1,440 research outputs found

    Enforcing Wildfire Prevention Regulations: Evaluating Enforcement Agencies in the County of San Luis Obispo

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    This project aims to evaluate the enforcement of wildfire prevention policies in the County of San Luis Obispo. In the past few years, a series of highly destructive wildfires have ravaged the state of California, leading to scrutiny of wildfire prevention and its enforcement. This study focused on the County of San Luis Obispo to analyze the agencies responsible for enforcing wildfire prevention. In the County of San Luis Obispo, the County Planning and Building Department, County Fire Department, and Cal Fire State Department cooperate to enforce wildfire prevention measures across the County. Personnel from these agencies were interviewed to provide a basis for understanding each agency’s modus operandi. These agencies cooperate to enforce a plethora of preventative regulations, as well as self-perform preventative measures across the County. Enforcement agencies face a multitude of difficulties in enforcing wildfire prevention regulations, including lack of funding, insufficient manpower, and critical media attention. Despite these difficulties, the County of San Luis Obispo operates at a reasonable level considering their resources; however, greater measures could be taken should changes occur. Some of these changes could include greater values for fines, stricter treatment of code violators, and distribution of some County responsibilities to city agencies

    Habitat Utilization by Bottlenose Dolphins (Tursiops truncatus) in Biscayne Bay, Florida

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    Bottlenose dolphins (Tursiops truncatus) exhibit variable distribution patterns, depending upon their geographic location. Habitat utilization patterns in Biscayne Bay, Florida, were examined using the Biscayne Bay Bottlenose Dolphin Photo-ID database obtained from the National Oceanic and Atmospheric Administration (NOAA) Southeast Fisheries Science Center (SEFSC) Miami Laboratory. Habitat coverages in Biscayne Bay were obtained from the Atlas of Marine Resources Version 1.3B CD and the Biscayne Bay National Park CD. Dolphin sightings were overlaid on the habitat coverages using GIS Arcview software. The effects of habitat, season, behavior, zone (sectioned area of Biscayne Bay), and depth on patterns of bottlenose dolphin distribution were examined by analysis of variance to determine the significance of the factors. The total number of dolphins observed during the sightings analyzed was 1,538. The number of dolphins per sighting varied from 1 to 28 dolphins, with a mean of 5.14. The average number of dolphins per survey effort was 10.32. Several significant changes in habitat have occurred between 1991-1992 to 1997. The changes in habitat had some influence on the dolphins’ behavior distribution. The highest proportion of all behavior types was found in moderately dense seagrass beds and dredge bottom areas. Habitat quality (habitat types) of Biscayne Bay influenced dolphin sightings, while habitat quantity (habitat area) influenced dolphin numbers. Analysis of variance statistics supported the strong significant effect of habitat on the variation of sightings and dolphin numbers (P \u3c 0.001). No significant difference in sightings was found between seasons or zones throughout the study period. The fall season had the lowest number of dolphins and sightings. The low number of surveys during the fall season does not account for all the influence on the dolphin numbers. Strong significant differences were observed between behaviors (P \u3c 0.001). The majority of initial behaviors included traveling, feeding, and socializing. Changes in behaviors were apparent as observations continued. It was determined that the proximity of the research vessel and the duration of observation influenced dolphin behavior. Tail slap and chuffing behavior and boat interaction doubled and quadrupled, respectively during sightings. A strong variation in the number of sightings and number of dolphins occurred between different depths (P \u3c 0.001). The majority of dolphins were observed in depths of 2.1 - 3 meters. This coincides with the fact that the majority of Biscayne Bay depths are within that range. A time series analysis was performed to determine if there was a cycle present in the pattern of dolphin distribution, and no significant cycle was found. Future analysis of dolphin composition (resident, migratory, nomadic) may yield cyclic patterns

    A novel interaction of pokeweed antiviral protein with translation initiation factors 4G and iso4G: a potential indirect mechanism to access viral RNAs

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    Pokeweed antiviral protein (PAP) is a ribosome inactivating protein recognized primarily for its ability to depurinate the sarcin/ricin loop of the large rRNA. Studies have demonstrated that PAP also depurinates other RNA templates, such as Human immunodeficiency virus-1 RNA and Brome mosaic virus RNAs. However, the mechanism by which PAP accesses viral RNAs is not known. Considering that PAP was shown recently to bind the m(7)G of the cap structure, we speculated that PAP may interact with other factors involved in translation initiation. By far western analysis, we show that PAP binds specifically to eIF4G and eIFiso4G of wheat germ and analysis with truncation mutants of eIFiso4G indicates that a region of this protein, between amino acids 511 and 624, is required for PAP binding activity. The yeast two-hybrid system supports these results by showing reduced growth and α-galactosidase expression with truncation in this region of eIFiso4G. PAP binds m(7)GTP-Sepharose and this interaction does not diminish the binding of PAP to purified eIFiso4G, indicating that a complex can form among the cap structure, PAP and eIFiso4G. We incubated PAP with uncapped and non-polyadenylated transcripts containing a 3′ translation enhancer sequence (TE) known to increase translation of the RNA in an eIF4F dependent manner. We show that in the presence of wheat germ lysate, PAP depurinates the uncapped and non-polyadenylated transcripts containing a functional wild-type 3′TE, but does not depurinate messages containing a non-functional mutant 3′TE. These results support our hypothesis that binding of PAP to eIF4G and eIFiso4G can provide a mechanism for PAP to access both uncapped and capped viral RNAs for depurination

