Vasopressin attenuates ischemia-reperfusion injury via reduction of oxidative stress and inhibition of mitochondrial permeability transition pore opening in rat hearts

Aim of this study was to investigate the involvement of the mitochondrial permeability transition pore (MPTP) and oxidative stress in the cardioprotective effect of vasopressin (AVP) on ischemia/reperfusion (I/R) injury. Anesthetized male wistar rats were subjected to regional 30 min ischemia and 120 min reperfusion and randomly divided into nine groups: (1) Control; saline was administered intravenously before ischemia, (2) vasopressin was administrated 10 min prior to ischemia, (3, 4) Atractyloside as MPTP opener, was injected 5 min prior to reperfusion without and with vasopressin, (5, 6) Cyclosporine A as a MPTP closer, was injected 5 min prior to reperfusion without and with vasopressin, (7) mitochondria were isolated from control group and CaCl2 was added as MPTP opener and swelling inducer, (8) isolated mitochondria from Control hearts was incubated with Cyclosporine A before adding the CaCl2 (9) CaCl2 was added to isolated mitochondria from vasopressin group. Infusion of vasopressin decreased infarct size (18.6±1.7% vs. control group 37.6±2.4%), biochemical parameters [LDH (Lactate Dehydrogenase), CK-MB (Creatine Kinase-MB) and MDA (Malondialdehyde) plasma levels, PAB (Prooxidant-antioxidant balance)] compared to control group. Atactyloside suppressed the cardioprotective effect of vasopressin (32.5±1.9% vs. 18.6±1.7%) but administration of the Cyclosporine A without and with vasopressin significantly reduced infarct size to 17.7±4% (P<0.001) and 22.7±3% (P<0.01) respectively, vs. 37.6±2.4% in control group. Also, vasopressin, similar to Cyclosporine A, led to decrease in CaCl2-induced swelling. It seems that vasopressin through antioxidant effect and MPTP inhibition has created a cardioprotection against ischemia/reperfusion injuries. © 2015 Elsevier B.V. All rights reserved

Comparison of the effect of ginger and aloe vera mouthwashes on xerostomia in patients with type 2 diabetes:a clinical trial, triple-blind

Ginger and aloe vera are two medicinal herbs mostly used to produce mouthwash. This study aimed to compare the effects of ginger and aloe vera mouthwashes on the xerostomia in patients referred to Bandar Abbas diabetes clinic (Iran). In this triple-blind clinical trial, three groups (n=35 patients in each group) were studied. One group was given aloe vera mouthwash, the other group used ginger mouthwash, and the control group was only given normal saline. All the mouthwashes were used 20 ccs three times daily for 14 consecutive days. The symptoms and severity of xerostomia were measured before and after the intervention by the questionnaire proposed by Fox et al. as well as VAS scale, respectively. Statistical analysis, including the Shapiro-Wilk test used to measure the normality of variables, and Chi-square and McNemar used to compare the scores questionnaire proposed by Fox et al., were also applied. Scores of VAS were analyzed using a one-way ANOVA test. P<0.05 was considered as statistically significant in all these tests. The mean age of the participants included in the normal saline group was 58.13±14.75 years old, 54.14±9.35 years old in the ginger group, and 53.37±11.57 years old in the aloe vera group. The difference between the scores of xerostomia before and after performing the intervention (The amount of reduction in xerostomia) in the ginger group was 6.12±2.004 cm, in the aloe vera group it was 4.08±2.09 cm, and in the normal saline group it was 2.45±2.09 cm. Finally, the ginger and aloe vera mouthwashes significantly reduced all symptoms and severity related to xerostomia, respectively(p<0.001). The use of mouthwash is an effective measure to reduce xerostomia under chronic conditions. Aloe vera, ginger, and normal saline are known as effective mouthwashes on controlling this complication. According to the results of this study, ginger and aloe vera mouthwashes could significantly decrease xerostomia and promote oral health in patients with diabetes

