Toxic Byproduct Formation during Electrochemical Treatment of Latrine Wastewater

Electrochemical systems are an attractive option for onsite latrine wastewater treatment due to their high efficiency and small footprint. While concerns remain over formation of toxic byproducts during treatment, rigorous studies examining byproduct formation are lacking. Experiments treating authentic latrine wastewater over variable treatment times, current densities, chloride concentrations, and anode materials were conducted to characterize byproducts and identify conditions that minimize their formation. Production of inorganic byproducts (chlorate and perchlorate) and indicator organic byproducts (haloacetic acids and trihalomethanes) during electrolysis dramatically exceeded recommendations for drinking water after one treatment cycle (∼10–30 000 times), raising concerns for contamination of downstream water supplies. Stopping the reaction after ammonium was removed (i.e., the chlorination breakpoint) was a promising method to minimize byproduct formation without compromising disinfection and nutrient removal. Though treatment was accelerated at increased chloride concentrations and current densities, byproduct concentrations remained similar near the breakpoint. On TiO_2/IrO_2 anodes, haloacetic acids (up to ∼50 μM) and chlorate (up to ∼2 μM) were of most concern. Although boron-doped diamond anodes mineralized haloacetic acids after formation, high production rates of chlorate and perchlorate (up to ∼4 and 25 μM) made them inferior to TiO_2/IrO_2 anodes in terms of toxic byproduct formation. Organic byproduct formation was similar during chemical chlorination and electrolysis of wastewater, suggesting that organic byproducts are formed by similar pathways in both cases (i.e., reactions with chloramines and free chlorine)

Multilayer Heterojunction Anodes for Saline Wastewater Treatment: Design Strategies and Reactive Species Generation Mechanisms

Multilayer heterojunction SbSn/CoTi/Ir anodes, which consist of Ir_(0.7)Ta_(0.3)O_2 bottom layers coated onto a titanium base, Co-TiO_2 interlayers, and overcoated discrete Sb-SnO_2 islands, were prepared by spray pyrolysis. The Ir_(0.7)Ta_(0.3)O_2 bottom layer serves as an Ohmic contact to facilitate electron transfer from semiconductor layers to the Ti base. The Co-TiO_2 interlayer and overcoated Sb-SnO_2 islands enhance the evolution of reactive chlorine. The surficial Sb-SnO_2 islands also serve as the reactive sites for free radical generation. Experiments coupled with computational kinetic simulations show that while ·OH and Cl· are initially produced on the SbSn/CoTi/Ir anode surface, the dominant radical formed in solution is the dichlorine radical anion, Cl_2·–. The steady-state concentration of reactive radicals is 10 orders of magnitude lower than that of reactive chlorine. The SbSn/CoTi/Ir anode was applied to electrochemically treat human wastewater. These test results show that COD and NH_4^+ can be removed after 2 h of electrolysis with minimal energy consumption (370 kWh/kg COD and 383 kWh/kg NH_4^+). Although free radical species contribute to COD removal, anodes designed to enhance reactive chlorine production are more effective than those designed to enhance free radical production

Seabed pockmarks and subsurface fluid migration at multiple scales: Investigations using hydroacoustic and seismic data

Fluid migration from deep sedimentary basins towards Earth’s surface has various implications for hydrocarbon accumulations and influences slope stability, climate and ecological systems. Fluids seeping from the seafloor can give insight into deep crustal and tectonic processes and can significantly change the seafloor morphology as well as the chemical composition of the overlying ocean. The timing of fluid migration in many sedimentary basins is often poorly constrained and the composition of fluids involved in the formation of migration pathways and seafloor venting structures is difficult to determine. Likewise, the effect of upward migrating fluids on the surrounding host sediments and their diagenetic processes is an under-investigated field. Although gas bubbles venting from the seafloor are well constrained and easily identified in hydroacoustic data, the detection of submarine groundwater discharge sites often relies on oral traditions and visual reports from fishermen who recognize anomalies (schlieren/streaks) on the sea-surface. In many regions (e.g. organic muds, hydrothermal fields), submarine groundwater discharge is accompanied by gas venting. However, the processes involved in simultaneous gas and water discharge, as well as their relative contributions to geomorphological structures, are generally not well understood. Decreasing acoustic resolution with depth requires a multi-scale approach to gain a better understanding of the various fluids and migration processes involved at different depths. Using different hydroacoustic systems and frequencies, I examine fluid migration pathways in the subsurface and various morphological expressions that seeping fluids create on the surface during discharge. I integrate well logs, surface sediment grab samples, as well as sediment cores and geochemical porewater analysis to validate and ground truth the hydroacoustic and seismic observations. I apply these methods to datasets from two study areas: the Canterbury Basin, east of New Zealand’s South Island, and Eckernförde Bay in the Baltic Sea of Northern Germany. In the Canterbury Basin, my analyses reveal a wide variety of subsurface migration pathways as well as surface structures related to fluid migration. I show how diagenetic processes of fine-grained sediments are dramatically changed in a 2 km radial distance around a conduit feeding a sediment volcano. This change manifests itself in the suppression of polygonal faulting, and is a result of either 1) a significant change in differential stress induced by buoyant upward migrating fluids that accumulate at depth, or 2) permeable stringers intruding into the surroundings of the feeding pipe and therefore facilitating the dewatering of the enclosing sediments. On the surface of the Oligocene Marshall Paraconformity, I find pockmarks as well as discharged sediments emplaced by sediment volcanism. While the pockmarks appear to be related to dewatering mechanisms of the underlying strata, the sediments emplaced on the same surface seem to be sourced from Cretaceous strata. Several sediment intrusions into Paleocene sediments are similarly sourced from Cretaceous lithologies and affect the overlying fault orientation. Also, I find that recent fluid migration pathways are likely to be responsible for shallow gas accumulations on the continental slope of the Canterbury Basin. On the present-day seafloor, there are numerous pockmarks on the shelf and slope that have been modified by currents. The pockmarks form as a result of gas and/or groundwater seepage, but the contribution of gas versus offshore groundwater could not be unequivocally determined for the Canterbury Basin. In Eckernförde Bay, in contrast to the Canterbury Basin, I was able to hydroacoustically distinguish areas of submarine groundwater discharge and areas additionally affected by gas seepage. Using very high-resolution multibeam data and sub-bottom profiling, I observed and characterised a new type of pockmark that is associated with submarine groundwater and gas discharge. I determined that, in gaseous muddy sediments, submarine groundwater discharge results in unusually consistent and exceptionally shallow free gas that can even be detected with high-frequency 400 kHz multibeam systems

