1,132 research outputs found
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Production and uptake of dissolved carbon, nitrogen, and phosphorus in overlying water of aquaculture shrimp ponds in subtropical estuaries, China
Water quality deterioration can adversely affect the long-term sustainability of aquaculture industry. Understanding the processes of nutrient regeneration and uptake is important for improving water quality and the overall ecosystem health of aquaculture system. In spite of the importance of dissolved nutrients (DOC, DIC, N-NO , N-NH , and P-PO ) in governing water quality and ecosystem functioning, the spatiotemporal variations in the production and uptake of dissolved nutrients in aquaculture ponds is still poorly understood. In this study, the nutrient production and uptake rates in the overlying water were quantified among different shrimp growth stages in the aquaculture ponds in the Min River Estuary (MRE) and Jiulong River Estuary (JRE), southeast China. Significant differences in the nutrient production and uptake rates in the overlying water were observed among the three growth stages and two estuaries. The temporal variations of DOC and DIC production rates in both estuarine ponds closely followed the seasonal cycle of temperature, while the difference in DOC and DIC production rates between the two estuaries was likely caused by differences in water salinity. The changes in the production and uptake rates of dissolved inorganic nitrogen (N-NO and N-NH ) and P-PO in the water column over time were partly related to the interactions between thermal conditions and phytoplankton biomass (e.g., chlorophyll a concentrations) in the ponds. Our results demonstrate the complex dynamics and environmental risk of dissolved nutrients in subtropical shrimp ponds, and call for a more effective management of nutrient-laden wastewater in safeguarding the long-term sustainability of aquaculture production
Chromatographic determination of siphonodin content: A rapid and simple strategy for discriminating between Hemsleya omeiensis and other sources of Xuedan
Purpose: To develop a rapid and simple siphonodin content-based high performance liquid chromatography (HPLC) method to distinguish Hemsleya omeiensis from other sources of xuedan.
Methods: Siphonodin was isolated from Hemsleya omeiensis and identified by x-ray crystallographic analysis. An optimized HPLC method was applied for the determination of siphonodin contents of H. omeiensis, H. dolichocarpa and H. gigantha.
Results: Siphonodin was successfully separated by the optimized HPLC method in < 10 min, and the results of validation showed that the HPLC method was stable and very accurate for the quantification of siphonodin. The mean content of siphonodin in 10 batches of H. omeiensis was 3.78 mg/g, but the compound was not detectable in H. dolichocarpa and H. gigantha using the developed HPLC method.
Conclusion: These results indicate that the developed HPLC method is suitable for distinguishing H. omeiensis from other sources of xuedan
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Large fine‐scale spatiotemporal variations of CH4 diffusive fluxes from shrimp aquaculture ponds affected by organic matter supply and aeration in Southeast China
Mariculture shrimp ponds are important CH4 sources to the atmosphere. However, the spatiotemporal variations of CH4 concentration and flux at fine spatial scales in mariculture ponds are poorly known, particularly in China, worlds largest aquaculture producer. In this study, the plot‐scale spatiotemporal variations of water CH4 concentration and flux, both within and among ponds, were researched in shrimp ponds in Shanyutan wetland, Min River Estuary, Southeast China. The average water CH4 concentration and diffusion flux across the water‐air interface in the shrimp ponds over the shrimp aquaculture period varied from 2.29 ± 0.29 to 50.48 ± 20.91 μM and from 0.09 ± 0.01 to 2.32 ± 0.95 mmol·m−2·hr−1, respectively. The CH4 emissions from the estuarine ponds varied greatly between seasons, with peaks in August and September, which was similar to the trend of water temperature and dissolved oxygen concentrations. There was no remarkable difference in CH4 concentration and flux between shrimp ponds but significantly spatiotemporal differences in CH4 concentration and flux within the ponds. Significantly higher emissions occurred in the feeding zone, accounting for approximately 60% of total CH4 emission flux, while much lower CH4 emissions appeared in aeration zone, contributing 14% to total flux. This study suggests the importance of considering spatiotemporal variation in the whole‐pond estimates of CH4 concentration and flux. In light of such high spatial variation within ponds, improving aeration and feed utilization efficiency would help to mitigate CH4 emissions from mariculture ponds
Y Chromosomes of 40% Chinese Are Descendants of Three Neolithic Super-grandfathers
Demographic change of human populations is one of the central questions for
delving into the past of human beings. To identify major population expansions
related to male lineages, we sequenced 78 East Asian Y chromosomes at 3.9 Mbp
of the non-recombining region (NRY), discovered >4,000 new SNPs, and identified
many new clades. The relative divergence dates can be estimated much more
precisely using molecular clock. We found that all the Paleolithic divergences
were binary; however, three strong star-like Neolithic expansions at ~6 kya
(thousand years ago) (assuming a constant substitution rate of 1e-9/bp/year)
indicates that ~40% of modern Chinese are patrilineal descendants of only three
super-grandfathers at that time. This observation suggests that the main
patrilineal expansion in China occurred in the Neolithic Era and might be
related to the development of agriculture.Comment: 29 pages of article text including 1 article figure, 9 pages of SI
text, and 2 SI figures. 5 SI tables are in a separate ancillary fil
