to identify general patterns related to forest dynamics. Phenological metrics derived from MOD IS NDVI time series using the TIMESAT software, together with information from the National Forest Map were used to explain the different dynamics found. Results showed significant and highly stable self-similarity patterns in OTs that corresponded to forests under non-moisture-limited environments with an adaptation strategy based on a strong phenological synchrony with climate seasonality. These forests are characterized by dense closed canopy deciduous forests associated with high productivity and low biodiversity in terms of dominant species. Forests in transitional areas were associated with patterns of less temporal stability probably due to mixtures of different adaptation strategies (i.e., deciduous, marcescent and evergreen species) and higher functional diversity related to climate variability at long and short terms. A less distinct seasonality and even a double season appear in the OT of the broadleaf Mediterranean forest characterized by an open canopy dominated by evergreen-sclerophyllous formations. Within this forest, understory and overstory dynamics maximize functional diversity resulting in contrasting traits adapted to summer drought, winter frosts, and high precipitation variability. (C) 2015 Elsevier B.V. All rights reserved

Impact of the polycarbonate strippers used in assisted reproduction techniques on embryonic development

International audienceStudy question: Do daily manipulations of preimplantation embryos with polycarbonate (PC)-made bisphenol A (BPA)-releasing strippers influence embryo development?Summary answer: Compared to glass strippers, PC strippers enhance the blastocyst development rate but this does not seem to be BPA-related.What is known already: PC strippers have been shown to release tiny amounts (around 0.5 ng/ml BPA) of BPA in routine human IVF procedures. A chronic exposure to BPA either in vivo or in vitro during the preimplantation period can impact post-implantation and post-natal development. BPA can act rapidly by binding to membrane receptors and inducing rapid non-genomic effects.Study design, size, duration: This experimental study using mouse embryos had a balanced design and blinded evaluations of the endpoints.Participants/materials, setting, methods: In vivo fertilized zygotes were obtained from outbred Swiss CD1 mice crossings after an ovarian stimulation. The zygotes were allocated to three daily handling conditions (HCs) and cultured until Day 4 in a single human commercial medium. Each day, the embryos were handled for 20 s either in a PC stripper (HC1) or in a glass stripper (HC2). In HC3, the embryos were pre-exposed to 0.5 ng/ml BPA before being handled for 20 s in a glass stripper. Handling operations were repeated on Days 1, 2 and 3. Embryo development was assessed blindly on Day 4. Expanded blastocysts were selected for a transcriptomic analysis using Agilent Sureprint G3 Mouse GE v2 microarrays and the retrotransposon LINE1-Orf2 expression was analysed using qRT-PCR, as a proxy for a global evaluation of the epigenetic status.Main results and the role of chance: Compared to the embryos manipulated in HC2 (n = 243), those in HC1 (n = 228) developed significantly more often to the blastocyst stage (55 vs 46%; P < 0.05). It appears the effect of these PC strippers was not BPA-related because embryos pre-exposed to BPA (HC3, n = 230) showed no difference in the blastocyst rate when compared to HC2 (43 vs 46%). When analysing same-stage blastocysts, we noticed no difference in the embryo gene expression between the three HC groups.Large scale data: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE148868.Limitations, reasons for caution: Our results using a mouse model designed to mimic human conditions (outbred strain, human commercial IVF dishes and a unique commercial human embryonic culture media) are reassuring since no gene was found to be differentially expressed, including LINE-1 genes, as a proxy for a global evaluation of the epigenetic status. However, no global epigenetic analysis of the genome has been performed. Furthermore, we did not evaluate post-implantation events, although BPA exposure during peri-conception could affect foeto-placental and post-natal development.Wider implications of the findings: Based on the precautionary principle, several European countries banned the use of BPA in baby bottles and food packaging several years before European Agencies took an official position. The question of applying this principle to plastics in closed contact with human embryos is raised. Further studies are needed for a decision to be made