Towards a positive welfare protocol for cattle: A critical review of indicators and suggestion of how we might proceed

Current animal welfare protocols focus on demonstrating the absence (or at least low levels) of indicators of poor welfare, potentially creating a mismatch between what is expected by society (an assurance of good animal welfare) and what is actually being delivered (an assurance of the absence of welfare problems). This paper explores how far we have come, and what work still needs to be done, if we are to develop a protocol for use on commercial dairy farms where the aim is to demonstrate the presence of positive welfare. Following conceptual considerations around a perceived “ideal” protocol, we propose that a future protocol should be constructed (i) of animal-based measures, (ii) of indicators of affective state, and (iii) be structured according to indicators of short-term emotion, medium-term moods and long-term cumulative assessment of negative and positive experiences of an animal's life until now (in contrast to the current focus on indicators that represent different domains/criteria of welfare). These three conditions imposed the overall structure within which we selected our indicators. The paper includes a critical review of the literature on potential indicators of positive affective states in cattle. Based on evidence about the validity and reliability of the different indicators, we select ear position, play, allogrooming, brush use and QBA as candidate indicators that we suggest could form a prototype positive welfare protocol. We emphasise that this prototype protocol has not been tested in practice and so it is perhaps not the protocol itself that is the main outcome of this paper, but the process of trying to develop it. In a final section of this paper, we reflect on some of the lessons learnt from this exercise and speculate on future perspectives. For example, while we consider we have moved towards a prototype positive welfare protocol for short-term affective states, future research energy should be directed towards valid indicators for the medium and long-term

For their own good? The unseen harms of disenhancing farmed animals

In recent years, some ethicists have defended that we should genetically engineer farmed animals to diminish or eliminate their capacity to experience negative affective states, a process known as disenhancement that would, according to these authors, result in a situation that is better than the status quo. While we agree with this overall assessment, we believe that it is a mistake to defend disenhancement as a good solution to farmed animals’ plight. This is because disenhancement entails some generally unseen harms that arise from the fact that negative affective states, despite feeling bad, support the access to a number of intrinsic goods, such as individuality, social relationships, meaning, and political participation. Though farmed animals currently have few opportunities to enjoy these goods, we argue that this is a reason to change the environment in which they are kept, not the animals. If we truly care about improving farmed animals’ lives, we should aim to enrich their environment, rather than impoverish their mental lives

Sex and Age Don't Matter, but Breed Type Does—Factors Influencing Eye Wrinkle Expression in Horses

Identifying valid indicators to assess animals' emotional states is a critical objective of animal welfare science. In horses, eye wrinkles above the eyeball have been shown to be affected by pain and other emotional states. From other species we know that individual characteristics, e.g., age in humans, affect facial wrinkles, but it has not yet been investigated whether eye wrinkle expression in horses is systematically affected by such characteristics. Therefore, the aim of this study was to assess how age, sex, breed type, body condition, and coat colour affect the expression and/or the assessment of eye wrinkles in horses. To this end, we adapted the eye wrinkle assessment scale from Hintze et al. (1) and assessed eye wrinkle expression in pictures taken from the left and the right eye of 181 horses in a presumably neutral situation, using five outcome measures: a qualitative first impression reflecting how worried the horse is perceived by humans, the extent to which the brow is raised, the number of wrinkles, their markedness and the angle between a line through both corners of the eye and the topmost wrinkle. All measures could be assessed highly reliable with respect to intra- and inter-observer agreement. Breed type affected the width of the angle [F(2,114) = 8.20, p < 0.001], with thoroughbreds having the narrowest angle (M = 23.80, SD = 1.60), followed by warmbloods (M = 28.00, SD = 0.60), and coldbloods (M = 31.00, SD = 0.90). None of the other characteristics affected any of the outcome measures, and eye wrinkle expression did not differ between the left and the right eye area (all p-values > 0.05). In conclusion, horses' eye wrinkle expression and its assessment in neutral situations was not systematically affected by the investigated characteristics, except for “breed type”, which accounted for some variation in “angle”; how much eye wrinkle expression is affected by emotion or perhaps mood needs further investigation and validation

Maternal separation followed by isolation-housing differentially affects prepulse inhibition of the acoustic startle response in C57BL/6 mice

