Towards Uniform Gene Bank Documentation In Europe – The Experience From The EFABISnet Project

In the EFABISnet project, a collaborative effort of EAAP, FAO and partners from 14 European countries, in cooperation with the European Regional Focal Point for Animal Genetic Resources (ERFP), national information systems for monitoring the animal genetic resources on breed level were established in Austria, Cyprus, Estonia, Georgia, Iceland, Ireland, Italy, Netherlands, Slovakia, Slovenia, Switzerland, and United Kingdom. The network was soon extended beyond the project plans, with the establishment of EFABIS databases in Finland, Greece, and Hungary. The network was then complemented by a set of inventories of national gene bank collections to strengthen the documentation of ex situ conservation programmes. These documentation systems were established by the National Focal Points for management of farm animal genetic resources. Here we present the experience gained in establishment of these national inventories of gene banks and their relevance to the Strategic Priority Areas of the Global Plan of Action which could be useful for other areas in the world

Modelling global livestock diversity : a fuzzy cognitive mapping approach

For modelling global trends in agrobiodiversity better insight in the relationship between drivers (and related pressures) and agrobiodiversity is needed. In a previous study of the authors a number of indicators for genetic diversity were proposed as being suitable for modelling. In this working document it was investigated if a global agrobiodiversity map for livestock could be produced based on one of these earlier suggested indicators. The Global Domestic Animal Diversity Information System (DAD-IS) was interrogated for one livestock species (cattle) to investigate whether sufficient data of good quality is available to produce such a global map. Additionally, a fuzzy cognitive mapping approach was used to make a qualitative description of livestock diversity in relation to drivers of change. In the FCM 21 factors were identified by the workshop participants to describe the livestock diversity system, of which 10 appeared to be most influential. For these most important factors a list of relevant (proxy) indicators with their potential for use was suggested. These suggested indicators could be the basis for further research in which the so-called archetype methodology could be used to get insight in hotspots of livestock diversity

Inverting the patient involvement paradigm: defining patient led research.

PLAIN ENGLISH SUMMARY: Patients usually understand their disease and lifestyle needs better than many medical professionals. They also have important ideas about what research would be most beneficial to their lives, especially on how to manage symptoms in a way that improves daily quality of life. In the UK, the National Institute for Health Research has recognised the value of patient insight, and now requires researchers with public funding to involve patients and the public throughout the research process. There are many opportunities for involvement, but these generally focus on improving study design to ensure the trial is acceptable to participants. Some programmes work towards setting research priorities as important to patients, public members, and medical experts, but due to the complexity and cost involved in running clinical trials, the majority of research originates with the pharmaceutical industry or academic institutions. There is a clear mismatch between research ideas that patients prioritise (quality of life), and those actually investigated (drug development).The Patient Led Research Hub (PLRH) is a new initiative hosted by the Cambridge Clinical Trials Unit. The PLRH supports research ideas as proposed by patient organisations, providing resources and expertise in research design and delivery. The PLRH aims to co-produce any technically feasible project, regardless of disease or symptom focus. The proposing patient group maintains ownership of the project with an active role in study management. This method of research has proven to produce credible research studies that are of direct relevance to patients. ABSTRACT: Patient and Public Involvement has become an indispensable and expected component of healthcare research in the United Kingdom, largely driven by the National Institute of Health Research and other research funders. Opportunities for patients to become involved in research abound, and many organisations now have dedicated 'public involvement' teams. However, its value is often questioned amidst criticism of tokenism and the recognition that a mismatch persists between patient priorities and funded research. Although patients are frequently consulted, evidence that their involvement influences the research agenda remains limited. We propose a novel model that allows patients and the public not only to propose research questions, but to design, initiate and deliver their own research with all the necessary support from research professionals. We demonstrate the feasibility and utility of this approach in reporting the establishment, experiences and progress of the Patient Led Research Hub. Using this resource, patient organisations are now able to initiate and conduct rigorous clinical research unfettered by the constraints of academic or economic agendas

Broad iron line in the fast spinning neutron-star system 4U 1636-53

We analysed the X-ray spectra of six observations, simultaneously taken with XMM-Newton and Rossi X-ray Timing Explorer (RXTE), of the neutron-star low-mass X-ray binary 4U 1636-53. The observations cover several states of the source, and therefore a large range of inferred mass accretion rate. These six observations show a broad emission line in the spectrum at around 6.5 keV, likely due to iron. We fitted this line with a set of phenomenological models of a relativistically broadened line, plus a model that accounts for relativistically smeared and ionized reflection from the accretion disc. The latter model includes the incident emission from both the neutron-star surface or boundary layer and the corona that is responsible for the high-energy emission in these systems. From the fits with the reflection model we found that in four out of the six observations the main contribution to the reflected spectrum comes from the neutron-star surface or boundary layer, whereas in the other two observations the main contribution to the reflected spectrum comes from the corona. We found that the relative contribution of these two components is not correlated to the state of the source. From the phenomenological models, we found that the iron line profile is better described by a symmetric, albeit broad, profile. The width of the line cannot be explained only by Compton broadening, and we therefore explored the case of relativistic broadening. We further found that the direct emission from the disc, boundary layer and corona generally evolved in a manner consistent with the standard accretion disc model, with the disc and boundary layer becoming hotter and the disc moving inwards as the source changed from the hard to the soft state. The iron line, however, did not appear to follow the same trend

High-content imaging-based BAC-GFP toxicity pathway reporters to assess chemical adversity liabilities.

Adaptive cellular stress responses are paramount in the healthy control of cell and tissue homeostasis and generally activated during toxicity in a chemical-specific manner. Here, we established a platform containing a panel of distinct adaptive stress response reporter cell lines based on BAC-transgenomics GFP tagging in HepG2 cells. Our current panel of eleven BAC-GFP HepG2 reporters together contains (1) upstream sensors, (2) downstream transcription factors and (3) their respective target genes, representing the oxidative stress response pathway (Keap1/Nrf2/Srxn1), the unfolded protein response in the endoplasmic reticulum (Xbp1/Atf4/BiP/Chop) and the DNA damage response (53bp1/p53/p21). Using automated confocal imaging and quantitative single-cell image analysis, we established that all reporters allowed the time-resolved, sensitive and mode-of-action-specific activation of the individual BAC-GFP reporter cell lines as defined by a panel of pathway-specific training compounds. Implementing the temporal pathway activity information increased the discrimination of training compounds. For a set of >30 hepatotoxicants, the induction of Srxn1, BiP, Chop and p21 BAC-GFP reporters correlated strongly with the transcriptional responses observed in cryopreserved primary human hepatocytes. Together, our data indicate that a phenotypic adaptive stress response profiling platform will allow a high throughput and time-resolved classification of chemical-induced stress responses, thus assisting in the future mechanism-based safety assessment of chemicals.Toxicolog