5,533 research outputs found
Use of multiple primers in RAPD analysis of clonal organisms provides limited improvement in discrimination
Randomly amplified polymorphic DNA (RAPD) analysis using two or more primers has been reported to provide additional discriminatory ability over one primer used individually. This may be of particular application in epidemiological typing of clonal organisms, such as Shiga toxin-producing E. coli O157, where strain differentiation can be difficult. Using four arbitrary primers individually, and in all possible permutations, E. coli O157 isolates and other arbitrarily chosen E. coli strains were typed using RAPD analysis. For most nonclonal strains, the use of two primers resulted in increased differentiation between isolates; however, more than two primers did not increase further the discriminatory capacity. E. coli O157 isolates that produced virtually identical profiles using one primer did not show increased differentiation when using two or more primers, demonstrating that in some cases, where strains of an organism are highly related, there is limited advantage to using more than one primer in RAPD analysis
Noise reduction studies for the OV-1 airplane
A study has been conducted to define possible modifications to the OV-1 aircraft to reduce its aural detection distance. This effort involved documenting the noise characteristics of the airplane, devising modifications to reduce the noise, estimating the reduction in detection distance, and evaluating aircraft performance as a result of these modifications. It was found that the main noise source on this aircraft is the propeller and hence modifications only to the propeller and the propeller drive system are proposed. Modifications involving only the propeller are noted to involve no increase in weight but they result in only a modest decrease in aural detection distance. In order to obtain substantial decreases in aural detection distance, modifications involving changes both to the propeller and the engine-propeller gearing are required
Spin injection from perpendicular magnetized ferromagnetic -MnGa into (Al,Ga)As heterostructures
Electrical spin injection from ferromagnetic -MnGa into an (Al,Ga)As
p-i-n light emitting diode (LED) is demonstrated. The -MnGa layers show
strong perpendicular magnetocrystalline anisotropy, enabling detection of spin
injection at remanence without an applied magnetic field. The bias and
temperature dependence of the spin injection are found to be qualitatively
similar to Fe-based spin LED devices. A Hanle effect is observed and
demonstrates complete depolarization of spins in the semiconductor in a
transverse magnetic field.Comment: 4 pages, 3 figure
Time-division SQUID multiplexers with reduced sensitivity to external magnetic fields
Time-division SQUID multiplexers are used in many applications that require
exquisite control of systematic error. One potential source of systematic error
is the pickup of external magnetic fields in the multiplexer. We present
measurements of the field sensitivity figure of merit, effective area, for both
the first stage and second stage SQUID amplifiers in three NIST SQUID
multiplexer designs. These designs include a new variety with improved
gradiometry that significantly reduces the effective area of both the first and
second stage SQUID amplifiers.Comment: 4 pages, 7 figures. Submitted for publication in the IEEE
Transactions on Applied Superconductivity, August 201
Noise measurement evaluations of various takeoff-climbout profiles of a four-engine turbojet transport airplane
Noise measurement evaluations of various takeoff-climbout profiles of four engine turbojet transport airplan
Creutzfeldt-Jakob disease: a systematic review of global incidence, prevalence, infectivity, and incubation
Creutzfeldt-Jakob disease (CJD) is a fatal disease presenting with rapidly progressive dementia, and most patients die within a year of clinical onset. CJD poses a potential risk of iatrogenic transmission, as it can incubate asymptomatically in humans for decades before becoming clinically apparent. In this Review, we sought evidence to understand the current iatrogenic risk of CJD to public health by examining global evidence on all forms of CJD, including clinical incidence and prevalence of subclinical disease. We found that although CJD, particularly iatrogenic CJD, is rare, the incidence of sporadic CJD is increasing. Incubation periods as long as 40 years have been observed, and all genotypes have now been shown to be susceptible to CJD. Clinicians and surveillance programmes should maintain awareness of CJD to mitigate future incidences of its transmission. Awareness is particularly relevant for sporadic CJD, which occurs in older people in whom clinical presentation could resemble rapidly developing dementia
Possible explanations for different surface quality in laser cutting with 1 micron and 10 microns beams
In laser cutting of thick steel sheets, quality difference is observed between cut surfaces obtained with 1 micron and 10 micron laser beams. This paper investigates physical mechanisms for this interesting and important problem of the wavelength dependence. First, striation generation process is described, based on a 3D structure of melt flow on a kerf front, which was revealed for the first time by our recent experimental observations. Two fundamental processes are suggested to explain the difference in the cut surface quality: destabilization of the melt flow in the central part of the kerf front and downward displacement of discrete melt accumulations along the side parts of the front. Then each of the processes is analyzed using a simplified analytical model. The results show that in both processes, different angular dependence of the absorptivity of the laser beam can result in the quality difference. Finally we propose use of radial polarization to improve the quality with the 1 micron wavelength
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