Computer Simulation Model of the Immune Response of Mice to Western Equine Encephalitis (WEE) Computer Simulation Model of the Immune Response of Mice to Western Equine Encephalitis (WEE) Virusirus

The lymphatic system of an animal is optimally developed to protect the body from antigenic invasion. The epithelial and subepithelial compartments of the body are provided with a vast number of wandering cells and a network of centralized lymphorecticular tissues. All of these are capable of reacting to intruding foreign antigens. Upon antigentic challenge, immunocompetent cells (ICC) or antigen specific lymphocytes are activated; this leads either to cell mediated and/or humoral antibody immune responses. The subsequent effect is an accumulation of the responding cells, both by local proliferation and recruitment from the circulation. Through the interaction of both cellular and humoral components of the immune system, the phagocytic cells and the complement system are activated, thereby leading to the localization, destruction, and elimination of the actigen. Collectively, this set of phenomena is called an immune response. In the course of this response, immunologically committed cells are released from initially activated lymphoid tissue via the efferent lymphatic ducts and the bloodstream to other lymphoid organs with resulting logarithmic amplification of the immune response and the development of the capacity to respond much more efficiently to the second encounter with the same antigen. The immune response to most pathogenic antigens (such as bacterial, fungal, and viral infections) cannot be initiated without the complex reaction of phagocytosis by the macrophages and, probably, by the monocytes

Dichotomous development of the gut microbiome in preterm infants.

BackgroundPreterm infants are at risk of developing intestinal dysbiosis with an increased proportion of Gammaproteobacteria. In this study, we sought the clinical determinants of the relative abundance of feces-associated Gammaproteobacteria in very low birth weight (VLBW) infants. Fecal microbiome was characterized at ≤ 2 weeks and during the 3rd and 4th weeks after birth, by 16S rRNA amplicon sequencing. Maternal and infant clinical characteristics were extracted from electronic medical records. Data were analyzed by linear mixed modeling and linear regression.ResultsClinical data and fecal microbiome profiles of 45 VLBW infants (gestational age 27.9 ± 2.2 weeks; birth weight 1126 ± 208 g) were studied. Three stool samples were analyzed for each infant at mean postnatal ages of 9.9 ± 3, 20.7 ± 4.1, and 29.4 ± 4.9 days. The average relative abundance of Gammaproteobacteria was 42.5% (0-90%) at ≤ 2 weeks, 69.7% (29.9-86.9%) in the 3rd, and 75.5% (54.5-86%) in the 4th week (p < 0.001). Hierarchical and K-means clustering identified two distinct subgroups: cluster 1 started with comparatively low abundance that increased with time, whereas cluster 2 began with a greater abundance at ≤ 2 weeks (p < 0.001) that decreased over time. Both groups resembled each other by the 3rd week. Single variants of Klebsiella and Staphylococcus described variance in community structure between clusters and were shared between all infants, suggesting a common, hospital-derived source. Fecal Gammaproteobacteria was positively associated with vaginal delivery and antenatal steroids.ConclusionsWe detected a dichotomy in gut microbiome assembly in preterm infants: some preterm infants started with low relative gammaproteobacterial abundance in stool that increased as a function of postnatal age, whereas others began with and maintained high abundance. Vaginal birth and antenatal steroids were identified as predictors of Gammaproteobacteria abundance in the early (≤ 2 weeks) and later (3rd and 4th weeks) stool samples, respectively. These findings are important in understanding the development of the gut microbiome in premature infants

Lowest Open Channels, Bound States, and Narrow Resonances of Dipositronium

The constraints imposed by symmetry on the open channels of dipositronium has been studied, and the symmetry-adapted lowest open channel of each quantum state has been identified. Based on this study, the existence of two more 0^+ bound states has been theoretically confirmed, and a 0^+ narrow resonance has been predicted. A variational calculation has been performed to evaluate the critical strength of the repulsive interaction . Two 0^- states are found to have their critical strengths very close to 1, they are considered as candidates of new narrow resonances or loosely bound states .Comment: 10 pages, 0 figure

Bridging Molecular Genetics and Biomarkers in Lewy Body and Related Disorders

Recent advances have been made in defining the genetic and molecular basis of dementia with Lewy bodies (DLBs) and related neurodegenerative disorders such as Parkinson's disease (PD) and Parkinson's disease dementia (PDD) which comprise the spectrum of “Lewy body disorders” (LBDs). The genetic alterations and underlying disease mechanisms in the LBD overlap substantially, suggesting common disease mechanisms. As with the other neurodegenerative dementias, early diagnosis in LBD or even identification prior to symptom onset is key to developing effective therapeutic strategies, but this is dependent upon the development of robust, specific, and sensitive biomarkers as diagnostic tools and therapeutic endpoints. Recently identified mutations in the synucleins and other relevant genes in PD and DLB as well as related biomolecular pathways suggest candidate markers from biological fluids and imaging modalities that reflect the underlying disease mechanisms. In this context, several promising biomarkers for the LBD have already been identified and examined, while other intriguing possible candidates have recently emerged. Challenges remain in defining their correlation with pathological processes and their ability to detect DLB and related disorders, and perhaps a combined array of biomarkers may be needed to distinguish various LBDs