Exposure to chronic stress is associated with an increased incidence of neuropsychiatric dysfunction. The current study evaluated two competing hypotheses, the cumulative stress and the match/mismatch hypothesis of neuropsychiatric dysfunction, using two paradigms relating to exposure to “stress”: pre-weaning maternal separation and post-weaning isolation-housing. C57BL/6 offspring were reared under four conditions: typical animal facility rearing (AFR, control), early handling (EH, daily 15 min separation from dam), maternal separation (MS, daily 4 hr separation from dam), and maternal and peer separation (MPS, daily 4 hr separation from dam and from littermates). After weaning, mice were either housed socially (2–3/cage) or in isolation (1/cage) and then tested for prepulse inhibition in adulthood. Isolation-housed MPS subjects displayed greater deficits in prepulse inhibition relative to socially-housed MPS subjects while socially-housed AFR subjects displayed greater deficits in prepulse inhibition relative to isolation-housed AFR subjects. The results indicate that these treatment conditions represent a potentially valuable model for evaluating the match/mismatch hypothesis in regards to neuropsychiatric dysfunction

Effects of Cage Enrichment on Behavior, Welfare and Outcome Variability in Female Mice

The manner in which laboratory rodents are housed is driven by economics (minimal use of space and resources), ergonomics (ease of handling and visibility of animals), hygiene, and standardization (reduction of variation). This has resulted in housing conditions that lack sensory and motor stimulation and restrict the expression of species-typical behavior. In mice, such housing conditions have been associated with indicators of impaired welfare, including abnormal repetitive behavior (stereotypies, compulsive behavior), enhanced anxiety and stress reactivity, and thermal stress. However, due to concerns that more complex environmental conditions might increase variation in experimental results, there has been considerable resistance to the implementation of environmental enrichment beyond the provision of nesting material. Here, using 96 C57BL/6 and SWISS female mice, respectively, we systematically varied environmental enrichment across four levels spanning the range of common enrichment strategies: (1) bedding alone; (2) bedding + nesting material; (3) deeper bedding + nesting material + shelter + increased vertical space; and (4) semi-naturalistic conditions, including weekly changes of enrichment items. We studied how these different forms of environmental enrichment affected measures of animal welfare, including home-cage behavior (time–budget and stereotypic behavior), anxiety (open field behavior, elevated plus-maze behavior), growth (food and water intake, body mass), stress physiology (glucocorticoid metabolites in fecal boluses and adrenal mass), brain function (recurrent perseveration in a two-choice guessing task) and emotional valence (judgment bias). Our results highlight the difficulty in making general recommendations across common strains of mice and for selecting enrichment strategies within specific strains. Overall, the greatest benefit was observed in animals housed with the greatest degree of enrichment. Thus, in the super-enriched housing condition, stereotypic behavior, behavioral measures of anxiety, growth and stress physiology varied in a manner consistent with improved animal welfare compared to the other housing conditions with less enrichment. Similar to other studies, we found no evidence, in the measures assessed here, that environmental enrichment increased variation in experimental results

Diagnosing mucopolysaccharidosis IVA

Mucopolysaccharidosis IVA (MPS IVA; Morquio A syndrome) is an autosomal recessive lysosomal storage disorder resulting from a deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) activity. Diagnosis can be challenging and requires agreement of clinical, radiographic, and laboratory findings. A group of biochemical genetics laboratory directors and clinicians involved in the diagnosis of MPS IVA, convened by BioMarin Pharmaceutical Inc., met to develop recommendations for diagnosis. The following conclusions were reached. Due to the wide variation and subtleties of radiographic findings, imaging of multiple body regions is recommended. Urinary glycosaminoglycan analysis is particularly problematic for MPS IVA and it is strongly recommended to proceed to enzyme activity testing even if urine appears normal when there is clinical suspicion of MPS IVA. Enzyme activity testing of GALNS is essential in diagnosing MPS IVA. Additional analyses to confirm sample integrity and rule out MPS IVB, multiple sulfatase deficiency, and mucolipidoses types II/III are critical as part of enzyme activity testing. Leukocytes or cultured dermal fibroblasts are strongly recommended for enzyme activity testing to confirm screening results. Molecular testing may also be used to confirm the diagnosis in many patients. However, two known or probable causative mutations may not be identified in all cases of MPS IVA. A diagnostic testing algorithm is presented which attempts to streamline this complex testing process

Eye wrinkle expression in horses

Eye wrinkle expression in horse

Sex and Age Don't Matter, but Breed Type Does—Factors Influencing Eye Wrinkle Expression in Horses

