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FGF-4 signaling is involved in mir-206 expression in developing somites of chicken embryos

The microRNAs (miRNAs) are recently discovered short, noncoding RNAs, that regulate gene expression in metazoans. We have cloned short RNAs from chicken embryos and identified five new chicken miRNA genes. Genome analysis identified 17 new chicken miRNA genes based on sequence homology to previously characterized mouse miRNAs. Developmental Northern blots of chick embryos showed increased accumulation of most miRNAs analyzed from 1.5 days to 5 days except, the stem cell-specific mir-302, which was expressed at high levels at early stages and then declined. In situ analysis of mature miRNAs revealed the restricted expression of mir-124 in the central nervous system and of mir-206 in developing somites, in particular the developing myotome. In addition, we investigated how miR-206 expression is controlled during somite development using bead implants. These experiments demonstrate that fibroblast growth factor (FGF) -mediated signaling negatively regulates the initiation of mir-206 gene expression. This may be mediated through the effects of FGF on somite differentiation. These data provide the first demonstration that developmental signaling pathways affect miRNA expression. Thus far, miRNAs have not been studied extensively in chicken embryos, and our results show that this system can complement other model organisms to investigate the regulation of many other miRNAs

Integrative Sustainability Analysis of European Pig Farms: Development of a Multi-Criteria Assessment Tool

Societal interest in all aspects of sustainability has increased. Therefore, pig farmers need to be aware of their strengths and weaknesses in all dimensions of sustainability: economy, environment, social wellbeing, and animal health and welfare. Our aim was to describe and critically discuss the development of a sustainability assessment tool for pig farms and to evaluate its suitability by applying it to 63 European pig farms (13 breeding, 27 breeding-to-finishing, and 23 finishing farms). The multi-criteria assessment tool was developed in several steps (the selection and scaling of indicators and their aggregation and weighting) in order to summarise the indicators into subtheme and theme scores. The indicators contributing the most to the subtheme/theme scores were identified and discussed in order to evaluate the procedure of the development. For example, some indicators, such as Ecological compensation area, Fairness of prices, and Tail docking, for which farms were scored low, were also identified as “real world problems” in other studies. For other sustainability aspects with low performance, the threshold might have been set too ambitiously, e.g., for Number of sows per annual working unit. Furthermore, to analyse the suitability of the tool, we assessed the best and worst median theme scores (good and poor performances) for each dimension, as well as the variability of the performances of the farms within the themes. Some themes were found to be moderate, such as Pig comfort, Biodiversity, or Resilience, whereas others were found to be good, e.g., Water and the Human–animal relationship, as well as several themes of the social wellbeing dimension. Overall, the sustainability tool provides a comprehensive assessment of the sustainability of pig production. Furthermore, this publication contributes to both the theory (development of a robust sustainability tool) and the practice (provision of a tool to assess and benchmark the sustainability on farms). As a next step, a sensitivity analysis should be performed, and the tool should be applied for further development

Integrative Sustainability Analysis of European Pig Farms: Development of a Multi-Criteria Assessment Tool

Societal interest in all aspects of sustainability has increased. Therefore, pig farmers need to be aware of their strengths and weaknesses in all dimensions of sustainability: economy, environment, social wellbeing, and animal health and welfare. Our aim was to describe and critically discuss the development of a sustainability assessment tool for pig farms and to evaluate its suitability by applying it to 63 European pig farms (13 breeding, 27 breeding-to-finishing, and 23 finishing farms). The multi-criteria assessment tool was developed in several steps (the selection and scaling of indicators and their aggregation and weighting) in order to summarise the indicators into subtheme and theme scores. The indicators contributing the most to the subtheme/theme scores were identified and discussed in order to evaluate the procedure of the development. For example, some indicators, such as Ecological compensation area, Fairness of prices, and Tail docking, for which farms were scored low, were also identified as “real world problems” in other studies. For other sustainability aspects with low performance, the threshold might have been set too ambitiously, e.g., for Number of sows per annual working unit. Furthermore, to analyse the suitability of the tool, we assessed the best and worst median theme scores (good and poor performances) for each dimension, as well as the variability of the performances of the farms within the themes. Some themes were found to be moderate, such as Pig comfort, Biodiversity, or Resilience, whereas others were found to be good, e.g., Water and the Human–animal relationship, as well as several themes of the social wellbeing dimension. Overall, the sustainability tool provides a comprehensive assessment of the sustainability of pig production. Furthermore, this publication contributes to both the theory (development of a robust sustainability tool) and the practice (provision of a tool to assess and benchmark the sustainability on farms). As a next step, a sensitivity analysis should be performed, and the tool should be applied for further development

Blood lactose after dairy product intake in healthy men.

