The effect of abrupt weaning of suckler calves on the plasma concentrations of cortisol, catecholamines, leukocyte, acute-phase proteins and in vitro interferon-gamma production

End of project reportThe objective of this study was to examine the effect of abrupt weaning (inclusive of social group disruption and maternal separation) on the physiological mediators of stress and measures of immune function. Thirty-eight male and 38 female continental calves were habituated to handling for two weeks prior to bleeding. Calves were blocked on sex, weight and breed of dam and randomly assigned, within block, to either a control (cows remain with calves) or abruptly weaned group (calves removed from cows). Animals were separated into the respective treatment groups at weaning (0 h). Calves were bled at – 168 h, 6 h (males only), 24 h, 48 h and 168 h post weaning. At each sampling time an observer scored the behavioural reaction of calves to sampling. Blood samples were analysed for cortisol, catecholamine concentrations (not sampled at –168 h) and in vitro interferon-gamma production, neutrophil :lymphocyte ratio and acute phase protein concentrations. All continuous data were analysed using a split-plot ANOVA, except that collected at 6 h, which was analysed using a single factor ANOVA model. The effects of weaning, calf sex and time and respective interactions were described. Disruption of the established social groups at 0 h, increased (p<0.001) the plasma cortisol concentration and neutrophil: lymphocyte ratio and reduced the leukocyte concentration (p<0.001) and the in vitro interferon-gamma response to the mitogen concanavalin-A (p<0.001) and keyhole limpet haemocyanin (p<0.001) for weaned and control animals, when compared with –168h. Plasma adrenaline and noradrenaline concentrations were not affected by group disruption. There was no effect of weaning or sex on calf behavioural reaction to handling during blood sampling. Plasma cortisol and adrenaline concentrations were not affected by weaning or sex. Plasma noradrenaline concentration was influenced by weaning x sex (p<0.05) and time x sex (p<0.05). The response increased for male calves with weaning and increased with each sampling time post weaning. For heifers the response was not affected by weaning and plasma concentrations decreased at 168 h post weaning. There was no effect of weaning or sex on leukocyte concentration. The neutrophils : lymphocyte ration increased post weaning (p<0.01) and was affected by sex (p<0.05). Weaning decreased (p<0.05) the in vitro interferon-gamma response to the antigen KLH. There was a time x weaning x sex (p<0.05) interaction for fibrinogen concentration but no effect of treatment on haptoglobin concentration. Abrupt weaning increased plasma cortisol and nor-adrenaline concentrations, which was accompanied by attenuation of in vitro interferon gamma production to novel mitogen and antigen complexes up to 7 days post weaning.European Union Structural Funds (EAGGF

Inflammation in the CNS: advancing the field using intravital imaging

Inflammation of the CNS can have devastating, long-lived, and in some cases fatal consequences for patients. The stimuli that can induce CNS inflammation are diverse, and include infectious agents, autoimmune responses against CNS-expressed antigens, and sterile inflammation following ischemia or traumatic injury. In these conditions, cells of the immune system play central roles in promulgation and resolution of the inflammatory response. However, the immunological mechanisms at work in these diverse responses differ according to the nature of the response. Our understanding of the actions of immune cells in the CNS has been restricted by the difficulty in visualising leukocytes as they undergo recruitment from the cerebral microvasculature and following their entry into the CNS parenchyma. However, advances in in vivo microscopy over the last 10-15 years have overcome many of these difficulties, and studies using these forms of microscopy have revealed a wealth of new information regarding the cellular and molecular mechanisms of CNS inflammation. This Research Topic brings together state of the art reviews examining the use of in vivo imaging in investigating inflammation and leukocyte behaviour in the CNS. Papers in this Research Topic describe how in vivo microscopy has increased our understanding of the actions of immune cells in the inflamed CNS, following various stimuli including autoimmunity, infection and sterile inflammation

A Simulation Study of Space-based Observations of Gravity Waves in the Airglow using Observed ALOHA-93 Wave Parameters

We use gravity wave parameters derived from the ALOHA-93 campaign to model four gravity waves in airglow emissions as observed from the ground to numerically predict whether these waves could have been observed from space. In spite of encountering critical levels, some waves may still be observed in the airglow provided the critical level lies within the airglow emission region. One of the four waves experiences a critical level in the lower region of an airglow layer such that the disturbance to the volume emission rate would be effectively limited to a short distance along a satellite line of sight. The effect of this is to mitigate the effects of destructive interference in the airglow making the wave more observable from space. For this particular wave the amplitude is derived by normalizing the model-derived airglow fluctuation amplitude to that observed from the ground during the ALOHA-93 campaign. The model then provides momentum and energy fluxes as a function of height as well as the flux divergences, from which the mean state forcing is evaluated. The results suggest that the observed wave could provide significant mean state forcing. Therefore, we conclude that some waves experiencing critical level interactions in the airglow regions are not only likely to be important to the momentum balance of the upper mesosphere/lower thermosphere region but also are more likely to be observed from space