Identifying valid indicators to assess animals' emotional states is a critical objective of animal welfare science. In horses, eye wrinkles above the eyeball have been shown to be affected by pain and other emotional states. From other species we know that individual characteristics, e.g., age in humans, affect facial wrinkles, but it has not yet been investigated whether eye wrinkle expression in horses is systematically affected by such characteristics. Therefore, the aim of this study was to assess how age, sex, breed type, body condition, and coat colour affect the expression and/or the assessment of eye wrinkles in horses. To this end, we adapted the eye wrinkle assessment scale from Hintze et al. (1) and assessed eye wrinkle expression in pictures taken from the left and the right eye of 181 horses in a presumably neutral situation, using five outcome measures: a qualitative first impression reflecting how worried the horse is perceived by humans, the extent to which the brow is raised, the number of wrinkles, their markedness and the angle between a line through both corners of the eye and the topmost wrinkle. All measures could be assessed highly reliable with respect to intra- and inter-observer agreement. Breed type affected the width of the angle [F-(2 ,F- 114) = 8.20, p < 0.001], with thoroughbreds having the narrowest angle (M = 23.80, SD = 1.60), followed by warmbloods (M = 28.00, SD = 0.60), and coldbloods (M = 31.00, SD = 0.90). None of the other characteristics affected any of the outcome measures, and eye wrinkle expression did not differ between the left and the right eye area (all p-values 0.05). In conclusion, horses' eye wrinkle expression and its assessment in neutral situations was not systematically affected by the investigated characteristics, except for "breed type", which accounted for some variation in "angle"; how much eye wrinkle expression is affected by emotion or perhaps mood needs further investigation and validation

Cattle or no cattle? Presence of cattleon pasture does not affect fearfulness in broiler chickens

Keeping broiler chickens on pasture with cattle may increase range use of the chickens and reduce predation by predatory birds. Previous studies suggest that increased range use is associated with shorter durations spent in Tonic Immobility (TI), a measure of fearfulness. Does duration spent in TI differ between broilers housed with or without young cattle on pasture? During two years, three batches of broilers (approx. 110-120 broilers each) were TI tested at four weeks of age and then allocated to control (only pasture) and treatment (pasture with ten cattle) balanced for seconds in TI. All broilers were kept for six weeks on pasture with or without cattle and were then TI tested again. TI tests were performed by one handler. Broilers were carried individually to the testing area, turned on their back and held with one hand over the head and one on the sternum for 10 s. The time until the broiler righted itself was measured as time in TI (max. 600 s). If a broiler righted itself earlier than 10 s after release, TI was not induced and the described process was repeated (max. 5 attempts). Number of attempts necessary to induce TI and duration in TI were compared between treatment and control group after six weeks on pasture. Due to the small number of replicates (at group level) we used descriptive statistics and visual inspection for interpretation. Duration in TI was lower in broilers ranging with cattle (mean ± sd 139 s ± 150 s) compared to broilers ranging with conspecifics only (150 s ± 146 s), but number of attempts did not differ (2 ± 1). Due to the small difference (10 s) found, we conclude that the presence of cattle did not influence the fearfulness measured by number of attempts and duration in TI

Single or mixed: Comparing behaviour of single- and multi-species groups of young cattle and broiler chickens on pasture

The practice of keeping two or more species together has received little attention in research. Here we present a first explorative study comparing behaviour on pasture in multi-species groups of cattle and broiler chickens to single-species groups. In four 6-week cycles, two single-species groups (ten cattle and approximately 55 broilers, respectively)and one multi-species group (ten cattle with approximately 55 broilers) were observed on pasture. Twice a week, once in the morning and once in the evening, ten animals per species and group were directly observed using focal animal sampling for 6 minutes each. Behaviours including e.g. feeding, locomotion and interactions were recorded as frequencies or durations. Inter-observer-agreement between two independent observers was moderate to high. Due to the small sample size (n = 4 cycles) we present our results descriptively. Cattle in multi-species groups spent on average 20 % ± 39 (mean, sd) of the time lying, while the percentage of lying time amounted to 30 % ± 42 in single species groups. Broilers in multi-species groups were out of sight (i.e., in hut)for 16 % ± 32 of the time and broilers in single-species groups for 25 % ± 36. Cattle in multi-species groups were feeding 63 % ± 41 (single-specie groups: 60 % ± 42), standing 9 % ± 2 (6 % ± 2) of their time and interacting with conspecifics 7 ± 13 (6 ± 13) times per hour. Broilers in multi-species groups were lying 19 % ± 30 (single species groups: 14 % ± 29) and foraging 50 % ± 36 (48 % ± 36) of their time and performed comfort behaviour 2 ± 5 (1 ± 5)times per hour. While we cannot draw conclusions based on statistical differences, these first findings indicate that animals in mixed groups may influence the behaviour of the other species. For example, broilers may perceive cattle as structural elements of the pasture and therefore spent more time outside and less time in the hut than broilers in single-species groups. Cattle in multi-species groups may be lying less than cattle in single-species groups, due to the activity of the broilers. However, further research is necessary to confirm these first results and to investigate the reasons for such differences