Utrensing av små korn kan redusere innholdet av mykotoksiner i havrepartir

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Faces of a Changing Climate: Semi-Quantitative Multi-Mycotoxin Analysis of Grain Grown in Exceptional Climatic Conditions in Norway

Recent climatological research predicts a significantly wetter climate in Southern Norway as a result of global warming. Thus, the country has already experienced unusually wet summer seasons in the last three years (2010–2012). The aim of this pilot study was to apply an existing multi-analyte LC-MS/MS method for the semi-quantitative determination of 320 fungal and bacterial metabolites in Norwegian cereal grain samples from the 2011 growing season. Such knowledge could provide important information for future survey and research programmes in Norway. The method includes all regulated and well-known mycotoxins such as aflatoxins, trichothecenes, ochratoxin A, fumonisins and zearalenone. In addition, a wide range of less studied compounds are included in the method, e.g., Alternaria toxins, ergot alkaloids and other metabolites produced by fungal species within Fusarium, Penicillium and Aspergillus. Altogether, 46 metabolites, all of fungal origin, were detected in the 76 barley, oats and wheat samples. The analyses confirmed the high prevalence and relatively high concentrations of type-A and -B trichothecenes (e.g., deoxynivalenol up to 7230 µg/kg, HT-2 toxin up to 333 µg/kg). Zearalenone was also among the major mycotoxins detected (maximum concentration 1670 µg/kg). Notably, several other Fusarium metabolites such as culmorin, 2-amino-14,16-dimethyloctadecan-3-ol and avenacein Y were co-occurring. Furthermore, the most prevalent Alternaria toxin was alternariol with a maximum concentration of 449 µg/kg. A number of Penicillium and Aspergillus metabolites were also detected in the samples, e.g., sterigmatocystin in concentrations up to 20 µg/kg

Pathogenicity, host specificity and genetic diversity in Norwegian isolates of Microdochium nivale and Microdochium majus

Microdochium majus and Microdochium nivale cause serious disease problems in grasses and cereal crops in the temperate regions. Both fungi can infect the plants during winter (causing pink snow mould) as well as under cool humid conditions during spring and fall. We conducted a pathogenicity test of 15 M. nivale isolates and two M. majus isolates from Norway at low temperature on four different grass cultivars of Lolium perenne and Festulolium hybrids. Significant differences between M. nivale isolates in the ability to cause pink snow mould were detected. The M. nivale strains originally isolated from grasses were more pathogenic than isolates from cereals. The genetic diversity of M. nivale and M. majus isolates was studied by sequencing four genetic regions; Elongation factor-1 alpha (EF-1α), β-tubulin, RNA polymerase II (RPB2) and the Internal Transcribed Spacer (ITS). Phylogenetic trees based on the sequences of these four genetic regions resolved M. nivale and M. majus isolates into separate clades. Higher genetic diversity was found among M. nivale isolates than among M. majus isolates. M. nivale isolates revealed genetic differences related to different host plants (grasses vs. cereals) and different geographic regions (Norway and UK vs. North America). Sequence results from the RPB2 and β-tubulin genes were more informative than those from ITS and EF-1α. The genetic and phenotypic differences detected between Norwegian M. nivale isolates from cereals and grasses support the assumption that host specialization exist within M. nivale isolates.publishedVersio

Effekt av redusert jordarbeiding på halmdekke, avling, ugras, Fusarium og mykotoksiner i havre

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Fusarium langsethiae and mycotoxin contamination in oat grain differed with growth stage at inoculation

High levels of mycotoxins are occasionally observed in Norwegian oat grain lots. Mycotoxins of primary concern in Norwegian oats are deoxynivalenol (DON) produced by Fusarium graminearum and HT2- and T2-toxins (HT2 + T2) produced by Fusarium langsethiae. Improved understanding of the epidemiology of Fusarium spp. is important for the development of measures to control mycotoxins. We studied the susceptibility to F. langsethiae after inoculation at early (booting, heading, flowering) or late (flowering, milk, dough) growth stages in three oat varieties in greenhouse experiments. The varieties had previously shown different levels of resistance to F. graminearum: Odal, Vinger (both moderately resistant), and Belinda (susceptible). The level of F. langsethiae DNA and HT2 + T2 were measured in harvested grain. In addition, we observed differences in aggressiveness (measured as the level of F. langsethiae DNA in grain) between F. langsethiae isolates after inoculation of oats at flowering. Substantial levels of F. langsethiae DNA (mean ≥ 138 pg per μg plant DNA) and HT2 + T2 (≥348 μg/kg) were detected in grain harvested from oats that were spray-inoculated at heading or later stages, but not at booting (mean ≤ 10 pg/μg and ≤ 25 μg/kg, respectively), suggesting that oats are susceptible to F. langsethiae from heading and onwards. Vinger was the most resistant variety to F. langsethiae/HT2 + T2, whereas Odal and Belinda were relatively susceptible. We observed that late inoculations yielded high levels of other trichothecene A metabolites (mean sum of metabolites of 35–1048 μg/kg) in addition to HT2 + T2, in harvested grain, an indication that infections close to harvest may pose a further risk to food and feed safety.publishedVersio

