Introduction: new directions in energy demand research

Meeting the goals enshrined in the Paris Agreement and limiting global temperature increases to less than 2°C above pre-industrial levels demands rapid reductions in global carbon dioxide emissions. Reducing energy demand has a central role in achieving this goal, but existing policy initiatives have been largely incremental in terms of the technological and behavioural changes they encourage. Against this background, this book develops a sociotechnical approach to the challenge of reducing energy demand and illustrates this with a number of empirical case studies from the United Kingdom. In doing so, it explores the emergence, diffusion and impact of low-energy innovations. This chapter introduces the main themes of the book, including explorations of the processes and mechanisms through which different types of innovations become (or fail to become) established, the identification of the role of different groups, assessments of the resulting impacts on energy demand and other social goals, and the development of recommendations for both encouraging the diffusion of such innovations and maximising their long-term impact

Imagining sustainable energy and mobility transitions: valence, temporality, and radicalism in 38 visions of a low-carbon future

Based on an extensive synthesis of semi-structured interviews, media content analysis, and reviews, this article conducts a qualitative meta-analysis of more than 560 sources of evidence to identify 38 visions associated with seven different low-carbon innovations – automated mobility, electric vehicles, smart meters, nuclear power, shale gas, hydrogen, and the fossil fuel divestment movement – playing a key role in current deliberations about mobility or low-carbon energy supply and use. From this material, it analyzes such visions based on rhetorical features such as common problems and functions, storylines, discursive struggles, and rhetorical effectiveness. It also analyzes visions based on typologies or degrees of valence (utopian vs. dystopian), temporality (proximal vs. distant), and radicalism (incremental vs. transformative). The article is motivated by the premise that tackling climate change via low-carbon energy systems (and practices) is one of the most significant challenges of the twenty-first century, and that effective decarbonization will require not only new energy technologies, but also new ways of understanding language, visions, and discursive politics surrounding emerging innovations and transitions

Africa Radiation Oncology Network (AFRONET): an IAEA Telemedicine Pilot Project

In developing countries, many centres work in relative isolation with limited access to up-to-date published literature, international meetings, and expert opinion. Methods: The International Atomic Energy Agency established the AFrica Radiation Oncology NETwork (AFRONET) as a pilot project for African countries. Through videoconferencing, cancer professionals discussed and reviewed challenging cancer cases. Monthly virtual meetings took place among radiotherapy centres in Africa. During these meetings, individual cases were presented and discussed, and a consensus recommendation for treatment was reached. The platform was also used for regularly scheduled webinars. Results: 64 monthly meetings were held and 154 cases have been discussed. The average number of participating centres was 9 per session. Central nervous system tumours were the most common and 5.2% of patients were HIV positive. The profile of diseases and comorbidities represents a window into the typical patient population of radiotherapy centres in Africa. Videoconferencing discussions strengthened clinical decision making for oncology patients. Both the case discussions and the webinars contributed to resident education in participating centres. Conclusions: This pilot experience has shown that it is feasible to use available telemedicine tools to establish a network for case discussions and education in radiation oncology in African countries. &nbsp

Eating, heating or taking the bus? Lived experiences at the intersection of energy and transport poverty

Experiences of poverty can manifest in multiple aspects of everyday life, often in interlinking ways. One example is ‘double energy vulnerability’, where a household faces both energy poverty and transport poverty simultaneously. This can result in trade-offs, where prioritising one essential need (e.g., transport) makes accessing another impossible (e.g., heating). Such decisions are not easily made, and they can have distinct spatio-temporal characteristics. They can vary between space and time and across different household members, and result in stark inter- as well as intra-household differences. People with socio-demographic and contextual vulnerabilities are particularly at risk of experiencing double energy vulnerability. Based on 59 household interviews across the four nations of the United Kingdom, we provide novel, multi-nation empirical evidence on the lived experiences of double energy vulnerability, drawing on our themes; ‘being locked into infrastructure’, ‘facing high costs and low incomes’, ‘choosing between energy and transport’, and ‘missing out’. A cross-national lived-experiences approach sheds light on double energy vulnerability as a relational, contingent and ongoing phenomena, attending to everyday experiences and capacities. We provide suggestions for further research, such as further study of double energy vulnerability amongst refugees and migrants. We also highlight that the study of lived experiences can aid the recognition of how different forms of poverty intersect and how they need to be taken into account in the design of Net Zero policies

Myosin II regulates the shape of three-dimensional intestinal epithelial cysts.