Multiannual Seafloor Dynamics around a Subtidal Rocky Reef Habitat in the North Sea

Rocky reefs provide complex structures in the otherwise largely sand-dominated coastal North Sea. Therefore, these reefs are highly important natural habitats for the functioning of coastal ecosystems, as they provide shelter, refuge and nursery grounds for various mobile and sessile species. In the North Sea, the spatial distribution of these habitats has been intensively investigated over recent years. However, these studies generally provide static accounts of the current state of these reef systems, but limited data exist on the temporal variations in sediment dynamics at and around natural rocky reefs. In this study, we provide observations from a multiannual time series of hydroacoustic seafloor surveys conducted at an isolated rocky reef in the North Sea. We use multibeam bathymetry and side-scan sonar backscatter data in combination with video observations, sediment sampling, and sub-bottom profiler data to assess the long-term variations of the rocky reef system. The reef is located in water depths between 11 and 17 m with an areal extent of ~0.5 km2 and is surrounded by mobile sands. The topography of the rocky reef appears to create a distinct hydrodynamic system that permits mobile sands to settle or move into bathymetrical deeper parts of the reef. Our results suggest a very dynamic system surrounding the reef with large scale scouring, sediment reworking and transport, while the shallower central part of the reef remains stable over time. We demonstrate the importance of hydrodynamics and current scouring around reefs for the local variability in seafloor properties over time. These small-scale dynamics are likewise reflected in the spatial distribution of sessile species, which are less abundant in proximity to mobile sands. The hydroacoustic mapping and monitoring of seafloor dynamics at higher spatial and temporal resolutions presents an important future direction in the study of valuable coastal habitats

A genomic approach to inferring kinship reveals limited intergenerational dispersal in the yellow fever mosquito

Understanding past dispersal and breeding events can provide insight into ecology and evolution, and can help inform strategies for conservation and the control of pest species. However, parent-offspring dispersal can be difficult to investigate in rare species and in small pest species such as mosquitoes. Here we develop a methodology for estimating parent-offspring dispersal from the spatial distribution of close kin, using pairwise kinship estimates derived from genome-wide single nucleotide polymorphisms (SNPs). SNPs were scored in 162 Aedes aegypti (yellow fever mosquito) collected from eight close-set, high-rise apartment buildings in an area of Malaysia with high dengue incidence. We used the SNPs to reconstruct kinship groups across three orders of kinship. We transformed the geographical distances between all kin pairs within each kinship category into axial standard deviations of these distances, then decomposed these into components representing past dispersal events. From these components, we isolated the axial standard deviation of parent-offspring dispersal, and estimated neighbourhood area (129 m), median parent-offspring dispersal distance (75 m), and oviposition dispersal radius within a gonotrophic cycle (36 m). We also analysed genetic structure using distance-based redundancy analysis and linear regression, finding isolation by distance both within and between buildings and estimating neighbourhood size at 268 individuals. These findings indicate the scale required to suppress local outbreaks of arboviral disease and to target releases of modified mosquitoes for mosquito and disease control. Our methodology is readily implementable for studies of other species, including pests and species of conservation significance

DAM pilot project: Exclusion of bottom trawl fishery in marine protected areas of the German EEZ (North Sea) - DAM MPA Geo 1, Cruise No. HE588, October 24 - November 4, 2021, Bremerhaven (Germany) - Bremerhaven (Germany)

