Vegetable Production and Pesticide Use in Ghana: Would GM Varieties Have an Impact at the Farm Level?

The objective of this study is to evaluate pesticide use as an important factor affecting potential adoption and farm level impact of genetically modified (GM) vegetable varieties in Ghana. Tomato is the most consumed vegetable and a food security crop. Cabbage is a vegetable of growing importance but limited cultivation and is produced in urban areas. Garden egg is a native African crop of wide consumption and importance for rural economies. Farm level information was collected in randomly selected sites in southern and central regions of Ghana. Partial budget analysis shows that investments in pesticides are rather low, especially for tomato and garden egg. Analysis of production using an abatement framework shows that insecticide amounts are significant in determining cabbage output levels only. Rate of returns of GM seeds however can still be high. GM varieties would need to show not only a high abatement rate and a high yield potential but mainly an affordable price, to reduce total costs and induce adoption.Farm, Genetically Modified, Ghana, Tomato, Agricultural and Food Policy, Consumer/Household Economics, Environmental Economics and Policy, Food Consumption/Nutrition/Food Safety, Food Security and Poverty, Health Economics and Policy, International Relations/Trade, Marketing, Productivity Analysis, Research and Development/Tech Change/Emerging Technologies,

Insecticide Use on Vegetables in Ghana: Would GM Seed Benefit Farmers?

Tomato, cabbage and garden egg (African eggplant, or Solanum Aethiopicum) are important crops for small-scale farmers and migrants in the rural and peri-urban areas of Ghana. Genetic modification (GM) has the potential to alleviate poverty through combating yield losses from pests and diseases in these crops, while reducing health risks from application of hazardous chemicals. This ex-ante study uses farm survey data to gauge the potential for adoption of genetically-engineered varieties, estimate the potential impact of adoption on farm profits, and highlight economic differences among the three crops. Farmer's expenditures on insecticides are below the economic optimum in all three crops, and the estimated function for damage abatement shows that insecticide amounts are significant determinants of cabbage yields only. Nonetheless, yield losses from the pests and diseases affect insecticide use. Stochastic budget analysis also indicates a higher rate of return to vegetable production with the use of resistant seeds relative to status quo, even considering the technology transfer fee for GM seeds. Non-insecticide users could accrue higher marginal benefits than current insecticide users. Comparing among vegetable crops with distinct economic characteristics provides a wider perspective on the potential impact of GM technology. Until now, GM eggplant is the only vegetable crop that has been analyzed in the peer-reviewed, applied economics literature. This is the first analysis that includes African eggplant.Crop Production/Industries, Research and Development/Tech Change/Emerging Technologies,

From "best practice" to "best fit": a framework for designing and analyzing pluralistic agricultural advisory services

"Agricultural advisory services play an important role in supporting the use of the agricultural sector as an engine of pro-poor growth and enabling small farmers to meet new challenges, such as accessing export markets, adopting environmentally sustainable production techniques, and coping with HIV/AIDS and other health challenges that affect agriculture...There is now renewed interest in agricultural advisory services in many countries.... The questions under debate include: What should be the roles of the public sector, private sector, and civil society? How can we ensure that agricultural advisory services are demand-driven and meet the diverse information needs of farmers? How can advisory services be made efficient and financially sustainable? How can we ensure that female farmers, the poor, and other marginalized groups have access to agricultural advisory services?" from Authors' SummaryAgricultural extension work, Pro-poor growth, Capacity strengthening,

Evaluation of methods for DNA extraction from Clostridium tyrobutyricum spores and its detection by qPCR

Clostridium tyrobutyricum is the major agent that causes the blowing defect in cheese due to the germination of its dormant spores during the ripening stage. As a result, many of the affected cheeses show cavities and cracks, which cause the product loss in most cases. Nowadays, there is not a fast method capable of detecting milk contaminated with C. tyrobutyricum spores. The aim of this study has been to develop a fast and reliable method based on real time PCR (qPCR) to detect C. tyrobutyricum spores in raw milk. One of the main limitations has been to find a good procedure for the spore disruption to extract the DNA due to its high resistance. For this reason, different disruption methods have been tested, including chemical agents, bead beating, enzymatic and microwave treatment. Furthermore, an enzymatic treatment with subtilisin was applied for milk clarification and recovery of spores. The comparison of the assayed methods has been made using sterile milk spiked with C. tyrobutyricum spores, obtained in solid or liquid medium. The results showed that microwave treatment followed by a standard DNA purification step was found to be the best disruption method. The Ct values obtained for spores were higher than those found for vegetative cells by qPCR, for the same quantity of DNA. This difference could be due to the action of the Small Acid Soluble Proteins (SASP) in the DNA packaging of spores. Moreover, spores obtained in agar plate were found more resistant to disruption than those obtained in liquid medium. Subtilisin and microwave treatments were found to be successful for DNA extraction from C. tyrobutyricum spores in milk and subsequent identification by qPCR. However, the differences observed between the amplification of DNA from spores obtained in different media and from vegetative cells have to be taken into account to optimize a method for C. tyrobutyricum detection

