Dichrostachys cinerea (L.) Wight et Arn (Mimosaceae) hydro-alcoholic extract action on the contractility of tracheal smooth muscle isolated from guinea-pig

<p>Abstract</p> <p>Background</p> <p><it>Dichrostachys cinerea </it>(L.) Wight et Arn. (Mimosaceae) is largely used in ethno-medically across Africa, and mainly employed for the treatment of asthma in Ivory Coast and Gabon. The paper analyses the relaxation induced by the methanolic extract of <it>D. cinerea </it>(Edici) in the guinea-pig trachea preparations (GPTPs). Purpose: This study aimed to bring out the scientific basis to the use of this plant leading to the validation of this phytomedicine.</p> <p>Method</p> <p>The aorta obtained from guinea-pigs was immediately placed in a Mac Ewen solution. Experiments were performed in preparations suspended between two L-shaped stainless steel hooks in a 10 ml organ bath containing Mac Ewen solution. The isometric contractile force of the aorta strips of guinea-pig were recorded by using a strain gauge. The different drugs were directly administered into the organ bath and the magnitude of GPTPs was evaluated.</p> <p>Results</p> <p>Phytochemical analysis of the methanolic extract of Dichrostachys <it>cinerea </it>(Edici) using chemical methods revealed the presence of flavenoids, tannins, sterols, triterpenes and polyphenols. Pharmacological studies performed in GPTPs show that of <it>Dichrostachys cinerea </it>(0.1 mg/ml - 2 mg/ml) evoked a broncho-constriction in GPTPs. Whereas, at concentration up to 2 mg/ml, Edici induced a significant dose-dependent relaxation in the GPTPs. KCl-, ACh- or histamine-evoked contractions of isolated trachea was significantly inhibited by increasing concentrations of Edici (3.5-10 mg/ml). Edici (10 mg/ml) as well as promethazine (0.25 mg/ml) significantly inhibited contractions induced by increasing concentrations of histamine (1×10^-7-1×10^-4mg/ml). In the presence of atropine at a concentration of 10^-6mg/ml, contractile response curve (CRC) evoked by ACh (1×10^-5-1×10^-2mg/ml) was significantly abolished in concentration-dependent manner. Edici did not significantly reduced ACh evoked contraction (10^-5-10^-2mg/ml).</p> <p>Conclusion</p> <p>These observations suggest that Edici could act through two mechanisms: firstly by activation of β-adrenergic or histaminergic receptors; and secondly muscarinic receptors may not be greatly involved, that justifying the use of the extract in traditional Medicine in Africa.</p

Conflation Confers Concurrency

Session types provide a static guarantee that concurrent programs respect communication protocols. Recent work has explored a correspondence between proof rules and cut reduction in linear logic and typing and evaluation of process calculi. This paper considers two approaches to extend logically-founded process calculi. First, we consider extensions of the process calculus to more closely resemble π-calculus. Second, inspired by denotational models of process calculi, we consider conflating dual types. Most interestingly, we observe that these approaches coincide: conflating the multiplicatives (⊗ and 
⅋) allows processes to share multiple channels; conflating the additives (⊕ and &) provides nondeterminism; and conflating the exponentials (! and ?) yields access points, a rendezvous mechanism for initiating session typed communication. Access points are particularly expressive: for example, they are sufficient to encode concurrent state and general recursion

Positive and negative well-being and objectively measured sedentary behaviour in older adults: evidence from three cohorts