    Anti-phosphocholine hybridoma antibodies. I. Direct evidence for three distinct families of antibodies in the murine response

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    Biochemical and serological studies were performed on more than 400 anti- phosphocholine (PC) hybridoma proteins (HP) derived from six strains of mice; 26 of these HP were examined in detail. All HP possessed specificity for PC, and all those tested contained an H-chain idiotypic determinant, V(H)-PC, which is shared by PC-binding myeloma proteins (BMP) and anti-PC antibodies. Among the HP, three well-defined and distinct families that correlated well with previous studies on serum anti-PC antibodies were identified. The largest group shared idotypic determinants, an L-chain isoelectric focusing (IEF) pattern, and a binding site specificity with the PC-BMP, T15. Using the same criteria, a second group was found to be strikingly similar to another PC-BMP, M603. The third group possessed an idiotypic determinant and an L-chain IEF profile similar to M511, but differences in binding site specificities were observed among the HP. The latter two groups contained members whose L-chain IEF profiles were not identical to other members of that group. Thus, among strains there is a remarkable degree of conservation among responding anti-PC antibodies, in both the kinds of anti-PC families that exist and the immunochemical and structural characteristics of various members within a family. Differences in at least one parameter were observed in each family, demonstrating that even a relatively restricted response is heterogeneous. However, this diversity seems to operate within certain constraints

    Structured reactive programming with polymorphic temporal tiles

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    International audienceIn functional reactive programming (FRP), system inputs and outputs are generally modeled as functions over continuous time (behaviors) whose future values are governed by sudden changes (events). In this approach, discrete events are embedded into piece-wise continuous behaviors. In the field of reactive music system programming, we develop an orthogonal approach that seems to better fit our need. Much like piano keys can be played and combined both in sequence and in parallel, we model system inputs and outputs as spatio-temporal combinations of what we call temporal values: continuous functions over time whose domain lays between two events: a start and a stop event. Various high level data types and program constructs can then be derived from such a model. They are shown to satisfy robust algebraic and category theoretic properties. Altogether, this eventually provides a simple, robust and elegant programming front-end, temporal tile programming, for reading, memorizing, stretching, combining and transforming flows of inputs into flows of outputs. Although at its infancy, the resulting approach has been experimentally validated for reactive and real-time music system programming

    A comparison of single-cycle versus multiple-cycle proof testing strategies

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    An evaluation of single-cycle and multiple-cycle proof testing (MCPT) strategies for SSME components is described. Data for initial sizes and shapes of actual SSME hardware defects are analyzed statistically. Closed-form estimates of the J-integral for surface flaws are derived with a modified reference stress method. The results of load- and displacement-controlled stable crack growth tests on thin IN-718 plates with deep surface flaws are summarized. A J-resistance curve for the surface-cracked configuration is developed and compared with data from thick compact tension specimens. The potential for further crack growth during large unload/reload cycles is discussed, highlighting conflicting data in the literature. A simple model for ductile crack growth during MCPT based on the J-resistance curve is used to study the potential effects of key variables. The projected changes in the crack size distribution during MCPT depend on the interactions between several key parameters, including the number of proof cycles, the nature of the resistance curve, the initial crack size distribution, the component boundary conditions (load vs. displacement control), and the magnitude of the applied load or displacement. The relative advantages of single-cycle and multiple-cycle proof testing appear to be specific, therefore, to individual component geometry, material, and loading

    Linking the Conservation of Culture and Nature: A Case Study of Sacred Forests in Zimbabwe

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    This paper examines the role of traditional religious beliefs and traditional leaders in conserving remnant patches of a unique type of dry forest in the Zambezi Valley of northern Zimbabwe. We examined aerial photographs spanning more than three decades, interviewed and surveyed local residents, and met with communities to learn about the environmental history of the forests and the factors that have affected land use in the area. Our results show that forest loss is dramatically less in forests that are now considered sacred, or were in the past connected to sacred forests. This supports our hypothesis that traditional spiritual values have influenced human behavior affecting the forests, and have played a role in protecting them until now. We also found that rates of forest loss have been much higher in an area where traditional leaders are relatively disempowered within the post-independence political system compared to an area where traditional leaders have more power. These findings lead us to conclude that a strategy that links the conservation of culture and nature is likely to be more effective in conserving forests than a strategy that ignores traditional beliefs, values, and institutions
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