Sparse distributed memory prototype: Principles of operation

Sparse distributed memory is a generalized random access memory (RAM) for long binary words. Such words can be written into and read from the memory, and they can be used to address the memory. The main attribute of the memory is sensitivity to similarity, meaning that a word can be read back not only by giving the original right address but also by giving one close to it as measured by the Hamming distance between addresses. Large memories of this kind are expected to have wide use in speech and scene analysis, in signal detection and verification, and in adaptive control of automated equipment. The memory can be realized as a simple, massively parallel computer. Digital technology has reached a point where building large memories is becoming practical. The research is aimed at resolving major design issues that have to be faced in building the memories. The design of a prototype memory with 256-bit addresses and from 8K to 128K locations for 256-bit words is described. A key aspect of the design is extensive use of dynamic RAM and other standard components

Numerical Modeling of the Propagation of Infrasonic Acoustic Waves Through the Turbulent Field Generated by the Breaking of Mountain Gravity Waves

The nonlinear propagation of low-frequency acoustic waves through the turbulent fluctuations induced by breaking mountain gravity waves is investigated via 2-D numerical simulations of the Navier-Stokes equations, to understand the effects of atmospheric dynamics on ground-based infrasound measurements. Emphasis is placed on acoustic signals of frequency around 0.1 Hz, traveling through tens-of-kilometers-scale gravity waves and subkilometer-scale turbulence. The sensitivity of the infrasonic phases to small-scale fluctuations is found to depend on the altitudes through which they are refracted toward the Earth. For the considered cases, the dynamics in the stratosphere impact the refracting acoustic waves to a greater extent than those in the thermosphere. This work clearly demonstrates the need for accurate descriptions of the effects of atmospheric dynamics on acoustic propagation, such as here captured by the full set of fluid dynamic equations, as well as of the subsequent effects on measured signals

Full-wave Modeling of Small-scale Gravity Waves using Airborne Lidar and Observations of the Hawaiian Airglow (ALOHA-93) O(¹S) Images and Coincident Na Wind/temperature Lidar Measurements

Measurements were made of mesospheric gravity waves in the OI (5577 Å) nightglow observed from Maui, Hawaii, during the Airborne Lidar and Observations of Hawaiian Airglow (ALOHA-93) campaign. Clear, monochromatic gravity waves were observed on several nights. By using a full-wave model that realistically includes the major physical processes in this region, we have simulated the propagation of four waves through the mesopause region and calculated the O(¹S) nightglow response to the waves. Mean winds derived from Na wind/temperature lidar observations were employed in the computations. Wave amplitudes were calculated based on the requirement that the observed and simulated relative airglow fluctuation amplitudes be equal. Although the extrinsic (i.e., observed) characteristics of all four waves studied were quite similar (horizontal wavelengths ∼20 to 30 km; periods ∼9 min; horizontal phase speeds ∼35 to 50 m s¯¹), the propagation characteristics of the waves are all quite different due to the different background mean winds through which the waves propagate. Three of the waves encounter critical levels in the mesopause region. For two of these waves the upward propagation beyond the 97 km level is severely impeded by their critical levels because the local value of the Richardson number exceeds unity there. The third wave is not severely attenuated at its critical level because the Richardson number there is about 0.25. The fourth wave does not encounter a critical level although it is strongly Doppler shifted to low frequencies over a limited height range by the mean winds. It appears to be able to propagate at least to the 110 km level essentially unimpeded. This study demonstrates that an accurate description of the mean winds is an essential requirement for a complete interpretation of observed wave-driven airglow fluctuations. The study also emphasizes that although the measured extrinsic properties of waves may be similar, their propagation to higher altitudes depends very sensitively on the mean winds through which the waves propagate

Climatology and Modeling of Quasi-monochromatic Atmospheric Gravity Waves Observed over Urbana Illinois

From analyzing nine months of airglow imaging observations of atmospheric gravity waves (AGWs) over Adelaide, Australia (35°S) [Walterscheid et al., 1999] have proposed that many of the quasi-monochromatic waves seen in the images were primarily thermally ducted. Here are presented 15 months of observations, from February 1996 to May 1997, for AGW frequency and propagation direction from a northern latitude site, Urbana Illinois (40°N). As Adelaide, Urbana is geographically distant from large orographic features. Similar to what was found in Adelaide, the AGWs seem to originate from a preferred location during the time period around summer solstice. In conjunction with these airglow data there exists MF radar data to provide winds in the 90 km region and near-simultaneous lidar data which provide a temperature climatology. The temperature data have previously been analyzed by States and Gardner [2000]. The temperature and wind data are used here in a full wave model analysis to determine the characteristics of the wave ducting and wave reflection during the 15 month observation period. This model analysis is applied to this and another existing data set recently described by Nakamura et al. [1999]. It is shown that the existence of a thermal duct around summer solstice can plausibly account for our observations. However, the characteristics of the thermal duct and the ability of waves to be ducted is also greatly dependent on the characteristics of the background wind. A simple model is constructed to simulate the trapping of these waves by such a duct. It is suggested that the waves seen over Urbana originate no more than a few thousand kilometers from the observation site