Background: Sedentary behaviour is related to poorer health independently of time spent in moderate to vigorous physical activity. The aim of this study was to investigate whether wellbeing or symptoms of anxiety or depression predict sedentary behaviour in older adults. Method: Participants were drawn from the Lothian Birth Cohort 1936 (LBC1936) (n = 271), and the West of Scotland Twenty-07 1950s (n = 309) and 1930s (n = 118) cohorts. Sedentary outcomes, sedentary time, and number of sit-to-stand transitions, were measured with a three-dimensional accelerometer (activPAL activity monitor) worn for 7 days. In the Twenty-07 cohorts, symptoms of anxiety and depression were assessed in 2008 and sedentary outcomes were assessed ~ 8 years later in 2015 and 2016. In the LBC1936 cohort, wellbeing and symptoms of anxiety and depression were assessed concurrently with sedentary behaviour in 2015 and 2016. We tested for an association between wellbeing, anxiety or depression and the sedentary outcomes using multivariate regression analysis. Results: We observed no association between wellbeing or symptoms of anxiety and the sedentary outcomes. Symptoms of depression were positively associated with sedentary time in the LBC1936 and Twenty-07 1950s cohort, and negatively associated with number of sit-to-stand transitions in the LBC1936. Meta-analytic estimates of the association between depressive symptoms and sedentary time or number of sit-to-stand transitions, adjusted for age, sex, BMI, long-standing illness, and education, were β = 0.11 (95% CI = 0.03, 0.18) and β = − 0.11 (95% CI = − 0.19, −0.03) respectively. Conclusion: Our findings indicate that depressive symptoms are positively associated with sedentary behavior. Future studies should investigate the causal direction of this association

Investigation of the presence of human or bovine respiratory syncytial virus in the lungs of mink (Neovison vison) with hemorrhagic pneumonia due to Pseudomonas aeruginosa

<p>Abstract</p> <p>Background</p> <p>Hemorrhagic pneumonia is a disease of farmed mink (<it>Neovison vison</it>) caused by <it>Pseudomonas aeruginosa</it>. The disease is highly seasonal in Danish mink with outbreaks occurring almost exclusively in the autumn. Human respiratory syncytial virus (RSV) has been shown to augment infection with <it>P. aeruginosa</it> in mice and to promote adhesion of <it>P. aeruginosa</it> to human respiratory cells.</p> <p>Findings</p> <p>We tested 50 lung specimens from mink with hemorrhagic pneumonia for bovine RSV by reverse transcriptase polymerase chain reaction (PCR) and for human RSV by a commercial real-time PCR. RSV was not found.</p> <p>Conclusions</p> <p>This study indicates that human and bovine RSV is not a major co-factor for development of hemorrhagic pneumonia in Danish mink.</p

Self Protection from Anti-Viral Responses – Ro52 Promotes Degradation of the Transcription Factor IRF7 Downstream of the Viral Toll-Like Receptors

Ro52 is a member of the TRIM family of single-protein E3 ligases and is also a target for autoantibody production in systemic lupus erythematosus and Sjögren's syndrome. We previously demonstrated a novel function of Ro52 in the ubiquitination and proteasomal degradation of IRF3 following TLR3/4 stimulation. We now present evidence that Ro52 has a similar role in regulating the stability and activity of IRF7. Endogenous immunoprecipitation of Ro52-bound proteins revealed that IRF7 associates with Ro52, an effect which increases following TLR7 and TLR9 stimulation, suggesting that Ro52 interacts with IRF7 post-pathogen recognition. Furthermore, we show that Ro52 ubiquitinates IRF7 in a dose-dependent manner, resulting in a decrease in total IRF7 expression and a subsequent decrease in IFN-α production. IRF7 stability was increased in bone marrow-derived macrophages from Ro52-deficient mice stimulated with imiquimod or CpG-B, consistent with a role for Ro52 in the negative regulation of IRF7 signalling. Taken together, these results suggest that Ro52-mediated ubiquitination promotes the degradation of IRF7 following TLR7 and TLR9 stimulation. As Ro52 is known to be IFN-inducible, this system constitutes a negative-feedback loop that acts to protect the host from the prolonged activation of the immune response

No association for Chinese HBV-related hepatocellular carcinoma susceptibility SNP in other East Asian populations.

published_or_final_versio

Mitotic Spindle Orients Perpendicular to the Forces Imposed by Dynamic Shear

Orientation of the division axis can determine cell fate in the presence of morphogenetic gradients. Understanding how mitotic cells integrate directional cues is therefore an important question in embryogenesis. Here, we investigate the effect of dynamic shear forces on confined mitotic cells. We found that human epithelial cells (hTERT-RPE1) as well as MC3T3 osteoblasts align their mitotic spindle perpendicular to the external force. Spindle orientation appears to be a consequence of cell elongation along the zero-force direction in response to the dynamic shear. This process is a nonlinear response to the strain amplitude, requires actomyosin activity and correlates with redistribution of myosin II. Mechanosteered cells divide normally, suggesting that this mechanism is compatible with biological functions