Global transcriptome changes in perennial ryegrass during early infection by pink snow mould

Lack of resistance to pink snow mould (Microdochium nivale) is a major constraint for adaptation of perennial ryegrass (Lolium perenne L.) to continental regions with long-lasting snow cover at higher latitudes. Almost all investigations of genetic variation in resistance have been performed using cold acclimated plants. However, there may be variation in resistance mechanisms that are functioning independently of cold acclimation. In this study our aim was to identify candidate genes involved in such resistance mechanisms. We first characterized variation in resistance to M. nivale among non-acclimated genotypes from the Norwegian cultivar ‘Fagerlin’ based on relative regrowth and fungal quantification by real-time qPCR. One resistant and one susceptible genotype were selected for transcriptome analysis using paired-end sequencing by Illumina Hiseq 2000. Transcriptome profiles, GO enrichment and KEGG pathway analysis indicate that defense response related genes are differentially expressed between the resistant and the susceptible genotype. A significant up-regulation of defense related genes, as well as genes involved in cell wall cellulose metabolic processes and aryl-alcohol dehydrogenase (NADP+) activity, was observed in the resistant genotype. The candidate genes identified in this study might be potential molecular marker resources for breeding perennial ryegrass cultivars with improved resistance to pink snow mould.publishedVersio

Effekter av ulik jordarbeiding i korn

Hvilken jordarbeiding som benyttes i den enkelte kornåker påvirker blant annet avlingsmengde, kvaliteten på kornet og miljøet. I denne publikasjonen er det samlet informasjon om effekter av ulik jordarbeiding, som hjelp til korndyrkere ved vurdering av jordarbeidingsmetoder, og for myndigheter ved beslutninger om jordarbeiding i regionale miljøprogram (RMP)

Effekter av ulik jordarbeiding i korn

Hvilken jordarbeiding som benyttes i den enkelte kornåker påvirker blant annet avlingsmengde, kvaliteten på kornet og miljøet. I denne publikasjonen er det samlet informasjon om effekter av ulik jordarbeiding, som hjelp til korndyrkere ved vurdering av jordarbeidingsmetoder, og for myndigheter ved beslutninger om jordarbeiding i regionale miljøprogram (RMP)

Muligheter for en mer effektiv utnytelse av planterestene - Agronomi som sikrer god jordhelse, avling og plantehelse i korn

Tap av organisk materiale, jordpakking og erosjon truer jordhelsa på kornareal. Problemer med dette vil antagelig øke i et våtere klima og medføre store kostnader for både gårdbrukere og samfunn. Fremover må vi passe på å stabilisere erosjonsutsatt jordoverflate og sikre en god infiltrasjon av nedbør. På kornareal er lav årlig tilførsel av karbon en begrensende faktor for aggregering og stabilisering, men dette kan forbedres ved å beholde halmen på jordet eller bruke en tilpasset fangvekststrategi. En bør trolig skjevfordele tilført organisk materiale mer mot jordas overflate og dermed stimulere mikrobiell aktivitet i jordas toppsjikt. Da må en minimere jordarbeidingsintensiteten. Slik redusert jordarbeiding fører også til utvikling av et kontinuerlig poresystem nedover i profilet som kan øke infiltrasjonen etter kraftige nedbørsepisoder og dermed bidra til å dempe flomtopper. Store mengder plantemateriale ved jordoverflaten gir imidlertid også noen utfordringer. Det trengs økt kunnskap om ugrasbekjempelse, spesielt i et scenario der glyfosat blir forbudt. Minimal jordarbeiding med planterester på jordoverflaten kan også øke angrep av sopp. Integrerte plantevernstrategier bør identifisere arter og sorter av matplanter og fangvekster som kan bidra til å begrense forekomst av patogener i jord og halmrester. Bedre jordhelse på kornareal er en tverrfaglig utfordring og krever en varig endring av dagens dyrkingspraksis.Muligheter for en mer effektiv utnytelse av planterestene - Agronomi som sikrer god jordhelse, avling og plantehelse i kornpublishedVersio