44 research outputs found
A nomogram to predict in-hospital mortality of gastrointestinal bleeding patients in the intensive care unit
BackgroundGastrointestinal bleeding (GIB) is a common condition in clinical practice, and predictive models for patients with GIB have been developed. However, assessments of in-hospital mortality due to GIB in the intensive care unit (ICU), especially in critically ill patients, are still lacking. This study was designed to screen out independent predictive factors affecting in-hospital mortality and thus establish a predictive model for clinical use.MethodsThis retrospective study included 1,442 patients with GIB who had been admitted to the ICU. They were selected from the Medical Information Mart for Intensive Care IV (MIMIC-IV) 1.0 database and divided into a training group and a validation group in a ratio of 7:3. The main outcome measure was in-hospital mortality. Least absolute shrinkage and section operator (LASSO) regression was used to screen out independent predictors and create a nomogram.ResultsLASSO regression picked out nine independent predictors: heart rate (HR), activated partial thromboplastin time (aPTT), acute physiology score III (APSIII), sequential organ failure assessment (SOFA), cerebrovascular disease, acute kidney injury (AKI), norepinephrine, vasopressin, and dopamine. Our model proved to have excellent predictive value with regard to in-hospital mortality (the area under the receiver operating characteristic curve was 0.906 and 0.881 in the training and validation groups, respectively), as well as a good outcome on a decision curve analysis to assess net benefit.ConclusionOur model effectively predicts in-hospital mortality in patients with GIB, indicating that it may prove to be a valuable tool in future clinical practice
Mapping and functional characterization of structural variation in 1060 pig genomes
BACKGROUND: Structural variations (SVs) have significant impacts on complex phenotypes by rearranging large amounts of DNA sequence.RESULTS: We present a comprehensive SV catalog based on the whole-genome sequence of 1060 pigs (Sus scrofa) representing 101 breeds, covering 9.6% of the pig genome. This catalog includes 42,487 deletions, 37,913 mobile element insertions, 3308 duplications, 1664 inversions, and 45,184 break ends. Estimates of breed ancestry and hybridization using genotyped SVs align well with those from single nucleotide polymorphisms. Geographically stratified deletions are observed, along with known duplications of the KIT gene, responsible for white coat color in European pigs. Additionally, we identify a recent SINE element insertion in MYO5A transcripts of European pigs, potentially influencing alternative splicing patterns and coat color alterations. Furthermore, a Yorkshire-specific copy number gain within ABCG2 is found, impacting chromatin interactions and gene expression across multiple tissues over a stretch of genomic region of ~200 kb. Preliminary investigations into SV's impact on gene expression and traits using the Pig Genotype-Tissue Expression (PigGTEx) data reveal SV associations with regulatory variants and gene-trait pairs. For instance, a 51-bp deletion is linked to the lead eQTL of the lipid metabolism regulating gene FADS3, whose expression in embryo may affect loin muscle area, as revealed by our transcriptome-wide association studies.CONCLUSIONS: This SV catalog serves as a valuable resource for studying diversity, evolutionary history, and functional shaping of the pig genome by processes like domestication, trait-based breeding, and adaptive evolution.</p
Probing high-momentum component in nucleon momentum distribution by neutron-proton bremsstrahlung {\gamma}-rays in heavy ion reactions
The high momentum tail (HMT) of nucleons, as a signature of the short-range
correlations in nuclei, has been investigated by the high-energy bremsstrahlung
rays produced in Kr + Sn at 25 MeV/u. The energetic
photons are measured by a CsI(Tl) hodoscope mounted on the spectrometer CSHINE.
The energy spectrum above 30 MeV can be reproduced by the IBUU model
calculations incorporating the photon production channel from process in
which the HMTs of nucleons is considered. A non-zero HMT ratio of about
is favored by the data. The effect of the capture channel is
demonstrated
Revisit to the yield ratio of triton and He as an indicator of neutron-rich neck emission
The neutron rich neck zone created in heavy ion reaction is experimentally
probed by the production of the isobars. The energy spectra and angular
distributions of triton and He are measured with the CSHINE detector in
Kr +Pb reactions at 25 MeV/u. While the energy spectrum of
He is harder than that of triton, known as "He-puzzle", the yield
ratio presents a robust rising trend with the polar angle in
laboratory. Using the fission fragments to reconstruct the fission plane, the
enhancement of out-plane is confirmed in comparison to the
in-plane ratios. Transport model simulations reproduce qualitatively the
experimental trends, but the quantitative agreement is not achieved. The
results demonstrate that a neutron rich neck zone is formed in the reactions.
