A Mother’s Sacrifice: The contribution of asymmetric cell division to lifespan regulation in Saccharomyces cerevisiae.

Aging determinants are asymmetrically distributed during cell division in S. cerevisiae, which leads to production of an immaculate, age-free daughter cell. During this process, damaged components are sequestered and retained in the mother cell, while higher functioning organelles and rejuvenating factors are transported to and/or enriched in the bud. Here, we will describe the key quality control mechanisms in budding yeast that contribute to asymmetric cell division of aging determinants, with a specific focus on mitochondria. We find that the actin cytoskeleton, which drives transport of many cellular components in yeast, plays a crucial role in segregating fit from less fit mitochondria between mother and daughter cells. Since actin cables are dynamic structures that undergo retrograde flow, treadmilling from the bud towards the mother cell, they acts as filters to prevent damaged, dysfunctional mitochondria from being inherited by the daughter cell. This asymmetry has a direct impact on regulation of daughter cell fitness. A direct counterpart to mitochondrial motility events is anchorage of the organelle, which occurs in the mother tip, mother cortex, and bud tip in budding yeast. We find that mitochondrial fusion, together with tethering protein, serves to promote anchorage and accumulation of mitochondria at the bud tip. This anchorage must be properly maintained, as ectopic increase in mitochondrial anchorage can disrupt quality control mechanisms aimed at promoting asymmetric cell division

Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae

Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. However, the availability of optimal fluorescent protein combinations for 3-color imaging is limited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with conventional blue dyes, such as DAPI, for potential four-color live cell imaging

Joint analysis of the energy spectrum of ultra-high-energy cosmic rays measured at the Pierre Auger Observatory and the Telescope Array

The measurement of the energy spectrum of ultra-high-energy cosmic rays (UHECRs) is of crucial importance to clarify their origin and acceleration mechanisms. The Pierre Auger Observatory in Argentina and the Telescope Array (TA) in the US have reported their measurements of UHECR energy spectra observed in the southern and northern hemisphere, respectively. The region of the sky accessible to both Observatories ([−15,+24] degrees in declination) can be used to cross-calibrate the two spectra. The Auger-TA energy spectrum working group was organized in 2012 and has been working to understand the uncertainties in energy scale in both experiments, their systematic differences, and differences in the shape of the spectra. In previous works, we reported that there was an overall agreement of the energy spectra measured by the two observatories below 10 EeV while at higher energies, a remaining significant difference was observed in the common declination band. We revisit this issue to understand its origin by examining the systematic uncertainties, statistical effects, and other possibilities. We will also discuss the differences in the spectra in different declination bands and a new feature in the spectrum recently reported by the Auger Collaboration

The UHECR dipole and quadrupole in the latest data from the original Auger and TA surface detectors

The sources of ultra-high-energy cosmic rays are still unknown, but assuming standard physics, they are expected to lie within a few hundred megaparsecs from us. Indeed, over cosmological distances cosmic rays lose energy to interactions with background photons, at a rate depending on their mass number and energy and properties of photonuclear interactions and photon backgrounds. The universe is not homogeneous at such scales, hence the distribution of the arrival directions of cosmic rays is expected to reflect the inhomogeneities in the distribution of galaxies; the shorter the energy loss lengths, the stronger the expected anisotropies. Galactic and intergalactic magnetic fields can blur and distort the picture, but the magnitudes of the largest-scale anisotropies, namely the dipole and quadrupole moments, are the most robust to their effects. Measuring them with no bias regardless of any higher-order multipoles is not possible except with full-sky coverage. In this work, we achieve this in three energy ranges (approximately 8--16 EeV, 16--32 EeV, and 32--∞ EeV) by combining surface-detector data collected at the Pierre Auger Observatory until 2020 and at the Telescope Array (TA) until 2019, before the completion of the upgrades of the arrays with new scintillator detectors. We find that the full-sky coverage achieved by combining Auger and TA data reduces the uncertainties on the north-south components of the dipole and quadrupole in half compared to Auger-only results

Joint analysis of the energy spectrum of ultra-high-energy cosmic rays measured at the Pierre Auger Observatory and the Telescope Array