Chronic obstructive pulmonary disease and inhaled steroids alter surfactant protein D (SP-D) levels: a cross-sectional study

<p>Abstract</p> <p>Background</p> <p>Surfactant protein D (SP-D), an innate immune molecule, plays an important protective role during airway inflammation. Deficiency of this molecule induces emphysematous changes in murine lungs, but its significance in human COPD remains unclear.</p> <p>Methods</p> <p>We collected bronchoalveolar lavage fluid from 20 subjects with varying degrees of COPD (8 former smokers and 12 current smokers) and 15 asymptomatic healthy control subjects (5 never smokers, 3 remote former smokers, and 7 current smokers). All subjects underwent a complete medical history and pulmonary function testing. SP-D was measured by Enzyme-Linked ImmunoSorbent Assay. Statistical analysis was performed using nonparametric methods and multivariable linear regression for control of confounding. The effect of corticosteroid treatment on SP-D synthesis was studied <it>in vitro </it>using an established model of isolated type II alveolar epithelial cell culture.</p> <p>Results</p> <p>Among former smokers, those with COPD had significantly lower SP-D levels than healthy subjects (median 502 and 1067 ng/mL, respectively, p = 0.01). In a multivariable linear regression model controlling for age, sex, race, and pack-years of tobacco, COPD was independently associated with lower SP-D levels (model coefficient -539, p = 0.04) and inhaled corticosteroid use was independently associated with higher SP-D levels (398, p = 0.046). To support the hypothesis that corticosteroids increase SP-D production we used type II alveolar epithelial cells isolated from adult rat lungs. These cells responded to dexamethasone treatment by a significant increase of SP-D mRNA (p = 0.041) and protein (p = 0.037) production after 4 days of culture.</p> <p>Conclusion</p> <p>Among former smokers, COPD is associated with lower levels of SP-D and inhaled corticosteroid use is associated with higher levels of SP-D in the lung. Dexamethasone induced SP-D mRNA and protein expression in isolated epithelial cells <it>in vitro</it>. Given the importance of this molecule as a modulator of innate immunity and inflammation in the lung, low levels may play a role in the pathogenesis and/or progression of COPD. Further, we speculate that inhaled steroids may induce SP-D expression and that this mechanism may contribute to their beneficial effects in COPD. Larger, prospective studies are warranted to further elucidate the role of surfactant protein D in modulating pulmonary inflammation and COPD pathogenesis.</p

How a slow-ovipositing parasitoid can succed as a biological control agent of the invasive mealybug Phenacoccus peruvianus: implications for future classical and conservation biological control programs

