Characterization of humoral immune responses and degree of protection induced by influenza vaccine in cotton rats:Effects of low vaccine dose and single vs booster vaccination

Introduction: Cotton rats are a suitable model for the study of influenza disease symptoms and responses to influenza vaccination. We have previously shown that two immunizations with 15 µg whole inactivated virus (WIV) influenza vaccine could completely protect animals from infection with the H1N1pdm09 virus. Methods: To further explore the cotton rat model, we here investigated the protective potential of a single intramuscular immunization and of prime/boost intramuscular immunizations with a low amount of antigen. Results: A single intramuscular immunization with doses more than or equal to 0.5 µg WIV reliably evoked antibody responses and doses more than or equal to 1 µg protected the animals from virus replication in the lungs and from severe weight loss. However, clinical symptoms like an increased respiration rate were still apparent. Administration of a booster dose significantly increased the humoral immune responses but did not or only moderately improved protection from clinical symptoms. Conclusion: Our data suggest that complete and partial protection by influenza vaccines can be mimicked in cotton rats by using specific vaccination regimens

Closing the gap: understanding the impact of institutional financial support on student success

This report discusses the development of a statistical model for the evaluation of the effectiveness of institution's financial support packages (bursaries, scholarships and discounts). The model can: track recipients from enrolment to graduate outcomes through student records data compare outcomes of bursary recipients with those slightly and significantly better off (by household income) evaluate the performance of recipients over time and in relation to changes in bursary support levels/conditions be used collaboratively for institutional comparative purposes link (via student records data) with the survey instrument developed as part of this projec

Testing a 1-D analytical salt intrusion model and its predictive equations in Malaysian estuaries

Little is known about the salt intrusion behaviour in Malaysian estuaries. Study of salt intrusion generally requires large amounts of data, especially if 2-D or 3-D numerical models are used; thus, in data-poor environments, 1-D analytical models are more appropriate. A fully analytical 1-D salt intrusion model, which is simple to implement and requires minimal data, was tested in six previously unsurveyed Malaysian estuaries (Kurau, Perak, Bernam, Selangor, Muar and Endau). The required data can be collected during a single day of observations. Site measurements were conducted during the dry season (June–August 2012 and February–March 2013) near spring tide. Data on cross-sections (by echo-sounding), water levels (by pressure loggers) and salinity (by moving boat) were collected as model input. A good fit was demonstrated between the simulated and observed salinity distribution for all six estuaries. Additionally, the two calibration parameters (the Van der Burgh coefficient and the boundary condition for the dispersion) were compared with the existing predictive equations. Since gauging stations were only present in some nested catchments in the drainage basins, the river discharge had to be up-scaled to represent the total discharge contribution of the catchments. However, the correspondence between the calibration coefficients and the predictive equations was good, particularly in view of the uncertainty in the river discharge data used. This confirms that the predictive salt intrusion model is valid for the cases studied in Malaysia. The model provides a reliable, predictive tool, which the water authority of Malaysia can use for making decisions on water abstraction or dredging

Histamine can be Formed and Degraded in the Human and Mouse Heart

Histamine is metabolized by several enzymes in vitro and in vivo. The relevance of this metabolism in the mammalian heart in vivo is unclear. However, histamine can exert positive inotropic effects (PIE) and positive chronotropic effects (PCE) in humans via H2- histamine receptors. In transgenic mice (H2-TG) that overexpress the human H2 receptor in cardiomyocytes but not in wild-type littermate mice (WT), histamine induced PIE and PCE in isolated left or right atrial preparations. These H2-TG were used to investigate the putative relevance of histamine degrading enzymes in the mammalian heart. Histidine, the precursor of histamine, increased force of contraction (FOC) in human atrial preparations. Moreover, histamine increased the phosphorylation state of phospholamban in human atrium. Here, we could detect histidine decarboxylase (HDC) and histamine itself in cardiomyocytes of mouse hearts. Moreover, our data indicate that histamine is subject to degradation in the mammalian heart. Inhibition of the histamine metabolizing enzymes diamine oxidase (DAO) and monoamine oxidase (MAO) shifted the concentration response curves for the PIE in H2-TG atria to the left. Moreover, activity of histamine metabolizing enzymes was present in mouse cardiac samples as well as in human atrial samples. Thus, drugs used for other indication (e.g. antidepressants) can alter histamine levels in the heart. Our results deepen our understanding of the physiological role of histamine in the mouse and human heart. Our findings might be clinically relevant because we show enzyme targets for drugs to modify the beating rate and force of the human heart

The End or Beginning? Either Way, the Credits Are Not Rolling Yet!

(First paragraph) Thank you to all our reviewers, editorial board members, authors, and those who chose the Journal of Urban Mathematics Education (JUME) as their outlet of choice this past year. JUME has had many recent successes, and we in the editorial team plan to release the salient performance data for the journal. For JUME to advance its mission, we believe that accountability and transparency are essential. To this end, our readers will from now on receive an annual progress report about JUME in our first issue of each year

Interlaboratory performance of a Real-Time PCR method for detection of Ceratocystis platani, the agent of canker stain of Platanus spp

Ceratocystis platani (CP), an ascomycetous fungus, is the agent of canker stain, a lethal vascular disease of Platanus species. Ceratocystis platani has been listed as a quarantine pest (EPPO A2 list) due to extensive damage caused in Southern Europe and the Mediterranean region. As traditional diagnostic assays are ineffective, a Real-Time PCR detection method based on EvaGreen, SYBR Green, and Taqman assays was previously developed, validated in-house, and included in the official EPPO standard PM7/14 (2). Here, we describe the results of a test performance study performed by nine European laboratories for the purpose of an interlaboratory validation. Verification of the DNA extracted from biological samples guaranteed the high quality of preparations, and the stability and the homogeneity of the aliquots intended for the laboratories. All of the laboratories reproduced nearly identical standard curves with efficiencies close to 100%. Testing of blind-coded DNA extracted from wood samples revealed that all performance parameters-diagnostic sensitivity, diagnostic specificity, accuracy and reproducibility-were best fit in most cases both at the laboratory and at the assay level. The previously established limit of detection, 3 fg per PCR reaction, was also validated with similar excellent results. The high interlaboratory performance of this Real-Time PCR method confirms its value as a primary tool to safeguard C. platani-free countries by way of an accurate monitoring, and to investigate the resistance level of potentially canker stain-resistant Platanus genotypes

Tutorials at PPSN 2016

PPSN 2016 hosts a total number of 16 tutorials covering a broad range of current research in evolutionary computation. The tutorials range from introductory to advanced and specialized but can all be attended without prior requirements. All PPSN attendees are cordially invited to take this opportunity to learn about ongoing research activities in our field