SEROPREVALENCIA DE Neospora caninum EN CANINOS DE DOS DISTRITOS DE LA PROVINCIA DE CHACHAPOYAS

El Neospora caninum, parásito del canino, es ampliamente conocido como causante de abortos y mortalidad neonatal en bovinos a nivel mundial. El objetivo del presente estudio fue determinar la seroprevalencia de N. caninum en caninos de los distritos de Molinopampa y Leymebamba, provincia de Chachapoyas, Amazonas. Se evaluaron 142 sueros de caninos (63 de Molinopampa y 79 de Leymebamba) para la detección de anticuerpos mediante la prueba de Inmufluorescencia Indirecta (IFI). El 28.9 ± 7.5% de los caninos presentaron anticuerpos contra N. caninum. Seroprevalencias de 34.9 ± 11.8% y 24.1 ± 9.4% fueron halladas en caninos de los distritos de Molinopampa y Leymebamaba, respectivamente. Estos resultados demuestran la existencia de una seroprevalencia moderadamente alta de caninos infectados con N. caninum en la región estudiada.Neospora caninum is a parasite of dogs that causes high rates of abortion and neonatal mortality in cattle throughout the world. The objective of the study was to determine the prevalence of Neospora caninum in dogs from districts of Molinopampa and Leymebamba, province of Chachapoyas, Amazonas. A total of 142 serum samples were evaluated (63 from Molinopampa and 79 from Leymebamba) for the detection of antibodies using the indirect immunofluorescence test (IFAT). The 28.9 ± 7.5% of the animals were positive to N. caninum. A seroprevalence of 34.9 ± 11.8% and 24.1 ± 9.4% were found in dogs of Molinopampa and Leymebamba districts, respectively. This results demonstrate the existence of a high seroprevalence of dogs infected with Neospora caninum in the region

Blue-green to near-IR switching electroluminescence from Si-rich silicon oxide/nitride bilayer structures

Blue green to near-IR switching electroluminescence (EL) has been achieved in a metal-oxide-semiconductor light emitting device, where the dielectric has been replaced by a Si-rich silicon oxide/nitride bilayer structure. To form Si nanostructures, the layers were implanted with Si ions at high energy, resulting in a Si excess of 19%, and subsequently annealed at 1000 °C. Transmission electron microscopy and EL studies allowed ascribing the blue-green emission to the Si nitride related defects and the near-IR band with the emission of the Si-nanoclusters embedded into the SiO2 layer. Charge transport analysis is reported and allows for identifying the origin of this twowavelength switching effect

Multiple approaches at admission based on lung ultrasound and biomarkers improves risk identification in COVID-19 patients

Background: Risk stratification of COVID-19 patients is fundamental to improving prognosis and selecting the right treatment. We hypothesized that a combination of lung ultrasound (LUZ-score), biomarkers (sST2), and clinical models (PANDEMYC score) could be useful to improve risk stratification. Methods: This was a prospective cohort study designed to analyze the prognostic value of lung ultrasound, sST2, and PANDEMYC score in COVID-19 patients. The primary endpoint was in-hospital death and/or admission to the intensive care unit. The total length of hospital stay, increase of oxygen flow, or escalated medical treatment during the first 72 h were secondary endpoints. Results: a total of 144 patients were included; the mean age was 57.5 ± 12.78 years. The median PANDEMYC score was 243 (52), the median LUZ-score was 21 (10), and the median sST2 was 53.1 ng/mL (30.9). Soluble ST2 showed the best predictive capacity for the primary endpoint (AUC = 0.764 (0.658–0.871); p = 0.001), towards the PANDEMYC score (AUC = 0.762 (0.655–0.870); p = 0.001) and LUZ-score (AUC = 0.749 (0.596–0.901); p = 0.002). Taken together, these three tools significantly improved the risk capacity (AUC = 0.840 (0.727–0.953); p = 0.001). Conclusions: The PANDEMYC score, lung ultrasound, and sST2 concentrations upon admission for COVID-19 are independent predictors of intra-hospital death and/or the need for admission to the ICU for mechanical ventilation. The combination of these predictive tools improves the predictive power compared to each one separately. The use of decision trees, based on multivariate models, could be useful in clinical practice. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)

Foodways in transition: food plants, diet and local perceptions of change in a Costa Rican Ngäbe community

Background Indigenous populations are undergoing rapid ethnobiological, nutritional and socioeconomic transitions while being increasingly integrated into modernizing societies. To better understand the dynamics of these transitions, this article aims to characterize the cultural domain of food plants and analyze its relation with current day diets, and the local perceptions of changes given amongst the Ngäbe people of Southern Conte-Burica, Costa Rica, as production of food plants by its residents is hypothesized to be drastically in recession with an decreased local production in the area and new conservation and development paradigms being implemented. Methods Extensive freelisting, interviews and workshops were used to collect the data from 72 participants on their knowledge of food plants, their current dietary practices and their perceptions of change in local foodways, while cultural domain analysis, descriptive statistical analyses and development of fundamental explanatory themes were employed to analyze the data. Results Results show a food plants domain composed of 140 species, of which 85 % grow in the area, with a medium level of cultural consensus, and some age-based variation. Although many plants still grow in the area, in many key species a decrease on local production–even abandonment–was found, with much reduced cultivation areas. Yet, the domain appears to be largely theoretical, with little evidence of use; and the diet today is predominantly dependent on foods bought from the store (more than 50 % of basic ingredients), many of which were not salient or not even recognized as ‘food plants’ in freelists exercises. While changes in the importance of food plants were largely deemed a result of changes in cultural preferences for store bought processed food stuffs and changing values associated with farming and being food self-sufficient, Ngäbe were also aware of how changing household livelihood activities, and the subsequent loss of knowledge and use of food plants, were in fact being driven by changes in social and political policies, despite increases in forest cover and biodiversity. Conclusions Ngäbe foodways are changing in different and somewhat disconnected ways: knowledge of food plants is varied, reflecting most relevant changes in dietary practices such as lower cultivation areas and greater dependence on food from stores by all families. We attribute dietary shifts to socioeconomic and political changes in recent decades, in particular to a reduction of local production of food, new economic structures and agents related to the State and globalization