The 2009 Samoa and 2010 Chile Tsunamis as Observed in the Ionosphere using GPS Total Electron Content

Ground‐based Global Positioning System (GPS) measurements of ionospheric total electron content (TEC) show variations consistent with atmospheric internal gravity waves caused by ocean tsunamis following two recent seismic events: the Samoa earthquake of 29 September 2009 and the Chile earthquake of 27 February 2010. Both earthquakes produced ocean tsunamis that were destructive to coastal communities near the epicenters, and both were observed in tidal gauge and buoy measurements throughout the Pacific Ocean. We observe fluctuations in TEC correlated in time, space, and wave properties with these tsunamis using the Jet Propulsion Laboratory’s Global Ionospheric Mapping software. These TEC measurements were band‐pass filtered to remove ionospheric TEC variations with wavelengths and periods outside the typical range for tsunamis. Observable variations in TEC appear correlated with the tsunamis in some locations (Hawaii and Japan), but not in others (Southern California or near the epicenters). Where variations are observed, the typical amplitude tends to be ∼0.1–0.2 TEC units for these events, on the order of ∼1% of the background TEC value. These observations are compared to estimates of expected tsunami‐driven TEC variations produced by Embry Riddle Aeronautical University’s Spectral Full Wave Model, an atmosphere‐ionosphere coupled model, and are found to be in good agreement. Significant TEC variations are not always seen when a tsunami is present, but in these two events the regions where a strong ocean tsunami was observed coincided with clear TEC observations, while a lack of clear TEC observations coincided with smaller sea surface height amplitudes. There exists the potential to apply these detection techniques to real‐time GPS TEC data, providing estimates of tsunami speed and amplitude that may be useful for early warning systems

The Importance of extraction protocol on the analysis of novel waste sources of lignocellulosic biomass

peer-reviewedAs the utilization and consumption of lignocellulosic biomass increases, so too will the need for an adequate supply of feedstock. To meet these needs, novel waste feedstock materials will need to be utilized. Exploitation of these novel feedstocks will require information both on the effects of solvent extraction on the succeeding analysis of potential novel feedstocks and how accurate current methodologies are in determining the composition of novel lignocellulosic feedstocks, particularly the carbohydrate and lignin fractions. In this study, the effects of solvent extraction on novel feedstocks, including tree foliage, tree bark and spent mushroom compost, with 95% ethanol, water and both sequentially were examined. Chemical analyses were carried out to determine the moisture content, ash, extractives, post-hydrolysis sugars, Klason lignin (KL) and acid-soluble lignin (ASL) within the selected feedstocks. The result of extraction could be seen most strongly for Klason lignin, with a strong association between higher levels of Klason lignin levels and greater amounts of non-removed extractives (tree foliage and bark). Higher Klason lignin levels are reported to be due the condensation of non-removed extractives during hydrolysis, hence the lower Klason lignin determinations following extraction are more exact. In addition, total sugar determinations were lower following extractions. This is because of the solubility of non-cell-wall carbohydrates; thus, the determinations following extraction are more accurate representations of structural cell-wall polysaccharides such as cellulose. Such determinations will assist in determining the best way to utilize novel feedstocks such as those analyzed in this work

Numerical Simulations of Gravity Waves Imaged Over Arecibo During the 10-Day January 1993 Campaign

Recently, measurements were made of mesospheric gravity waves in the OI (5577 Å) nightglow observed from Arecibo, Puerto Rico, during January 1993 as part of a special 10-day campaign. Clear, monochromatic gravity waves were observed on several nights. By using a full-wave model that realistically includes the major physical processes in this region, we have simulated the propagation of two waves through the mesopause region and calculated the O(1 S) nightglow response to the waves. Mean winds derived from both UARS wind imaging interferometer (WINDII) and Arecibo incoherent scatter radar observations were employed in the computations as were the climatological zonal winds defined by COSPAR International Reference Atmosphere 1990 (CIRA). For both sets of measured winds the observed waves encounter critical levels within the O(1 S) emission layer, and wave amplitudes, derived from the requirement that the simulated and observed amplitudes of the O(1 S) fluctuations be equal, are too large for the waves to be gravitationally stable below the emission layer. Some of the model coefficients were adjusted in order to improve the agreement with the measurements, including the eddy diffusion coefficients and the height of the atomic oxygen layer. The effect of changing the chemical kinetic parameters was investigated but was found to be unimportant. Eddy diffusion coefficients that are 10 to 100 times larger than presently accepted values are required to explain most of the observations in the cases that include the measured background winds, whereas the observations can be modeled using the nominal eddy diffusion coefficients and the CIRA climatological winds. Lowering the height of the atomic oxygen layer improved the simulations slightly for one of the simulated waves but caused a less favorable simulation for the other wave. For one of the waves propagating through the WINDII winds the simulated amplitude was too large below 82 km for the wave to be gravitationally stable, in spite of the adjustments made to the model parameters. This study demonstrates that an accurate description of the mean winds is an essential requirement for a complete interpretation of observed wave-driven airglow fluctuations