During HE588, data were collected in five research areas in the south-eastern part of the German Bight as part of the DAM Pilotmission on the exclusion of mobile bottom-contact fishing in the North Sea (www.mgf-nordsee.de). The cruise started on October 24, 2021, and had a duration of twelve days at sea. The conducted tasks consisted of seafloor mapping with hydroacoustic devices, multicoring and grab sampling from the seafloor surface, lander deployments for the study of current characteristics, and video and diving surveys of benthic fauna. Despite the unstable weather conditions, all scientific tasks could be conducted successfully within the allocated time

DWNN, a novel ubiquitin-like domain, implicates RBBP6 in mRNA processing and ubiquitin-like pathways

BACKGROUND: RBBP6 is a 250 kDa splicing-associated protein that has been identified as an E3 ligase due to the presence of a RING finger domain. In humans and mice it interacts with both p53 and Rb, and plays a role in the induction of apoptosis and regulation of the cell cycle. RBBP6 has recently been shown to be highly up-regulated in oesophageal cancer, and to be a promising target for immunotherapy against the disease. RESULTS: We show here using heteronuclear NMR that the N-terminal 81 amino acids of RBBP6 constitute a novel ubiquitin-like domain, which we have called the DWNN domain. The domain lacks conserved equivalents of K(48 )and K(63), although the equivalents of K(6 )and K(29 )are highly, although not absolutely, conserved. The di-glycine motif that is characteristic of proteins involved in ubiquitination is found in the human and mouse form of the domain, although it is not present in all organisms. It forms part of a three-domain form of RBBP6 containing the DWNN domain, a zinc knuckle and a RING finger domain, which is found in all eukaryotic genomes so far examined, in the majority of cases at single copy number. The domain is also independently expressed in vertebrates as a single domain protein. CONCLUSION: DWNN is a novel ubiquitin-like domain found only at the N-terminus of the RBBP6 family of splicing-associated proteins. The ubiquitin-like structure of the domain greatly increases the likelihood that RBBP6 functions through some form of ubiquitin-like modification. Furthermore, the fact that the DWNN domain is independently expressed in higher vertebrates leads us to propose that the domain may itself function as a novel ubiquitin-like modifier of other proteins

Enhancing the activity of oxygen-evolution and chlorine-evolution electrocatalysts by atomic layer deposition of TiO₂

We report that TiO₂ coatings formed via atomic layer deposition (ALD) may tune the activity of IrO₂, RuO₂, and FTO for the oxygen-evolution and chlorine-evolution reactions (OER and CER). Electrocatalysts exposed to ∼3–30 ALD cycles of TiO₂ exhibited overpotentials at 10 mA cm⁻² of geometric current density that were several hundred millivolts lower than uncoated catalysts, with correspondingly higher specific activities. For example, the deposition of TiO₂ onto IrO₂ yielded a 9-fold increase in the OER-specific activity in 1.0 M H₂SO₄ (0.1 to 0.9 mA cm_(ECSA)⁻² at 350 mV overpotential). The oxidation state of titanium and the potential of zero charge were also a function of the number of ALD cycles, indicating a correlation between oxidation state, potential of zero charge, and activity of the tuned electrocatalysts

Igneous seismic geomorphology of buried lava fields and coastal escarpments on the Vøring volcanic rifted margin

Voluminous igneous complexes are commonly present in sedimentary basins on volcanic rifted margins, and they represent a challenge for petroleum explorationists. A 2500 km2 industry-standard 3D seismic cube has recently been acquired on the Vøring Marginal High offshore mid-Norway to image subbasalt sedimentary rocks. This cube also provides a unique opportunity for imaging top- and intrabasalt structures. Detailed seismic geomorphological interpretation of the top-basalt horizon, locally calibrated with high-resolution P-Cable wide-azimuth data, reveals new insight into the late-stage development of the volcanic flow fields and the kilometer-high coastal Vøring Escarpment. Subaerial lava flows with compressional ridges and inflated lava lobes cover the marginal high, with a comparable structure and size to modern subaerial lava fields. Pitted surfaces, likely formed by lava emplaced in a wet environment, are present in the western part of the study area near the continent-ocean boundary. The prominent Vøring Escarpment formed when eastward-flowing lava reached the coastline. The escarpment morphology is influenced by preexisting structural highs, and these highs are locally bypassed by the lava. Volcanogenic debris flows are well-imaged on the escarpment horizon, along with large-scale large slump blocks. Similar features exist in active volcanic environments, e.g., on the south coast of Hawaii. Numerous postvolcanic extensional faults and incised channels cut into the marginal high and the escarpment, and we found that the area was geologically active after the volcanism ceased. In summary, igneous seismic geomorphology and seismic volcanostratigraphy are two very powerful methods to understand the volcanic deposits and development of rifted margins. Our study demonstrates great promise for further understanding the igneous development of offshore basins as more high-quality 3D seismic data become available