[EN] Phenaccocus peruvianus Granara de Willink (Hemiptera: pseudococcidae) is an invasive mealybug that has become a pest of ornamental plants in Europe and has recently been detected in California, USA. In this work, we studied the tritrophic interaction among this mealybug, its main parasitoid Acerophagus n. sp. near coccois (Hymenoptera: Encyrtidae) and tending ants to disclose the success of this parasitoid controlling P. peruvianus. Acerophagus n. sp. near coccois accepted mealybugs for parasitism regardless of their size but did not hostfeed. We recorded three active defenses of P. peruvianus. Host handling time-consuming process that required more than 30 min. Tending ants, Lasius grandis (Hymenoptera: Encyrtidae), reduced the time spent by parasitoids in a patch and disrupted oviposition attempts. The low numbers of ants tending mealybugs colonies in Spain and France could explain why this parasitoid, with a long handling time, is an efficient biological control agent for P. peruvianus.Beltrà Ivars, A.; Soto Sánchez, AI.; Tena Barreda, A. (2015). How a slow-ovipositing parasitoid can succed as a biological control agent of the invasive mealybug Phenacoccus peruvianus: implications for future classical and conservation biological control programs. BioControl. 60(4):473-484. https://doi.org/10.1007/s10526-015-9663-6S473484604Arakelian G (2013) Bougainvillea mealybug (Phenacoccus peruvianus). Factsheet 2013. County of Los Angeles. Department of agricultural commissioner/weights and measures, USABartlett BR (1961) The influence of ants upon parasites, predators, and scale insects. Ann Entomol Soc Am 54:543–551Bartlett BR (1978) Pseudococcidae. In: Clausen CP (ed) Introduced parasites and predators of arthropod pests and weeds: a world review, 1st edn. Agricultural Research Service USDA, Washington, USA, pp 137–170Barzman MS, Daane KM (2001) Host-handling behaviors in parasitoids of black scale, Saissetia oleae (Homoptera: Coccidae): a case for ant-mediated evolution. J Anim Ecol 70:237–247Beltrà A, Soto A, Germain JF, Matile-Ferrero D, Mazzeo G, Pellizzari G, Russo A, Franco JC, Williams DJ (2010) The Bougainvillea mealybug Phenacoccus peruvianus, a rapid invader from South America to Europe. Entomol Hell 19:137–143Beltrà A, Garcia-Marí F, Soto A (2013a) Seasonal phenology, spatial distribution, and sampling plan for the invasive mealybug Phenacoccus peruvianus (Hemiptera: Pseudococcidae). J Econ Entomol 106:1486–1494Beltrà A, Tena A, Soto A (2013b) Fortuitous biological control of the invasive mealybug Phenacoccus peruvianus in Southern Europe. BioControl 58:309–317Beltrà A, Tena A, Soto A (2013c) Reproductive strategies and food sources used by Acerophagus n. sp. near coccois, a new successful parasitoid of the invasive mealybug Phenacoccus peruvianus. J Pest Sci 86:253–259Berlinger MJ, Golberg AM (1978) The effect of the fruit sepals on the citrus mealybug population and on its parasite. Entomol Exp Appl 24:238–243Blumstein DT, Daniel JC (2007) Quantifying behavior the JWatcher way. Sinauer Associates Inc., Sunderland, UKBoavida C, Ahounou M, Vos M, Neuenschwander P, van Alphen JJM (1995) Host stage selection and sex allocation by Gyranusoidea tebygi (Hymenoptera: Encyrtidae), a parasitoid of the mango mealybug, Rastrococcus invadens (Homoptera: Pseudococcidae). Biol Control 5:487–496Bokonon-Ganta AH, Neuenschwander P, van Alphen JJM, Vos M (1995) Host stage selection and sex allocation by Anagyrus mangicola (Hymenoptera: Encyrtidae), a parasitoid of the mango mealybug, Rastrococcus invadens (Homoptera: Pseudococcidae). Biol Control 5:479–486Bugila AAA, Franco JC, Borges da Silva E, Branco M (2014a) Defense response of native and alien mealybugs (Hemiptera: Pseudococcidae) against the solitary parasitoid Anagyrus sp. nr. pseudococci (Girault) (Hymenoptera: Encyrtidae). J Insect Behav 27:439–453Bugila AAA, Branco M, Borges da Silva E, Franco JC (2014b) Host selection behavior and specificity of the solitary parasitoid of mealybugs Anagyrus sp. nr. pseudococci (Girault) (Hymenoptera, Encyrtidae). Biocontrol Sci Techn 24:22–38Bynum EK (1937) Pseudococcobius terryi Fullaway, a Hawaiian parasite of Gray Sugarcane mealybug in the United States. J Econ Entomol 30:756–761Cadée N, van Alphen JJM (1997) Host selection and sex allocation in Leptomastidea abnormis, a parasitoid of the citrus mealybug Planococcus citri. Entomol Exp Appl 83:277–284Clausen CP (1924) The parasites of Pseudococcus maritimus (Ehrhorn) in California (Hymenoptera, Chalcidoidea). Part II. Biological studies and life histories. UC Pub Entomol 3:253–288Daane KM, Barzman MS, Caltagirone LE, Hagen KS (2000) Metaphycus anneckei and Metaphycus hageni: two discrete species parasitic on black scale, Saissetia oleae. BioControl 45:269–284Daane KM, Bentley WJ, Walton VM, Malakar-Kuenen R, Millar JC, Ingels CA, Weber EA, Gispert C (2006) New controls investigated for vine mealybug. Calif Agric 60:31–38Daane KM, Sime KR, Fallon J, Cooper ML (2007) Impacts of Argentine ants on mealybugs and their natural enemies in California’s coastal vineyards. Ecol Entomol 32:583–596De Farias AM, Hopper KR (1999) Oviposition behavior of Aphelinus asychis (Hymenoptera: Aphelinidae) and Aphidius matricariae (Hymenoptera: Aphidiidae) and defense behavior of their host Diuraphis noxia (Homoptera: Aphididae). Environ Entomol 28:858–862Dorn B, Mattiacci L, Bellotti AC, Dorn S (2001) Host specificity and comparative foraging behavior of Aenasius vexans and Acerophagus coccois, two endo-parasitoids of the cassava mealybug. Entomol Exp Appl 99:331–339Eisner T, Silberglied RE (1988) A chrysopid larva that cloaks itself in mealybug wax. Psyche 95:15–20Flanders SE (1963) Predation by parasitic Hymenoptera, the basis of ant-induced outbreaks of a host species. J Econ Entomol 56:116Foldi I (1983) Structure et fonctions des glandes tégumentaires de cochenilles Pseudococcines et de leurs secretions. Ann Soc Entomol Fr 19:155–156Foldi I (1997) Defense strategies in scale insects: phylogenetic inference and evolutionary scenarios (Hemiptera, Coccoidea). In: Grandcolas P (ed) The origin of biodiversity in insects: phylogenetic tests of evolutionary scenarios, 1st edn. Muséum National d’Histoire Naturelle, Paris, France, pp 203–230Godfray HCJ (1994) Parasitoids: behavioral and evolutionary ecology. Princeton University Press, Princeton, USAGonzález-Hernández H, Johnson MW, Reimer NJ (1999) Impact of Pheidole megacephala (F.) (Hymenoptera: Formicidae) on the biological control of Dysmicoccus brevipes (Cockerell) (Homoptera: Pseudococcidae). Biol Control 15:145–152Gross P (1993) Insect behavioral and morphological defenses against parasitoids. Annu Rev Entomol 38:251–273Gullan PJ (1997) Relationships with ants. In: Ben-Dov Y, Hodgson CJ (eds) Soft scale insects—their biology natural enemies and control, 1st edn. Elsevier, Amsterdam, The Netherlands, pp 351–373Gullan PJ, Kosztarab M (1997) Adaptations in scale insects. Annu Rev Entomol 42:23–50Hcidari M, Jahan M (2000) A study of ovipositional behavior of Anagyrus pseudococci a parasitoid of mealybugs. J Agric Sci Technol 2:49–53Honda JY, Luck RF (1995) Scale morphology effects on feeding behavior and biological control potential of Rhyzobius lophanthae (Coleoptera: Coccinellidae). Ann Entomol Soc Am 88:441–450Joyce AL, Hoddle MS, Bellows TS, Gonzalez D (2001) Oviposition behavior of Coccidoxenoides peregrinus, a parasitoid of Planococcus ficus. Entomol Exp Appl 98:49–57Karamaouna F (1999) Biology of the parasitoids Leptomastix epona (Walker) and Pseudaphycus flavidulus (Brèthes) and behavioural interactions with the host mealybug Pseudococcus viburni (Signoret). Ph.D. Thesis, University of London, UK, p 333Karamaouna F, Copland MJ (2000) Oviposition behavior, influence of experience on host size selection, and niche overlap of the solitary Leptomastix epona and the gregarious Pseudaphycus flavidulus, two endoparasitoids of the mealybug Pseudococcus viburni. Entomol Exp Appl 97:301–308Klotz JH, Hansen L, Pospischil R, Rust M (2008) Urban ants of North America and Europe. Cornell University Press, Ithaca, USAMailleux AC, Deneubourg JL, Detrain C (2003) Regulation of ants foraging to resource productivity. P