The absence of a dedicated transport for disaccharides in the intestine implicates that the metabolic use of dietary lactose relies on its prior hydrolysis at the intestinal brush border. Consequently, lactose in blood or urine has mostly been associated with specific cases in which the gastrointestinal barrier is damaged. On the other hand, lactose appears in the blood of lactating women and has been detected in the blood and urine of healthy men, indicating that the presence of lactose in the circulation of healthy subjects is not incompatible with normal physiology. In this cross-over study we have characterised the postprandial kinetics of lactose, and its major constituent, galactose, in the serum of fourteen healthy men who consumed a unique dose of 800 g milk or yogurt. Genetic testing for lactase persistence and microbiota profiling of the subjects were also performed. Data revealed that lactose does appear in serum after dairy intake, although with delayed kinetics compared with galactose. Median serum concentrations of approximately 0·02 mmol/l lactose and approximately 0·2 mmol/l galactose were observed after the ingestion of milk and yogurt respectively. The serum concentrations of lactose were inversely correlated with the concentrations of galactose, and the variability observed between the subjects' responses could not be explained by the presence of the lactase persistence allele. Finally, lactose levels have been associated with the abundance of the Veillonella genus in faecal microbiota. The measurement of systemic lactose following dietary intake could provide information about lactose metabolism and nutrient transport processes under normal or pathological conditions

The effect of steps to promote higher levels of farm animal welfare across the EU. Societal versus animal scientists’ perceptions of animal welfare

Information about animal welfare standards and initiatives from eight European countries was collected, grouped, and compared to EU welfare standards to detect those aspects beyond minimum welfare levels demanded by EU welfare legislation. Literature was reviewed to determine the scientific relevance of standards and initiatives, and those aspects going beyond minimum EU standards. Standards and initiatives were assessed to determine their strengths and weaknesses regarding animal welfare. Attitudes of stakeholders in the improvement of animal welfare were determined through a Policy Delphi exercise. Social perception of animal welfare, economic implications of upraising welfare levels, and differences between countries were considered. Literature review revealed that on-farm space allowance, climate control, and environmental enrichment are relevant for all animal categories. Experts’ assessment revealed that on-farm prevention of thermal stress, air quality, and races and passageways’ design were not sufficiently included. Stakeholders considered that housing conditions are particularly relevant regarding animal welfare, and that animal-based and farm-level indicators are fundamental to monitor the progress of animal welfare. The most notable differences between what society offers and what farm animals are likely to need are related to transportation and space availability, with economic constraints being the most plausible explanation

Evaluation of the Antimicrobial Activity of Cationic Polymers against Mycobacteria: Toward Antitubercular Macromolecules.

Antimicrobial resistance is a global healthcare problem with a dwindling arsenal of usable drugs. Tuberculosis, caused by Mycobacterium tuberculosis, requires long-term combination therapy and multi- and totally drug resistant strains have emerged. This study reports the antibacterial activity of cationic polymers against mycobacteria, which are distinguished from other Gram-positive bacteria by their unique cell wall comprising a covalently linked mycolic acid-arabinogalactan-peptidoglycan complex (mAGP), interspersed with additional complex lipids which helps them persist in their host. The present study finds that poly(dimethylaminoethyl methacrylate) has particularly potent antimycobacterial activity and high selectivity over two Gram-negative strains. Removal of the backbone methyl group (poly(dimethylaminoethyl acrylate)) decreased antimycobacterial activity, and poly(aminoethyl methacrylate) also had no activity against mycobacteria. Hemolysis assays revealed poly(dimethylaminoethyl methacrylate) did not disrupt red blood cell membranes. Interestingly, poly(dimethylaminoethyl methacrylate) was not found to permeabilize mycobacterial membranes, as judged by dye exclusion assays, suggesting the mode of action is not simple membrane disruption, supported by electron microscopy analysis. These results demonstrate that synthetic polycations, with the correctly tuned structure are useful tools against mycobacterial infections, for which new drugs are urgently required

Petabyte-scale innovations at the European Nucleotide Archive

Dramatic increases in the throughput of nucleotide sequencing machines, and the promise of ever greater performance, have thrust bioinformatics into the era of petabyte-scale data sets. Sequence repositories, which provide the feed for these data sets into the worldwide computational infrastructure, are challenged by the impact of these data volumes. The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/embl), comprising the EMBL Nucleotide Sequence Database and the Ensembl Trace Archive, has identified challenges in the storage, movement, analysis, interpretation and visualization of petabyte-scale data sets. We present here our new repository for next generation sequence data, a brief summary of contents of the ENA and provide details of major developments to submission pipelines, high-throughput rule-based validation infrastructure and data integration approaches