The measurement of the energy spectrum of ultra-high-energy cosmic rays (UHECRs) is of crucial importance to clarify their origin and acceleration mechanisms. The Pierre Auger Observatory in Argentina and the Telescope Array (TA) in the US have reported their measurements of UHECR energy spectra observed in the southern and northern hemisphere, respectively. The region of the sky accessible to both Observatories ([-15, +24] degrees in declination) can be used to cross-calibrate the two spectra. The Auger-TA energy spectrum working group was organized in 2012 and has been working to understand the uncertainties in energy scale in both experiments, their systematic differences, and differences in the shape of the spectra. In previous works, we reported that there was an overall agreement of the energy spectra measured by the two observatories below 10 EeV while at higher energies, a remaining significant difference was observed in the common declination band. We revisit this issue to understand its origin by examining the systematic uncertainties, statistical effects, and other possibilities. We will also discuss the differences in the spectra in different declination bands and a new feature in the spectrum recently reported by the Auger Collaboration

The UHECR dipole and quadrupole in the latest data from the original Auger and TA surface detectors

The sources of ultra-high-energy cosmic rays are still unknown, but assuming standard physics, they are expected to lie within a few hundred megaparsecs from us. Indeed, over cosmological distances cosmic rays lose energy to interactions with background photons, at a rate depending on their mass number and energy and properties of photonuclear interactions and photon backgrounds. The universe is not homogeneous at such scales, hence the distribution of the arrival directions of cosmic rays is expected to reflect the inhomogeneities in the distribution of galaxies; the shorter the energy loss lengths, the stronger the expected anisotropies. Galactic and intergalactic magnetic fields can blur and distort the picture, but the magnitudes of the largest-scale anisotropies, namely the dipole and quadrupole moments, are the most robust to their effects. Measuring them with no bias regardless of any higher-order multipoles is not possible except with full-sky coverage. In this work, we achieve this in three energy ranges (approximately 8--16 EeV, 16--32 EeV, and 32--∞ EeV) by combining surface-detector data collected at the Pierre Auger Observatory until 2020 and at the Telescope Array (TA) until 2019, before the completion of the upgrades of the arrays with new scintillator detectors. We find that the full-sky coverage achieved by combining Auger and TA data reduces the uncertainties on the north-south components of the dipole and quadrupole in half compared to Auger-only results

UHECR arrival directions in the latest data from the original Auger and TA surface detectors and nearby galaxies

The distribution of ultra-high-energy cosmic-ray arrival directions appears to be nearly isotropic except for a dipole moment of order $6 \times (E/10~\mathrm{EeV})$ per cent. Nonetheless, at the highest energies, as the number of possible candidate sources within the propagation horizon and the magnetic deflections both shrink, smaller-scale anisotropies might be expected to emerge. On the other hand, the flux suppression reduces the statistics available for searching for such anisotropies. In this work, we consider two different lists of candidate sources: a sample of nearby starburst galaxies and the 2MRS catalog tracing stellar mass within 250 Mpc. We combine surface-detector data collected at the Pierre Auger Observatory until 2020 and the Telescope Array until 2019, and use them to test models in which UHECRs comprise an isotropic background and a foreground originating from the candidate sources and randomly deflected by magnetic fields. The free parameters of these models are the energy threshold, the signal fraction, and the search angular scale. We find a correlation between the arrival directions of $11.8\%_{-3.1\%}^{+5.0\%}$ of cosmic rays detected with $E \ge 38~\mathrm{EeV}$ by Auger or with $E \gtrsim 49~\mathrm{EeV}$ by TA and the position of nearby starburst galaxies on a ${15.5^\circ}_{-3.2^\circ}^{+5.3^\circ}$ angular scale, with a 4.2σ post-trial significance, as well as a weaker correlation with the overall galaxy distribution

UHECR arrival directions in the latest data from the original Auger and TA surface detectors and nearby galaxies

The distribution of ultra-high-energy cosmic-ray arrival directions appears to be nearly isotropic except for a dipole moment of order 6× (E/10 EeV) per cent. Nonetheless, at the highest energies, as the number of possible candidate sources within the propagation horizon and the magnetic deflections both shrink, smaller-scale anisotropies might be expected to emerge. On the other hand, the flux suppression reduces the statistics available for searching for such anisotropies. In this work, we consider two different lists of candidate sources: a sample of nearby starburst galaxies and the 2MRS catalog tracing stellar mass within 250 Mpc. We combine surface-detector data collected at the Pierre Auger Observatory until 2020 and the Telescope Array until 2019, and use them to test models in which UHECRs comprise an isotropic background and a foreground originating from the candidate sources and randomly deflected by magnetic fields. The free parameters of these models are the energy threshold, the signal fraction, and the search angular scale. We find a correlation between the arrival directions of 11.8%5.0%-3.1% of cosmic rays detected with E ≥ 38 EeV by Auger or with ≥ 49 EeV by TA and the position of nearby starburst galaxies on a 15.5°+5.3°-3.2° angular scale, with a 4.2f post-trial significance, as well as a weaker correlation with the overall galaxy distribution