Mass flow rate measurement of thermal creep flow from transitional to slip flow regime

Measurements of the thermal creep flow through a single rectangular microchannel connected to two tanks maintained initially at the same pressure, but at different temperatures, are carried out for five noble gas species, over a large range of pressure and for two temperature differences between the tanks. The time-dependent pressure variations in both cold and hot tanks are investigated, and the temperature-driven (thermal creep) mass flow rate between two tanks is calculated from these data for the rarefaction parameter ranging from the transitional to slip flow regime. The measured mass flow rate is compared with the numerical solution of the S-model kinetic equation, and they show good agreement. A novel approximate expression to calculate the temperature-driven mass flow rate in the transitional and slip flow regimes is proposed. This expression provides results in good agreement with the measured values of the mass flow rate. In the slip flow regime, the thermal slip coefficient is calculated by employing the previously reported methodology, and the influence of the nature of the gas on this coefficient is investigated. The measured values of the thermal slip coefficient agree well with the values available in the literature, indicating that this coefficient is independent of the shape of a channel

The Super Star Cluster NGC 1569-A Resolved on Sub-Parsec Scales with Hubble Space Telescope Spectroscopy

We present 3000--10000 Ang HST/STIS long-slit spectroscopy of the bright super star cluster A (SSC-A) in the dwarf starburst galaxy NGC 1569. The 0.05" HST angular resolution allows, for the first time, to probe for spatial variations in the stellar population of a ~ 10^6 M_sun SSC. Integrated ground-based spectra of SSC-A have previously revealed young Wolf-Rayet (WR) signatures that coexist with features from supposedly older, red supergiant (RSG), populations. We find that the WR emission complexes come solely from the subcluster A2, identified in previous HST imaging, and are absent from the main cluster A1, thus resolving the question of whether the WR and RSG features arise in a single or distinct clusters. The equivalent widths of the WR features in A2 --- including the CIV 5808 complex which we detect in this object for the first time --- are larger than previously observed in other WR galaxies. Models with sub-solar metallicity, as inferred from the nebular emission lines of this galaxy, predict much lower equivalent widths. On the ``clean'' side of A1, opposite to A2, we find no evidence for radial gradients in the observed stellar population at 0.05"<R<0.40" (~0.5 to 5 pc), neither in broad-band, low-resolution, spectra nor in medium-resolution spectra of the infrared CaII triplet.Comment: 5 pages, accepted for publication in ApJ Le

Elastic biodegradable starch/ethylene-co-vinyl alcohol fibre-mesh scaffolds for tissue engineering applications

The fabrication of a biomaterial scaffold, with adequate physical and structural properties for tissue engineering applications, is reported. A blend of starch with ethylene-vinyl alcohol (50/50 w/w, SEVA-C) is used to produce 3D fibre-mesh scaffolds by wet-spinning. The scaffolds are characterized in terms of morphology, porosity, interconnectivity, and pore size, using scanning electron microscopy (SEM) and microcomputed tomography (μCT). The degradation behavior, as well as the mechanical properties of the scaffolds, is investigated in presence of alpha-amylase enzyme at physiological concentration. Scaffolds with porosities ranging from 43 to 52%, interconnectivity of ∼70.5% and pore size between 118 and 159 μm, can be fabricated using the proposed methodology. The scaffolds exhibit an elastic behavior in the wet state with a compressive modulus of 7.96±0.32 MPa. Degradation studies show that SEVA-C scaffolds are susceptible to enzymatic degradation by alpha-amylase, confirmed by the increase of weight loss (40% of weight loss after 12 weeks) and presence of degradation products (reducing sugars) in solution. The diameter of SEVA-C scaffolds decreases with degradation time, increasing the overall porosity, interconnectivity and pore size. In vitro cell studies with human osteosarcoma cell line (SaOs-2) showed a nontoxic and cytocompatible behavior of the developed fibre mesh scaffolds. The positive cellular response, together with structural and degradable properties, suggests that 3D SEVA-C fibre-meshes may be good candidates as tissue engineering scaffolds. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40504. Copyright © 2014 Wiley Periodicals, Inc.This work was supported by national funds through the Portuguese Foundation for Science and Technology under the scope of the project PTDC/CTM/67560/2006 and by the European Regional Development Fund (ERDF) through the Operational Competitiveness Programme “COMPETE” (FCOMP-01-0124-FEDER-007148)

Detection of Coherent Vorticity Structures using Time-Scale Resolved Acoustic Spectroscopy

We describe here an experimental technique based on the acoustic scattering phenomenon allowing the direct probing of the vorticity field in a turbulent flow. Using time-frequency distributions, recently introduced in signal analysis theory, for the analysis of the scattered acoustic signals, we show how the legibility of these signals is significantly improved (time resolved spectroscopy). The method is illustrated on data extracted from a highly turbulent jet flow : discrete vorticity events are clearly evidenced. We claim that the recourse to time-frequency distributions lead to an operational definition of coherent structures associated with phase stationarity in the time-frequency plane.Comment: 26 pages, 6 figures. Latex2e format Revised version : Added references, figures and Changed conten