The effect of automated telephone system on the satisfaction of client in the Imam Ali polyclinic

زمینه و هدف: رضایت بیمار شاخصی برای ارزیابی خدمات است و سنجش کارآیی و کیفیت خدمات ارائه&zwnj;شده به مردم در بخش بهداشت و درمان از اولویت&zwnj;های مهم وزارت بهداشت است. به همین منظور این مطالعه، باهدف بررسی تأثیر استقرار سامانه نوبت&zwnj;دهی تلفنی بر رضایت مراجعین پلی کلینیک فوق تخصصی امام علی (ع) شهرکرد انجام شد. روش بررسی: این مطالعه تحلیلی- مقطعی در پلی کلینیک تخصصی و فوق تخصصی امام علی(ع) شهرکرد در سال1392 انجام شد. پرسشنامه رضایت سنجی استاندارد با بررسی700 نفر از مراجعین این مرکز قبل و بعد از استقرار سامانه نوبت&zwnj;دهی تکمیل گردید. اطلاعات توسط شاخص&zwnj;های آماری توصیفی و استنباطی با نرم&zwnj;افزار SPSS نسخه 16 مورد تجزیه &zwnj;و تحلیل قرار گرفت. یافته&zwnj;ها: میانگین امتیاز رضایت مراجعه&zwnj;کنندگان در سال 1391، معادل 7/5&plusmn;5/22 و در سال 1392 ،3/6&plusmn;7/23 بود که تفاوت 2 سال ازنظر آماری نشانگر ارتباط معنی دار پس از استقرار سیستم مکانیزه نوبت&zwnj;دهی بود (002/0=P). شاخص&zwnj;های سیستم نوبت&zwnj;دهی، نحوه دریافت نوبت، مکان انتظار، نظافت و تمیزی کلینیک، برخورد نگهبانی، برخورد نیروی پذیرش و منشی، برخورد پزشک، نحوه ویزیت پزشک، رعایت حریم شخصی و انتخاب کلینیک برای مراجعه بعدی در مجموع 78 رضایت&zwnj;مندی بیماران را تبیین می&zwnj;کردند. نتیجه&zwnj;گیری: اصلاح فرایندهایی مثل نوبت&zwnj;دهی باعث افزایش رضایتمندی بیماران می&zwnj;گردد، لذا بررسی منظم و دوره&zwnj;ای میزان رضایتمندی بیماران و ارائه برنامه&zwnj;های عملیاتی برای رفع نارضایتی آنان می&zwnj;تواند منجر به ارتقاء کیفیت خدمات بهداشتی درمانی گردد

Detection of Sea, Seb, Sec, Seq genes in staphylococcus aureus isolated from nasal carriers in Tehran province, Iran; by multiplex PCR

Staphylococcus(S.) aureus  produces different extra-cellular protein toxins and virulence factors. One of the most important extra-cellular proteins is an enterotoxin which causes staphylococcal food poisoning (SFP) due to their enterotoxins. Different methods have been used to detect this toxin, each of which has advantages and disadvantages. DNA amplification methods, however, can show the presence of enterotoxigenic strains of S. aureus before the expression of enterotoxins on the basis of specific gene sequences. In this study, 150 S. aureus strains isolated from nasal carriers were confirmed by biochemical testing. PCR was used to amplify the staphylococcal enterotoxin A, B, C and Q genes, as well as the staphylococcal nuclease gene.  Among the 150 healthy human isolates from the nasal carrier, 95 were confirmed as S. aureus.  Only 58.9% of the isolates were diagnosed as sea, b, c, q positive. There were 24 (25.3%) isolates associated with the sea gene, 15.8% isolates associated with the seb gene, 9.5% of the isolates were associated with the sec gene, and 8.4% of the isolates associated with the seq gene. Of these isolates, 41% might be possessing additional se genes but they were not see (178 bp) and sed (319 bp) genes.  The nuc gene, which encodes thermo nuclease, was used as a target DNA to identify S. aureus. Additionally, one of these enterotoxigenic isolates carried more than one toxin gene

