Estabilización de las subrasantes loéssicas utilizando el descarte de piedras ornamentales

El trabajo consiste en determinar el porcentaje óptimo de la dosificación del descarte proveniente de los telares de los aserraderos de rocas metamórficas e ígneas ornamentales cordobesas, mezclado con, los sedimentos inorgánicos de la planicie loéssicos erodable de la región mediterránea de la Argentina. Dicha dosificación, a punta a estabilizar química y mecánicamente, las subrasantes no pavimentadas en caminos terciarios con insuficiente bombeo transversal o drenaje lateral de cunetas. Generalmente, son vías de uso estacionales con un tránsito netamente agropecuario y, por ende presentan un escaso a nulo mantenimiento de la superficie de rodamiento; manifestandose, como consecuencia, una disminución paulatina de la resistencia al corte no drenada (Su) como, de la presión soporte (CBR 2,5mm) en cada intercalación anual de precipitaciones y sequias. Por ende, la estabilización del loess erodable con el porcentaje óptimo del descarte a determinar, logra garantizar un incremento de los valores de Su y CBR 2,5mm.en las subrasantes no pavimentadas estudiadas; siendo así optimizado el manteamiento de la superficie de rodamiento en al menos un periodo de precipitaciones y sequias.En general los ensayos geotécnicos realizados en el laboratorio de suelos y materiales de Universidad Católica de Córdoba (UCC) que garantizan dichos objetivos, son: contenido de humedad, lavado tamiz Nº 200, ensayos granulométricos, límites de Atterberg, doble hidrómetro, superficie específica, proctor estándar, compresión simple y California Bearing Ratio

Nursing Care of Women Who Suffered Sexual Violence Atención de enfermería a mujeres que sufren violencia sexual Atendimento de enfermagem às mulheres que sofrem violência sexual

The aim of this study was to characterize the nursing care, provided to women who suffered sexual violence, treated at The Women&#39;s Hospital-CAISM/UNICAMP. A retrospective, descriptive study performed using 146 nursing care records of women attended from June 2006 to May 2007. The results showed that the majority of attendances occurred during the day period, that there was consistency between the interventions and the nursing diagnoses identified, and that women reported having received guidance in accordance with the protocol of the program. The nursing consultation time was greater at night (p=0.0227) and the women frequently understood the health risks resulting from sexual violence (p=0.0072), the use of antiretrovirals according to their daily activities (p=0.0397) and they were more focused on the purpose of the serology (p=0.0351). We concluded that the care provided was shown to be appropriate and of a better quality in the nightshift period.<br>Nuestro objetivo fue caracterizar la asistencia prestada por los enfermeros a las mujeres que sufrieron violencia sexual atendidas en el Hospital de la Mujer-CAISM (UNICAMP). Fue realizado un estudio retrospectivo y descriptivo con 146 fichas de atención de enfermería de mujeres atendidas entre junio de 2006 a mayo de 2007. Los resultados mostraron que la mayoría de las atenciones ocurrieron durante el período diurno, hubo coherencia entre las intervenciones y los diagnósticos de enfermería identificados y las mujeres relataron que recibieron orientaciones de acuerdo con el protocolo del programa. El tiempo de consulta de enfermería fue mayor en el período nocturno (p=0,0227) y se constató que esas mujeres frecuentemente conocían los riesgos de salud provenientes de la violencia sexual (p=0,0072), adecuaban los horarios de los antirretrovirales de acuerdo con sus actividades diarias (p=0,0397) y estaban más orientadas en cuanto a la finalidad de las serologías (p=0,0351). Concluimos que la atención prestada se mostró adecuada y con mejor calidad en el plantón nocturno.<br>O objetivo deste estudo foi caracterizar a assistência de enfermagem prestada pelo enfermeiro às mulheres que sofreram violência sexual, atendidas no Hospital da Mulher -CAISM/Unicamp. Foi realizado estudo retrospectivo e descritivo com 146 fichas de atendimento de enfermagem a mulheres, entre junho de 2006 a maio de 2007. Os resultados mostraram que a maioria dos atendimentos ocorreu durante o período diurno, houve coerência entre as intervenções e os diagnósticos de enfermagem identificados, e as mulheres relataram que receberam orientações de acordo com o protocolo do programa. O tempo da consulta de enfermagem foi maior no período noturno (p=0,0227) e essas mulheres mais frequentemente conheciam os riscos de saúde, decorrentes da violência sexual (p=0,0072), adequaram os horários do antirretrovirais, de acordo com suas atividades diárias (p=0,0397), e estavam mais orientadas quanto à finalidade das sorologias (p=0,0351). Conclui-se que o atendimento prestado mostrou-se adequado e com melhor qualidade no plantão noturno