R Soc Lond B Bio 270:1609–1616Majerus ME, Sloggett JJ, Godeau JF, Hemptinne JL (2007) Interactions between ants and aphidophagous and coccidophagous ladybirds. Popul Ecol 49:15–27Mgocheki N, Addison P (2009) Interference of ants (Hymenoptera: Formicidae) with biological control of the vine mealybug Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae). Biol Control 49:180–185Moore D (1988) Agents used for biological control of mealybugs (Pseudococcidae). Biocontrol News Inf 9:209–225Paris CI, Espadaler X (2009) Honeydew collection by the invasive garden ant Lasius neglectus versus the native ant L grandis. Arthropod Plant Interact 3:75–85Pekas A, Tena A, Aguilar A, Garcia-Marí F (2011) Spatio-temporal patterns and interactions with honeydew-producing Hemiptera of ants in a Mediterranean citrus orchard. Agric Forest Entomol 13:89–97Pennacchio F, Strand MR (2006) Evolution of developmental strategies in parasitic Hymenoptera. Annu Rev Entomol 51:233–258Pijls JW, Hofker KD, Staalduinen MJ, van Alphen JJM (1995) Interspecific host discrimination and competition in Apoanagyrus (Epidinocarsis) lopezi and A(E) diversicornis parasitoids of the cassava mealybug Phenacoccus manihoti. Ecol Entomol 20:326–332Robert Y (1987) Dispersion and migration. In: Minks AK, Harrewijn P (eds) Aphids—their biology, natural enemies and control, 1st edn. Elsevier, Amsterdam, The Netherlands, pp 299–313Sandanayaka WRM, Charles JG, Allan DJ (2009) Aspects of the reproductive biology of Pseudaphycus maculipennis (Hym: Encyrtidae), a parasitoid of obscure mealybug, Pseudococcus viburni (Hem: Pseudococcidae). Biol Control 48:30–35Sarkar D (2008) Lattice: multivariate data visualization with R. Springer, New York, USASime KR, Daane KM (2014) Rapid, non-discriminatory oviposition behaviors are favored in mealybug parasitoids when Argentine ants are present. Environ Entomol 43:995–1002Tena A, Garcia-Marí F (2008) Suitability of citricola scale Coccus pseudomagnoliarum (Hemiptera: Coccidae) as host of Metaphycus helvolus (Hymenoptera: Encyrtidae): Influence of host size and encapsulation. Biol Control 46:341–347Tena A, Hoddle CD, Hoddle MS (2013) Competition between honeydew producers in an ant–hemipteran interaction may enhance biological control of an invasive pest. Bull Entomol Res 103:714–723The R Core Team (2011) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austriavan Driesche RG, Belloti A, Herrera CJ, Castello JA (1987a) Host preferences of two encyrtid parasitoids for the Columbian Phenacoccus spp. of cassava mealybugs. Entomol Exp Appl 43:261–266van Driesche RG, Belloti A, Herrera CJ, Castello JA (1987b) Host feeding and ovipositor insertion as sources of mortality in the mealybug Phenacoccus herreni caused by two encyrtids Epidinocarsis diversicornis and Acerophagus coccois. Entomol Exp Appl 44:97–100Vet LE, Dicke M (1992) Ecology of infochemical use by natural enemies in a tritrophic context. Annu Rev Entomol 37:141–172Völkl W, Woodring J, Fischer M, Lorenz MW, Hoffmann KH (1999) Ant-aphid mutualisms: the impact of honeydew production and honeydew sugar composition on ant preferences. Oecologia 118:483–491Wajnberg E (1989) Analysis of variations of handling-time in Trichogramma maidis. Entomophaga 34:397–407Way MJ (1963) Mutualism between ants and honeydew-producing Homoptera. Annu Rev Entomol 8:307–344Weiss MR (2006) Defecation behavior and ecology of insects. Annu Rev Entomol 51:635–661Wyckhuys KAG, Stone L, Desneux N, Hoelmer KA, Hopper KR, Heimpel GE (2008) Parasitism of the soybean aphid Aphis glycines by Binodoxys communis: the role of aphid defensive behavior and parasitoid reproductive performance. Bull Entomol Res 98:361–370Zain-ul-Abdin, Arif MJ, Suhail A, Gogi MD, Arshad M, Wakil W, Abbas SK, Altaf A, Shaina H, Manzoor A (2012) Molecular analysis of the venom of mealybug parasitoid Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae). Pak Entomol 34:189–193Zinna G (1959) Specializzazione entomoparassitica negli Encyrtidae: studio morfologico etologico e fisiologico del Leptomastix dactylopii. Howard Boll Lab agr Filippo Silvestri 18:1–14