Further studies are called for to understand the clustering and the isospin
dynamics related to neck formation
A compendium of genetic regulatory effects across pig tissues
The Farm Animal Genotype-Tissue Expression (FarmGTEx) project has been established to develop a public resource of genetic regulatory variants in livestock, which is essential for linking genetic polymorphisms to variation in phenotypes, helping fundamental biological discovery and exploitation in animal breeding and human biomedicine. Here we show results from the pilot phase of PigGTEx by processing 5,457 RNA-sequencing and 1,602 whole-genome sequencing samples passing quality control from pigs. We build a pig genotype imputation panel and associate millions of genetic variants with five types of transcriptomic phenotypes in 34 tissues. We evaluate tissue specificity of regulatory effects and elucidate molecular mechanisms of their action using multi-omics data. Leveraging this resource, we decipher regulatory mechanisms underlying 207 pig complex phenotypes and demonstrate the similarity of pigs to humans in gene expression and the genetic regulation behind complex phenotypes, supporting the importance of pigs as a human biomedical model.</p
Substantially Enhanced Stereocomplex Crystallization of Poly(L-lactide)/Poly(D-lactide) Blends by the Formation of Multi-Arm Stereo-Block Copolymers
Stereocomplex-type polylactide (SC-PLA) created by alternate packing of poly(L-lactide) (PLLA) and poly(D-lactide) (PDLA) chains in a crystalline state has emerged as a growingly popular engineering bioplastic that possesses excellent hydrolytic stability and thermomechanical properties. However, it is extremely difficult to acquire high-performance SC-PLA products via melt-processing of high-molecular-weight PLLA/PDLA blends because both SC crystallites and homocrystallites (HCs) are competitively formed in the melt-crystallization. Herein, a facile yet powerful way was employed to boost SC formation by introducing trace amounts of some epoxy-functionalized small-molecule modifiers into the enantiomeric blends during reactive melt-blending. The results show that the SC formation is considerably enhanced with the in situ generation of multi-arm stereo-block PLA copolymers, based on the reaction between epoxy groups of the modifiers and hydroxyl end groups of PLAs. More impressively, it is intriguing to find that the introduction of only 0.5 wt% modifiers can induce exclusive SC formation in the blends upon isothermal and non-isothermal melt-crystallizations. The outstanding SC crystallizability might be attributed to the suppressing effect of such unique copolymers on the separation of the alternately arranged PLLA/PDLA chain segments in molten state as a compatibilizer. Furthermore, the generation of these copolymers does not result in a significant increase in melt viscosity of the blends. These findings suggest new opportunities for the high-throughput processing of SC-PLA materials into useful products
Transcriptome analysis reveals association of carotenoid metabolism pathway with fruit color in melon
Abstract Flesh color is an important quality of melon (Cucumis melo L.) and is determined mainly by carotenoid content, awarding them with colors, aromas, and nutrients. enhancing the nutritional and health benefits of fruits and vegetables for humans. In this study, we performed transcriptomic analysis of two melon inbred line “B-14” (orange-flesh) and “B-6” (white-flesh) at three developmental stages. We observed that the β-carotene content of inbred line “B-6” (14.232 μg/g) was significantly lower than that of inbred line “B-14” (0.534 μg/g). RNA-sequencing and quantitative reverse transcription PCR analyses were performed to identify differentially expressed genes (DEGs) between the two inbred lines at different stages; the DEGs were analyzed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes databases (KEGG). We identified 33 structural DEGs in different developmental periods of the two lines that were related to carotenoid metabolism. Among them, PSY, Z-ISO, ZDS, CRTISO, CCD4, VDE1, and NCED2 were highly correlated with carotenoid content. Thus, this study provides a basis for molecular mechanism of carotenoid biosynthesis and flesh color in melon fruit
Comprehensive Evaluation on Urban Water Security Based on the Novel DPEBLR Concept Model and the Cloud Matter-Element Model: A Case Study of Chongqing, China
Urban water security problems such as water scarcity, water pollution, and flood disasters have restricted the sustainable development of cities. In order to scientifically evaluate the urban water security situation, this study combined the DPSIR (driver, pressure, state, impact, response) model and HEVD (the hazard of disaster-causing factors, the vulnerability of disaster-affected bodies, the instability of the disaster-pregnant environment, and population loss) model to propose a new conceptual framework for DPEBLR (driver, pressure, environment, insecurity-affected body, loss, response). Based on this conceptual framework, 35 indicators were selected to establish an urban water security evaluation index system. In order to solve the problem of randomness and fuzziness of the boundary values of urban water security classification levels, the cloud matter element model was applied. Chongqing was used as an example for verification, and the results showed that the water security status of Chongqing City improved from 2011 (Ⅴ) to 2019 (Ⅱ). This indicates that the water ecology restoration project, centralized water source protection project, centralized water supply project, water-saving society transformation, and flood and drought prevention system construction project implemented in Chongqing has achieved significant results. However, Chongqing still faces the problem that the construction of an urban flood control system cannot meet the speed of urban development. The government should slow down the urbanization and allow the urban flood control system to be ready before the area is occupied. In addition, the awareness of water conservation for industrial use in Chongqing has been formed, and citizens’ awareness of water conservation for domestic use should be enhanced in the future
Structural Characterization of β‑Agostic Bonds in Pd-Catalyzed Polymerization
β-agostic Pd
complexes are critical intermediates in catalytic
reactions, such as olefin polymerization and Heck reactions. Pd β-agostic
complexes, however, have eluded structural characterization, due to
the fact that these highly unstable molecules are difficult to isolate.
Herein, we report the single-crystal X-ray and neutron diffraction
characterization of β-agostic (α-diimine)ÂPd–ethyl
intermediates in polymerization. Short C<sub>α</sub>–C<sub>β</sub> distances and acute Pd–C<sub>α</sub>–C<sub>β</sub> bond angles combined serve as unambiguous evidence
for the β-agostic interaction. Characterization of the agostic
structure and the kinetic barrier for β-H elimination offer
important insight into the fundamental understanding of agostic bonds
and the mechanism of polymerization