Detection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism

In view of the importance of venereal transmission of bovine leptospirosis, the objective of the present study was to apply the polymerase chain reaction (PCR) to 26 serovars of Leptospira interrogans, L. borgpetersenii, L. santarosai, L. noguchii and L. biflexa, to determine the detection threshold in semen samples and to evaluate the possibility of differentiation among serovars using 19 restriction endonucleases. The results showed that all serovars were amplified and the detection threshold in semen samples of a bull was 100 bacteria/ml. Using endonucleases we could classify the 26 serovars into eight groups. The present results show that PCR is a method of great potential for the detection of Leptospira spp, at bovine artificial insemination centers. (C) 2000 Elsevier B.V. B.V. All rights reserved

Opinião e conhecimento de pacientes e familiares sobre o uso da eletroconvulsoterapia: implicações para a enfermagem Opinion and knowledge of pacients and their relativies of the electroconvulsive therapy: implication for nursing

Neste trabalho verificou-se a opinião e o conhecimento de pacientes internados em uma unidade psiquiátrica e seus familiares, sobre a aceitação do uso da eletroconvulsoterapia. Através de análise quantitativa constatou-se a aprovação da maioria ao tratamento, apesar do escasso conhecimento sobre este, apresentado pelos indivíduos estudados. Constatou-se também que o conhecimento desse tratamento e a confiança depositada no profissional médico que indica o tratamento, parece influir na opinião dos indivíduos sobre seu uso. Desta forma existe a importância do papel do enfermeiro na educação dos indivíduos em relação à eletroconvulsoterapia.<br>The aim of this report was to verify in-patients and their relative's opinion and knowledge of the use of electroconvulsive therapy. By means of a quantitative analysis, the use of this treatment was shown to be accepted despite the individuals knew little about it. It was observed taht the knowledge of this treatment had influence over individual's opinion about it. Thus nurses play an important role on the instruction of these individuals regarding this treatment

In Vitro

Producción CientíficaPURPOSE: Transplantation of autologous corneal stem cells in not possible in cases of bilateral limbal stem cell deficiency (LSCD). To restore the ocular surface in these patients, an autologous extraocular source of stem cells is desirable to avoid dependence on deceased donor tissue and host immunosuppression of allogenic transplants. While bone marrow-derived mesenchymal stem cells (MSCs) can acquire certain characteristics of corneal epithelial cells, subcutaneous adipose tissue (AT) is more readily available and accessible. The aim of this study was to determine if extraocular human AT-derived MSCs (hAT-MSCs) can acquire in vitro some features of corneal epithelial-like cells. METHODS: hAT-MSCs were isolated from human lipoaspirates and expanded up to 3-4 passages. We studied the immunophenotype of MSCs and demonstrated its multipotent capacity to differentiate towards osteoblasts, adipocytes, and chondrocytes. To test the capacity of differentiation of hAT-MSCs towards corneal epithelial-like cells, hAT-MSCs were cultured on substrata of plastic or collagen IV. We used basal culture medium (BM), BM conditioned with human corneal epithelial cells (HCEcBM), and BM conditioned with limbal fibroblasts (LFcBM). RESULTS: The hAT-MSCs incubated for 15 days with HCEcBM acquired more polygonal and complex morphology as evaluated by phase-contrast microscopy and flow cytometry. Additionally, the expression of transforming growth factor-β receptor CD105 and corneal epithelial marker CK12 got increased as evaluated by flow cytometry, real-time reverse-transcription polymerase chain reaction, western-blot, and immunostaining. These changes were absent in hAT-MSCs incubated with unconditioned BM or with LFcBM. CONCLUSIONS: Corneal epithelial-like cells can be induced from extraocular hAT-MSCs by subjecting them to an in vitro microenvironment containing conditioning signals derived from differentiated human corneal epithelial cells. Our results suggest that hAT-MSCs could provide a novel source of stem cells that hold the potential to restore sight lost in patients suffering from bilateral ocular surface failure due to LSCD.Este trabajo fue financiado con fondos procedentes de: Instituto de Salud Carlos III (CIBER-BBN, CB06/01/003), Ministerio de Ciencia e Innovación (SAF2010-14900), Junta de Castilla y León (SAN126/VA12/09). S.G. participó en la realización de este trabajo con un contrato predoctoral de la Junta de Castilla y León y el Fondo Social Europeo