Low-energy Scattering of System and the Resonance-like Structure
In this paper, low-energy scattering of the meson
system is studied within L\"uscher's finite-size formalism using
twisted mass gauge field configurations. With three different pion mass values,
the -wave threshold scattering parameters, namely the scattering length
and the effective range , are extracted in channel. Our
results indicate that, in this particular channel, the interaction between the
two vector charmed mesons is weakly repulsive in nature hence do not support
the possibility of a shallow bound state for the two mesons, at least for the
pion mass values being studied. This study provides some useful information on
the nature of the newly discovered resonance-like structure
observed in various experiments.Comment: 11 pages, 6 figures. arXiv admin note: substantial text overlap with
arXiv:1403.131
Proteome analysis of male accessory gland secretions in oriental fruit flies reveals juvenile hormone-binding protein, suggesting impact on female reproduction
In insects, the accessory gland proteins (Acps) secreted by male accessory glands (MAGs) account for the majority of seminal fluids proteins. Mixed with sperm, they are transferred to the female at mating and so impact reproduction. In this project, we identified 2,927 proteins in the MAG secretions of the oriental fruit fly Bactrocera dorsalis, an important agricultural pest worldwide, using LC-MS analysis, and all sequences containing open reading frames were analyzed using signalP. In total, 90Acps were identified. About one third (26) of these 90 Acps had a specific functional description, while the other two thirds (64) had no functional description including dozens of new classes of proteins. Hence, several of these novel Acps were abundant in the MAG secretions, and we confirmed their MAG-specific expression by qPCR. Finally and interestingly, one of these novel proteins was functionally predicted as juvenile hormone-binding protein, suggesting the impact of Acps with reproductive events in the female. Our results will aid in the development of an experimental method to identify Acps in insects, and in turn this information with new Acps in B. dorsalis will pave the way of further exploration their function in reproduction and potential development as new insecticide targets
Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from 2010 to 2011
Porcine epidemic diarrhea has re-emerged with devastating impact in central China since October 2010. To investigate and analyze the reason of this outbreak, the M and ORF3 genes of 15 porcine epidemic diarrhea viruses (PEDV), which were collected from different areas of central China during October 2010 and December 2011, were amplified by reverse transcriptase polymerase chain reaction, cloned, sequenced, and analyzed. Sequence analyses showed that the nucleotides and amino acids were changed at some sites in the M and ORF3 genes of the 15 PEDV strains compared with those genes of CV777 reference strain. Based on the phylogenetic analyses, PEDVs in central China and reference strains could be separated into three groups: G1, G2, and G3. The 15 PEDV strains belonged to G3 group and showed a close relationship with Korean strains (2007), Thai strains (2007–2008), and partial other Chinese strains (2010–2011), but differed genetically from European strains (Br1/87) and the vaccine strain (CV777 vs) being used in China. Furthermore, all 15 PEDV strains from central China and some other isolates in China from 2003 to 2007 (LJB-03, QH, and LZC) belonged to different group. Therefore, PEDV exhibits rapid variation and genetic evolution, and the currently prevailing PEDV strains in central China are a new genotype
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Selection and environmental adaptation along a path to speciation in the Tibetan frog Nanorana parkeri.
Tibetan frogs, Nanorana parkeri, are differentiated genetically but not morphologically along geographical and elevational gradients in a challenging environment, presenting a unique opportunity to investigate processes leading to speciation. Analyses of whole genomes of 63 frogs reveal population structuring and historical demography, characterized by highly restricted gene flow in a narrow geographic zone lying between matrilines West (W) and East (E). A population found only along a single tributary of the Yalu Zangbu River has the mitogenome only of E, whereas nuclear genes of W comprise 89-95% of the nuclear genome. Selection accounts for 579 broadly scattered, highly divergent regions (HDRs) of the genome, which involve 365 genes. These genes fall into 51 gene ontology (GO) functional classes, 14 of which are likely to be important in driving reproductive isolation. GO enrichment analyses of E reveal many overrepresented functional categories associated with adaptation to high elevations, including blood circulation, response to hypoxia, and UV radiation. Four genes, including DNAJC8 in the brain, TNNC1 and ADORA1 in the heart, and LAMB3 in the lung, differ in levels of expression between low- and high-elevation populations. High-altitude adaptation plays an important role in maintaining and driving continuing divergence and reproductive isolation. Use of total genomes enabled recognition of selection and adaptation in and between populations, as well as documentation of evolution along a stepped cline toward speciation
catena-Poly[[tetraaquazinc(II)]-μ-1,3,4-thiadiazol-2,5-diyldithiodiacetato-κ2 O:O′]
In the title linear coordination polymer, [Zn(C6H4N2O4S3)(H2O)4]n, the ZnII atom is coordinated by four O atoms from four water molecules and two O atoms from two [5-(carboxylatomethylsulfanyl)-1,3,4-thiadiazol-2-ylsulfanyl]acetate units in an octahedral coordination environment. The chains are linked into a three-dimensional supramolecular network via O—H⋯O and O—H⋯N hydrogen bonds
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