The emerging role of miR-20b in human cancer and other disorders: Pathophysiology and therapeutic implications

miR-20b is a microRNA with diverse and somehow contradictory roles in the pathogenesis of human disorders, especially cancers. It has been known to be a tumor suppressor in colon cancer, renal cell carcinoma, prostate cancer, osteosarcoma and papillary thyroid cancer. In lung cancer and breast cancers, both tumor suppressor and oncogenic effects have been identified for this miRNA. Finally, in T cell leukemia, hepatocellular carcinoma, esophageal squamous cell carcinoma and cervical and gastric cancers, miR-20b is regarded as an oncogenic miRNA. In several types of cancer, dysregulation of miR-20b has been recognized as a predictive marker for patients’ survival. Dysregulation of miR-20b has also been recognized in Alzheimer’s disease, diabetic retinopathy, myocardial ischemia/infarction, chronic hepatitis B and multiple sclerosis. In the current review, we have summarized the miR-20b targets and related cellular processes. We have also provided a review of participation of this miRNA in different human disorders

Overexpression of Metastatic Related MicroRNAs, Mir-335 and Mir-10b, by Staphylococcal Enterotoxin B in the Metastatic Breast Cancer Cell Line

Purpose : One of the advanced cancer therapy strategies is immune - stimulating compound based immunotherapy Staphylococcal enterotoxin B (SEB) is one of the potent superantigens, which can efficiently activate antitumor immune response to eradicate tumor growth and inhibit metastasis. Herein, we evaluated the effect of SEB on the expression of two master microRN As, mir - 335 and mir - 10b, involved in metastasis. Methods : A metastatic breast cancer cell line MDA - MB231was treated with four different concentrations of SEB, including 10, 10 2 , 10 3 and 10 4 ng/ml, for 24 and 48 hours. To identify the cytotoxic effect of S EB, treated cells were examined by MTT assay. The stem loop RT - PCR (TaqMan) was used to analyze the mir - 335 and mir - 10b expression. Results : Results showed that SEB significantly increased the expression of mir - 335 both after 24 and 48 hours ( p v < 0.001 and p v < 0.05, respectively). No significant differences were found in the mir - 10b expression. Conclusion : Moreover, our findings demonstrated no cytotoxic effect of SEB on the treated cells. Our results suggest that SEB probably induces its anti - m etastatic effect via the expression regulation of the main genes which contributes to metastasis

Interleukin 4 gene polymorphism (−589C/T) and the risk of asthma: a meta-analysis and met-regression based on 55 studies

Background: Numerous investigations have previously evaluated the association of interleukin (IL) 4 gene polymorphisms and the risk of asthma, conferring inconsistent results. To resolve the incongruent outcomes yielded from different single studies, we conducted the most up-to-date meta-analysis of IL4 gene −589C/T (rs2243250) polymorphism and susceptibility to asthma. Methods: A systematic literature search was performed in ISI web of science, Scopus, Medline/PubMed databases prior to September 2020, and the pooled odds ratio (OR) and their corresponding 95% CI were calculated to determine the association strength. Results: Literature search led to retrieving of 49 publications (55 case-control studies) containing 9572 cases and 9881 controls. It was revealed that IL4 gene −589C/T polymorphism increased the risk of asthma across all genetic models, including dominant model (OR = 1.22), recessive model (OR = 1.17), allelic model (OR = 1.21), and TT vs. CC model (OR = 1.34), but not the CT vs. TT model. The subgroup analysis by age indicated that IL4 gene -589C/T polymorphism was significantly associated with asthma risk in both pediatrics and adults. Additionally, the subgroup analysis by ethnicity revealed significant association in Asian, American, and Europeans. Finally, subgroup analysis by East Asian and non-East Asian populations indicated significant associations. Conclusions: The current meta-analysis revealed that IL4 gene -589C/T polymorphism was a susceptibility risk in both pediatrics and adults in the whole and different ethnic groups