The development of luminal organs begins with the formation of spherical cysts composed of a single layer of epithelial cells. Using a model three-dimensional cell culture, this study examines the role of a cytoskeletal motor, myosin II, in cyst formation. Caco-2 and SK-CO15 intestinal epithelial cells were embedded into Matrigel, and myosin II was inhibited by blebbistatin or siRNA-mediated knockdown. Whereas control cells formed spherical cysts with a smooth surface, inhibition of myosin II induced the outgrowth of F-actin-rich surface protrusions. The development of these protrusions was abrogated after inhibition of F-actin polymerization or of phospholipase C (PLC) activity, as well as after overexpression of a dominant-negative ADF/cofilin. Surface protrusions were enriched in microtubules and their formation was prevented by microtubule depolymerization. Myosin II inhibition caused a loss of peripheral F-actin bundles and a submembranous extension of cortical microtubules. Our findings suggest that inhibition of myosin II eliminates the cortical F-actin barrier, allowing microtubules to reach and activate PLC at the plasma membrane. PLC-dependent stimulation of ADF/cofilin creates actin-filament barbed ends and promotes the outgrowth of F-actin-rich protrusions. We conclude that myosin II regulates the spherical shape of epithelial cysts by controlling actin polymerization at the cyst surface

Impact of changing climate on bryophyte contributions to terrestrial water, carbon, and nitrogen cycles

Bryophytes, including the lineages of mosses, liverworts, and hornworts, are the second-largest photoautotroph group on Earth. Recent work across terrestrial ecosystems has highlighted how bryophytes retain and control water, fix substantial amounts of carbon (C), and contribute to nitrogen (N) cycles in forests (boreal, temperate, and tropical), tundra, peatlands, grasslands, and deserts. Understanding how changing climate affects bryophyte contributions to global cycles in different ecosystems is of primary importance. However, because of their small physical size, bryophytes have been largely ignored in research on water, C, and N cycles at global scales. Here, we review the literature on how bryophytes influence global biogeochemical cycles, and we highlight that while some aspects of global change represent critical tipping points for survival, bryophytes may also buffer many ecosystems from change due to their capacity for water, C, and N uptake and storage. However, as the thresholds of resistance of bryophytes to temperature and precipitation regime changes are mostly unknown, it is challenging to predict how long this buffering capacity will remain functional. Furthermore, as ecosystems shift their global distribution in response to changing climate, the size of different bryophyte-influenced biomes will change, resulting in shifts in the magnitude of bryophyte impacts on global ecosystem functions

Many Ribosomal Protein Genes Are Cancer Genes in Zebrafish

We have generated several hundred lines of zebrafish (Danio rerio), each heterozygous for a recessive embryonic lethal mutation. Since many tumor suppressor genes are recessive lethals, we screened our colony for lines that display early mortality and/or gross evidence of tumors. We identified 12 lines with elevated cancer incidence. Fish from these lines develop malignant peripheral nerve sheath tumors, and in some cases also other tumor types, with moderate to very high frequencies. Surprisingly, 11 of the 12 lines were each heterozygous for a mutation in a different ribosomal protein (RP) gene, while one line was heterozygous for a mutation in a zebrafish paralog of the human and mouse tumor suppressor gene, neurofibromatosis type 2. Our findings suggest that many RP genes may act as haploinsufficient tumor suppressors in fish. Many RP genes might also be cancer genes in humans, where their role in tumorigenesis could easily have escaped detection up to now

Clathrin- and Dynamin-Independent Endocytosis of FGFR3 – Implications for Signalling

Endocytosis of tyrosine kinase receptors can influence both the duration and the specificity of the signal emitted. We have investigated the mechanisms of internalization of fibroblast growth factor receptor 3 (FGFR3) and compared it to that of FGFR1 which is internalized predominantly through clathrin-mediated endocytosis. Interestingly, we observed that FGFR3 was internalized at a slower rate than FGFR1 indicating that it may use a different endocytic mechanism than FGFR1. Indeed, after depletion of cells for clathrin, internalization of FGFR3 was only partly inhibited while endocytosis of FGFR1 was almost completely abolished. Similarly, expression of dominant negative mutants of dynamin resulted in partial inhibition of the endocytosis of FGFR3 whereas internalization of FGFR1 was blocked. Interfering with proposed regulators of clathrin-independent endocytosis such as Arf6, flotillin 1 and 2 and Cdc42 did not affect the endocytosis of FGFR1 or FGFR3. Furthermore, depletion of clathrin decreased the degradation of FGFR1 resulting in sustained signalling. In the case of FGFR3, both the degradation and the signalling were only slightly affected by clathrin depletion. The data indicate that clathrin-mediated endocytosis is required for efficient internalization and downregulation of FGFR1 while FGFR3, however, is internalized by both clathrin-dependent and clathrin-independent mechanisms